high lysine
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2022 ◽  
Author(s):  
Manjari Mishra ◽  
Ray Singh Rathore ◽  
Sneh L Singla‐Pareek ◽  
Ashwani Pareek

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Zahirul A. Talukder ◽  
Vignesh Muthusamy ◽  
Rashmi Chhabra ◽  
Nisrita Gain ◽  
Shashidhar B. Reddappa ◽  
...  

AbstractWaxy maize rich in amylopectin has emerged as a preferred food. However, waxy maize is poor in lysine and tryptophan, deficiency of which cause severe health problems. So far, no waxy hybrid with high lysine and tryptophan has been developed and commercialized. Here, we combined recessive waxy1 (wx1) and opaque2 (o2) genes in the parental lines of four popular hybrids (HQPM1, HQPM4, HQPM5, and HQPM7) using genomics-assisted breeding. The gene-based markers, wx-2507F/RG and phi057 specific for wx1 and o2, respectively were successfully used to genotype BC1F1, BC2F1 and BC2F2 populations. Background selection with > 100 SSRs resulted in recovering > 94% of the recurrent parent genome. The reconstituted hybrids showed 1.4-fold increase in amylopectin (mean: 98.84%) compared to the original hybrids (mean: 72.45%). The reconstituted hybrids also showed 14.3% and 14.6% increase in lysine (mean: 0.384%) and tryptophan (mean: 0.102%), respectively over the original hybrids (lysine: 0.336%, tryptophan: 0.089%). Reconstituted hybrids also possessed similar grain yield (mean: 6248 kg/ha) with their original versions (mean: 6111 kg/ha). The waxy hybrids with high lysine and tryptophan assume great significance in alleviating malnutrition through sustainable and cost-effective means. This is the first report of development of lysine and tryptophan rich waxy hybrids using genomics-assisted selection.


2021 ◽  
Vol 9 (3) ◽  
pp. 199-212
Author(s):  
Ji-Yun Lee ◽  
Ju-Won Kang ◽  
Su-Min Jo ◽  
Young-Ho Kwon ◽  
So-Myeong Lee ◽  
...  
Keyword(s):  

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 618
Author(s):  
Sabry M. El-Bahr ◽  
Saad Shousha ◽  
Mohamed A. Alfattah ◽  
Saad Al-Sultan ◽  
Wasseem Khattab ◽  
...  

This study aimed to evaluate the effect of four combinations of dietary linseed oil and lysine mixtures on performance, fatty and amino acid profiles, oxidative stress biomarkers, cell energy and meat quality parameters of broiler chickens. One hundred and sixty broiler chicks were allocated into four groups. Birds of groups 1–4 were fed diets containing optimum lysine and 2% of linseed oil, optimum lysine and 4% of linseed oil, high lysine and 2% of linseed oil, and high lysine and 4% of linseed oil, respectively, for a period of 35 days. High linseed oil or lysine levels did not affect the performance of the tested birds, but the high level of dietary linseed oil decreased the concentrations of muscles’ saturated fatty acids (SFA). The highest values of ω-3 polyunsaturated fatty (ω-3 PUFA) and arachidonic acids with lowest levels of monounsaturated fatty (MUFA) were detected in the muscles of birds fed diets containing high linseed oils and/or lysine levels. High linseed oil or lysine levels provided the best essential amino acid profile and improved antioxidant components as well as cell energy, and tenderness and redness of the meat. Conclusively, high dietary lysine and linseed oil combinations improved the nutritional value, antioxidant status and cell energy of broiler chickens’ meat.


2020 ◽  
Vol 99 (10) ◽  
pp. 4730-4740
Author(s):  
Jan Jankowski ◽  
Katarzyna Ognik ◽  
Paweł Konieczka ◽  
Dariusz Mikulski

2020 ◽  
Vol 29 (19) ◽  
pp. 3266-3284
Author(s):  
Hilal H Al-Shekaili ◽  
Terri L Petkau ◽  
Izabella Pena ◽  
Tess C Lengyell ◽  
Nanda M Verhoeven-Duif ◽  
...  

Abstract Pyridoxine-dependent epilepsy (PDE) is a rare autosomal recessive disease caused by mutations in the ALDH7A1 gene leading to blockade of the lysine catabolism pathway. PDE is characterized by recurrent seizures that are resistant to conventional anticonvulsant treatment but are well-controlled by pyridoxine (PN). Most PDE patients also suffer from neurodevelopmental deficits despite adequate seizure control with PN. To investigate potential pathophysiological mechanisms associated with ALDH7A1 deficiency, we generated a transgenic mouse strain with constitutive genetic ablation of Aldh7a1. We undertook extensive biochemical characterization of Aldh7a1-KO mice consuming a low lysine/high PN diet. Results showed that KO mice accumulated high concentrations of upstream lysine metabolites including ∆1-piperideine-6-carboxylic acid (P6C), α-aminoadipic semialdehyde (α-AASA) and pipecolic acid both in brain and liver tissues, similar to the biochemical picture in ALDH7A1-deficient patients. We also observed preliminary evidence of a widely deranged amino acid profile and increased levels of methionine sulfoxide, an oxidative stress biomarker, in the brains of KO mice, suggesting that increased oxidative stress may be a novel pathobiochemical mechanism in ALDH7A1 deficiency. KO mice lacked epileptic seizures when fed a low lysine/high PN diet. Switching mice to a high lysine/low PN diet led to vigorous seizures and a quick death in KO mice. Treatment with PN controlled seizures and improved survival of high-lysine/low PN fed KO mice. This study expands the spectrum of biochemical abnormalities that may be associated with ALDH7A1 deficiency and provides a proof-of-concept for the utility of the model to study PDE pathophysiology and to test new therapeutics.


2020 ◽  
Vol 21 (16) ◽  
pp. 5594
Author(s):  
Collins N. Khwatenge ◽  
Boniface M. Kimathi ◽  
Samuel N. Nahashon

Amino acids are known to play a key role in gene expression regulation. Amino acid signaling is mediated via two pathways: the mammalian target of rapamycin complex 1 (mTORC1) and the amino acid responsive (AAR) pathways. Cationic amino acid transporters (CATs) are crucial in these pathways due to their sensing, signaling and transport functions. The availability of certain amino acids plays a key role in the intake of other amino acids, hence affecting growth in young birds. However, the specific mechanism for regulating lysine transport for growth is not clear. In this study, we analyze the transcriptome profiles and mRNA expression of selected cationic amino acid transporters in the livers of broilers fed low and high lysine diets. Birds consumed high-lysine (1.42% lysine) or low-lysine (0.85% lysine) diets while the control group consumed 1.14% lysine diet. These concentrations of lysine represent 125% (high lysine), 75% (low lysine) and 100% (control), respectively, of the National Research Council’s (NRC) recommendation for broiler chickens. After comparing the two groups, 210 differentially expressed genes (DEGs) were identified (fold change >1 and false discovery rate (FDR) <0.05). When comparing the high lysine and the low lysine treatments, there were 67 upregulated genes and 143 downregulated genes among these DEGs. Analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) and the Gene Ontology (GO) enrichment analysis show that cellular growth, lipid metabolism and lysine metabolism pathways were among the significantly enriched pathways. This study contributes to a better understanding of the potential molecular mechanisms underlying the correlation between lysine intake, body weight gain (BWG) and feed intake (FI) in broiler chickens. Moreover, the DEGs obtained in this study may be used as potential candidate genes for further investigation of broiler growth customized responses to individualized nutrients such as amino acids.


2020 ◽  
Vol 98 (5) ◽  
Author(s):  
Lori L Thomas ◽  
Charmaine D Espinosa ◽  
Robert D Goodband ◽  
Hans H Stein ◽  
Mike D Tokach ◽  
...  

Abstract Five experiments were conducted to determine the standardized total tract digestibility (STTD) of P, digestible energy (DE), metabolizable energy (ME), and standardized ileal digestibility (SID) of amino acids (AA) in three sorghum varieties compared with corn and to determine the effects of sorghum varieties on nursery pig growth. In exp. 1, 48 barrows (initially 18.6 kg) were housed individually in metabolism crates. Treatments were arranged in a 2 × 4 factorial evaluating two levels of microbial phytase (0 or 500 units/kg) and four grain sources (corn, high-lysine, red, or white sorghum). Added phytase improved (P &lt; 0.05) STTD of P in all ingredients, but was not different among the grains. In exp. 2, the DE and ME in the three sorghum varieties were not different from corn. In exp. 3, 10 growing barrows (initially 25.9 kg) with a T-cannula in the terminal ileum were used. Standardized ileal digestible Lys, Met, Thr, and Val were greater (P &lt; 0.05) in corn than in the sorghum-based diets with no differences among the sorghum varieties. In exp. 4, 160 pigs (initially 6.3 kg) were randomly allotted to one of four dietary treatments with five pigs per pen and eight replicate pens per treatment in a 20-d experiment. Dietary treatments included corn or the three sorghum varieties, where the varieties of sorghum replaced corn on an SID Lys basis. No differences among treatments were observed in any growth performance parameters. In exp. 5, treatments consisted of a corn-based diet, a diet based on conventional sorghum (a mixture of red and white sorghum), and four diets with high-lysine sorghum containing increasing amounts of feed-grade AA, replacing soybean meal. Overall, pigs fed the high-lysine sorghum diet with the greatest amount of added feed-grade AA had the poorest gain:feed ratio (G:F; P &lt; 0.05) compared with pigs fed all the other experimental diets. Within those fed the high-lysine sorghum and feed-grade AA, average daily gain, final body weight (linear, P &lt; 0.10), and G:F (linear, P &lt; 0.01) decreased as feed-grade AA increased. In summary, no differences in STTD of P or in DE and ME were observed among the grain sources. The SID AA values for the three sorghum varieties were not different; however, they were all lower than for corn. These results indicate that these varieties of sorghum can successfully replace corn in nursery pig diets if diets are formulated to account for differences in AA digestibility.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 173-173
Author(s):  
Charmaine D Espinosa ◽  
Lori L Thomas ◽  
Robert D Goodband ◽  
Hans H Stein

Abstract Two experiments were conducted to test the hypothesis that the standardized total tract digestibility (STTD) of P and digestible energy (DE) and metabolizable energy (ME) in a new variety of high-lysine sorghum is not different from values obtained in conventional sorghum varieties or in corn. In experiment 1, 48 barrows (18.63 ± 0.89 kg) were housed individually in metabolism crates and randomly allotted to 1 of 8 diets. Diets were based on high-lysine sorghum, red sorghum, white sorghum, or corn as the sole source of P and these diets either contained no microbial phytase or 500 units/kg of phytase. Feces were collected using the marker to marker approach with 5-d adaptation and 4-d collection periods. Data were analyzed by ANOVA using the Mixed Procedure of SAS. Results indicated that phytase supplementation improved (P ≤ 0.05) STTD of P in all ingredients, but the STTD of P in high-lysine sorghum was not different from the STTD of P in other grains (Table 1). In experiment 2, 32 barrows (18.54 ± 0.77 kg) were housed individually in metabolism crates and randomly allotted to 1 of 4 diets. Diets contained high-lysine sorghum, red sorghum, white sorghum, or corn as the only energy-containing ingredient. Feces and urine samples were collected using the marker to marker approach with 5-d adaptation and 4-d collection periods. Data were analyzed as explained for Exp. 1. The ATTD of gross energy in high-lysine sorghum was less (P ≤ 0.05) than in corn, but the DE and ME in high-lysine sorghum was not different from corn. In conclusion, supplementation of phytase improved STTD of P in cereal grains, and values for STTD of P, as well as for DE and ME in high-lysine sorghum, were not different from corn, which indicates that high-lysine sorghum can replace corn in swine diets.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 147-148
Author(s):  
China Supakorn ◽  
Clay A Lents ◽  
Xochitl Martinez ◽  
Jeff Vallet ◽  
R Dean Boyd ◽  
...  

Abstract Unacceptable sow longevity in modern production systems has placed nutritional programs for developing gilts into question. Nutritional programs are well defined for terminal pigs, but not for developing gilts. To achieve breeding targets and allow gilts to meet their maternal genetic potential for lifetime productivity, the optimum lysine requirement for developing gilts should be well understood. The National Pork Board Animal Science Committee commissioned trials to determine the optimum lysine levels for developing gilts in U.S. pig production systems. An experiment was conducted to challenge proposed dietary lysine concentration for optimum growth rate during gilt development. A total of 2,960 growing crossbred gilts were fed three grower-finisher diets, formulated to provide low lysine (0.68 and 0.52% SID lysine), medium lysine (0.79 and 0.60% SID lysine), and high lysine (0.90 and 0.68% SID lysine) levels and fed to developing gilts starting from 142 to 200 d of age. Gilts fed the high lysine dietary treatment had significantly increased body weight (BW), flank-to-flank length, backfat thickness (BF), loin depth (LD), average daily gain, and fat-to-lean ratio (P < 0.05). Moreover, gilts fed the high lysine treatment during development phase had greater BW (202.4 ± 1.2 kg), BF (14.8 ± 0.2 mm), and LD (5.4 ± 0.5 cm) at first farrowing and BF (15.1 ± 0.2 mm) at weaning when compared with gilts fed the medium and low lysine treatments (P < 0.05). Litter birth weight (17.4 ± 0.3 kg), number weaned (9.0 ± 0.2), and litter weaning weight (46.6 ± 0.9 kg) from the gilts fed the high lysine sow treatment were greater when compared with performance from gilts fed the medium and low treatments (P < 0.05). Gilt development diets containing 2.79 g and 2.08 g SID lysine per MCal ME (high lysine grower and finisher diets, respectively) allowed gilts to achieve good growth and first parity reproductive performance.


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