PSX-A-20 Late-Breaking: Potential prebiotic effect of canola meal non-starch polysaccharide hydrolysis products in weaned piglets challenged with enterotoxigenic Escherichia coli F4

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 369-370
Author(s):  
Xiaoxiao Zhang ◽  
Bonjin Koo ◽  
Debora Holanda ◽  
Martin Nyachoti ◽  
Jinyoung Lee
Keyword(s):  
Escherichia Coli ◽  
Negative Control ◽  
Enterotoxigenic Escherichia Coli ◽  
Canola Meal ◽  
Experimental Unit ◽  
Adaptation Period ◽  
Weaned Piglets ◽  
Gene Expressions ◽  
Hydrolysis Products ◽  
Polysaccharide Hydrolysis

Abstract Enzymatically derived non-starch polysaccharide hydrolysis products (HP) may modulate enteric health outcomes in piglets. Thus, responses of weaned piglets to an oral challenge with enterotoxigenic Escherichia coli F4 (ETEC) when fed diets containing canola meal (CM) HP were investigated. The HP were obtained by incubating CM with a multi-carbohydrase (MC) blend. Thirty-five weaned piglets (8.08 ± 0.34 Kg BW) were individually assigned in a completely randomized design to 1 of 5 treatments: UCC (unchallenged control), NC (negative control challenged with ETEC), PC (NC + 0.1% AGP), HP1 (NC + 0.25% HP), HP2 (NC + 0.5% HP). After a seven-day adaptation period, piglets in the UCC group received 5 ml of PBS, whereas those in all other groups were orally challenged with 5 ml (5 × 106 CFU/mL) of ETEC F4. On d 14 all piglets were euthanized for tissue sampling. Data were analyzed using the PROC MIXED procedure of SAS 9.4, with the individual pig as the experimental unit. During the post-challenge period, piglets fed NC diet had lower (P < 0.05) G: F than those fed the PC, whereas the G: F was higher (P < 0.05) for piglets fed the HP-containing diets than that of piglets fed the NC diet. Piglets fed diets containing HP showed lower (P < 0.05) jejunum pH and fecal scores than piglets fed the NC diet. For gene expressions, the HP groups showed a greater level of toll like receptor 2 (TLR2) compared with NC group (P < 0.05). For the HP groups, HP1 had a higher (P < 0.05) level of TLR2 than HP2 treatment. Feeding HP resulted in lower relative abundance of Bifidobacterium in the colon of piglets compared with feeding with NC (P < 0.05). In conclusion, piglets fed diets with CM HP exhibited less severe ETEC-enteritis and had similar effects with AGP.

Gastrointestinal ecology response of piglets’ diets containing non-starch polysaccharide hydrolysis products and egg yolk antibodies following an oral challenge with Escherichia coli (k88)

2009 ◽  
Vol 89 (3) ◽  
pp. 341-352 ◽  
Author(s):  
E Kiarie ◽  
B A Slominski ◽  
D O Krause ◽  
C M Nyachoti
Keyword(s):  
Escherichia Coli ◽  
Egg Yolk ◽  
Intestinal Morphology ◽  
Oral Challenge ◽  
Enterotoxigenic Escherichia Coli ◽  
Adaptation Period ◽  
Egg Yolk Antibodies ◽  
Hydrolysis Products ◽  
K88 Fimbriae ◽  
Polysaccharide Hydrolysis

The gastrointestinal ecology (GE) of piglets fed diets containing non-starch polysaccharide hydrolysis products (HP) and egg yolk antibodies against K88 fimbriae (EYA) following oral challenge with enterotoxigenic Escherichia coli K88 (ETEC) was investigated. The HP were products of incubating feedstuffs with a blend of carbohydrase enzymes. Forty, 21-d-old pigs (two pigs/pen) were assigned to four diets to give five pens per diet. The diets were: a control fed without or with 5 g kg-1 of HP and EYA either singly or in combination forming a 2 × 2 factorial arrangement. Following a 9-d adaptation period, all pigs were orally challenged with ETEC and killed at 24 and 48 h post-challenge (one pig/pen on each occasion). Feeding HP increased pre-challenge average daily gain (252 vs. 207 g d-1; P = 0.01). An interaction (P < 0.10) between EYA and HP was observed such that when fed in combination they resulted in higher ileal digesta lactic acid and cecal DM contents and lower ileal digesta ammonia. The main effects (P < 0.05) were such that pigs fed EYA-diets had shorter intestinal crypt whilst pigs fed HP-diets showed low gastric pH and high ileal mucosal adherent lactobacilli counts. In conclusion, HP and EYA influenced indices of fermentative characteristics and intestinal morphology in the gastrointestinal ecology of piglets orally challenged with enterotoxigenic E. coli (k88).Key words: Egg yolk antibodies, ETEC, gastrointestinal ecology, non-starch polysaccharides hydrolysis products, piglet

Acute phase response of piglets fed diets containing non-starch polysaccharide hydrolysis products and egg yolk antibodies following an oral challenge with Escherichia coli (k88)

2009 ◽  
Vol 89 (3) ◽  
pp. 353-360 ◽  
Author(s):  
E Kiarie ◽  
B A Slominski ◽  
D O Krause ◽  
C M Nyachoti
Keyword(s):  
Escherichia Coli ◽  
Acute Phase ◽  
Egg Yolk ◽  
Oral Challenge ◽  
Enterotoxigenic Escherichia Coli ◽  
Adaptation Period ◽  
Post Challenge ◽  
Egg Yolk Antibodies ◽  
Hydrolysis Products ◽  
K88 Fimbriae

Acute phase responses (APR) in pigs fed non-starch polysaccharides hydrolysis products (HP) and egg yolk antibodies against K88 fimbriae (EYA) following oral challenge with enterotoxigenic Escherichia coli K88 (ETEC) were evaluated. The HP were products of incubating feedstuffs with a blend of carbohydrase enzymes. Forty, 21-d-old pigs (two pigs/pen) were assigned to four diets to give five pens per diet. The diets were: a control fed without or with 5 g kg-1 of HP and EYA either singly or in combination forming a 2 × 2 factorial arrangement. Following a 9-d adaptation period, pigs were bled and given an oral dose of ETEC. Pigs were then bled and feed intake recorded within 48 h post-challenge. Pigs fed HP ate more (P < 0.05) than pigs not fed HP during the ETEC challenge. Interaction between additives and time was observed for packed cell volume (PCV, P = 0.0002), in which case pigs fed the control diet showed lower PCV at 6 h post-challenge than pigs fed additives. At 48 h post-challenge, main effects (P < 0.10) were such that pigs fed HP-diets had lower serum haptoglobin and pigs fed EYA-diets had higher interleukin-6 compared with pigs fed non-HP and non-EYA diets, respectively. In conclusion, HP and EYA reduced the severity of ETEC-enteritis in piglets with some evidence of synergistic effects. Key words: Acute phase responses, egg yolk antibodies, non-starch polysaccharides, hydrolysis products, piglet diarrhea

PSX-A-11 Late-Breaking: Effects of phytogenic supplementation on growth performance and intestinal barrier function in nursery pigs challenged with E. coli

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 372-374
Author(s):  
Rachita Maniyar ◽  
Bonjin Koo ◽  
Debora Holanda ◽  
Chengbo Yang ◽  
Karmin O ◽  
...  
Keyword(s):  
Ussing Chamber ◽  
Average Daily Gain ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Barrier Function ◽  
Experimental Unit ◽  
Post Inoculation ◽  
Gut Health ◽  
Weaned Piglets ◽  
Health Promoting ◽  
Fecal Score

Abstract The objective of this study was to investigate the health-promoting effects of red osier dogwood (ROD) extract as an alternative to antibiotics in weaned piglets challenged with enterotoxigenic Escherichia coli (ETEC). Twenty-eight weaned piglets (9.15±0.95 kg BW) confirmed to genetically susceptible to ETEC were individually assigned to one of the four dietary treatments in a completely randomized design. Experimental diets were, negative control (NC), corn-wheat soybean meal diet with no additives; positive control (PC), NC plus antibiotics; ROD1, NC plus 0.1% ROD extract; ROD2, NC plus 0.2% ROD extract. Piglets were orally challenged on d 7 with ETEC F4. Feed disappearance, body weight, fecal score, and rectal temperature were recorded. On d 14, piglets were euthanized to collect intestinal tissue samples for histomorphology and Ussing chamber analysis. Data were analyzed using the MIXED procedure of SAS using individual piglet as the experimental unit. There were no differences (P &gt; 0.10) in histomorphology and intestinal permeability. Piglets fed the NC diet tended (P &lt; 0.10) to have higher average daily gain, post-inoculation than those fed ROD1 or ROD2. Fecal score of piglets fed the PC diet tended to (P &lt; 0.10) or was significantly lower (P &lt; 0.05) than for piglets fed ROD1 or ROD2 on 0 and 2 days post-inoculation (dpi), respectively. On 0 dpi, piglets fed the ROD1 diet had significantly higher (P &lt; 0.05) body temperature than those fed PC or ROD2. In conclusion, ROD extract supplementation might have some health-promoting effects on ETEC challenged piglets but could not improve gut health to the same extent as antibiotics.

The effect of enterotoxigenic Escherichia coli F4ab,ac on early-weaned piglets: A gene expression study

2013 ◽  
Vol 152 (1-2) ◽  
pp. 87-92 ◽  
Author(s):  
M. Schroyen ◽  
B.M. Goddeeris ◽  
A. Stinckens ◽  
R. Verhelst ◽  
S. Janssens ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Gene Expression Study ◽  
Weaned Piglets ◽  
Expression Study

scholarly journals 150 A bio-active, mineral-based feed additive improved growth performance and reduced severity of diarrhea in weanling pigs challenged with enterotoxigenic Escherichia coli

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 84-85
Author(s):  
Sue Sinn ◽  
Ran Song ◽  
Dana Beckler ◽  
Rob Musser ◽  
Kim Friesen
Keyword(s):  
Escherichia Coli ◽  
Growth Performance ◽  
Meta Analysis ◽  
Enterotoxigenic Escherichia Coli ◽  
Feed Additive ◽  
Post Inoculation ◽  
Adaptation Period ◽  
Weanling Pigs ◽  
Serum Samples ◽  
E Coli

Abstract A mineral-based feed additive, NutriQuest Protect™, was evaluated in five artificial Enterotoxigenic Escherichia coli (ETEC) challenge experiments to determine the effects on pig growth performance, fecal consistency and immune response. The five experiments were conducted following a similar procedure and utilized a total of 232 weanling pigs (19 d of age) assigned to one of three experimental treatments: non-challenged control (NC), challenged control (CC), and challenged pigs fed Protect at 4.0 g/kg (CP) with 36, 36, and 44 pens per treatment, respectively. Pharmacological ZnO or medications were not included in any diets. Pigs were allowed a 7-d adaptation period following weaning, orally inoculated with E. coli K88 or F18 on 0 d post-inoculation (dpi) and 1-dpi. Studies were concluded on 4-dpi. Pig BW and feed disappearance were measured on 0-dpi and 4-dpi. Serum samples were collected on 0 and 4-dpi to measure porcine proinflammatory cytokines. Fecal scores were measured daily over the challenge period. Data from the five experiments were compiled for meta-analysis using the MIXED procedure of SAS. The NC pigs had a greater ADG (0.09 vs. -0.01 kg/d, P = 0.002), ADFI (0.24 vs. 0.21 kg/d, P = 0.09), and final BW (6.8 vs. 6.5 kg, P < 0.05). Diarrhea frequency was significantly higher in CC pigs compared with pigs on CP and NC treatments (28.5 vs. 18.7 vs. 5.3%, P < 0.05). Results from the five experiments suggest that NutriQuest Protect™ improves growth performance and reduces inflammation and diarrhea in weaned pigs artificially challenged with E. coli K88 or F18.

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