Long-Term In Vitro Cultivation of Some Mouse Ascites Tumors: Ehrlich Ascites Carcinoma23

Rna Polymerases ◽

Ehrlich Carcinoma ◽

Ehrlich Ascites ◽

Mouse Ascites ◽

Ascites Tumors

Nuclear DNA-dependent RNA polymerases were isolated from Ehrlich ascites carcinoma, TA3 ascites adenocarcinoma, and mouse liver and tested for inhibition by glycerol. The results confirm the finding of Smith and Duerksen ((1975) Biochem. Biophys. Res. Commun. 67, 916–923) that glycerol may inhibit nuclear RNA polymerase II, but because different grades of glycerol inhibited mouse liver RNA polymerase IIa to different extents, it is suggested that an inhibitory contaminant is present. RNA polymerases IIa and IIb from the two tumors and mouse liver were proportionately inhibited by A.C.S. reagent-grade glycerol at concentrations above 10%. RNA polymerase Ia from liver and the TA3 tumor was not inhibited by any concentration of glycerol tested (2–32.3%), but RNA polymerase Ia from Ehrlich carcinoma was inhibited by glycerol concentrations above 16%.

FREQUENCY DISTRIBUTION OF STAGES OF THE CELL CYCLE OF MOUSE ASCITES TUMORS FOLLOWING IN VIVO AND IN VITRO LABELING WITH TRITIATED THYMIDINE

Canadian Journal of Genetics and Cytology ◽

10.1139/g69-026 ◽

1969 ◽

Vol 11 (1) ◽

pp. 217-226

Author(s):

S. B. Hrushovetz

Keyword(s):

Cell Cycle ◽

Frequency Distribution ◽

Direct Method ◽

Tritiated Thymidine ◽

Single Intraperitoneal Injection ◽

Ehrlich Ascites ◽

Mouse Ascites ◽

Ascites Tumors

Ehrlich Ascites tumor cells aspirated from the peritoneal cavity of mice bearing 7-10-day-old tumors when incubated for 1 hour at 37 °C in medium 199 containing tritiated thymidine at a concentration of 4 μc/ml. yielded labeling indices and frequency distribution of stages of the cell cycle similar to those obtained when the same mice received a single intraperitoneal injection of 100 μc of the label and the cells were harvested 1 hour later. Analysis of stages of the cell cycle by the combined techniques of DNA cytophotometry and cellular autoradiography (direct method) yielded a frequency distribution of S, G2, M, and G1, similar to those of other investigators with the labeled metaphase curve (indirect) method.

THE EHRLICH ASCITES CARCINOMA AS A TOOL FOR THE RAPID EVALUATION OF THE EFFICACY OF RADIOPROTECTIVE COMPOUNDS AT THE CELLULAR LEVEL

10.1139/o64-140 ◽

1964 ◽

Vol 42 (9) ◽

pp. 1307-1316 ◽

Cited By ~ 3

Author(s):

J. F. Scaife

Keyword(s):

Quantitative Method ◽

Cellular Level ◽

Proliferative Capacity ◽

Ascites Tumor ◽

Anaerobic Conditions ◽

Ehrlich Ascites ◽

Mouse Ascites

A simple, rapid quantitative method for biologically evaluating radioprotective compounds at the cellular level has been developed by means of Ehrlich mouse ascites tumor cells. The cells were irradiated in vitro and subsequently grown in vivo and the radioprotective compound was evaluated by its ability to prevent mitotic arrest and loss of proliferative capacity in the cells. Results statistically significant at the 1% level were available in 48 hours when groups of 15 mice per sample were compared. L-Cysteine was found to be a better protector than 2-aminoethylisothiouronium bromide hydrobomide or 2-mercaptoethylamine and also increased the protection of cells irradiated under anaerobic conditions.

Determination of in vitro antioxidant activity and in vivo antineoplastic effects against Ehrlich ascites carcinoma of methanolic extract of Sphagneticola calendulacea (L.) Pruski

Heliyon ◽

10.1016/j.heliyon.2021.e07228 ◽

2021 ◽

pp. e07228

Author(s):

Md. Wasim Bari ◽

Ariful Islam ◽

Md. Monirul Islam ◽

Mst Julia Sultana ◽

Rashida Afroz ◽

...

Keyword(s):

Antioxidant Activity ◽

Methanolic Extract ◽

Ehrlich Ascites ◽

Sphagneticola Calendulacea ◽

In Vitro Antioxidant Activity

THE EFFECT OF ACTINOMYCIN D ON THE INCORPORATION OF C14-FORMATE INTO NUCLEIC ACIDS OF EHRLICH ASCITES CARCINOMA AND NOVIKOFF HEPATOMA IN VITRO

10.1139/o64-066 ◽

1964 ◽

Vol 42 (4) ◽

pp. 563-566 ◽

Cited By ~ 1

Author(s):

S. H. Zbarsky

Keyword(s):

Nucleic Acids ◽

Actinomycin D ◽

Novikoff Hepatoma ◽

Ehrlich Ascites

Tumor cell metabolism in vitro: a study of incubation media

10.1139/o70-083 ◽

1970 ◽

Vol 48 (4) ◽

pp. 517-519 ◽

Cited By ~ 2

Author(s):

I. C. Caldwell ◽

Marianne F. Chan

Keyword(s):

Nucleic Acid ◽

Structural Integrity ◽

Cell Metabolism ◽

Leukemic Cells ◽

Prolonged Incubation ◽

Ehrlich Ascites ◽

Eac Cells ◽

Rna And Dna

A number of incubation media which have been used in studies of the metabolism of Ehrlich ascites carcinoma (EAC) cells in vitro have been examined with respect to their abilities to support the incorporation of radioactive precursors into nucleotides and nucleic acids, and to maintain the structural integrity and tumor-inducing abilities of EAC cells. Cells incubated in the chemically-defined "Fischer's medium for leukemic cells of mice" were able to produce lethal tumors in mice after more than 16 h of incubation, maintained their structural integrity on prolonged incubation, and catalyzed high rates of incorporation of exogenously added substrates into nucleotides, RNA, and DNA. However, cells incubated in balanced salts solutions supplemented with glucose had these characteristics: (a) were unable to produce lethal tumors after 4 h of incubation, (b) released large amounts of nucleotide, nucleic acid, and protein material into the medium after less than 2 h of incubation, and (c) catalyzed the incorporation of radioactive precursors into nucleotides and RNA at much lower rates than did cells incubated in Fischer's medium, and were virtually unable to catalyze the incorporation of adenine-14C into DNA.

STUDIES ON THE BIOSYNTHESIS OF NUCLEOTIDES IN EHRLICH ASCITES CARCINOMA CELLS IN VITRO

10.1139/o65-028 ◽

1965 ◽

Vol 43 (2) ◽

pp. 209-224 ◽

Cited By ~ 7

Author(s):

B. I. Uppin ◽

P. G. Scholefield

Keyword(s):

Exchange Reaction ◽

Carcinoma Cells ◽

The Other ◽

Metabolic Inhibitors ◽

Ehrlich Ascites ◽

Oxidative Pathway ◽

Time Courses ◽

Oxidation Of Glucose

Studies have been made of the effects of metabolic inhibitors on the oxidation and incorporation of radioactivity into nucleotides of glucose labelled in the 1, 2, and 6 positions. The results indicate that in Ehrlich ascites carcinoma cells the predominant oxidative pathway is the hexosemonophosphate shunt. Investigation of the time courses of oxidation of the labelled glucose molecules confirms this conclusion. The pattern of incorporation of radioactivity initially suggests that nucleotide ribose is not formed via this pathway. However, it is shown that the coupling of an active transketolase system with the other enzymes of the hexosemonophosphate shunt provides a sufficient explanation of all the experimental observations. The conclusion is reached that pentose is formed by oxidation of glucose through the shunt but that the labelling pattern is largely established as the result of the exchange reaction catalyzed by transketolase.

In vitro anticancer activity of Astaxanthin

World Journal of Biology Pharmacy and Health Sciences ◽

10.30574/wjbphs.2021.5.3.0110 ◽

2021 ◽

Vol 5 (3) ◽

pp. 033-037

Author(s):

Uma Nath U ◽

Ravi. R ◽

Sundara Ganapathy ◽

Lal Prasanth

Keyword(s):

Anticancer Activity ◽

Tumor Volume ◽

Hematological Parameters ◽

High Dose ◽

Ehrlich Ascites ◽

Shrimp Shell ◽

Shrimp Shell Waste ◽

Wbc Count

This study was designed to determine the in vitro anticancer potential of the Astaxanthin isolated from shrimp shell waste (ETC) against Ehrlich Ascites Carcinoma (EAC) induced cancer in swiss albino mice. The anticancer activity was assessed using in vitro cytotoxicAity, mean survival time, tumor volume and hematological studies. The reliable criteria for evaluating the potential of any anticancer agent is the prolongation of lifespan of the animal and decrease in WBC count of blood. The high dose of ETC (200 mg/kg, orally) significantly reduced the tumor growth which was demonstrated by increased lifespan of the mice and restoration of hematological parameters. ETC was also found to be cytotoxic in the in vitro parameter which shows that ETC possesses significant anticancer potential.

In vitro and in vivo antitumor activity of deoxyelephantopin from a potential medicinal plant Elephantopus scaber against Ehrlich ascites carcinoma

Biocatalysis and Agricultural Biotechnology ◽

10.1016/j.bcab.2019.101106 ◽

2019 ◽

Vol 19 ◽

pp. 101106 ◽

Cited By ~ 1

Author(s):

Farha Arakkaveettil Kabeer ◽

Dhanya Sethumadhavan Rajalekshmi ◽

Mangalam Sivasankaran Nair ◽

Remani Prathapan

Keyword(s):

Antitumor Activity ◽

Medicinal Plant ◽

In Vivo Antitumor Activity ◽

Ehrlich Ascites ◽

Elephantopus Scaber

In vitro and in vivo evaluation of antitumor activity of methanolic extract of Argyreia nervosa leaves on Ehrlich ascites carcinoma

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v10i2.22334 ◽

2015 ◽

Vol 10 (2) ◽

pp. 399

Author(s):

Bhawna Sharma ◽

Isha Dhamija ◽

Sandeep Kumar ◽

Hema Chaudhary

Keyword(s):

Antitumor Activity ◽

Solid Tumor ◽

Methanolic Extract ◽

Ehrlich Ascites ◽

Wide Range ◽

Antioxidant Parameters ◽

Argyreia Nervosa

The herb of importance like Argyreia nervosa has shown wide range of pharmacological activities. Its methanolic extract of A. nervosa has been explored against Ehrlich ascites carcinoma (EAC) induced liquid and solid tumor in mice. Liquid and solid tumors were induced by intraperitoneal and subcutaneous transplantation of EAC cells in Balb/C mice. Significant and dose dependant results are observed when the mice are sacrificed on 15th day for estimation of tumor proliferation, hematological, biochemical and hepatic antioxidant parameters. Mean survival time (days) was increased to 36.5 from 20.5 extract treated mice. The extract also showed a decrease (p<0.001) in body weight and percentage reduction in tumor volume respectively when it was evaluated in solid tumor induced mice for a period of 30 days. From the result it was concluded that the extract has as a potent antitumor activity and that is comparable to 5-fluorouracil.