scholarly journals Disruption of MIR396e and MIR396f improves rice yield under nitrogen-deficient conditions

2019 ◽  
Vol 7 (1) ◽  
pp. 102-112 ◽  
Author(s):  
Jinshan Zhang ◽  
Zhenyu Zhou ◽  
Jinjuan Bai ◽  
Xiaoping Tao ◽  
Ling Wang ◽  
...  

Abstract The microRNA miR396 directly represses GROWTH-REGULATING FACTORs (OsGRFs) and has been implicated in regulating rice yield and in nitrogen assimilation. Overexpressing the miR396 targets OsGRF4 and OsGRF6 improves rice yield via increased grain size and panicle branching, respectively. Here, we used CRISPR/Cas9 to assess the function of miR396 genes in rice. Knockout of MIR396ef (MIR396e and MIR396f), but not other isoforms, enhanced both grain size and panicle branching, resulting in increased grain yield. Importantly, under nitrogen-deficient conditions, mir396ef mutants showed an even higher relative increase in grain yield as well as elevated above-ground biomass. Furthermore, we identified OsGRF8 as a new target of miR396, in addition to the known targets OsGRF4 and OsGRF6. Disruption of the miR396-targeting site in OsGRF8 was sufficient to both enlarge grain size and elongate panicles. Our results suggest that rice-seed and panicle development are regulated by miR396ef-GRF4/6/8-GIF1/2/3 modules and that miR396ef are promising targets of genome editing for breeding environmentally friendly rice varieties that require less nitrogen fertilization.

2016 ◽  
Vol 4 (2) ◽  
pp. 223-227
Author(s):  
S. Ranabhat ◽  
L.P. Amgain

A field experiment was conducted on farmer’s field at two sites of Lamjung district of Nepal viz. Bhotewodar and Sundarbazaar to evaluate the performance of two commonly grown rice varieties viz US-382(hybrid) and Ramdhan (improved) under two nutrient management practices [Nutrient Expert®(NE) rice model recommendation, and government recommendation(GR)].Four replicates of four treatments were arranged in randomized completely block design. Rice varieties responded differently under the different nutrient management practices in terms of plant height, grains per panicle, sterility%, panicle weight, grain yield at 15% moisture, straw yield and harvesting index. The highest grain yield was obtained from NE field of US-382 variety which was followed by GR for US-382, NE for Ramdhan and GR for Ramdhan variety. NE based practices for US-382 variety produced higher biological yield as compared to GR. NE estimated attainable rice yield provided by the software compared with actual rice yield from the trials in farmer’s field and NE-based fertilizer recommendations proved the validity in reaching the yield targets estimated by the software. The observed rice yields recorded in the trials were higher than the NE estimated attainable yields, so NE recommendation for US-382 variety was found better over GR.Int J Appl Sci Biotechnol, Vol 4(2): 223-227


2017 ◽  
Vol 4 (3) ◽  
pp. 94-101
Author(s):  
Sugiyanta Sugiyanta ◽  
Galih Angga Kusuma

The new plant type of rice, or ‘padi tipe baru’ (PTB) in Indonesian, is a new group of rice cultivars with a higher productivity compared to the modern rice varieties (‘varietas unggul baru’ or VUB). This study was conducted to determine the optimum dose of nitrogen fertilization and the seedling density or number of seedlings per hill to increase growth and yield of the new type of rice “IPB 3”. The experiment was conducted in the Bogor Experimental Station  in January to June 2015.  Nitrogen doses (90, 120, 150, 180 kg N.ha-1) and number of seeds per hill (1, 5, 10, 15 and 20) were tested.  The results showed that the increased dose of nitrogen fertilizer reduced the percentage of filled grains, the rate of photosynthesis and the grain yield per hectare. Similarly, an increase in the seedling density up to 15 seeds decreased the dry matter of the crop, the number of grains per panicle, and grain weight per plant, but increased the grain yield per hectare. The optimum yield of “IPB 3S” was achieved with  90 kg N.ha-1 with 15 seeds per hill. The results of this study have provided a guideline for an improved nitrogen management in relation to rice seedling population to increase yield of a new rice type “IPB3S”.Keywords:  “IPB 3S”, new plant type, fertilizer, local variety


2020 ◽  
Vol 23 (1) ◽  
pp. 65-72
Author(s):  
A Biswas ◽  
M M E Ahmed ◽  
T Halder ◽  
S Akter ◽  
R Yasmeen ◽  
...  

Photosensitive rice varieties have higher advantages in delayed planting. The present study was undertaken to figure out the degrees of delayed planting and evaluate the performance of six modern Aman rice varieties (BR11, BR22, BR23, BRRI dhan46, BRRI dhan54) against three sets of delayed planting. Each rice variety showed considerable variations for plant height, days to panicle initiation, flowering and maturity. BR22, BR23, and BRRI dhan54 showed a drastic reduction in days to panicle initiation and varied from 65 to 58 days, 69 to 60 days, and 62 to 55 days respectively, while it varied from 76 to 80 days for the control variety BR11. Similarly, days to flowering of BR22 and BRRI dhan54 showed a gradual decrease following different planting time and varied from 92 to 86 days, and 83 to 77 days respectively, while it varied from 109 to 107 days for the control variety BR11. For the above traits, BRRI dhan44 and BRRI dhan46 showed a moderate reduction depending on the three sets of planting time. Grain yield of BR22, BR23 reduced at the third set with a value ranging from 5.8 to 5.0 t/ha, 6.1 to 5.1 t/ha respectively, while BRRI dhan54 showed consistency in grain yield with a range from 5.3 to 5.2 t/ha. In contrast, grain yield of remaining rice varieties ranged between 5.7 to 4.5 t/ha for BRRI dhan46, 6.2 to 3.9 t/ha for BRRI dhan46 while severe reduction for the control variety BR11 with a range from 4.4 to 2.0 t/ha was observed. In conclusion, BRRI dhan54 was found more suitable rice variety in delayed planting compared with other photosensitive rice varieties. In future, BRRI dhan54 could be used as benchmark rice variety in a special rice breeding programme designed for delayed planting. Bangladesh Rice j. 2019, 23(1): 65-72


2017 ◽  
Vol 51 (05) ◽  
Author(s):  
Pragyan Kumari ◽  
P. Vijay Kumar ◽  
Ramesh Kumar ◽  
A. Wadood ◽  
Deepak Anuranjan Tirkey

The study was conducted at Birsa Agricultural University, Ranchi during the years 2009-2013 for understanding the effect of weather variables on the yield of three direct seeded upland rainfed rice varieties viz., Vandana, BVD-109 and BVD-111 in Jharkhand. Correlation and regression methods were used to estimate the impacts of weather on rice yield. Study indicated that temperature, rainfall and sunshine hours have significant effect on the rice yield. Average minimum temperature during sowing to vegetative stage in cv. Vandana and during flowering in BVD-109 and BVD-111 showed significant negative relationship with yield. Rainfall during vegetative and grain filling stage are significantly and positively related with grain yield in all the three rice varieties. The effect of sunshine hours on grain yield of rice was comparatively higher at milk stage than the other phenological stages in all three varieties. However, the effect of sunshine hours on yield is significant during the milk stage in Vandana and BVD-111 only. Multiple regressions using critical weather parameters in critical stages of the three varieties could explain the variability in yield of Vandana, BVD-109 and BVD-111 by 92, 74 and 91 percent, respectively.


2020 ◽  
pp. 1004-1010
Author(s):  
Gribaldi Gribaldi ◽  
Nurlaili Nurlaili ◽  
Firnawati Sakalena ◽  
Nurmala Dewi ◽  
Ardi Asroh

This study aims to determine the effect of regulating the provision of nitrogen fertilizer on several rice varieties on the growth and yield of rice in ratoon system at the Tidal swampland. A split plot design was employed in this experiment. The main plot was nitrogen fertilizer application (N) consisting of N1, N2, N3, and N4. The subplot is rice varieties (V) consisting of Inpari 30 (V1), Inpara 3 (V2), Inpari 33 (V3), Inpari 43 (V4) and Hipa 5 Ceva (V5). The results showed that ½ dose nitrogen fertilization given at planting + ½ dose at primordia had a good effect on the growth and yield of the main crop, whereas 1/3 dose nitrogen fertilization given at planting + 1/3 dose at primordia + 1/3 dose at harvest tends to have a good effect on ratoon yield. Variety Hipa 5 Ceva produced highest yield of unhusked rice (i.e. 4.9 tons ha-1 for the main crop and 2.71 tons ha-1 for ratoon) at (N3): 135 kg N ha-1 fertilizer, when ½ dose was given at planting + ½ dose at primordia. The variety Hipa 5 Ceva with various nitrogen fertilization strategies provided the highest total grain yield in the ratoon system at tidal swampland.


2020 ◽  
Vol 9 (11) ◽  
pp. e6779119409
Author(s):  
Samuel Ferrari ◽  
Luis Fernando dos Santos Cordeiro ◽  
Luís Guilherme Delovo Carara ◽  
Vagner do Nascimento ◽  
Paulo Renato Matos Lopes ◽  
...  

Trinexapac-ethyl spraying time and proper nitrogen rate are essential to reduce plant height and lodging at harvest, without affecting rice yield and nutritional balance. This study aimed to evaluate trinexapac-ethyl spraying time and N contents as topdressing for upland rice paddies. The experiment was carried out in a randomized block design and a 4x5 factorial scheme, with four replications. Treatments consisted of four trinexapac-ethyl spraying times, in the phenological stages of tillering, floral differentiation, between tillering and floral differentiation, and a control (without spraying), and five nitrogen rates (0, 50, 100, 150, and 200, kg ha-1) as topdressing. As nitrogen topdressing rates increased, leaf contents of N, Fe++, and Zn++ increased, while S and Mn++ decreased; however, contents of P, Ca++, Mg++, B, and Cu++ were little influenced. When trinexapac-ethyl was applied, leaf contents of N, P, S, B, and Zn++ were little influenced, while P, Ca++, Mg++, Fe++, and Mn++ increased, and Cu++ decreased. Trinexapac-ethyl can be applied at tillering without decreasing rice yield. Upland rice increased grain yield by 58 and 46% in two consecutive crop years due to application of about 120 kg N ha-1 as topdressing.


2018 ◽  
Vol 19 (2) ◽  
pp. 83 ◽  
Author(s):  
Susilawati Susilawati ◽  
Bambang Sapta Purwoko

<p>Tidal swampland in Indonesia is potential for cultivation of inbred and hybrid rice. However, rice cultivation in this land can be done only one time annually. Rice cultivation with ratoon system in this land might increase production from both the main crop and their ratoon. The study aimed to evaluate the ability of hybrid and inbred rice to produce ratoon and their grain yield in the tidal swampland of Central Kalimantan. The experiment was arranged in a randomized block design with 12 rice varieties as treatments, namely six hybrids (Hipa 3, Hipa 4, Hipa 5, Hipa 6, Maro and Rokan) and six inbreds (Batanghari, Ciherang, IR42, Margasari, Mekongga and Sintanur); replicated three times. Cultivation of the main crop followed the recommendation. At harvest, the main crops were cut at 20 cm from the ground and fertilized with 100 kg ha-1 urea. The results showed that ratoon crops were emerged at 5–6 days after cutting, with 2–4 leaves per panicle and 5.5–26.0 tillers per clump. Average harvesting age of the ratoon was 69 days. Ratoon yield of the hybrids was better than that of the inbreds; the average was 75.2% of the main crops. Based on the criteria of rice yield, three clusters were identified, i.e. high (&gt;2 t ha-1): Hipa 3, Hipa 4, Hipa 5, Maro, Rokan, Ciherang and Sintanur; medium (1–2 t ha-1): Hipa 6, IR42, Margasari and Mekongga, and low, Batanghari. The study suggests that hybrid varieties could be developed in tidal swamplands.</p>


1992 ◽  
Vol 19 (2) ◽  
pp. 185 ◽  
Author(s):  
SK Sahu ◽  
PK Mohapatra

Growth, spikelet number and assimilate contents of the panicle on the mainshoot of 20 indica rice varieties consisting of four duration groups were measured at intervals during the period between 2 weeks prior to anthesis and maturity. Panicle development was of longer duration in the late maturing varieties than in the early, and grain yield of the former was more than that of the latter due to difference in grain number. Shifting the cultivation of an early duration check variety to match the time of reproductive development in the medium duration group did not change its pattern of development. During development, the concentration of soluble carbohydrates, amino acids and phosphates changed significantly with time, and the pattern was the same in all varieties. Initially the concentration was low, but increased sharply a few days before anthesis to a peak level, then declined until anthesis. After anthesis it decreased to a minimum at maturity. Pre-anthesis metabolite concentration of the panicle was higher in the late varieties than in the early varieties, but not much difference was observed post-anthesis. Growth durations of the vegetative, reproductive and grain filling periods collectively influenced grain yield but, on the basis of partial correlation analysis, the vegetative duration was found most important for yield.


Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Rudy Soplanit ◽  
Siti H Nukuhaly

This study was conducted in Waelo Village, Waeapo Subdistrict, Buru District, with the objectives to determine N fertilizer application timing to increase rice yield and to understand the response of two rice varieties commonly grown in Waeapo. The experimental method used in this study was a Randomized Complete Block Design, with several fertilization treatments (NPK, NK,NP, PK and control), and rice varieties (Membramo and Mekongga), and with three replications. The results showed that administration of N fertilizer (NP, NK, NPK) gave significant effects on plant height, leaf color, full grain number per panicle, 1000 grain weight, dry grain yield, soil N content and leaf N content;  meanwhile it did not affect soil reaction (pH). A low rice yield was obtained in the treatment without N fertilizer, with 2.50 kg dry grain yield per plot and PK treatment with 2.78 kg  dry grain yield per plot.


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