scholarly journals JC Virus Granule Cell Neuronopathy and Lymphoma

2018 ◽  
Vol 5 (6) ◽  
Author(s):  
Alexis Demas ◽  
Omar Bennani ◽  
Anne Vandendriessche ◽  
Laurence Hellouin de Menibus ◽  
Vincent Langlois ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Granule Cell ◽  
Opportunistic Infections ◽  
Jc Virus ◽  
Immunocompromised Patient ◽  
Chain Reaction ◽  
Progressive Cerebellar Ataxia ◽  
Polymerase Chain ◽  
Granule Cell Neuronopathy

Abstract Neurological opportunistic infections are going to increase. Clinicians should be aware of the neurological spectrum of JC virus manifestations, including granule cell neuronopathy. Detection of JC virus DNA by polymerase chain reaction in cerebrospinal fluid should be realized in the assessment of a progressive cerebellar ataxia in an immunocompromised patient.

Polymerase chain reaction for detection of JC virus DNA in cerebrospinal fluid: a quality control study

1997 ◽  
Vol 69 (1-2) ◽  
pp. 231-237 ◽  
Author(s):  
T. Weber ◽  
P.E. Klapper ◽  
G.M. Cleator ◽  
M. Bodemer ◽  
W. Lüke ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Quality Control ◽  
Cerebrospinal Fluid ◽  
Jc Virus ◽  
Chain Reaction ◽  
Quality Control Study ◽  
Polymerase Chain ◽  
Control Study

scholarly journals Improving detection of JC virus by ultrafiltration of cerebrospinal fluid before polymerase chain reaction for the diagnosis of progressive multifocal leukoencephalopathy

BMC Neurology ◽  
2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Kazuo Nakamichi ◽  
Michi Kawamoto ◽  
Junko Ishii ◽  
Masayuki Saijo
Keyword(s):  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Detection Limit ◽  
Progressive Multifocal Leukoencephalopathy ◽  
Real Time ◽  
Real Time Pcr ◽  
Jc Virus ◽  
Standard Procedure ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract Background Progressive multifocal leukoencephalopathy (PML) is a demyelinating disorder caused by JC virus (JCV). Although detecting JCV DNA in the cerebrospinal fluid (CSF) by real-time polymerase chain reaction (PCR) is useful, diagnosis is difficult when JCV concentrations are low. We therefore aimed to lower the detection limit of real-time PCR testing by enriching JCV in the CSF via ultrafiltration. Methods Virus suspensions and CSF specimens from 20 untreated patients with suspected PML were collected and total DNAs were extracted. The JCV large T gene was detected by quantitative real-time PCR under condition with and without prior centrifugal ultrafiltration. Results The JCV DNA was reliably detected to a lower limit of 10 copies/mL of virus suspension by real-time PCR with ultrafiltration. When using this method, the quantity of JCV DNA per PCR reaction increased 3.2- to 8.7-fold compared with the standard procedure. Seven patients were positive for JCV when using the standard procedure, and an additional patient was positive when using ultrafiltration. All JCV-positive patients had neurological features and magnetic resonance imaging findings compatible with PML. Conclusions The detection limit of JCV DNA by real-time PCR can be lowered by viral enrichment using ultrafiltration. Our simple protocol offers a valuable tool for PML diagnosis when extremely low copy numbers of JCV are released into the CSF or when brain biopsy is not feasible.

scholarly journals Longest reported case of symptomatic COVID-19 reporting positive for over 230 days in an immunocompromised patient in the United States

2021 ◽  
Vol 9 ◽  
pp. 2050313X2110400
Author(s):  
Bilal Chaudhry ◽  
Lidiya Didenko ◽  
Maaria Chaudhry ◽  
Andrew Malek ◽  
Kirill Alekseyev
Keyword(s):  
Risk Factors ◽  
Polymerase Chain Reaction ◽  
Immunocompromised Patient ◽  
The United States ◽  
Immunocompromised Patients ◽  
Chain Reaction ◽  
Viral Shedding ◽  
Time Period ◽  
Increased Risk ◽  
Polymerase Chain

Coronavirus 2019 (COVID-19) pneumonia was first noted in Wuhan, China. Since the start of the pandemic, there have been millions of cases diagnosed. The average time from onset of symptoms to testing negative SARS-CoV-2 via reverse transcription polymerase chain reaction is roughly 25 days. In patients who continually test positive for COVID-19, it is essential to determine precisely which risk factors contribute to the increase in viral shedding duration. We present a case about a 62-year-old man who has persistently tested positive for COVID-19 for more than 230 days. We followed his treatment course, in which he had been hospitalized multiple times since the onset of symptoms back in April 2020. We have determined that patients with immunosuppression, especially those taking corticosteroids, are at increased risk of prolonged viral shedding. It is essential to continually monitor these immunocompromised patients as they required a greater time period in order to have an appropriate immune response in which antibodies are created.

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