scholarly journals Subcutaneous Aspergillus nodule with cutaneous Enterococcus infection

2021 ◽  
Vol 2021 (9) ◽  
Author(s):  
Makoto Kondo ◽  
Keiichi Yamanaka
Keyword(s):  
Tissue Culture ◽  
Enterococcus Faecalis ◽  
Bacterial Culture ◽  
Subcutaneous Tissue ◽  
Skin Surface ◽  
Deep Infection ◽  
Left Forearm ◽  
Culture Form ◽  
Infectious Organism ◽  
Surface Scale

ABSTRACT An 87-year-old woman presented with a subcutaneous nodule with overlying black and yellow scales on the surface located on the left forearm. Enterococcus faecalis grew up in bacterial culture using specimen from skin surface scale. And Aspergillus fumigatus was detected in the subcutaneous tissue culture. When suspecting a deep infection, not only the surface part but also the deeper part must be cultured, because we may mislead about the identity of the infectious organism only bacterial culture form skin surface.

Microangiopathy in Severe Chronic Venous Insufficiency Evaluated by Fluorescence Video-Microscopy

1986 ◽  
Vol 1 (3) ◽  
pp. 159-169 ◽  
Author(s):  
P. Haselbach ◽  
U. Vollenweider ◽  
G. Moneta ◽  
A. Bollinger
Keyword(s):  
Intravenous Injection ◽  
Chronic Venous Insufficiency ◽  
Venous Insufficiency ◽  
Subcutaneous Tissue ◽  
Flow Distribution ◽  
Skin Surface ◽  
Video Microscopy ◽  
Healthy Controls ◽  
Microvascular Flow ◽  
Made In

Fluorescence video microscopy after intravenous injection of Na-fluorescein was used to study capillary morphology, pericapillary halo diameters, microvascular flow distribution and transcapillary diffusion of the dye in 15 healthy controls and 15 patients with severe chronic venous insufficiency (CVI). The recordings were made in the medial ankle region. Transcapillary diffusion was monitored within a densitometer window encompassing 3.2 mm2 of skin surface. Microangiopathy known from previous studies was documented in the patients with severe CVI. The number of skin capillaries within the field of observation was not reduced. In some cases inhomogeneous microvascular flow distribution and probable microthrombosis were detected. Mean halo size averaged 81 — 15 μm in the controls and 146 ± 47 μm in the patients (P < 0.001). Unexpectedly, transcapillary diffusion of Na-fluorescein was not significantly increased in the field of measurement. Possible explanations include asynchronous inflow of the dye, the presence of thrombosed and therefore not perfused capillaries, a pericapillary fibrin layer limiting diffusion and redistribution of flow in favour of the subcutaneous tissue.

scholarly journals Empyema Necessitans in the Setting of Methicillin-Susceptible Staphylococcus aureus Causing Pneumonia and Bacteremia

2018 ◽  
Vol 2018 ◽  
pp. 1-5
Author(s):  
Sindhura Bandaru ◽  
Sukesh Manthri ◽  
Vidya Sundareshan ◽  
Vidhya Prakash
Keyword(s):  
Staphylococcus Aureus ◽  
Chest Wall ◽  
Subcutaneous Tissue ◽  
Parietal Pleura ◽  
Intravenous Drug Use ◽  
Rare Occurrence ◽  
Left Forearm ◽  
Gram Stain ◽  
Incision And Drainage ◽  
Prompt Diagnosis

Empyema necessitans (EN) is a rare phenomenon that refers to an insidious extension of the empyema through parietal pleura and subsequent dissection into subcutaneous tissue of the chest wall. A 29-year-old man presented to the hospital with fever and chills a few days after an inadvertent needle stick while injecting heroin. His left forearm was warm with an area of fluctuance. He underwent incision and drainage of the left forearm abscess with fluid submitted for Gram stain and culture. His condition rapidly deteriorated due to sepsis, and he required transfer to the intensive care unit. A new 4 × 3 cm area over the left pectoralis muscle had become increasingly indurated, fluctuant, and erythematous. CT of the chest demonstrated extensive cavitary lung lesions and a large loculated left-sided pleural effusion with extension through the chest wall. TEE revealed a 3 cm complex lesion on the superior septal leaflet of the tricuspid valve. The patient underwent incision and drainage of the pectoralis major EN with placement of a drain. Blood and sputum cultures grew methicillin-susceptible Staphylococcus aureus (MSSA) at which time antibiotic therapy was tailored to oxacillin. Our case highlights a rare occurrence of EN due to MSSA in a patient with intravenous drug use (IDU) and underscores the importance of prompt diagnosis and treatment.

scholarly journals Two dimensional temperature distribution model in human dermal region exposed at low ambient temperatures with air flow

2013 ◽  
Vol 8 (2) ◽  
pp. 11-24 ◽  
Author(s):  
DB Gurung
Keyword(s):  
Subcutaneous Tissue ◽  
Air Flow ◽  
Static Condition ◽  
Skin Surface ◽  
Distribution Model ◽  
Two Dimensional ◽  
Ambient Temperatures ◽  
Insensible Perspiration ◽  
Engineering And Technology ◽  
The Mathematical Model

This paper deals with thermo-regulation in human dermal part in a cold atmosphere with significant air flow. The mathematical model involving bio-heat equation has been solved using finite element method and Crank-Nicolson technique to numerically investigate two dimensional temperature distributions. The natural three layers of dermal part – epidermis, dermis, and subcutaneous tissue are considered for the study. The important parameters like blood mass flow rate, metabolic heat generation rate and thermal conductivity are taken distinct in each layer according to their distinct sub-regional activities. The human subject is assumed in static condition. The wind speed is considered in the range from the start of forced convection (? 0.2 m/s) and up to 5 m/s. The loss of heat from the skin surface to the environment is taken due to convection, radiation, and insensible perspiration. Kathmandu University Journal of Science, Engineering and Technology Vol. 8, No. II, December, 2012, 11-24 DOI: http://dx.doi.org/10.3126/kuset.v8i2.7320

scholarly journals Thermal Field Analysis of Two Kinds of Suspended Moxibustion

2021 ◽  
Author(s):  
Bo Zhang ◽  
Shan Huang ◽  
Hanrui Zhang ◽  
Haiyan Tu
Keyword(s):  
Body Temperature ◽  
Temperature Change ◽  
Thermal Field ◽  
Subcutaneous Tissue ◽  
Skin Surface ◽  
The Body ◽  
Field Analysis ◽  
Transfer Model ◽  
Suspended Moxibustion ◽  
Mild Moxibustion

Abstract Background: Moxibustion has been widely used in Chinese medicine treatment and health care, it is necessary to study the mechanism and characteristics of moxibustion treatment. The warm effect of moxibustion is closely related to the therapeutic effect. Studying the distribution and change of thermal field is an effective way to understand the mechanism of moxibustion. Methods: The finite element analysis software COMSOL was used to establish the heat transfer model to simulate the whole moxibustion process. Two kinds of suspended moxibustion methods, including mild moxibustion and sparrow-pecking moxibustion, were used to perform moxibustion trial at Zusanli acupoint (ST36) of volunteers, with Institutional Review Board (IRB) approval, and the thermal field on the skin surface was detected with an infrared thermometer. Results: Moxibustion method and moxibustion distance are the factors that affect the temperature change. The temperature rising speed of mild moxibustion was slower than that of sparrow-pecking moxibustion. When the moxibustion distance changes, the temperature changes obviously. If the moxibustion distance does not change, the body temperature will continue to rise slowly. If the distance increases, the body temperature will show a downward trend. On the contrary, it goes up. The thermal fields of mild moxibustion and sparrow-pecking moxibustion were distributed in concentric circles around Zusanli. After natural cooling, the temperature of subcutaneous tissue was higher than that of epidermis.Conclusion: The speed of skin temperature change is affected by the method of moxibustion. The temperature field characteristics formed on the skin surface and acupoint are affected by the moxibustion distance and the moxibustion duration. Moxibustion will produce warm heat stimulation to the subcutaneous tissue.

scholarly journals Evaluasi Metode Skrining Biofilm Enterococcus faecalis Isolat Klinik Kateter Urin

2019 ◽  
Author(s):  
Wani Devita Gunardi ◽  
Mohamad Yanuar Prasetyo Nugroho ◽  
Elisabeth D. Harahap
Keyword(s):  
Tissue Culture ◽  
Enterococcus Faecalis ◽  
Congo Red ◽  
National Healthcare ◽  
National Healthcare Safety Network ◽  
Culture Plate ◽  
Tissue Culture Plate

Laporan National Healthcare Safety Network dari tahun 2006 - 2008, menunjukan penyebab paling umum kedua infeksi saluran kemih (ISK) terkait kateter adalah genus Enterococcus setelah Eschericia coli. Pada infeksi saluran kemih terkait kateter urin, faktor yang berperan penting dalam patogenesis infeksi ini, yaitu: pembentukan biofilm pada kateter urin. Peranan Enterococcus faecalis sebagai penyebab infeksi saluran kemih berkaitan dengan kemampuannya dalam membentuk biofilm. Ada beberapa variasi metode untuk mendeteksi pembentukan biofilm seperti Tissue Culture Plate (TCP), Tube method (TM), dan Congo Red Agar (CRA). Tujuan penelitian ini untuk mendapatkan metode deteksi pembentukan biofilm dari E. faecalis yang tepat, cepat, dan mudah dilakukan. Total tigabelas isolat bakteri E. faecalis yang didapat dari hasil isolasi kultur kateter urin dilakukan uji deteksi pembentukan biofilm dengan metode TCP sebagai baku emas dan CRA sebagai pemeriksaan pembanding. Hasilnya, didapatkan 61,5% dan 69,2% bakteri E. faecalis mampu menghasilkan biofilm menggunakan metode TCP dan CRA. Hasil uji diagnostik metode CRA dibandingkan dengan metode TCP untuk deteksi pembentukan biofilm, didapatkan sensitivitas dan spesifisitas dari CRA sebesar 75% dan 40%. CRA merupakan metode yang cepat dan mudah untuk dilakukan, namun memiliki spesifitas yang kurang baik. Hal ini dapat disebabkan pembacaan hasil yang bersifat subjektif dan menimbulkan kesalahan paralaks. Kata kunci : Enterococcus faecalis, Kateter Urin, Biofilm.

Microbiological Comparison of Royal Jelly and Chlorhexidine 0.2%

2017 ◽  
Vol 3 (2) ◽  
pp. 123
Author(s):  
Aida Meto ◽  
Agron Meto ◽  
Edit Xhajanka
Keyword(s):  
Enterococcus Faecalis ◽  
Royal Jelly ◽  
Bacterial Culture ◽  
Antibacterial Property ◽  
Blood Agar ◽  
Antibacterial Action ◽  
Dental Practice ◽  
Sterile Condition

The aim of this paper is to evaluate the antibacterial property of royal jelly and chlorhexidine 0.2%. As a methodology, in our study, we used piastres in blood agar, where the holes in the agar field were made through a glass pipette, sterile "Paster", in a diameter of 7 mm. Used a bacterial culture of Streptococcus gr. D (Enterococcus faecalis) in a concentration of 105, which was distributed in sterile condition, using a sterile tampon, according to the method of diffusion in agar. As a result, we used a ruler for the measurement of inhibition areas: -in the royal jelly’s hole, the radius of inhibition resulted 14 mm, -in the chlorhexidine’s hole, the radius of inhibition resulted 20 mm. Based on the results obtained from our study, presented facts to use the royal jelly and chlorhexidine 0.2% in the dental practice. As a conclusion, we can say that the royal jelly contains important elements with antibacterial action compared to the chlorhexidine one.

Survival and Function of Bioengineered Cardiac Grafts

Circulation ◽  
1999 ◽  
Vol 100 (suppl_2) ◽  
Author(s):  
Ren-Ke Li ◽  
Zhi-Qiang Jia ◽  
Richard D. Weisel ◽  
Donald A. G. Mickle ◽  
Angel Choi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Subcutaneous Tissue ◽  
Scar Tissue ◽  
Myocardial Scar ◽  
Fetal Rat ◽  
Myocardial Scar Tissue ◽  
And Function ◽  
Cardiac Graft

Introduction —Patients with congenital heart disease frequently require graft material for repair of cardiac defects. However, currently available grafts lack growth potential and are noncontractile and thrombogenic. We have developed a viable cardiac graft that contracts spontaneously in tissue culture by seeding cells derived from fetal rat ventricular muscle into a biodegradable material. We report our investigations of the in vitro and in vivo survival and function of this bioengineered cardiac graft. Methods and Results —A cardiomyocyte-enriched cell inoculum derived from fetal rat ventricular muscle was seeded into a piece of Gelfoam (Upjohn, Ontario, Canada), a biodegradable gelatin mesh, to form the graft. For in vitro studies, growth patterns of the cells within the graft were evaluated by constructing growth curves and by histologic examination; in in vivo studies, the graft was cultured for 7 days and then implanted either into the subcutaneous tissue of adult rat legs or onto myocardial scar tissue in a cryoinjured rat heart. Five weeks later, the graft was studied histologically. The inoculated cells attached to the gelatin mesh and grew in 3 dimensions in tissue culture, forming a beating cardiac graft. In both the subcutaneous tissue and the myocardial scar, blood vessels grew into the graft from the surrounding tissue. The graft implanted into the subcutaneous tissue contracted regularly and spontaneously. When implanted onto myocardial scar tissue, the cells within the graft survived and formed junctions with the recipient heart cells. Conclusions —Fetal rat ventricular cells can grow 3-dimensionally in a gelatin mesh. The cells in the graft formed cardiac tissue and survived and contracted spontaneously both in tissue culture and after subcutaneous implantation. Future versions of this bioengineered cardiac graft may eventually be used to repair cardiac defects.

Numerical Simulation of Insulin Depot Formation in Subcutaneous Tissue Comparing Different Cannula Geometries

2016 ◽  
Author(s):  
Michael M. Zedelmair ◽  
Abhijit Mukherjee
Keyword(s):  
Insulin Pump ◽  
Subcutaneous Tissue ◽  
Skin Surface ◽  
Insulin Analogues ◽  
Insulin Pump Therapy ◽  
Pump Therapy ◽  
Anisotropic Porous Media ◽  
Ansys Fluent ◽  
Sink Term ◽  
The Impact

In this study, the impact of the cannula geometry on the formation of the depot in subcutaneous tissue is investigated when injecting insulin using an insulin pump. The simulations have been conducted using the Computational Fluid Dynamics (CFD) software ANSYS Fluent. The study is focusing on rapid acting insulin analogues typically used in insulin pump therapy, which enter the bloodstream very shortly after administration. A previously developed 2-dimensional simulation has been transferred into a 3-dimensional case in order to simulate cases with non-axisymmetric geometries. The tissue has been modeled as a homogeneous anisotropic porous media by the use of different porosity values in the parallel and perpendicular direction with respect to the skin surface. The process of absorption is implemented into the model by the use of a locally variable species sink term. The basic case, simulated with a solid cannula, has been compared to other cannula geometries in order to evaluate if the delivery of insulin in the tissue can be improved. The geometries under consideration are the addition of circumferential holes in the wall of the cannula as well as using an array of cannulas instead of a single cannula. The depot formation is analyzed simulating a standard bolus injection of 0.05ml of insulin using an injection time of 25 seconds. It is observed that the addition of multiple holes in the wall of the cannula or using an array of cannulas can alter the shape of the depot quite significantly. The impact of the depot shape on the diffusion of insulin in the tissue has been evaluated by measuring the total volume of the depot after injection.

scholarly journals Physical Properties, Antimicrobial Activity and In Vivo Tissue Response to Apexit Plus

Materials ◽  
2020 ◽  
Vol 13 (5) ◽  
pp. 1171 ◽  
Author(s):  
Roberto Alameda Hoshino ◽  
Guilherme Ferreira da Silva ◽  
Mateus Machado Delfino ◽  
Juliane Maria Guerreiro-Tanomaru ◽  
Mario Tanomaru-Filho ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Physical Properties ◽  
Enterococcus Faecalis ◽  
Subcutaneous Tissue ◽  
Setting Time ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Control Group ◽  
Significant Difference

We investigated the physical properties, antimicrobial activity, and tissue reaction to Apexit Plus in comparison to Sealapex. Flow, radiopacity, setting time, and solubility were evaluated in each material. The antimicrobial activity against Enterococcus faecalis was performed. Polyethylene tubes containing Apexit Plus or Sealapex, and without material (control group) were implanted into the subcutaneous tissue of rats. At 7, 15, 30, and 60 days of implantation, the specimens were paraffin-embedded and the number of inflammatory cells (ICs) and the amount of birefringent collagen (BC) were quantified. The von Kossa reaction followed by immunohistochemistry for detection of alkaline phosphatase (ALP) was also performed. Statistical analysis was performed with ANOVA and Tukey test (p ≤ 0.05). The flow value of Apexit Plus was greater than Sealapex, whereas the radiopacity (3.44 mm Al) was lower than Sealapex (6.82 mm Al). Apexit Plus showed lower solubility and shorter initial and final setting (p < 0.0001), whereas the antimicrobial activity was significantly greater than Sealapex. Although the number of ICs was higher in Apexit Plus (p = 0.0009) at 7 days, no significant difference was detected between Apexit Plus and Sealapex at 15, 30, and 60 days. All groups showed higher values for BC in the capsules over time. ALP-immunolabelled cells were observed, mainly around von Kossa-positive structures, either in the capsules of Apexit Plus or Sealapex. Therefore, our results revealed that Apexit Plus exhibited a greater effectiveness against Enterococcus faecalis and better physical properties than Sealapex, except for the radiopacity. In vivo findings indicate that Apexit Plus is biocompatible and presents potential bioactivity in the subcutaneous tissue.

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