Subchronic and Low Dose of Tributyltin Exposure Leads to Reduced Ovarian Reserve, Reduced Uterine Gland Number, and Other Reproductive Irregularities in Female Mice

Abstract Tributyltin (TBT) chloride is an endocrine disrupting chemical associated with reproductive complications. Studies have shown that TBT targets the reproductive tract, impairing ovarian folliculogenesis, and uterine morphophysiology. In this investigation, we assessed whether subchronic and low dose of TBT exposure results in abnormal ovarian follicular reserve and other irregularities in female mice. TBT was administered to female mice (500 ng/kg/day for 12 days via gavage), and reproductive tract morphophysiology was assessed. We further assessed reproductive tract inflammation and oxidative stress. Improper functioning of the reproductive tract in TBT mice was observed. Specifically, irregular estrous cyclicity and abnormal ovarian morphology coupled with reduction in primordial and primary follicle numbers was observed, suggesting ovarian reserve depletion. In addition, improper follicular development and a reduction in antral follicles, corpora lutea, and total healthy ovarian follicles together with an increase in cystic follicles were apparent. Evidence of uterine atrophy, reduction in endometrial gland number, and inflammation and oxidative stress were seen in TBT mice. Further, strong negative correlations were observed between testosterone levels and primordial, primary, and total healthy ovarian follicles. Thus, these data suggest that the subchronic and low dose of TBT exposure impaired ovarian follicular reserve, uterine gland number, and other reproductive features in female mice.

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Low-dose irradiation reduces forced swim test-induced immobility and oxidative stress in mice

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2020.12.425 ◽

2021 ◽

Vol 165 ◽

pp. 56-57

Author(s):

Shota Naoe ◽

Takahiro Kataoka ◽

Hina Shuto ◽

Junki Yano ◽

Tetsuya Nakada ◽

...

Keyword(s):

Oxidative Stress ◽

Low Dose ◽

Forced Swim Test ◽

Swim Test ◽

Dose Irradiation ◽

Forced Swim ◽

And Oxidative Stress

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Comparative study of hydrochlorothiazide and indapamide on the anti-atherogenic potential of losartan in cholesterol fed rat

Bangladesh Medical Research Council Bulletin ◽

10.3329/bmrcb.v36i1.5447 ◽

1970 ◽

Vol 36 (1) ◽

pp. 14-19

Author(s):

Md. Zakirul Islam ◽

Md. Sayedur Rahman

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Low Dose ◽

Rat Aorta ◽

Antioxidant Effect ◽

Hypolipidemic Effect ◽

Cholesterol Diet ◽

Cholesterol Feeding ◽

Atherosclerotic Change ◽

And Oxidative Stress

The study was conducted to evaluate the anti-atherogenic potential of losartan and to assess the effects of hydrochlorothiazide and indapamide on losartan activity in rat. Cholesterol diet (0.5%) for 12 weeks led to significant hyperlipidemia, increased body weight and oxidative stress in erythrocyte. While, losartan, hydrochlorothiazide and indapamide treatment continued for next 12 weeks, losartan showed anti-atherogenic activity reflected by hypolipidemic effect and antioxidant effect in erythrocyte. This activity was abolished by addition of hydrochlorothiazide with losartan but remained unaltered by addition of indapamide with losartan. Atherosclerotic change and oxidative stress were not found in rat aorta, which may be due to short duration and low dose of cholesterol feeding. Hydrochlorothiazide treatment was associated with hypokalemia, which was not present in losartan or indapamide treatment. This study suggests that indapamide might be co-administered with losartan conserving the essential anti-atherogenic potential of losartan.Online: 13 July 2010DOI: http://dx.doi.org/10.3329/bmrcb.v36i1.5447Bangladesh Med Res Counc Bull 2010; 36: 14-19

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Morinda Citrifolia (Noni) and Low Dose Aspirin Prevent Apoptotic Cell Death and Oxidative Stress on Isoproterenol Induced Myocardial Infarction in Rats

Erciyes Medical Journal ◽

10.5152/etd.2017.1732 ◽

2017 ◽

Vol 39 (4) ◽

pp. 165-170 ◽

Cited By ~ 3

Author(s):

Kevser Kusat Ol ◽

Gungor Kanbak ◽

Aysegul Oglakci Ilhan ◽

Kazim Kartkaya ◽

Mine Erden Inal

Keyword(s):

Oxidative Stress ◽

Myocardial Infarction ◽

Cell Death ◽

Low Dose ◽

Apoptotic Cell ◽

Apoptotic Cell Death ◽

Morinda Citrifolia ◽

Dose Aspirin ◽

Low Dose Aspirin ◽

And Oxidative Stress

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Metformin Corrects Glucose Metabolism Reprogramming and NLRP3 Inflammasome-Induced Pyroptosis via Inhibiting the TLR4/NF-κB/PFKFB3 Signaling in Trophoblasts: Implication for a Potential Therapy of Preeclampsia

Oxidative Medicine and Cellular Longevity ◽

10.1155/2021/1806344 ◽

2021 ◽

Vol 2021 ◽

pp. 1-22

Author(s):

Yang Zhang ◽

Weifang Liu ◽

Yanqi Zhong ◽

Qi Li ◽

Mengying Wu ◽

...

Keyword(s):

Oxidative Stress ◽

Nlrp3 Inflammasome ◽

Low Dose ◽

Therapeutic Potential ◽

Metabolic Phenotypes ◽

Pyrin Domain ◽

Underlying Mechanisms ◽

And Oxidative Stress ◽

Receptor Family

NOD-like receptor family, pyrin domain-containing protein 3 (NLRP3) inflammasome-mediated pyroptosis is a crucial event in the preeclamptic pathogenesis, tightly linked with the uteroplacental TLR4/NF-κB signaling. Trophoblastic glycometabolism reprogramming has now been noticed in the preeclampsia pathogenesis, plausibly modulated by the TLR4/NF-κB signaling as well. Intriguingly, cellular pyroptosis and metabolic phenotypes may be inextricably linked and interacted. Metformin (MET), a widely accepted NF-κB signaling inhibitor, may have therapeutic potential in preeclampsia while the underlying mechanisms remain unclear. Herein, we investigated the role of MET on trophoblastic pyroptosis and its relevant metabolism reprogramming. The safety of pharmacologic MET concentration to trophoblasts was verified at first, which had no adverse effects on trophoblastic viability. Pharmacological MET concentration suppressed NLRP3 inflammasome-induced pyroptosis partly through inhibiting the TLR4/NF-κB signaling in preeclamptic trophoblast models induced via low-dose lipopolysaccharide. Besides, MET corrected the glycometabolic reprogramming and oxidative stress partly via suppressing the TLR4/NF-κB signaling and blocking transcription factor NF-κB1 binding on the promoter PFKFB3, a potent glycolytic accelerator. Furthermore, PFKFB3 can also enhance the NF-κB signaling, reduce NLRP3 ubiquitination, and aggravate pyroptosis. However, MET suppressed pyroptosis partly via inhibiting PFKFB3 as well. These results provided that the TLR4/NF-κB/PFKFB3 pathway may be a novel link between metabolism reprogramming and NLRP3 inflammasome-induced pyroptosis in trophoblasts. Further, MET alleviates the NLRP3 inflammasome-induced pyroptosis, which partly relies on the regulation of TLR4/NF-κB/PFKFB3-dependent glycometabolism reprogramming and redox disorders. Hence, our results provide novel insights into the pathogenesis of preeclampsia and propose MET as a potential therapy.

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Follicular development, morphological integrity, and oxidative stress in bovine preantral follicles cultured in vitro with ascorbic acid

Zygote ◽

10.1017/s0967199421000903 ◽

2021 ◽

pp. 1-7

Author(s):

Larissa Zamparone Bergamo ◽

Denis Vinicius Bonato ◽

Camila Bizarro-Silva ◽

Francieli Gesleine Capote Bonato ◽

Tamires Korchovei Sanches ◽

...

Keyword(s):

Oxidative Stress ◽

Ascorbic Acid ◽

Culture Medium ◽

Bos Taurus ◽

Follicular Development ◽

Ovarian Follicles ◽

Bos Taurus Indicus ◽

Bonferroni Test ◽

And Oxidative Stress

Summary The aim of this study was to evaluate the follicular development, morphological integrity, and oxidative stress of preantral ovarian follicles from Bos taurus indicus females grown in vitro with ascorbic acid. Ovaries (n = 20) from Bos taurus indicus females were collected, fragmented, and were cultured in vitro for 6 or 12 days in minimum essential medium (MEM), or MEM supplemented with 50 or 100 ng/ml ascorbic acid, with an extracellular matrix of agarose gel, in an incubator at 38.5°C; every 2 days, 100% of the culture medium was replaced. The data were analyzed using the chi-squared test and/or Fisher’s exact test. In the event of a significant effect, the proportions were compared using a 2 × 2 proportion test. The oxidative stress analysis data were submitted to analysis of variance followed by the Bonferroni test. Values were considered significant when P ≤ 0.05. The addition of 100 ng/ml of ascorbic acid to the in vitro culture medium of preantral ovarian follicles from bovine females promoted follicular development, was efficient in maintaining morphological integrity, as well as the stability of reactive oxygen species, after 6 days of in vitro culture.

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Effect of low-dose chronic gamma exposure on growth and oxidative stress related responses inArabidopsis thaliana

Radioprotection ◽

10.1051/radiopro/20095090 ◽

2009 ◽

Vol 44 (5) ◽

pp. 487-491 ◽

Cited By ~ 5

Author(s):

H. Vandenhove ◽

N. Vanhoudt ◽

J. Wannijn ◽

M. Van Hees ◽

A. Cuypers

Keyword(s):

Oxidative Stress ◽

Low Dose ◽

And Oxidative Stress

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EFFECT OF CIMETIDINE ON NITRO-OXIDATIVE STRESS IN A RAT MODEL OF PERIODONTITIS

Medicine and Pharmacy Reports ◽

10.15386/cjmed-273 ◽

2014 ◽

Vol 87 (3) ◽

pp. 177-181 ◽

Cited By ~ 2

Author(s):

Carina Culic ◽

Alina Elena Parvu ◽

Sandu Florin Alb ◽

Camelia Alb ◽

Angela Pop

Keyword(s):

Oxidative Stress ◽

Methyl Ester ◽

Rat Model ◽

Low Dose ◽

Oxidative Status ◽

Stress Index ◽

Oxidative Stress Index ◽

Total Antioxidant ◽

Total Oxidative Status ◽

And Oxidative Stress

Background and aims. Periodontitis is a chronic inflammation that involves nitro-oxidative stress with damaging periodontal structural effects. We aimed to evaluate the consequences of low-dose cimetidine on nitro-oxidative stress in periodontitis. Methods. A rat model of ligature-induced periodontitis was used. After two weeks, the periodontitis groups were treated with cimetidine, aminoguanidine, N-nitro-L-arginine methyl ester and trolox for one week. On day 21, blood was drawn and the serum analyzed for measurement of total nitrites and nitrates, total oxidative status, total antioxidant response, and oxidative stress index. Results. Cimetidine had an inhibitory effect on the synthesis of nitric oxide (p=0.001), total oxidative status (p=0.01) and oxidative stress index (p=0.01). Total antioxidant reactivity was increased by cimetidine (p=0.01). The effects of cimetidine were almost like those of aminoguanidine, NG-nitro-L-arginine methyl ester, and trolox. Conclusions. Low-dose cimetidine can be used as adjunctive host modulatory therapy in chronic periodontitis because it reduces nitro-oxidative stress.

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