Mobile digital x-ray and portable cartridge based nucleic acid amplification test machines for point-of-care diagnosis of TB in rural India

2020 ◽  
Author(s):  
Bornali Datta ◽  
David Ford ◽  
Pinky Goyal ◽  
Ashish Prakash ◽  
Jaya Prasad Tripathy ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Nucleic Acid Amplification Test ◽  
Clinical Suspicion ◽  
Nucleic Acid Amplification ◽  
Rural India ◽  
X Rays ◽  
X Ray ◽  
Microbiological Confirmation ◽  
Good Success Rate

Abstract Background There is no experience of point-of-care (POC) microbiological confirmation for TB in India in field settings. Methods Under the TB-Free Haryana project, a mobile van-mounted digital x-ray and portable GeneXpert system screened all presumptive TB patients with strong clinic-radiological suspicion for TB. Results Of 1673 x-rays, 215 (13%) had findings suggestive of TB, 109 had strong clinical suspicion and were eligible for POC GeneXpert, in whom a test was performed in 82 (75%) cases; 59 (72%) tested positive and were initiated on treatment within 24 h. Conclusions A mobile van equipped with digital x-ray and POC GeneXpert is feasible and has a good success rate with potential for replication.

scholarly journals Evaluation of the Visby Medical COVID-19 Point of Care Nucleic Acid Amplification Test

2021 ◽  
Author(s):  
Brooke M. Katzman ◽  
Amy M. Wockenfus ◽  
Brandon R. Kelley ◽  
Brad S. Karon ◽  
Leslie J. Donato
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification

scholarly journals Coinfection of SARS-CoV-2 and MTB: how not to miss the wood for the trees

2021 ◽  
Vol 14 (7) ◽  
pp. e240581
Author(s):  
Jeny Jacob ◽  
Rajesh Venkataram ◽  
Giridhar Belur Hosmane
Keyword(s):  
Multiple Sclerosis ◽  
Mycobacterium Tuberculosis ◽  
Nucleic Acid ◽  
Young Woman ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Intensive Phase ◽  
X Ray ◽  
Chest X Ray ◽  
Scarce Data

Scarce data exist about the coinfection of SARS‐CoV‐2 and Mycobacterium tuberculosis (MTB). A young woman who was undergoing treatment for multiple sclerosis was brought to our hospital with a COVID-19 positive status. On further evaluation, her chest X-ray showed right upper and mid-zone opacity, which lead to the suspicion of MTB. Her sputum came positive for acid-fast bacilli (AFB) staining and cartridge-based nucleic acid amplification test (CBNAAT) confirmed it, and rifampicin resistance was not detected. She was started on an antitubercular regimen. She was discharged, and by the end of the intensive phase of treatment, her symptoms subsided, but her sputum CBNAAT still showed the presence of TB bacillus.

scholarly journals An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hao Deng ◽  
Asanka Jayawardena ◽  
Jianxiong Chan ◽  
Sher Maine Tan ◽  
Tuncay Alan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Clinical Decision Making ◽  
Point Of Care ◽  
Lamp Assay ◽  
Clinical Decision ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Negative Results ◽  
Skilled Personnel ◽  
High Level

AbstractThere is currently a high level of demand for rapid COVID-19 tests, that can detect the onset of the disease at point of care settings. We have developed an ultra-portable, self-contained, point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection, based on the principle of loop mediated isothermal amplification (LAMP). The LAMP assay is 100% sensitive and specific to detect a minimum of 300 RNA copies/reaction of SARS-CoV-2. All of the required sample transportation, lysing and amplification steps are performed in a standalone disposable cartridge, which is controlled by a battery operated, pocket size (6x9x4cm3) unit. The test is easy to operate and does not require skilled personnel. The total time from sample to answer is approximately 35 min; a colorimetric readout indicates positive or negative results. This portable diagnostic platform has significant potential for rapid and effective testing in community settings. This will accelerate clinical decision making, in terms of effective triage and timely therapeutic and infection control interventions.

scholarly journals An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection

2021 ◽  
Author(s):  
Hao Deng ◽  
Asanka Jayawardena ◽  
Jianxiong Chan ◽  
Sher Maine Tan ◽  
Tuncay Alan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Point Of Care ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification

Abstract The authors have requested that this preprint be removed from Research Square.

scholarly journals Multicenter Clinical Evaluation of the Novel Alere i Strep A Isothermal Nucleic Acid Amplification Test

2015 ◽  
Vol 53 (7) ◽  
pp. 2258-2261 ◽  
Author(s):  
Daniel M. Cohen ◽  
Michael E. Russo ◽  
Preeti Jaggi ◽  
Jennifer Kline ◽  
William Gluckman ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Clinical Laboratory ◽  
Point Of Care ◽  
Prospective Trial ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Isothermal Nucleic Acid Amplification ◽  
Negative Results ◽  
Group A ◽  
Low Sensitivity

Rapid detection of group A beta-hemolytic streptococcus (GAS) is used routinely to help diagnose and treat pharyngitis. However, available rapid antigen detection tests for GAS have relatively low sensitivity, and backup testing is recommended in children. Newer assays are more sensitive yet require excessive time for practical point-of-care use as well as laboratory personnel. The Alere i strep A test is an isothermal nucleic acid amplification test designed to offer highly sensitive results at the point of care within 8 min when performed by nonlaboratory personnel. The performance of the Alere i strep A test was evaluated in a multicenter prospective trial in a Clinical Laboratory Improvement Amendments (CLIA)-waived setting in comparison to bacterial culture in 481 children and adults. Compared to culture, the Aleri i strep A test had 96.0% sensitivity and 94.6% specificity. Discrepant results were adjudicated by PCR and found the Alere i strep A test to have 98.7% sensitivity and 98.5% specificity. Overall, the Alere i strep A test could provide a one-step, rapid, point-of-care testing method for GAS pharyngitis and obviate backup testing on negative results.

scholarly journals A Retrospective Cross-Sectional Study of the Utility of Cartridge-Based Nucleic Acid Amplification Test in Diagnosis of Pulmonary and Extrapulmonary Tuberculosis in People Living with HIV in Second Highest HIV Prevalent State in India

2021 ◽  
Author(s):  
Tade Bagbi ◽  
Ningthoukhongjam Reema ◽  
S. Bhagyabati Devi ◽  
Thangjam Gautam Singh ◽  
Mohammad Jaleel ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Cross Sectional Study ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Rifampicin Resistance ◽  
Sputum Smear ◽  
Smear Microscopy ◽  
Sectional Study ◽  
Cross Sectional ◽  
Smear Negative

Abstract Introduction Tuberculosis (TB) in people living with human immunodeficiency virus (PLHIV) is difficult to diagnose due to fewer organisms in sputum and extrapulmonary samples. Sputum culture takes 4 to 8 weeks for growth of the mycobacteria. Delayed treatment for TB in PLHIV leads to increased mortality. This study evaluated cartridge-based nucleic acid amplification test (CBNAAT) as a diagnostic tool for diagnosis of pulmonary TB (PTB) and extrapulmonary TB (EPTB) in PLHIV in the second most HIV prevalent state in India and for comparing its efficacy between Ziehl–Neelsen (ZN) staining sputum smear–positive and sputum smear–negative TB. Methods This cross-sectional study was conducted in RIMS, Imphal, with 167 PLHIV patients, age 15 years or older, having signs and symptoms of TB. Appropriate samples for sputum microscopy and CBNAAT were sent. Conclusion The overall sensitivity of sputum smear for acid-fast bacillus (AFB) was found to be 30.71% and that of CBNAAT was 38.57%. Sensitivity of CBNAAT for sputum smear–positive and sputum smear–negative TB was 100 and 11.3%, respectively. Sensitivity of ZN smear for AFB of EPTB sample was 48.1% and that of CBNAAT was 59.25%. In both PTB and EPTB, CBNAAT showed an increase in diagnosis of microbiologically confirmed PTB cases by 7.8 and 11.1%, respectively, over and above the cases diagnosed by ZN smear microscopy. Rifampicin resistance was detected in five patients. We conclude that CBNAAT is a rapid test with better sensitivity in diagnosis of PTB and EPTB in PLHIV, compared with ZN smear microscopy. It detects rifampicin resistance for multidrug-resistant TB and helps in early treatment intervention.

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