Influence of Protein Content on Durum Wheat Gluten Strength Determined by the SDS Sedimentation Test and by Other Methods

Author(s):  
Cubadda
1999 ◽  
Vol 76 (4) ◽  
pp. 582-586 ◽  
Author(s):  
N. P. Ames ◽  
J. M. Clarke ◽  
B. A. Marchylo ◽  
J. E. Dexter ◽  
S. M. Woods

2004 ◽  
Vol 84 (4) ◽  
pp. 1001-1013
Author(s):  
J. E. Dexter ◽  
M. A. Doust ◽  
C. N. Raciti ◽  
G. M. Lombardo ◽  
F. R. Clarke ◽  
...  

Since the 1980s, there have been general trends in the durum wheat milling industry to higher semolina extraction rate, and in the pasta processing industry to the use of higher drying temperatures. During this time, specification of gluten strength by gluten index, mixograph mixing properties and alveograph parameters has also become widespread. These trends prompted this study of the appropriateness of protocols for quality testing of Canadian durum wheat breeding lines. Four cultivars with intrinsic differences in yellow pigment levels and gluten strength were grown in field plots in Swift Current, Saskatchewan for three consecutive years. A laboratory-scale milling procedure was modified to produce semolina at extraction rates from about 65% to about 80%. Milling to extraction rates above 65%, the extraction rate used routinely in quality testing of Canadian durum wheat breeding lines, had a major impact on semolina ash content and colour, but did not offer any advantage in ranking cultivars for either semolina yield or semolina refinement. Gluten strength, as measured by gluten index, was independent of semolina extraction rate. Dough strength, as measured by mixograph properties and alveograph properties, showed a tendency to weakening at high extraction, particularly for strong cultivars. Semolina was processed into spaghetti using low-temperature (LT), high-temperature (HT) and ultra-high-temperature (UHT) drying cycles. The firmness of cooked spaghetti was predominantly influenced by protein content. As a result, cultivars generally ranked in spaghetti firmness according to protein content. Regardless of drying cycle or cultivar, spaghetti firmness increased as drying temperature increased. Spaghetti dried at LT was less yellow than spaghetti dried at HT or UHT, probably due to thermal inactivation of the bleaching enzyme lipoxygenase at HT and UHT. Regardless of drying cycle, spaghetti became duller, more red and less yellow as extraction rate increased. For each spaghetti trait, cultivar ranking remained relatively constant regardless of extraction rate or drying temperature. On the basis of these results, there appears to be no advantage to increasing semolina extraction rate beyond 65% for evaluation of durum wheat milling performance, gluten strength or pasta properties. In addition, it appears that one drying cycle is adequate to reliably evaluate durum wheat lines for spaghetti colour and firmness. Key words: Durum wheat (Triticum turgidum L. var durum), milling, semolina, pasta, quality screening, gluten strength, colour, texture


2020 ◽  
Vol 68 (20) ◽  
pp. 5521-5528
Author(s):  
Jorge Araya-Flores ◽  
Carlos Guzmán ◽  
Iván Matus ◽  
Roberto Parada ◽  
Gabriela Jarpa ◽  
...  

2003 ◽  
Vol 83 (3) ◽  
pp. 525-532 ◽  
Author(s):  
N. P. Ames ◽  
J. M. Clarke ◽  
B. A. Marchylo ◽  
J. E. Dexter ◽  
L. M. Schlichting ◽  
...  

Durum wheat (Triticum durum L.) cultivars with extra-strong gluten characteristics have become a unique quality type preferred by several domestic and international pasta processors. Durum genotypes with extra-strong gluten characteristics were compared to conventional gluten strength types to determine the relative levels of gluten strength and the importance of this characteristic in pasta quality. All extra-strong genotypes had similar gluten index levels that were significantly greater than cultivars traditionally grown in western Canada. For improved endproduct texture, the level of gluten strength appeared to be less important than protein content and wet gluten weight, which were the variables most closely linked to spaghetti viscoelasticity. The general positive relationships between texture and protein content of high temperature dried spaghetti followed the findings of previous studies. However, extra-strong gluten genotypes with lower protein content could not match the superior texture characteristics obtained with higher protein conventional strength cultivars. When dried at 70°C, pasta texture, measured as viscoelastic properties, was dependent on gluten strength rather than protein content, so the higher gluten strength types had an advantage even at lower protein levels. While the relationship between gluten strength and texture of low temperature dried products has been noted previously, this study shows that this relationship is somewhat dependent on having a range of gluten strength types present. Although there was a positive relationship between spaghetti viscoelasticity and protein content, and between pasta disc viscoelasticity and gluten strength, there appeared to be a negative relationship between gluten strength and protein quantity factors. Scatter plot matrices of the means indicated the negative correlation was likely due to clustering of similar genotypes, but that a positive relationship was apparent within the extra-strong gluten types. Key words: Wheat (durum), Triticum durum, pasta quality, extra-strong gluten


Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2845
Author(s):  
Pablo F. Roncallo ◽  
Carlos Guzmán ◽  
Adelina O. Larsen ◽  
Ana L. Achilli ◽  
Susanne Dreisigacker ◽  
...  

Durum wheat grains (Triticum turgidum L. ssp. durum) are the main source for the production of pasta, bread and a variety of products consumed worldwide. The quality of pasta is mainly defined by the rheological properties of gluten, an elastic network in wheat endosperms formed of gliadins and glutenins. In this study, the allelic variation at five glutenin loci was analysed in 196 durum wheat genotypes. Two loci (Glu-A1 and Glu-B1), encoding for high-molecular-weight glutenin subunits (HMW-GS), and three loci (Glu-B2, Glu-A3 and Glu-B3), encoding for low molecular weight glutenin subunits (LMW-GS), were assessed by SDS-PAGE. The SDS-sedimentation test was used and the grain protein content was evaluated. A total of 32 glutenin subunits and 41 glutenin haplotypes were identified. Four novel alleles were detected. Fifteen haplotypes represented 85.7% of glutenin loci variability. Some haplotypes carrying the 7 + 15 and 7 + 22 banding patterns at Glu-B1 showed a high gluten strength similar to those that carried the 7 + 8 or 6 + 8 alleles. A decreasing trend in grain protein content was observed over the last 85 years. Allelic frequencies at the three main loci (Glu-B1, Glu-A3 and Glu-B3) changed over the 1915–2020 period. Gluten strength increased from 1970 to 2020 coinciding with the allelic changes observed. These results offer valuable information for glutenin haplotype-based selection for use in breeding programs.


1980 ◽  
Vol 60 (1) ◽  
pp. 25-29 ◽  
Author(s):  
J. E. DEXTER ◽  
R. R. MATSUO ◽  
F. G. KOSMOLAK ◽  
D. LEISLE ◽  
B. A. MARCHYLO

The sodium dodecyl sulfate (SDS) sedimentation test was assessed as a screening procedure for durum wheat gluten strength. Thirty durum wheat lines possessing a wide range in gluten strength and spaghetti cooking quality were characterized for wheat protein, SDS-sedimentation value, micromixograph development time, and spaghetti cooking quality at optimum time and after overcooking for 10 min. A statistical analysis revealed that mixograph development time and the SDS-sedimentation test were both able to account satisfactorily for variations in gluten strength. However, SDS-sedimentation test was able to differentiate over a narrower range. The SDS-sedimentation test and wheat protein together accounted for over 40% of the variability in cooking quality at opimum time and after overcooking. These data suggest that screening tests based on the SDS-sedimentation test and wheat protein would be adequate for comparing durum wheats for gluten strength and spaghetti cooking quality.


Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2283
Author(s):  
Noureddine El Haddad ◽  
Miguel Sanchez-Garcia ◽  
Andrea Visioni ◽  
Abderrazek Jilal ◽  
Rola El Amil ◽  
...  

Crop wild relatives (CWR) are a good source of useful alleles for climate change adaptation. Here, 19 durum wheat, 24 barley, and 24 lentil elites incorporating CWR in their pedigrees were yield tested against commercial checks across 19 environments located in Morocco, Ethiopia, Lebanon, and Senegal. For each crop, the combined analysis of variance showed that genotype (G), environment (E), and genotype x environment (GxE) effects were significant for most of the traits. A selection index combining yield potential (G) and yield stability (GxE) was used to identify six CWR-derived elites for each crop matching or superior to the best check. A regression analysis using a climate matrix revealed that grain yield was mostly influenced by the maximum daily temperature and soil moisture level during the growing stages. These climatic factors were used to define five clusters (i.e., E1 to E5) of mega-environments. The CWR-derived elites significantly outperformed the checks in E1, E2, and E4 for durum wheat, and in E2 for both barley and lentil. The germplasm was also assessed for several food transformation characteristics. For durum wheat, one accession (Zeina) originating from T. araraticum was significantly superior in mixograph score to the best check, and three accessions originating from T. araraticum and T. urartu were superior for Zn concentration. For barley, 21 accessions originating from H. spontaneum were superior to the checks for protein content, six for Zn content, and eight for β-glucan. For lentil, ten accessions originating from Lens orientalis were superior to the check for protein content, five for Zn, and ten for Fe concentration. Hence, the results presented here strongly support the use of CWR in breeding programs of these three dryland crops, both for adaptation to climatic stresses and for value addition for food transformation.


Author(s):  
V. Natoli ◽  
P. Malchikov ◽  
P. De Vita ◽  
S. Shevchenko ◽  
S. Dolaberidze

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