scholarly journals Production of agrocinopine A by Ipomoea batatas agrocinopine synthase in transgenic tobacco and its effect on the rhizosphere microbial community

2021 ◽  
Author(s):  
Aiko Tanaka ◽  
Maarten Ryder ◽  
Takamasa Suzuki ◽  
Kazuma Uesaka ◽  
Nobuo Yamaguchi ◽  
...  
Keyword(s):  
Microbial Community ◽  
Sweet Potato ◽  
Dna Sequences ◽  
Crown Gall ◽  
Ipomoea Batatas ◽  
Bacterial Pathogen ◽  
Paper Electrophoresis ◽  
Biologically Active ◽  
Wide Range ◽  
Rhizosphere Microbial Community

Agrobacterium tumefaciens is a bacterial pathogen that causes crown gall disease on a wide range of eudicot plants by genetic transformation. Besides T-DNA integrated by natural transformation in vegetative tissues of plants by pathogenic Agrobacterium, previous reports have indicated that T-DNA sequences originating from ancestral Agrobacterium sp. are present in the genomes of all cultivated sweet potato (Ipomoea batatas) analyzed. Expression of Agrobacterium-derived agrocinopine synthase (ACS) gene was detected in leaf and root tissues of sweet potato, suggesting that the plant can produce agrocinopine, a sugar-phosphodiester opine considered to be utilized by Agrobacterium in crown gall. To validate the product synthesized by I. batatas ACS (IbACS), we introduced IbACS into tobacco under a constitutive promoter. High voltage paper electrophoresis followed by alkaline silver nitrate staining detected the production of an agrocinopine-like substance in IbACS1-expressing tobacco, and further MS and NMR analyses of the product confirmed that IbACS can produce agrocinopine A from natural plant substrates. The partially purified compound was biologically active in an agrocinopine A bioassay. 16S rRNA amplicon sequencing and meta-transcriptome analysis revealed that the rhizosphere microbial community of tobacco was affected by the expression of IbACS. A new species of Leifsonia (actinobacteria) was isolated as an enriched bacterium in the rhizosphere of IbACS1-expressing tobacco. This Leifsonia sp. can catabolize agrocinopine A produced in tobacco, indicating that the production of agrocinopine A attracts rhizosphere bacteria which can utilize this sugar-phosphodiester. These results suggest a potential role of IbACS conserved among sweet potato cultivars in manipulating their microbial community.

scholarly journals Detection of Viruses in Sweetpotato from Honduras and Guatemala Augmented by Deep-Sequencing of Small-RNAs

Plant Disease ◽  
2012 ◽  
Vol 96 (10) ◽  
pp. 1430-1437 ◽  
Author(s):  
M. Kashif ◽  
S. Pietilä ◽  
K. Artola ◽  
R. A. C. Jones ◽  
A. K. Tugume ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Virus Strain ◽  
Deep Sequencing ◽  
Ipomoea Batatas ◽  
Plant Viruses ◽  
Virus Infections ◽  
Potato Leaf ◽  
Wide Range ◽  
Virus C ◽  
Small Rna Deep Sequencing

Sweetpotato (Ipomoea batatas) plants become infected with over 30 RNA or DNA viruses in different parts of the world but little is known about viruses infecting sweetpotato crops in Central America, the center of sweetpotato domestication. Small-RNA deep-sequencing (SRDS) analysis was used to detect viruses in sweetpotato in Honduras and Guatemala, which detected Sweet potato feathery mottle virus strain RC and Sweet potato virus C (Potyvirus spp.), Sweet potato chlorotic stunt virus strain WA (SPCSV-WA; Crinivirus sp.), Sweet potato leaf curl Georgia virus (Begomovirus sp.), and Sweet potato pakakuy virus strain B (synonym: Sweet potato badnavirus B). Results were confirmed by polymerase chain reaction and sequencing of the amplicons. Four viruses were detected in a sweetpotato sample from the Galapagos Islands. Serological assays available to two of the five viruses gave results consistent with those obtained by SRDS, and were negative for six additional sweetpotato viruses tested. Plants coinfected with SPCSV-WA and one to two other viruses displayed severe foliar symptoms of epinasty and leaf malformation, purpling, vein banding, or chlorosis. The results suggest that SRDS is suitable for use as a universal, robust, and reliable method for detection of plant viruses, and especially useful for determining virus infections in crops infected with a wide range of unrelated viruses.

Response of the microbial community associated with sweet potato (Ipomoea batatas) to Bacillus safensis and Bacillus velezensis strains

2018 ◽  
Vol 112 (4) ◽  
pp. 501-512 ◽  
Author(s):  
Jackeline Rossetti Mateus ◽  
Joana Montezano Marques ◽  
Isabella Dal’Rio ◽  
Renata Estebanez Vollú ◽  
Marcia Reed Rodrigues Coelho ◽  
...  
Keyword(s):  
Microbial Community ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Bacillus Velezensis ◽  
Bacillus Safensis

scholarly journals The genome of cultivated sweet potato containsAgrobacteriumT-DNAs with expressed genes: An example of a naturally transgenic food crop

2015 ◽  
Vol 112 (18) ◽  
pp. 5844-5849 ◽  
Author(s):  
Tina Kyndt ◽  
Dora Quispe ◽  
Hong Zhai ◽  
Robert Jarret ◽  
Marc Ghislain ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Dna Sequences ◽  
Ipomoea Batatas ◽  
Pathogenic Bacteria ◽  
Arable Land ◽  
Artificial Chromosome ◽  
Plant Genome ◽  
Small Interfering Rnas ◽  
Food Crop ◽  
Transgenic Food

Agrobacterium rhizogenesandAgrobacterium tumefaciensare plant pathogenic bacteria capable of transferring DNA fragments [transfer DNA (T-DNA)] bearing functional genes into the host plant genome. This naturally occurring mechanism has been adapted by plant biotechnologists to develop genetically modified crops that today are grown on more than 10% of the world’s arable land, although their use can result in considerable controversy. While assembling small interfering RNAs, or siRNAs, of sweet potato plants for metagenomic analysis, sequences homologous to T-DNA sequences fromAgrobacteriumspp. were discovered. Simple and quantitative PCR, Southern blotting, genome walking, and bacterial artificial chromosome library screening and sequencing unambiguously demonstrated that two different T-DNA regions (IbT-DNA1 andIbT-DNA2) are present in the cultivated sweet potato (Ipomoea batatas[L.] Lam.) genome and that these foreign genes are expressed at detectable levels in different tissues of the sweet potato plant.IbT-DNA1 was found to contain four open reading frames (ORFs) homologous to the tryptophan-2-monooxygenase (iaaM), indole-3-acetamide hydrolase (iaaH), C-protein (C-prot), and agrocinopine synthase (Acs) genes ofAgrobacteriumspp.IbT-DNA1 was detected in all 291 cultigens examined, but not in close wild relatives.IbT-DNA2 contained at least five ORFs with significant homology to theORF14,ORF17n, rooting locus (Rol)B/RolC,ORF13, andORF18/ORF17ngenes ofA. rhizogenes.IbT-DNA2 was detected in 45 of 217 genotypes that included both cultivated and wild species. Our finding, that sweet potato is naturally transgenic while being a widely and traditionally consumed food crop, could affect the current consumer distrust of the safety of transgenic food crops.

Sweet Potato: A Potential Industrial and Staple Food Crop of Nigeria

1987 ◽  
Vol 16 (4) ◽  
pp. 178-181 ◽  
Author(s):  
S. C. O. Nwinyi
Keyword(s):  
Sweet Potato ◽  
World Economy ◽  
Ipomoea Batatas ◽  
Management Practices ◽  
Staple Food ◽  
Food Crop ◽  
Growing Period ◽  
Ecological Flexibility ◽  
Wide Range ◽  
The Impact

This article reviews the impact of sweet potato (Ipomoea batatas (L) Lam) on the world economy, its uses, and efforts in Nigeria to popularize its cultivation and acceptability. The potential and average yields of 87 and 20–30 t/ha respectively (over a three–six month growing period) under improved management practices, its ecological flexibility, its low input requirements, the wide range of recipes based on its flour, and easy propagation by vine cuttings distinguish the sweet potato as a potentially important industrial and staple food crop. Problems relating to sweetness, dominant flavour, and the colour of the cooked product are identified as ones requiring solution if its acceptability is to be enhanced.

Allelopathic Effects of Sweet Potatoes (Ipomoea batatas) on Yellow Nutsedge (Cyperus esculentus) and Alfalfa (Medicago sativa)

Weed Science ◽  
1986 ◽  
Vol 34 (4) ◽  
pp. 623-627 ◽  
Author(s):  
Howard F. Harrison ◽  
Joseph K. Peterson
Keyword(s):  
Root Growth ◽  
Medicago Sativa ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Cyperus Esculentus ◽  
Sweet Potatoes ◽  
Allelopathic Potential ◽  
Yellow Nutsedge ◽  
Potting Medium ◽  
Wide Range

Greenhouse and laboratory studies were conducted to determine the allelopathic potential of two sweet potato [Ipomoea batatasL. (Lam.)] cultivars, ‘Regal’ and ‘SC 1149-19’. Yellow nutsedge (Cyperus esculentusL. # CYPES) and alfalfa (Medicago sativaL.) plants grown in soil from sweet potato field plots accumulated less dry matter than plants grown in soil from adjacent weedy plots. Growth of yellow nutsedge and alfalfa plants was also reduced when grown in a potting medium containing decomposing sweet potato plants in comparison to plants grown in potting medium alone. When the sweet potato potting medium mixture was incubated at 25 C and tested weekly using an alfalfa growth bioassay, inhibition was high initially but decreased over time and was not observed after a 12-week incubation. Aqueous methanol (50%)-soluble extracts of sweet potato periderm were inhibitory to yellow nutsedge root growth in vermiculite and alfalfa seed germination on filter paper. Regal extracts were inhibitory to yellow nutsedge root growth at a concentration of 2.5 mg periderm extracted/ml, but SC 1149-19 extract was inhibitory only at a concentration eight times higher. Similar differences between cultivars were observed with the alfalfa germination bioassay. Preliminary separation of the Regal periderm extract by paper chromatography indicated the presence of phenolic compounds with a wide range of polarities. Several of these UV-visible compounds were inhibitory to alfalfa germination.

scholarly journals EVOLUTIONARY BIOLOGY OF THE SWEET POTATO AND ITS RELATIVES: OPPORTUNITIES FOR MOLECULAR GENETICSTUDIES

HortScience ◽  
1991 ◽  
Vol 26 (5) ◽  
pp. 492e-492
Author(s):  
G. S. Varadarajan ◽  
C. S. Prakash
Keyword(s):  
Sweet Potato ◽  
Dna Sequences ◽  
Evolutionary Biology ◽  
Ipomoea Batatas ◽  
Chromosome Doubling ◽  
Molecular Genetic ◽  
Entire Group ◽  
Dna Variation ◽  
Potential Applications ◽  
History Of

The sweet potato (Ipomoea batatas) and its relatives (the batatas complex) appear to have evolved in the New World and radiated over several geographic centers in the tropics and subtropics. Traditional studies on taxonomy, cytogenetics, and reproductive biology of the batatas complex have enabled us to investigate certain evolutionary aspects. We conclude that this complex is a monophytetic, “polyploid pillar”, evolved by chromosome doubling (euploidy) and interspecific hybridization. We apply molecular genetic techniques to detect variation [restriction fragment length polymorphism (RFLP) and DNA fingerprinting analyses] to reexamine some of the evolutionary issues that could not be satisfactorily addressed by the conventional approaches, e. g., phylogenetic history of the batatas group, the diploid ancestors of the polyploid members, homology/diversity of genome(s) within the entire group. We find DNA variation in the hypervariable or multiple copy regions of the genome in Ipomoea species. In addition, we are investigating polymorphism in unique/low copy regions using a battery of DNA sequences from homologous as well as heterologous sources. The success of this study will hopefully shed a new light on the subject of evolutionary biology and may also have potential applications in the sweet potato breeding.

scholarly journals First Report of the Occurrence of Sweet potato leaf curl virus in Tall Morningglory (Ipomoea purpurea) in China

Plant Disease ◽  
2009 ◽  
Vol 93 (7) ◽  
pp. 764-764 ◽  
Author(s):  
C. X. Yang ◽  
Z. J. Wu ◽  
L. H. Xie
Keyword(s):  
Sweet Potato ◽  
Dna Sequences ◽  
Ipomoea Batatas ◽  
Rolling Circle Amplification ◽  
The United States ◽  
Natural Occurrence ◽  
Potato Leaf ◽  
First Report ◽  
Leaf Curl Virus ◽  
Leaf Curl

Natural occurrence of Sweet potato leaf curl virus (SPLCV) has been reported in Ipomoea batatas (sweet potato, Convolvulaceae) or I. indica (Convolvulaceae) in several countries including the United States, Sicily, and China (1–3). In September of 2007, while collecting samples showing begomovirus-like symptoms in the Chinese province of Fujian, we observed tall morningglory (I. purpurea (L.) Roth, also known as Pharbitis purpurea (L.) Voigt), plants with slightly yellow mosaic and crinkled leaves. Total DNA was extracted from leaves of these plants and tested by rolling circle amplification (4). Amplification products were digested by the restriction enzyme BamHI for 30 min. Restriction products (2.8 kb) were then cloned into pMD18T vector (Takara Biotechnology, China) and sequenced. Comparison of complete DNA sequences by Clustal V analysis revealed that these samples were infected by the same virus, and an isolate denoted F-p1 was selected for further sequence analysis. F-p1 was 2,828 nucleotides, with the typical genomic organization of begomoviral DNA-A (GenBank Accession No. FJ515896). F-p1 was compared with the DNA sequences available in the NCBI database using BLAST. The whole DNA sequence showed the highest nucleotide sequence identity (92.1%) with an isolate of SPLCV (GenBank Accession No. FJ176701) from Jiangsu Province of China. The result confirmed that the samples from the symptomatic tall morningglory were infected by SPLCV. To our knowledge, this is the first report of the natural occurrence of SPLCV in I. purpurea, a common weed species in China. References: (1). P. Lotrakul et al. Plant Dis. 82:1253, 1998. (2). R. W. Briddon et al. Plant Pathol. 55:286, 2006. (3) Y. S. Luan et al. Virus Genes 35:379, 2007. (4) D. Haible et al. J. Virol. Methods 135:9, 2006.

scholarly journals Genetic Variability and Host Specialization in the Latin American Clade of Ceratocystis fimbriata

2003 ◽  
Vol 93 (10) ◽  
pp. 1274-1284 ◽  
Author(s):  
Christine J. Baker ◽  
Thomas C. Harrington ◽  
Ulrike Krauss ◽  
Acelino C. Alfenas
Keyword(s):  
Sweet Potato ◽  
Latin American ◽  
Dna Sequences ◽  
Ipomoea Batatas ◽  
Phylogenetic Analyses ◽  
Mangifera Indica ◽  
Its Region ◽  
Host Specialization ◽  
Ceratocystis Fimbriata ◽  
American Clade

The Ceratocystis fimbriata complex includes many undescribed species that cause wilt and canker diseases of many economically important plants. Phylogenetic analyses of DNA sequences have delineated three geographic clades within Ceratocystis fimbriata. This study examined host specialization in the Latin American clade, in which a number of lineages were identified using sequences of the internal transcribed spacer (ITS) region of the rDNA. Three host-associated lineages were identified from cacao (Theobroma cacao), sweet potato (Ipomoea batatas), and sycamore (Platanus spp.), respectively. Isolates from these three lineages showed strong host specialization in reciprocal inoculation experiments on these three hosts. Six cacao isolates from Ecuador, Trinidad, and Columbia differed genetically from other cacao isolates and were not pathogenic to cacao in inoculation tests. Further evidence of host specialization within the Latin American clade of Ceratocystis fimbriata was demonstrated in inoculation experiments in growth chambers using sweet potato, sycamore, Colocasia esculenta, coffee (Coffea arabica), and mango (Mangifera indica) plants; inoculation experiments in Brazil using Brazilian isolates from cacao, Eucalyptus spp., mango, and Gmelina arborea; and inoculation experiments in Costa Rica using Costa Rican isolates from cacao, coffee, and Xantho-soma sp. Hosts native to the Americas appeared to be colonized by only select pathogen genotypes, whereas nonnative hosts were colonized by several genotypes. We hypothesize that local populations of Ceratocystis fimbriata have specialized to different hosts; some of these populations are nascent species, and some host-specialized genotypes have been moved to new areas by humans.

scholarly journals EVALUATION OF ANTIBACTERIAL ACTIVITY OF INDONESIAN VARIETIES SWEET POTATO LEAVES EXTRACT FROM CILEMBU AGAINST Shigella dysenteriae ATCC 13313

2017 ◽  
Vol 10 (2) ◽  
pp. 377 ◽  
Author(s):  
Sri Agung Fitri Kusuma
Keyword(s):  
Antibacterial Activity ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Ethanol Extract ◽  
Biologically Active ◽  
Shigella Dysenteriae ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Dependent Manner ◽  
Sweet Potato Leaves

Objective: The aim of this study was to evaluate the in Vitro antibacterial  activity of sweet potato (Ipomoea batatas (L.) Lam) leaves ethanol extract  against Shigella dysenteriae ATCC 13313.Methods: The study was performed by collecting and determining plant samples, extraction, moisture content assay, phytochemical screening, Thin layer Chromatography, antibacterial activity using agar diffusion method using perforator of 11 mm in diameter and Minimum Inhibition Concentration (MIC) test using macrodilution method against Shigella dysenteriae ATCC 13313.Results: The Phytochemical analysis of the sweet potato leaves extract revealed the presence of  flavonoids, tannin, steroid and polyphenolic compound. The sweet potato leaves ethanol extract showed significant zone of inhibition in a dose dependent manner against Shigella dysenteriae ATCC 13313 with the range of MIC and MBC was 10-20 % b/v.Conclusion : It can be concluded that the sweet potato leaves ethanol extract  shows promise as an antibacterial agent for inhibiting bacillary dysentery due to the presence of biologically active ingredients with antimicrobial activity in the extract.Keywords: sweet potato, Cilembu, Indonesia, antidysentery, Shigella dysenteriae ATCC 13313

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