scholarly journals Detection of Viruses in Sweetpotato from Honduras and Guatemala Augmented by Deep-Sequencing of Small-RNAs

Plant Disease ◽  
2012 ◽  
Vol 96 (10) ◽  
pp. 1430-1437 ◽  
Author(s):  
M. Kashif ◽  
S. Pietilä ◽  
K. Artola ◽  
R. A. C. Jones ◽  
A. K. Tugume ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Virus Strain ◽  
Deep Sequencing ◽  
Ipomoea Batatas ◽  
Plant Viruses ◽  
Virus Infections ◽  
Potato Leaf ◽  
Wide Range ◽  
Virus C ◽  
Small Rna Deep Sequencing

Sweetpotato (Ipomoea batatas) plants become infected with over 30 RNA or DNA viruses in different parts of the world but little is known about viruses infecting sweetpotato crops in Central America, the center of sweetpotato domestication. Small-RNA deep-sequencing (SRDS) analysis was used to detect viruses in sweetpotato in Honduras and Guatemala, which detected Sweet potato feathery mottle virus strain RC and Sweet potato virus C (Potyvirus spp.), Sweet potato chlorotic stunt virus strain WA (SPCSV-WA; Crinivirus sp.), Sweet potato leaf curl Georgia virus (Begomovirus sp.), and Sweet potato pakakuy virus strain B (synonym: Sweet potato badnavirus B). Results were confirmed by polymerase chain reaction and sequencing of the amplicons. Four viruses were detected in a sweetpotato sample from the Galapagos Islands. Serological assays available to two of the five viruses gave results consistent with those obtained by SRDS, and were negative for six additional sweetpotato viruses tested. Plants coinfected with SPCSV-WA and one to two other viruses displayed severe foliar symptoms of epinasty and leaf malformation, purpling, vein banding, or chlorosis. The results suggest that SRDS is suitable for use as a universal, robust, and reliable method for detection of plant viruses, and especially useful for determining virus infections in crops infected with a wide range of unrelated viruses.

scholarly journals Effects of Process Parameters on the Color Quality of Anthocyanin-Based Colorants from Conventional and Microwave-Assisted Aqueous Extraction of Sweet Potato (Ipomoea batatas L.) Leaf Varieties

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 103
Author(s):  
Jin Mark D. G. Pagulayan ◽  
Aprille Suzette V. Mendoza ◽  
Fredelyn S. Gascon ◽  
Jan Carlo C. Aningat ◽  
Abigail S. Rustia ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Process Parameters ◽  
Ipomoea Batatas ◽  
Extraction Methods ◽  
Aqueous Extraction ◽  
Potato Leaf ◽  
Microwave Assisted ◽  
Color Quality ◽  
The Impact

The study aimed to evaluate the effects of process parameters (time and raw material weight (RMW)) of conventional (boiling for 10–45 min) and microwave-assisted (2–8 min) aqueous extraction on the color quality (i.e., lightness (L*), chroma (C*), and hue (H°) of anthocyanin –based colorants of red and Inubi sweet potato (Ipomoea batatas L.) leaves. Using response surface methodology, it was found that RMW and boiling time (BT) and microwave time (MT) generally had a significant (p < 0.05) effect on the color quality of the extract from both extraction methods. The effects were found to vary depending on the extraction method and variety of the leaves used. Both extraction methods produced a brown to brick-red extract from the Inubi variety that turned red-violet to pink when acidified. The red sweet potato leaves produced a deep violet colored extract that also turned red-violet when acidified. It is recommended that the anthocyanin content of the extracts be measured to validate the impact of the methods on the active agent. Nevertheless, the outcomes in this study may serve as baseline data for further studies on the potential of sweet potato leaf colorants (SPLC) as a colorant with functional properties.

Identification of viruses infecting sweet potato in southern China by small RNA deep sequencing and PCR detection

2018 ◽  
Vol 85 (2) ◽  
pp. 122-127 ◽  
Author(s):  
Siqi Ma ◽  
Qiufeng Zheng ◽  
Jiajie Ye ◽  
Wendi Feng ◽  
Guohui Zhou ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Deep Sequencing ◽  
Small Rna ◽  
Southern China ◽  
Pcr Detection ◽  
Rna Deep Sequencing ◽  
Small Rna Deep Sequencing

scholarly journals The antihyperlipidaemic and hepatoprotective effect of Ipomoea batatas L. leaves extract in high-fat diet rats

2021 ◽  
Vol 10 (3) ◽  
pp. 558
Author(s):  
Nurkhasanah Mahfudh ◽  
Nanik Sulistyani ◽  
Muhammad Syakbani ◽  
Athifah Candra Dewi
Keyword(s):  
Sweet Potato ◽  
High Fat Diet ◽  
Ipomoea Batatas ◽  
Lipid Level ◽  
Hepatoprotective Effect ◽  
High Fat ◽  
Potato Leaf ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Liver Histopathology ◽  
Purple Sweet Potato

The administration of high-fat diets can increase the body's lipid level and damage the organs. Purple sweet potato leaf (Ipomoea batatas L.) was reported as an antioxidant against free radicals. This study aimed to observe the sweet potato leaf extract's activity on decreasing lipid profile and hepatoprotective effect in high-fat diet fed rats. The treatment animals were divided into five groups, namely normal control, high-fat diet (HFD) control, the treatment group of purple sweet potato leaf extract (SPLE) doses 100 mg/kg BW, 200 mg/kg BW and 400 mg/kg BW which fed with high-fat diet for 14 days and SPLE for 28 days. After treatment was completed, the blood was collected for the detection of cholesterol, triglyceride, serum glutamic oxaloacetic transaminase (SGOT), and serum glutamic pyruvate transaminase (SGPT). After that, the animals were sacrificed, and a liver histopathology observation was conducted using Haematoxylien and Eosin staining. The result showed a significant decrease in cholesterol and triglyceride levels (p≤0.05) compared to the negative group in all treated groups. The SGOT and SGPT enzymes in all of treatment groups were also found to decrease compared with HFD control. The result was confirmed by the histopathological observations. The finding suggested the potency of SPLE for antihyperlipidaemic and hepatoprotective agent.

scholarly journals High-throughput deep sequencing reveals the important role that microRNAs play in the salt response in sweet potato (Ipomoea batatas L.)

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhengmei Yang ◽  
Panpan Zhu ◽  
Hunseung Kang ◽  
Lin Liu ◽  
Qinghe Cao ◽  
...  
Keyword(s):  
Sweet Potato ◽  
High Throughput ◽  
Deep Sequencing ◽  
Ipomoea Batatas ◽  
Salt Response

scholarly journals Plant Virus Metagenomics: Advances in Virus Discovery

2015 ◽  
Vol 105 (6) ◽  
pp. 716-727 ◽  
Author(s):  
Marilyn J. Roossinck ◽  
Darren P. Martin ◽  
Philippe Roumagnac
Keyword(s):  
Nucleic Acids ◽  
Deep Sequencing ◽  
Plant Virus ◽  
Virus Disease ◽  
Plant Viruses ◽  
Wild Plants ◽  
Cultivated Plants ◽  
Virus Discovery ◽  
Virus Infections ◽  
Virus Like Particles

In recent years plant viruses have been detected from many environments, including domestic and wild plants and interfaces between these systems—aquatic sources, feces of various animals, and insects. A variety of methods have been employed to study plant virus biodiversity, including enrichment for virus-like particles or virus-specific RNA or DNA, or the extraction of total nucleic acids, followed by next-generation deep sequencing and bioinformatic analyses. All of the methods have some shortcomings, but taken together these studies reveal our surprising lack of knowledge about plant viruses and point to the need for more comprehensive studies. In addition, many new viruses have been discovered, with most virus infections in wild plants appearing asymptomatic, suggesting that virus disease may be a byproduct of domestication. For plant pathologists these studies are providing useful tools to detect viruses, and perhaps to predict future problems that could threaten cultivated plants.

scholarly journals PENGARUH JENIS PELARUT TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN UBI JALAR PUTIH (Ipomoea batatas L) MENGGUNAKAN METODE MASERASI

2020 ◽  
Vol 9 (4) ◽  
pp. 438
Author(s):  
Ade Maria Kristin Gultom ◽  
Ni Made Yusa ◽  
Anak Agung Istri Sri Wiadnyani
Keyword(s):  
Antioxidant Activity ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Leaf Extract ◽  
Potato Leaf ◽  
Measured Variable ◽  
Obvious Effect ◽  
Randomized Design ◽  
High Antioxidant Activity ◽  
Sweet Potato Leaves

This study aims to determine the effect of solvent types on antioxidant activity of white sweet potato leaf extract (Ipomoea batatas L) and to obtain the most appropriate type of solvent to produce white sweet potato leaf extract with high antioxidant activity. The experimental design uses in this research was Completely Randomized Design (CRD) with the treatment of solvent types methanol 80%, acetone 80%, ethanol 80% and aquades. The treatment was repeated four times so obtain 16 units of the experimental. Data were analysis of variance and followed by Duncan test if the treatment has a significant effect on measured variable. The results showed that, type of solvent had an obvious effect on antioxidant activity of white sweet potato leaves. Methanol was the best solvent in extract white sweet potato leaves with followed by yield was 32.11%, total flavonoids was 226.45 mg QE / g, total tannin was 16.58 mg TAE / g, vitamin C was 119.42 mg AAE / g and antioxidant activity was 82.42%.

scholarly journals Caracterización de la raza Sweet potato feathery mottle virus que afecta a Ipomoea batatas (L.) Lam. en Argentina y establecimiento de un método rápido de diagnóstico en plantas in vitro

2017 ◽  
Author(s):  
◽  
Paola Analía Faroni
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Mottle Virus ◽  
Ipomoea Nil ◽  
Virus C ◽  
Das Elisa

El cultivo de batata en Argentina ha experimentado una disminución en la superficie plantada y, por ende, en su producción. Actualmente, todas las regiones productoras de batata se encuentran afectadas por una la patología viral denominada “encrespamiento amarillo” (EA), la más grave que se haya presentado hasta la actualidad en el país. El EA es causado por cinco virus, dentro de los cuales se encuentran los potyvirus, Virus C de la batata (SPVC) y Virus del moteado plumoso de la batata (SPFMV) razas RC y O. Estos en infecciones simples no ocasionan problemas en el cultivo, pero en las mixtas llegan a causar mermas en rendimientos superiores al 80%.En el presente trabajo se caracterizó biológica, serológicamente y molecularmente a SPVC que pudo ser aislado de plantas infectadas con SPFMV, otro potyvirus estrechamente relacionado con él. SPVC fue transmitido mediante injerto y mediante inoculación mecánica, en bajo porcentaje en Ipomoea nil e I. setosa. En infecciones simples, se produjo síntomas típicos de infección con SPFMV, en hojas de I. setosa (hospedante alternativo). Complementariamente, se logró purificar el virus y posteriormente se obtuvo el antisuero para el diagnóstico de SPVC, mediante DAS-ELISA, NCM-ELISA e ISEM +D. Los ensayos biológicos y/o serológicos no logran diferenciar SPFMV y SPVC cuando las plantas están infectadas, no resultando fiables para su diagnóstico. Ensayos basados en ácidos nucleícos proporcionan la ventaja de una detección confiable de virus, siendo la sonda de hibridación un instrumento específico para diagnóstico. El presente estudio permitió obtener una sonda de hibridación para detectar específicamente al Sweetpotato virus C, aunque son necesarias pruebas ulteriores que permitan concluir al respecto.

scholarly journals Effect of Sweet Potato Leaf Supplementation on Growth and Nutrient Digestibility in Sheep

2020 ◽  
Vol 51 (2) ◽  
pp. 51-57
Author(s):  
A. Melesse ◽  
N. Chalew ◽  
A. Nurfeta
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Nutrient Digestibility ◽  
Potato Leaf ◽  
N Retention ◽  
Natural Grass ◽  
N Utilization ◽  
Grass Hay

AbstractThe effects of feeding dried sweet potato (Ipomoea batatas) leaf (SPL) on growth performance, digestibility, and nitrogen (N) utilization were studied in sheep. Twenty-four rams with initial body weight of 18.5 ± 1.49 kg were randomly allocated to four treatments containing ad libitum natural grass hay alone (T1), hay + 150 g SPL (T2), hay + 300 g SPL (T3) and hay + 450 g SPL (T4) on as fed basis. Results indicated that dry matter (DM), organic matter (OM) and crude protein (CP) in-takes increased (P < 0.001) with increasing levels of SPL. Sheep fed with T2, T3 and T4 diets gained 55.1, 52.6 and 66.5 g per head per day, respectively, while the gain of non-supplemented sheep (9.19 g per head per day) differed (P < 0.05). Digestibility of DM, OM, and CP was higher (P < 0.01) for all supplemented sheep compared with the control. The CP digestibility and N retention were negative in non-supplemented sheep. Urinary N excretion decreased (P < 0.001) with the increasing levels of SPL supplementation. The N retention improved with the increasing levels of SPL and was the highest (P < 0.001) in sheep fed with T4 diet and the lowest in non-supplemented sheep. In conclusion, supplementing a basal diet of natural grass hay with SPL considerably improved the average daily gain, OM and CP digestibility and N retention.

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