scholarly journals Draft Genome Resource for the Ex-types of Phytophthora ramorum, P. kernoviae, and P. melonis, Species of Regulatory Concern, Using Ultra–Long Read MinION Nanopore Sequencing

2020 ◽  
Vol 33 (6) ◽  
pp. 794-797
Author(s):  
Subodh K. Srivastava ◽  
Z. Gloria Abad ◽  
Leandra M. Knight ◽  
Kurt Zeller ◽  
Vessela Mavrodieva ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Draft Genome ◽  
The United States ◽  
Phytophthora Ramorum ◽  
Type Material ◽  
Whole Genome Sequence ◽  
Diagnostic Systems ◽  
Type Specimens ◽  
Genome Wide ◽  
The World

Phytophthora ramorum, P. kernoviae, and P. melonis are each species of current regulatory concern in the United States, the United Kingdom, and other areas of the world. Ex-type material are cultures and duplicates of the type that was used to describe each species and that are deposited in additional culture collections. Using these type specimens as references is essential to designing correct molecular identification and diagnostic systems. Here, we report a whole genome sequence for the Ex-type material of P. ramorum, P. kernoviae, and P. melonis generated using high-throughput sequencing via the MinION third generation platform from Oxford Nanopore Technology. We assembled the quality filtered reads into contigs for each species. We assembled the continuous contigs of P. ramorum, P. kernoviae, and P. melonis (1,322, 545, and 2,091 contigs, respectively). The ab initio prediction of genes from these species reveals that there are 16,838, 12,793, and 34,580 genes in P. ramorum, P. kernoviae, and P. melonis, respectively. Of the 34,580 P. melonis genes, 10,164 genes were conserved among all three of these Phytophthora species which may include pathogenicity genes. We compared the ex-type of P. ramorum EU1 lineage assembly with another selected isolate of EU1 available at the National Center for Biotechnology Information and found 251,859 single nucleotide polymorphisms (SNPs) genome-wide; the comparison with the EU2 lineage genome isolate revealed 441,859 SNPs genome-wide. This genome resource of the ex-types of P. ramorum, and P. kernoviae is a significant contribution as these species are among the most important pathogens of regulatory concern in different regions of the world.

scholarly journals Phytophthora Genomics: The Plant Destroyers' Genome Decoded

2006 ◽  
Vol 19 (12) ◽  
pp. 1295-1301 ◽  
Author(s):  
Francine Govers ◽  
Mark Gijzen
Keyword(s):  
Gene Annotation ◽  
Draft Genome ◽  
The United States ◽  
Phytophthora Species ◽  
Phytophthora Sojae ◽  
Phytophthora Ramorum ◽  
Department Of Energy ◽  
United States Department ◽  
Sequencing Project ◽  
Energy Joint Genome Institute

The year 2004 was an exciting one for the Phytophthora research community. The United States Department of Energy Joint Genome Institute (JGI) completed the draft genome sequence of two Phytophthora species, Phytophthora sojae and Phytophthora ramorum. In August of that year over 50 people gathered at JGI in Walnut Creek, California, for an annotation jamboree and searched for the secrets and surprises that the two genomes have in petto. This culminated in a paper in Science in September of this year describing the highlights of the sequencing project and emphasizing the power of having the genome sequences of two closely related organisms. This MPMI Focus issue on Phytophthora genomics contains a number of more specialized manuscripts centered on gene annotation and genome organization, and complemented with manuscripts that rely on genomics resources.

scholarly journals Screening of grapevine red blotch virus in two European ampelographic collections

2021 ◽  
Author(s):  
Jean-Sébastien Reynard ◽  
Justine Brodard ◽  
Nathalie Dubuis ◽  
Isabelle Kellenberger ◽  
Anne-Sophie Spilmont ◽  
...  
Keyword(s):  
North America ◽  
Vitis Vinifera ◽  
Large Scale ◽  
High Throughput Sequencing ◽  
The United States ◽  
Dna Viruses ◽  
Unintentional Movement ◽  
Host Jump ◽  
The World ◽  
Different Origins

AbstractGrapevine red blotch virus (GRBV) is a recently identified virus that infects grapevine and has a severe impact on the grape industry in North America. Since the first description of the virus 8 years ago, clear progress has been made regarding our understanding of the GRBV pathosystem. However, questions remain regarding the origin of this pathogen and its spread outside North America, especially in Europe. In this study, we present the results of a large-scale GRBV survey in two European repositories; we targeted Vitis spp. accessions with diverse geographical origins. Of 816 accessions from different origins (50 different countries around the world), six accessions were infected by GRBV, all of which originated from the United States. We investigated the DNA virome of 155 grapevine accessions from the Swiss grapevine collection using high-throughput sequencing. We observed that virome of the Swiss grapevine collection was composed of several RNA viruses. In contrast, we did not detect any DNA viruses in the 155 Swiss grapevine accessions. This finding suggests that the abundance of DNA viruses infecting grapevines in Switzerland is either very low or non-existent. Our results and the findings of studies published since 2008 show that GRBV most likely originated in North America and subsequently spread to other viticultural areas in the world via unintentional movement of infected cuttings. According to our data, the most plausible scenario for the origin of GRBV is that the virus evolved from non-Vitis vinifera hosts and underwent a host jump to Vitis vinifera after its introduction to North America in the 1600s.

scholarly journals Human Infections Caused by Clonally Related African Clade (Clade III) Strains of Candida auris in the Greater Houston Region

2020 ◽  
Vol 58 (7) ◽  
Author(s):  
S. Wesley Long ◽  
Matthew Ojeda Saavedra ◽  
Paul A. Christensen ◽  
James M. Musser ◽  
Randall J. Olsen
Keyword(s):  
United States ◽  
Genome Sequence ◽  
Clinical Laboratory ◽  
Draft Genome ◽  
The United States ◽  
Metropolitan Region ◽  
Whole Genome Sequence ◽  
Candida Auris ◽  
Content Type ◽  
African Clade

ABSTRACT Candida auris is a pathogen of considerable public health importance. It was first reported in 2009. Five clades, determined by genomic analysis and named by the distinct regions where they were initially identified, have been defined. We previously completed a draft genome sequence of an African clade (clade III) strain cultured from the urine of a patient hospitalized in the greater Houston metropolitan region (strain LOM). Although initially uncommon, reports of the African clade in the United States have grown to include a recent cluster in California. Here, we describe a second human C. auris infection in the Houston area. Whole-genome sequence analysis demonstrated the Houston patient isolates to be clonally related to one another but distantly related to other African clade organisms recovered in the United States or elsewhere. Infections in these patients were present on admission to the hospital and occurred several months apart. Taken together, the data demonstrate the emergence and persistence of a clonal C. auris population and highlights the importance of routine high-resolution genomic surveillance of emerging human pathogens in the clinical laboratory.

International Telesupervision: The Clinical Fellowship Experience

2017 ◽  
Vol 2 (11) ◽  
pp. 73-78
Author(s):  
David W. Rule ◽  
Lisa N. Kelchner
Keyword(s):  
Graduate Students ◽  
Clinical Supervision ◽  
Rural Areas ◽  
The United States ◽  
Speech Language Pathology ◽  
Access To Services ◽  
East African ◽  
The World ◽  
Language Pathology ◽  
National Certification

Telepractice technology allows greater access to speech-language pathology services around the world. These technologies extend beyond evaluation and treatment and are shown to be used effectively in clinical supervision including graduate students and clinical fellows. In fact, a clinical fellow from the United States completed the entire supervised clinical fellowship (CF) year internationally at a rural East African hospital, meeting all requirements for state and national certification by employing telesupervision technology. Thus, telesupervision has the potential to be successfully implemented to address a range of needs including supervisory shortages, health disparities worldwide, and access to services in rural areas where speech-language pathology services are not readily available. The telesupervision experience, potential advantages, implications, and possible limitations are discussed. A brief guide for clinical fellows pursuing telesupervision is also provided.

Sex Differences in Mass Media Preferences Across Four Asian Countries

2011 ◽  
Vol 23 (4) ◽  
pp. 186-191 ◽  
Author(s):  
Malini Ratnasingam ◽  
Lee Ellis
Keyword(s):  
United States ◽  
Sex Differences ◽  
Mass Media ◽  
The United States ◽  
Female Students ◽  
The Internet ◽  
Evolutionary Explanation ◽  
Asian Countries ◽  
The World ◽  
Media Preferences

Background. Nearly all of the research on sex differences in mass media utilization has been based on samples from the United States and a few other Western countries. Aim. The present study examines sex differences in mass media utilization in four Asian countries (Japan, Malaysia, South Korea, and Singapore). Methods. College students self-reported the frequency with which they accessed the following five mass media outlets: television dramas, televised news and documentaries, music, newspapers and magazines, and the Internet. Results. Two significant sex differences were found when participants from the four countries were considered as a whole: Women watched television dramas more than did men; and in Japan, female students listened to music more than did their male counterparts. Limitations. A wider array of mass media outlets could have been explored. Conclusions. Findings were largely consistent with results from studies conducted elsewhere in the world, particularly regarding sex differences in television drama viewing. A neurohormonal evolutionary explanation is offered for the basic findings.

Sandra Ramos Revisited

2020 ◽  
Vol 2 (4) ◽  
pp. 32-54
Author(s):  
Silvia Spitta
Keyword(s):  
United States ◽  
The United States ◽  
Ancient Greek ◽  
Graphic Arts ◽  
Left Behind ◽  
The World ◽  
The Usa ◽  
Political Choices ◽  
The Rich

Sandra Ramos (b. 1969) is one of the few artists to reflect critically on both sides of the Cuban di-lemma, fully embodying the etymological origins of the word in ancient Greek: di-, meaning twice, and lemma, denoting a form of argument involving a choice between equally unfavorable alternatives. Throughout her works she shines a light on the dilemmas faced by Cubans whether in Cuba or the United States, underlining the bad personal and political choices people face in both countries. During the hard 1990s, while still in Havana, the artist focused on the traumatic one-way journey into exile by thousands, as well as the experience of profound abandonment experienced by those who were left behind on the island. Today she lives in Miami and operates a studio there as well as one in Havana. Her initial disorientation in the USA has morphed into an acerbic representation and critique of the current administration and a deep concern with the environmental collapse we face. A buffoonlike Trumpito has joined el Bobo de Abela and Liborio in her gallery of comic characters derived from the rich Cuban graphic arts tradition where she was formed. While Cuba is now represented as a rotten cake with menacing flies hovering over it ready to pounce, a bombastic Trumpito marches across the world stage, trampling everything underfoot, a dollar sign for a face.

The 1970s

2011 ◽  
Author(s):  
Thomas Borstelmann
Keyword(s):  
Civil Rights ◽  
Free Market ◽  
The United States ◽  
Global Perspective ◽  
Retirement Plans ◽  
Free Agency ◽  
The World ◽  
The Disabled ◽  
Oil Crisis ◽  
American Society

This book looks at an iconic decade when the cultural left and economic right came to the fore in American society and the world at large. While many have seen the 1970s as simply a period of failures epitomized by Watergate, inflation, the oil crisis, global unrest, and disillusionment with military efforts in Vietnam, this book creates a new framework for understanding the period and its legacy. It demonstrates how the 1970s increased social inclusiveness and, at the same time, encouraged commitments to the free market and wariness of government. As a result, American culture and much of the rest of the world became more—and less—equal. This book explores how the 1970s forged the contours of contemporary America. Military, political, and economic crises undercut citizens' confidence in government. Free market enthusiasm led to lower taxes, a volunteer army, individual 401(k) retirement plans, free agency in sports, deregulated airlines, and expansions in gambling and pornography. At the same time, the movement for civil rights grew, promoting changes for women, gays, immigrants, and the disabled. And developments were not limited to the United States. Many countries gave up colonial and racial hierarchies to develop a new formal commitment to human rights, while economic deregulation spread to other parts of the world, from Chile and the United Kingdom to China. Placing a tempestuous political culture within a global perspective, this book shows that the decade wrought irrevocable transformations upon American society and the broader world that continue to resonate today.

The Unquiet Frontier

2017 ◽  
Author(s):  
Jakub J. Grygiel ◽  
A. Wess Mitchell ◽  
Jakub J. Grygiel ◽  
A. Wess Mitchell
Keyword(s):  
United States ◽  
The United States ◽  
Great Powers ◽  
Global Order ◽  
American Power ◽  
Alliance Networks ◽  
Effective Strategies ◽  
The World ◽  
The Baltic ◽  
Critical Regions

From the Baltic to the South China Sea, newly assertive authoritarian states sense an opportunity to resurrect old empires or build new ones at America's expense. Hoping that U.S. decline is real, nations such as Russia, Iran, and China are testing Washington's resolve by targeting vulnerable allies at the frontiers of American power. This book explains why the United States needs a new grand strategy that uses strong frontier alliance networks to raise the costs of military aggression in the new century. The book describes the aggressive methods which rival nations are using to test American power in strategically critical regions throughout the world. It shows how rising and revisionist powers are putting pressure on our frontier allies—countries like Poland, Israel, and Taiwan—to gauge our leaders' commitment to upholding the American-led global order. To cope with these dangerous dynamics, nervous U.S. allies are diversifying their national-security “menu cards” by beefing up their militaries or even aligning with their aggressors. The book reveals how numerous would-be great powers use an arsenal of asymmetric techniques to probe and sift American strength across several regions simultaneously, and how rivals and allies alike are learning from America's management of increasingly interlinked global crises to hone effective strategies of their own. The book demonstrates why the United States must strengthen the international order that has provided greater benefits to the world than any in history.

Bringing the Real World of Science to Children: A Partnership of the American Museum of Natural History and the City University of New York

2008 ◽  
Vol 12 (1) ◽  
Author(s):  
Anthony G Picciano ◽  
Robert V. Steiner
Keyword(s):  
United States ◽  
New York ◽  
Natural History ◽  
Local Governments ◽  
Compulsory Education ◽  
Development Program ◽  
The United States ◽  
American Museum ◽  
The World ◽  
The City

Every child has a right to an education. In the United States, the issue is not necessarily about access to a school but access to a quality education. With strict compulsory education laws, more than 50 million students enrolled in primary and secondary schools, and billions of dollars spent annually on public and private education, American children surely have access to buildings and classrooms. However, because of a complex and competitive system of shared policymaking among national, state, and local governments, not all schools are created equal nor are equal education opportunities available for the poor, minorities, and underprivileged. One manifestation of this inequity is the lack of qualified teachers in many urban and rural schools to teach certain subjects such as science, mathematics, and technology. The purpose of this article is to describe a partnership model between two major institutions (The American Museum of Natural History and The City University of New York) and the program designed to improve the way teachers are trained and children are taught and introduced to the world of science. These two institutions have partnered on various projects over the years to expand educational opportunity especially in the teaching of science. One of the more successful projects is Seminars on Science (SoS), an online teacher education and professional development program, that connects teachers across the United States and around the world to cutting-edge research and provides them with powerful classroom resources. This article provides the institutional perspectives, the challenges and the strategies that fostered this partnership.

Sign in / Sign up

Export Citation Format

Share Document