scholarly journals The Occurrence of PVYO, PVYN, and PVYN:O Strains of Potato virus Y in Certified Potato Seed Lot Trials in Washington and Oregon

Plant Disease ◽  
2006 ◽  
Vol 90 (8) ◽  
pp. 1102-1105 ◽  
Author(s):  
J. M. Crosslin ◽  
P. B. Hamm ◽  
D. C. Hane ◽  
J. Jaeger ◽  
C. R. Brown ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
General Trend ◽  
Potato Production ◽  
The United States ◽  
Yield Reduction ◽  
Potato Seed ◽  
Seed Origin ◽  
Canadian Provinces ◽  
Certified Potato Seed

Totals of 960 and 286 certified potato seed lots from locations across North America were planted in trials in Washington and Oregon, respectively, in 2001 to 2003 and tested for strains of Potato virus Y (PVY). The incidence of PVYO-infected lots averaged 16.4 and 25.9% in the Washington and Oregon trials, respectively. There was a general trend of increasing incidence of the PVYO, PVYN:O, and PVYN strains during this period, as evidenced by more infected cultivars, sites of seed origin, and number of seed growers providing infected seed lots. In particular, there was a dramatic increase in seed lots with the PVYN:O strain from 2002 to 2003. PVYN:O, in contrast to PVYO, which only causes yield reduction, also causes internal and external damage to tubers, making them unmarketable. In 2003, PVYN:O occurred in seed lots originating in eight states and three Canadian provinces. The increased incidence of PVYN:O was likely due to the difficulty in differentiating this strain from PVYO. The prevalence of PVY in potato seed lots documented herein poses a threat to potato production in the United States and suggests that current measures to reduce the incidence of this virus are inadequate.

scholarly journals Transmission Efficiency of Potato virus Y strains PVYO and PVYN-Wi by Five Aphid Species

Plant Disease ◽  
2011 ◽  
Vol 95 (10) ◽  
pp. 1279-1283 ◽  
Author(s):  
A. F. S. Mello ◽  
R. A. Olarte ◽  
S. M. Gray ◽  
K. L. Perry
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Rhopalosiphum Padi ◽  
Potato Production ◽  
Aphid Transmission ◽  
Transmission Efficiency ◽  
Aphid Species ◽  
Age And Growth ◽  
Potato Seed ◽  
True Potato Seed

Potato virus Y (PVY) is a reemerging problem in potato production in North America. Although the “ordinary” strain, PVYO, is still the dominant isolate in U.S. seed potatoes, the recombinant strain of the virus PVYN-Wi (= PVYN:O) has become widespread. An increase in the prevalence of a PVY strain could be due to differences in the efficiency of transmission by aphid vectors. The transmission efficiency by a clone of Myzus persicae was determined for five isolates each of PVYO and PVYN-Wi. An aphid transmission assay was developed based on the use of potato seedlings from true potato seed, allowing for greater control of plant age and growth stage. No apparent differences in transmission by M. persicae were observed. Single isolates of PVYO and PVYN-Wi were tested for their ability to be transmitted from potato to potato by five aphid species: Aphis glycines, A. gossypii, A. nasturtii, M. persicae, and Rhopalosiphum padi. Both PVY isolates showed a similar transmission phenotype in being transmitted efficiently by M. persicae but very poorly or not at all by A. glycines, A. gossypii, and R. padi. The aphid A. nasturtii transmitted both isolates with an intermediate level of efficiency. The data do not support a model for a differential aphid transmissibility being responsible for the increase in the prevalence of PVYN-Wi.

scholarly journals Strain-Specific Resistance to Potato virus Y (PVY) in Potato and Its Effect on the Relative Abundance of PVY Strains in Commercial Potato Fields

Plant Disease ◽  
2017 ◽  
Vol 101 (1) ◽  
pp. 20-28 ◽  
Author(s):  
Cassandra N. Funke ◽  
Olga V. Nikolaeva ◽  
Kelsie J. Green ◽  
Lisa T. Tran ◽  
Mohamad Chikh-Ali ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Specific Resistance ◽  
Potato Production ◽  
The United States ◽  
Potato Cultivars ◽  
Columbia Basin ◽  
Recombinant Strains ◽  
Systemic Movement ◽  
Production Area

Potato virus Y (PVY) is a serious threat to potato production due to effects on tuber yield and quality, in particular, due to induction of potato tuber necrotic ringspot disease (PTNRD), typically associated with recombinant strains of PVY. These recombinant strains have been spreading in the United States for the past several years, although the reasons for this continuing spread remained unclear. To document and assess this spread between 2011 and 2015, strain composition of PVY isolates circulating in the Columbia Basin potato production area was determined from hundreds of seed lots of various cultivars. The proportion of nonrecombinant PVYO isolates circulating in Columbia Basin potato dropped ninefold during this period, from 63% of all PVY-positive plants in 2011 to less than 7% in 2015. This drop in PVYO was concomitant with the rise of the recombinant PVYN-Wi strain incidence, from less than 27% of all PVY-positive plants in 2011 to 53% in 2015. The proportion of the PVYNTN recombinant strain, associated with PTNRD symptoms in susceptible cultivars, increased from 7% in 2011 to approximately 24% in 2015. To further address the shift in strain abundance, screenhouse experiments were conducted and revealed that three of the four most popular potato cultivars grown in the Columbia Basin exhibited strain-specific resistance against PVYO. Reduced levels of systemic movement of PVYO in such cultivars would favor spread of recombinant strains in the field. The negative selection against the nonrecombinant PVYO strain is likely caused by the presence of the Nytbr gene identified in potato cultivars in laboratory experiments. Presence of strain-specific resistance genes in potato cultivars may represent the driving force changing PVY strain composition to predominantly recombinant strains in potato production areas.

scholarly journals Distribution and incidence of viruses in Irish seed potato crops

2015 ◽  
Vol 54 (2) ◽  
pp. 98-106 ◽  
Author(s):  
F. Hutton ◽  
J.H. Spink ◽  
D. Griffin ◽  
S. Kildea ◽  
D. Bonner ◽  
...  
Keyword(s):  
Potato Virus ◽  
Significant Proportion ◽  
Potato Production ◽  
Weather Data ◽  
Management Approach ◽  
Potato Seed ◽  
Virus Diseases ◽  
Aphid Control ◽  
Daily Weather Data ◽  
Potato Crops

Abstract Virus diseases are of key importance in potato production and in particular for the production of disease-free potato seed. However, there is little known about the frequency and distribution of potato virus diseases in Ireland. Despite a large number of samples being tested each year, the data has never been collated either within or across years. Information from all known potato virus testing carried out in the years 2006–2012 by the Department of Agriculture Food and Marine was collated to give an indication of the distribution and incidence of potato virus in Ireland. It was found that there was significant variation between regions, varieties, years and seed classes. A definition of daily weather data suitable for aphid flight was developed, which accounted for a significant proportion of the variation in virus incidence between years. This use of weather data to predict virus risk could be developed to form the basis of an integrated pest management approach for aphid control in Irish potato crops.

Effect of Potato virus Y on Yield of Three Potato Cultivars Grown Under Different Nitrogen Levels

Plant Disease ◽  
2006 ◽  
Vol 90 (1) ◽  
pp. 73-76 ◽  
Author(s):  
Jonathan L. Whitworth ◽  
Phil Nolte ◽  
Chris McIntosh ◽  
Robert Davidson
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Total Yield ◽  
Russet Burbank ◽  
Yield Reduction ◽  
Symptom Expression ◽  
Russet Norkotah ◽  
Nitrogen Levels ◽  
Foliar Symptoms ◽  
Significant Yield

Previous studies have shown that Potato virus Y (PVY) reduces yield in many cultivars. Typical foliar symptoms can include veinal necrosis, leaf drop, and a mosaic pattern sometimes accompanied by leaf roughness. Infection by PVY in Russet Burbank produces identifiable PVY symptoms, whereas cv. Russet Norkotah expresses mild, almost latent symptoms. Yield also is influenced by nitrogen fertilizer levels. This research was conducted to determine whether increased nitrogen mitigates yield reduction caused by PVY. Russet Norkotah, CO80011-5, and Russet Burbank were used in replicated plots of non-PVY-infected and PVY-infected plants at three nitrogen levels in 1995 and 1996. There was a significant yield reduction between PVY-negative and PVY-positive plots in all cultivars, at most nitrogen levels. PVY yield reduction was similar (approximately 38%) in the mild symptom expression clones of Russet Norkotah and CO80011-5, whereas the yield reduction in Russet Burbank, which exhibits typical symptom expression, was 63.5%. We conclude that increased nitrogen can influence total yield, but does not significantly mitigate the yield reduction due to PVY infection.

Prevalence and strain composition of potato virus Y circulating in potato fields in China’s north-central province Shanxi

Plant Disease ◽  
2021 ◽  
Author(s):  
Pengcheng Ding ◽  
Dexin Chen ◽  
Haixu Feng ◽  
Jiao Li ◽  
Hui Cao ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Production ◽  
Central China ◽  
Shanxi Province ◽  
Rt Pcr ◽  
Potato Leaf ◽  
Single Strain ◽  
North Central ◽  
Production Areas

Potato is an important crop in Shanxi province located in north-central China. During 2019-2020, 319 potato leaf samples were collected from eight locations distributed in three major potato production areas in Shanxi. Bio-chip detection kit revealed the presence of several potato viruses, and among them potato virus Y (PVY) was the most common one, reaching the incidence of 87.8% of all symptomatic samples. The immuno-captured multiplex reverse transcription (RT)-PCR was used to identify strains for all 280 PVY-positive samples, unveiling 242 samples infected with a single strain of PVY (86.4%) and 38 (13.6%) with a mixed infection. Of samples with a single-strain infection, PVY -SYR-II accounted for 102 (42.1%), followed by PVYN-Wi (33, 13.6%) , PVY -SYR-I (28, 11.6%), 261-4 (22, 9.1%), PVYNTNa (20, 8.3%), PVYNTNb (19, 7.9%), and PVY -SYR-III (18, 7.4%). Seven isolates representing different recombinants were selected for whole genome sequencing. Phylogenetic and recombination analyses confirmed the RT-PCR based strain typing for all seven strains of PVY found in Shanxi. SXKL-12 is the first SYR-III strain from potato reported from China. However, unlike that in other known SYR-III isolates, the region positioned from 1,764 to1,902 nt in SXKL-12 shared the highest sequence identity of 82.2% with an uncharacterized PVY isolate, JL-23, from China. Interestingly, the PVYN-Wi isolate SXZY-40 also possessed a more divergent sequence for the region positioned from 6,156 to 6,276 nt than other N-Wi isolates known to date, sharing the highest identity of 86.6% with an uncharacterized Chinese PVY isolate, JL-11. Pathogenicity analysis of dominant strains PVY -SYR-II and PVYN-Wi in six local popular potato cultivars revealed that Kexin 13, Helan 15 and Jizhangshu 12 were susceptible to these two strains with mild mottling or mosaic symptoms expression, while three cultivars, Jinshu 16, Qingshu 9, Xisen 6 were found fully resistant.

scholarly journals Multiple DNA markers for evaluation of resistance against Potato virus Y, Potato virus S and Potato leafroll virus

2018 ◽  
Vol 54 (No. 1) ◽  
pp. 30-33 ◽  
Author(s):  
M. Naderpour ◽  
L. Sadeghi
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Simultaneous Detection ◽  
Potato Leafroll Virus ◽  
Potato Seed ◽  
Breeding Programs ◽  
Potato Virus S ◽  
Sequence Characterized Amplified Region ◽  
Leafroll Virus ◽  
Resistance To Viruses

Molecular markers within or close to genes of interest play essential roles in marker-assisted selection. PCR-based markers have been developed for numerous traits in different plant species including several genes conferring resistance to viruses in potato. In the present work, rapid and reliable approaches were developed for the simultaneous detection of Ryadg and Ry-fsto, Ns, and PLRV.1 genes conferring resistance to Potato virus Y, Potato virus S and Potato leafroll virus, respectively, on the basis of previously published and newly modified markers. The sequence characterized amplified region (SCAR) markers for Ryadg, Ns and PLRV1 and the newly modified cleaved amplified polymorphic sequences (CAPS) marker for Ry-fsto were amplified in one PCR reaction which could simply characterize Ryadg and PLRV.1 resistance. Additional digestion of amplicons with EcoRV and MfeI for genotyping the Ry-fsto and Ns resistance genes, respectively, was needed. The effectiveness of genotyping in triplex and tetraplex PCRs was tested on 35 potato varieties used for potato seed production and breeding programs.  

scholarly journals Molecular Characterization of Recombinant Strains of Potato virus Y From Saudi Arabia

Plant Disease ◽  
2016 ◽  
Vol 100 (2) ◽  
pp. 292-297 ◽  
Author(s):  
Mohamad Chikh-Ali ◽  
Hayam Alruwaili ◽  
Dalton Vander Pol ◽  
Alexander V. Karasev
Keyword(s):  
United States ◽  
Saudi Arabia ◽  
Seed Potato ◽  
Potato Virus ◽  
Potato Virus Y ◽  
The United States ◽  
Tuber Quality ◽  
Rt Pcr ◽  
First Report ◽  
Recombinant Strains

Potato virus Y (PVY) exists as a complex of strains, many of which are recombinants. The practical importance of PVY recombinant strains has increased due to their ability to induce potato tuber necrotic ring spot disease (PTNRD) that seriously affects tuber quality. In Saudi Arabia, potato production has increased fivefold during the last three decades, reaching 460,000 tons per year. Although PVY has been reported as one of the main viruses affecting potatoes, no information is available on PVY strains circulating in the country. In August 2014, a survey was conducted in a seed potato field at Al-Jouf, Saudi Arabia. PVY-positive samples selected based on visual symptoms and serological reactivity were subjected to strain typing using multiplex RT-PCR assays and were determined to represent recombinant PVY strains. Whole genome sequences were determined for two representative isolates, S2 and S9, through direct sequencing of a series of overlapping RT-PCR fragments for each isolate, and found to represent strains PVY-NE11 and PVYZ (SYR-III), respectively. One of the recombinant types, SYR-III, was previously found in nearby Syria and Jordan, but the second recombinant, PVY-NE11, was found before only in the United States. Both recombinants, PVY-NE11 and SYR-III, were previously found associated with PTNRD and thought to be rare. The current identification of PVY-NE11 and SYR-III in seed potato in a new geographic region suggests that these recombinants may not be as rare as previously believed. This is the first report on the occurrence of recombinant strains of PVY in potato in Saudi Arabia, and the first report on the PVY-NE11 strain of PVY found in potato outside of the United States.

scholarly journals PHYTOVIRUS MONITORING AT THE POTATO FIELDS IN CHERNIHIV REGION AGROCENOSES

2016 ◽  
Vol 23 ◽  
pp. 36-41
Author(s):  
O. O. Dmitruk ◽  
S. V. Derevianko ◽  
L. M. Reshotko ◽  
I. V. Volkova ◽  
О. О. Kucheriavenko ◽  
...  
Keyword(s):  
Species Composition ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Viral Disease ◽  
Climatic Conditions ◽  
Viral Diseases ◽  
Potato Seed ◽  
Virus Diseases ◽  
Safeguard Measures ◽  
Infection Pathways

The results of monitoring researches of potato virus diseases in the soil and climatic conditions of the Chernigov region. An analysis of the situation in the potato seed shows that the variety is largely defeat viral diseases. In potato seed plots found: potato M-, S-, Y-virus, in monoinfection, and as part of pathocomplexes. Prevails in crops entomophilous potato M virus as monoinfection (36 %) or in combination with other mosaic viruses. Potato virus Y is found in 18 from 47 potato cultivars. A study of the species composition of pathogens and their vectors, infection pathways in the field and deve lopment of viral disease, is the basis for the proper and effective application of safeguard measures.

scholarly journals Genetic Diversity of the Ordinary Strain of Potato virus Y (PVY) and Origin of Recombinant PVY Strains

2011 ◽  
Vol 101 (7) ◽  
pp. 778-785 ◽  
Author(s):  
Alexander V. Karasev ◽  
Xiaojun Hu ◽  
Celeste J. Brown ◽  
Camille Kerlan ◽  
Olga V. Nikolaeva ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Tree ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Molecular Study ◽  
The United States ◽  
Whole Genome ◽  
Whole Genome Analysis ◽  
Severe Stunting ◽  
Ordinary Strain

The ordinary strain of Potato virus Y (PVY), PVYO, causes mild mosaic in tobacco and induces necrosis and severe stunting in potato cultivars carrying the Ny gene. A novel substrain of PVYO was recently reported, PVYO-O5, which is spreading in the United States and is distinguished from other PVYO isolates serologically (i.e., reacting to the otherwise PVYN-specific monoclonal antibody 1F5). To characterize this new PVYO-O5 subgroup and address possible reasons for its continued spread, we conducted a molecular study of PVYO and PVYO-O5 isolates from a North American collection of PVY through whole-genome sequencing and phylogenetic analysis. In all, 44 PVYO isolates were sequenced, including 31 from the previously defined PVYO-O5 group, and subjected to whole-genome analysis. PVYO-O5 isolates formed a separate lineage within the PVYO genome cluster in the whole-genome phylogenetic tree and represented a novel evolutionary lineage of PVY from potato. On the other hand, the PVYO sequences separated into at least two distinct lineages on the whole-genome phylogenetic tree. To shed light on the origin of the three most common PVY recombinants, a more detailed phylogenetic analysis of a sequence fragment, nucleotides 2,406 to 5,821, that is present in all recombinant and nonrecombinant PVYO genomes was conducted. The analysis revealed that PVYN:O and PVYN-Wi recombinants acquired their PVYO segments from two separate PVYO lineages, whereas the PVYNTN recombinant acquired its PVYO segment from the same lineage as PVYN:O. These data suggest that PVYN:O and PVYN-Wi recombinants originated from two separate recombination events involving two different PVYO parental genomes, whereas the PVYNTN recombinants likely originated from the PVYN:O genome via additional recombination events.

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