scholarly journals Reevaluation of Host Specificity of the Closely Related Species Pseudoperonospora humuli and P. cubensis

Plant Disease ◽  
2012 ◽  
Vol 96 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Fabian Runge ◽  
Marco Thines

Pseudoperonospora cubensis causes some of the most devastating diseases of cucurbitaceous crops, while P. humuli is an important pathogen of hop (Cannabaceae). Although parasitic to different Angiosperm orders, these pathogens are highly similar, both in morphology and based on molecular comparisons. Considering the close relationship of P. humuli and P. cubensis, it was hypothesized that cross infectivity of the pathogens between their optimum hosts might be possible. Two strains of P. humuli and one of P. cubensis used in this study were able to reproduce on the two wild cucurbit relatives Bryonia dioica and Sicyos angulatus. Interestingly, limited infectivity of the P. cubensis strain to hop was also observed, and the P. humuli strain was also able to infest Cucumis sativus. The cross infections were verified by comparing the nuclear ribosomal-internal transcribed spacer sequences of newly produced sporangiophores from the different hosts. Morphologically the two pathogens could be distinguished by statistical analyses on their original hosts but these differences diminished on some alternate hosts. The ability of P. cubensis to infest the perennial Bryonia dioica and Humulus lupulus might offer a limited possibility for overwintering in temperate regions and needs to be evaluated in future studies. The ability of P. humuli to complete its life cycle on all the tested cucurbits provides evidence that the host jump across orders may continue and points to the high virulence potential of this pathogen.

Nematology ◽  
2020 ◽  
Vol 22 (8) ◽  
pp. 867-877
Author(s):  
Milad Rashidifard ◽  
Tesleem T. Bello ◽  
Hendrika Fourie ◽  
Danny L. Coyne ◽  
Reyes Peña-Santiago

Summary A new species of Aporcelaimellus, collected in a watermelon field in Nigeria, is described, including its morphological and molecular (D2-D3 28S-rDNA, 18r-DNA) characterisation. Aporcelaimellus nigeriensis sp. n. is distinguishable by its 2.76-3.55 mm length, very coarse ventral body pores, lip region offset by deep constriction and 24-27 μm broad odontostyle 30-36 μm long at its dorsal and 28-31 μm at its ventral side, neck 648-779 μm long, pharyngeal expansion occupying 54-60% of total neck length, uterus 300-473 μm or 2.1-3.2 body diam. long and tripartite, V = 49-54, tail short and convex conoid (27-41 μm, c = 72-115, c′ = 0.5-0.7), spicules 108-137 μm long, and 9-10 spaced ventromedian supplements with hiatus. LSU analysis revealed a close relationship of A. nigeriensis sp. n. with other Aporcelaimellus species and questioned, once more, the monophyly of Aporcelaimidae. SSU phylogenetic tree was not able to resolve the relationship between the new species and other closely related species.


2013 ◽  
Vol 25 (5) ◽  
pp. 648-656 ◽  
Author(s):  
Daniel Remias ◽  
Hans Wastian ◽  
Cornelius Lütz ◽  
Thomas Leya

AbstractIn Antarctica, mass accumulations of psychrophilic algae cause striking phenomena like green, orange, or red snow. This occurs during summer, when coastal snowfields start to melt, become waterlogged and photoautotrophs can thrive. Chloromonas polyptera (Fritsch) Hoham, Mullet & Roemer is a unicellular species that causes orange snow in the vicinity of penguin rockeries. It has been recognized for many decades because of the distinct habitat and the characteristic morphology of cysts with elongated flanges on the outer cell wall. However, closer investigations concerning the ecology or physiology have been sparse so far. Field material was collected from two sites on the Antarctic Peninsula to find out more about metabolic and cellular strategies. The results were compared with a closely related species from high alpine locations, Chloromonas nivalis (Chodat) Hoham & Mullet. Despite the geographical distance, C. polyptera shares several physiological strategies with the alpine relative, such as the formation of cyst stages, saccharose and glycerol as main soluble carbohydrates and the abundant accumulation of the carotenoid astaxanthin. Moreover, photosynthesis is adapted to temperatures of about 1°C. The molecular phylogeny confirmed a close relationship of C. polyptera to other Chloromonas species isolated from snow. Chloromonas polyptera seems to be exclusive to coastal Antarctic ecosystems influenced by animal nutrient input.


1938 ◽  
Vol 70 (11) ◽  
pp. 230-232 ◽  
Author(s):  
C. Stuart Walley

The following notes were assembled in arranging the Protarchoides material in the National Collection. In establishing the identity of Protarchoides mellipes (Prov.) it has been found necessary to synonymize one species. A species allied to mellipes is described as new and a table is provided for the separation of the four known North American species. The recording of Trichiosoma as host for a member of this genus is further evidence of the close relationship of the genus with Protarchus Foer.


2007 ◽  
Vol 44 (3) ◽  
pp. 120-125 ◽  
Author(s):  
B. Zheng ◽  
L. Xiao ◽  
X. Wang ◽  
D. Li ◽  
Y. Lu ◽  
...  

AbstractTo study the function of the 49 kDa excretory-secretory (ES) protein gene (P49) of Trichinella, the genes was amplified by RT-PCR from RNA of Trichinella spiralis and Trichinella nativa and several Chinese Trichinella isolates of domestic animals, and sequenced after being cloned. The amplified products of these parasites produced bands of about 950 bp. The 97.2 % to 100 % nucleotides identity and 94.3 % to 100 % identity of deduced amino acids among P49 gene of these Trichinella strains showed the close relationship of these parasites. The P49 gene of T. nativa was cloned into the BamHI site of the prokaryotic expression vector pET-30a, and the recombinant vector was expressed. The expressed product was 40.8 kDa in size. In Western blot analysis, the expressed product was reactive to sera of mice infected with T. nativa, T. spiralis and their Chinese geographical strains.


2021 ◽  
Vol 37 (2) ◽  
pp. 3-19
Author(s):  
A.A. Gagarina

The present review describes the concept of probiotics for plants and analyzes the prospects for using actinomycetes as producers of these drugs. The minimum requirements for plant probiotic microorganisms are proposed, similar to those for human probiotic microorganisms. These are utility, efficiency and safety for plants, as well as mandatory isolation from plant samples. It is noted that these requirements are usually met by endophytic and rhizosphere microorganisms that stimulate plant growth and provide them with protection from phytopathogens. Evidence is given for the possibility of attributing actinomycetes to probiotic plant bacteria, due to the close relationship of these microorganisms with plants, their wide distribution in populations of endophytic and rhizosphere microorganisms, and the presence of phytoregulatory activity. The review provides examples of genera and species of actinomycetes that are promising producers of probiotics for agronomically important crops. The most studied and commercialized of them are representatives of the Streptomyces genus. The current state, prospects and problems in commercialization of probiotics based on actinomycetes are discussed. probiotic microorganisms of plants, associative actinomycetes, endophytes, rhizosphere, biological preparations


Author(s):  
KAMARUL AFENDEY BIN HAMIMI ◽  
AHMAD ZULLAILI BIN ZAMRI

Kertas kerja ini menganalisis peranan Ustaz Abu Bakar al-Baqir iaitu lama reformis tempatan yang konsisten menentang British sehingga terpenjara pada 1948. Beliau turut menubuhkan Maahad al-Ehya Assyariff untuk memodenkan sistem pendidikan pondok di samping meniupkan semangat kemerdekaan kepada pelajar dan masyarakat di sekelilingnya. Objektif kajian ini adalah untuk mengetengahkan sumbangan Ustaz Abu Bakar al-Baqir sebagai seorang ulama dan pejuang kemerdekaan menentang British sekitar 1934 sehingga 1957 di Perak. Kajian ini berbentuk historiografi yang menggunakan kaedah kualitatif dengan pendekatan analisis kandungan. Dua jenis sumber digunakan dalam kajian ini iaitu sumber primer dan sekunder. Sumber primer adalah seperti mendapatkan dokumen dan fail di Arkib Negara Malaysia, sumber pejabat kolonial British di samping menemu bual ahli keluarga dan murid Ustaz Abu Bakar al-Baqir. Sumber sekunder pula diperoleh melalui buku, surat khabar dan kertas kerja seminar yang berkaitan dengan perjuangan beliau. Hasil kajian mendapati para pelajarnya terdedah dengan semangat kemerdekaan ini ekoran guru-guru reformis dari Indonesia yang terdedah dengan perjuangan menentang Belanda mengajar di Maahad al-Ehya Assyariff. Selain itu, hubungan baik Ustaz Abu Bakar al-Baqir dengan Ibrahim Yaakub selaku penggerak Kesatuan Melayu Muda (KMM) dan Dr. Burhanuddin al-Helmy yang memimpin Parti Kebangsaan Melayu Muda (PKMM) menjadikan Maahad al-Ehya Assyariff sebagai markas politik menentang British sehingga tertubuhnya Parti Hizbul Muslimin di bawah pimpinan Ustaz Abu Bakar al-Baqir. Kata kunci: Maahad al-Ehya Assyariff, Hizbul Muslimin, British, Kaum Muda, Parti Kebangsaan Melayu Malaya (PKMM), Kesatuan Melayu Muda (KMM). This paper attempts to analyze the role played by Ustaz Abu Bakar al-Baqir, a local reformist religious leader who consistently opposed the British until he was imprisoned by them in 1948. He established the Maahad al-Ehya Assyariff with the aim of modernizing the religious education system besides stoking the spirit of freedom among his students and the society around him. The objective of this study is to highlight the contributions of Ustaz Abu Bakar al-Baqir as a religious leader as well as a freedom fighter who opposed British rule in Perak from around 1934 until 1957. This is a historiographical research which uses qualitative methodology with a content analysis approach. Two different sources of information are used for this study namely primary and secondary sources. Primary sources include documents and files from the Malaysian National Archives and official documents from the British Colonial office, besides interviewing family members as well as former students of Ustaz Abu Bakar al-Baqir. On the other hand, secondary sources include books, newspapers and seminar report relating to his struggle. The findings of the study show that his students were exposed to the struggle for Independence through the direct influence of the reformist teachers from Indonesia who were teaching at the Maahad al-Ehya Assyariff. These teachers had already been involved in the fight against the Dutch colonialists in Indonesia. In addition to this, the close relationship of Ustaz Abu Bakar al-Baqir with Ibrahim Yaakub, the primemover of Kesatuan Melayu Muda (KMM) and Dr. Burhanuddin al-Helmy, the leader of Parti Kebangsaan Melayu Muda (PKMM), projected Maahadal-Ehya Assyariff as the political front that opposed British rule until the founding of the Parti Hizbul Muslimin under the leadership of Ustaz Abu Bakar al-Baqir. Keywords: Maahad al-Ehya Assyariff, Hizbul Muslimin, British, Kaum Muda, Malay Nationalist Party (MNP), Young Malays Union.


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