scholarly journals A New Postharvest Fruit Rot in Apple and Pear Caused by Phacidium lacerum

Plant Disease ◽  
2016 ◽  
Vol 100 (1) ◽  
pp. 32-39 ◽  
Author(s):  
M. S. Wiseman ◽  
Y. K. Kim ◽  
F. M. Dugan ◽  
J. D. Rogers ◽  
C. L. Xiao
Keyword(s):  
Phylogenetic Analysis ◽  
Ribosomal Subunit ◽  
Morphological Characteristics ◽  
Apple Fruit ◽  
Fruit Rot ◽  
Morphological Data ◽  
Postharvest Diseases ◽  
Pear Fruit ◽  
Diseased Fruit ◽  
Molecular Phylogenetic

During surveys for postharvest diseases of apple and pear, an unknown postharvest fruit rot was observed in Washington State. The disease appeared to originate from infection of the stem and calyx tissue of the fruit or wounds on the fruit. An unknown pycnidial fungus was consistently isolated from the decayed fruit. Isolates from apple and pear were characterized and identified by molecular phylogenetic analysis and morphology. Pathogenicity of representative isolates on apple and pear fruit was tested under laboratory or field conditions. A BLAST search in GenBank showed that isolates differed from Phacidium lacerum and its synonym, Ceuthospora pinastri, by only 0 to 4 bp in sequences within part of the combined large ribosomal subunit + internal transcribed spacer + small ribosomal subunit regions. The phylogenetic analysis confirmed the taxonomic placement of the unknown fungus in the genus Phacidium, with the highest match being C. pinastri (formerly anamorphic P. lacerum) and with closely related taxa from GenBank forming congeneric clades. The fungus grew at 0 to 30°C and formed unilocular to multilocular pycnidial conidiomata on artificial media after approximately 5 to 7 days at room temperature. On potato dextrose agar incubated for a 12-h photoperiod, semi-immersed globose to subglobose pycnidial conidiomata were 250 to 1,000 μm in diameter (mean = 350), with 1 to 3 nonpapillate to slightly papillate ostioles and a buff conidial matrix. Conidia produced on phialides were 8 to 13 by 1.5 to 2.5 μm, hyaline, aseptate, cylindrical, with an abruptly tapered, typically slightly protuberant base, 2 to 3 guttules, and sometimes with a mucilaginous, flexuous, unbranched appendage which is attached to the apex of the conidium and disappears with age. Conidiogenous cells were flask shaped and 6 to 15 ×1.5 to 3 μm. Colony characteristics included felt-like aerial white mycelium, gray olivaceous at the center becoming greenish to colorless toward the margin, in concentric rings, with pycnidia forming in 5 to 7 days originating from the center of the plate. Morphological characteristics of the fungus had the greatest conformity with the description for C. pinastri. Based on molecular and morphological data, the fungus is identified as P. lacerum. ‘Fuji’ apple fruit and ‘d’Anjou’ pear fruit that were wounded, inoculated with representative isolates, and incubated at 0°C yielded the same symptoms as seen on decayed fruit collected from commercial fruit packinghouses. Stem-end rot, calyx-end rot, and wound-associated rot developed on fruit inoculated in the orchard after 3 months of cold storage. The fungus was reisolated from the diseased fruit. This is the first report of a fruit rot in apple and pear caused by P. lacerum. We propose Phacidium rot as the name of this disease.

scholarly journals A Postharvest Fruit Rot of Apple Caused by Lambertella corni-maris in Washington State

Plant Disease ◽  
2015 ◽  
Vol 99 (2) ◽  
pp. 201-206 ◽  
Author(s):  
M. S. Wiseman ◽  
F. M. Dugan ◽  
Y. K. Kim ◽  
C. L. Xiao
Keyword(s):  
Ribosomal Subunit ◽  
Morphological Characteristics ◽  
Aerial Mycelium ◽  
The United States ◽  
Skin Lesions ◽  
Apple Fruit ◽  
Washington State ◽  
Fruit Rot ◽  
Postharvest Diseases ◽  
Diseased Fruit

During surveys for postharvest diseases of apple conducted in Washington State, an unknown fruit rot was observed on stored apple fruit collected from commercial fruit packinghouses. This disease was present in 66 of the 179 grower lots sampled, accounting for an average 1 to 3% of the total decayed fruit sampled. The disease appeared to originate from infection of wounds on the fruit skin. Lesions were brown and decayed tissues were spongy. A Lambertella sp. was consistently isolated from the decayed fruit. Sequences of the fungus and those of Lambertella corni-maris in GenBank differed by 0 to 4 bp across the combined small ribosomal subunit + internal transcribed spacer + large ribosomal subunit regions with a maximum identity ranging from 99 to 100%. The fungus grew at 0 to 20°C and formed apothecia on artificial media after 8 to 24 weeks. On potato dextrose agar under a 12-h photoperiod, apothecial dimensions were variable, ranging from 1 to 6 mm in diameter with stipes of 1 to 4 by 0.5 mm. Asci were 76 to 125 by 3.5 to 5.5 μm, inoperculate, eight-spored, clavate, and narrowed at the base. Ascospores were aseptate, 7 to 10 by 2.5 to 4.5 μm, uniseriate to biseriate, and orange-brown at maturity in the ascus. Colony characteristics included little or no aerial mycelium, dark-yellow to gray-black mycelium, gray-black pseudosclerotia, and yellow pigmentation in the agar. Morphological characteristics of the fungus overlapped with the description of L. corni-maris. ‘Fuji’ apple fruit that were wounded, inoculated with representative isolates, and incubated at 0°C yielded the same symptoms as seen in packinghouses, and the fungus was reisolated from the diseased fruit. This is the first report of a fruit rot in stored apple caused by L. corni-maris in the United States. We propose Lambertella rot as the name of this disease.

Phylogenetic, Morphological, and Pathogenic Characterization of Alternaria Species Associated with Fruit rot of Mandarin in California

Plant Disease ◽  
2020 ◽  
Author(s):  
Fei Wang ◽  
Seiya Saito ◽  
Themis Michailides ◽  
Chang-Lin Xiao
Keyword(s):  
Phylogenetic Analysis ◽  
Rna Polymerase Ii ◽  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Central Valley ◽  
Fruit Rot ◽  
Postharvest Diseases ◽  
Plasma Membrane Atpase ◽  
Alternaria Species

Alternaria rot caused by Alternaria species is one of the major postharvest diseases of mandarin fruit in California. The aims of this study were to identify these Alternaria species using phylogenetic analyses and morphological characteristics and test their pathogenicity to mandarin. Decayed mandarin fruit exhibiting Alternaria rot symptoms were collected from three citrus fruit packinghouses in the Central Valley of California. In total, 177 Alternaria isolates were obtained from decayed fruit and preliminarily separated into three groups representing three species (A. alternata, A. tenuissima and A. arborescens) based on the colony characterization and sporulation patterns. To further identify these isolates, phylogenetic analysis was conducted based on DNA sequences of the second largest subunit of RNA polymerase II (RPB2), plasma membrane ATPase (ATPase) and Calmodulin gene regions in combination with morphological characters. Of the 177 isolates, 124 isolates (70.1%) were identified as A. alternata and 53 isolates (29.9 %) were A. arborescens. The isolates initially identified as A. tenuissima based on the morphological characteristics could not be separated from those of A. alternata in phylogenetic analysis and thus considered A. alternata. Pathogenicity tests showed that both Alternaria species were pathogenic on mandarin fruit at both 5°C and 20°C. Our results indicated that two Alternaria species, A. alternata and A. arborescens, were responsible for Alternaria rot of mandarin fruit in California with A. arborescens causing fruit rot on mandarin being reported for the first time.

scholarly journals Identification of Lasiodiplodia pseudotheobromae Causing Fruit Rot of Citrus in China

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 202
Author(s):  
Jianghua Chen ◽  
Zihang Zhu ◽  
Yanping Fu ◽  
Jiasen Cheng ◽  
Jiatao Xie ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Citrus Fruit ◽  
Economic Loss ◽  
Fruit Rot ◽  
Post Inoculation ◽  
Citrus Reticulata ◽  
Postharvest Diseases ◽  
Citrus Reticulata Blanco

Considering the huge economic loss caused by postharvest diseases, the identification and prevention of citrus postharvest diseases is vital to the citrus industry. In 2018, 16 decayed citrus fruit from four citrus varieties—Satsuma mandarin (Citrus unshiu), Ponkan (Citrus reticulata Blanco cv. Ponkan), Nanfeng mandarin (Citrus reticulata cv. nanfengmiju), and Sugar orange (Citrus reticulata Blanco)—showing soft rot and sogginess on their surfaces and covered with white mycelia were collected from storage rooms in seven provinces. The pathogens were isolated and the pathogenicity of the isolates was tested. The fungal strains were identified as Lasiodiplodia pseudotheobromae based on their morphological characteristics and phylogenetic analyses using the internal transcribed spacer regions (ITS), translation elongation factor 1-α gene (TEF), and beta-tubulin (TUB) gene sequences. The strains could infect wounded citrus fruit and cause decay within two days post inoculation, but could not infect unwounded fruit. To our knowledge, this is the first report of citrus fruit decay caused by L. pseudotheobromae in China.

scholarly journals Primula sunhangii (Primulaceae): a new species from Hubei, Central China

PhytoKeys ◽  
2020 ◽  
Vol 156 ◽  
pp. 103-112
Author(s):  
Jiao Sun ◽  
Dai-Gui Zhang ◽  
Xian-Han Huang ◽  
Komiljon Tojibaev ◽  
Jing-Yuan Yang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Hubei Province ◽  
Central China ◽  
Morphological Data ◽  
Molecular Phylogenetic Analysis ◽  
Molecular Phylogenetic ◽  
A New Species

This report provides a description of Primula sunhangii from the Shennongjia Forestry District, Hubei Province in Central China, which is categorized as a new species of the primrose family. Primula sunhangii is morphologically similar to P. involucrata Wall. ex Duby in terms of its simple umbel, efarinose, and prolonged bracts. However, P. sunhangii is distinguished by its glabrous sepal, short petiole (compare with blade) and cylindrical calyx and capsule. Molecular phylogenetic analysis based on nuclear and cpDNA genes demonstrates that P. sunhangii and P. involucrata are closely related. Combining genetic and morphological data, the recognition of P. sunhangii as a unique new species is supported.

scholarly journals Two newly recorded invasive alien ascidians (Chordata, Tunicata, Ascidiacea) based on morphological and molecular phylogenetic analysis in Korea

Zootaxa ◽  
2012 ◽  
Vol 3368 (1) ◽  
pp. 211 ◽  
Author(s):  
JOOYEON PYO ◽  
TAEKJUN LEE ◽  
SOOK SHIN
Keyword(s):  
Invasive Species ◽  
Phylogenetic Analysis ◽  
Molecular Analysis ◽  
Phylogenetic Relationships ◽  
18S Rdna ◽  
Morphological Characteristics ◽  
Korea Strait ◽  
Molecular Phylogenetic ◽  
Distinct Features ◽  
First Time

We report five alien ascidians with some distinct features that were investigated from August 2009 to October 2011 in Korea, among which Ascidiella aspersa (Müller, 1776) belonging to family Ascidiidae of order Phlebobranchia and Molgula manhattensis (De Kay, 1843) belonging to family Molgulidae of order Stolidobranchia are newly discovered invasive species. These ascidians were identified and their phylogenetic relationships were clarified through molecular analysis using about 680 bp of nuclear 18S rDNA and about 670 bp of mt-COI genes along with detailed morphological characteristics, and reported for the first time in Korea. It was discovered that A. aspersa was widespread three coastlines of Korea except Jeju Island, and M. manhattensis first found in Mokpo, Gunsan, and Incheon in June 2010 extended into Busan of Korea Strait in 2011.

Polyphasic reassessment of a yellow rice fungus, Penicillium toxicarium

Nova Hedwigia ◽  
2020 ◽  
Vol 111 (3) ◽  
pp. 459-472
Author(s):  
Yoshitsugu Sugiura ◽  
Maiko Watanabe ◽  
Naoki Kobayashi
Keyword(s):  
Phylogenetic Analysis ◽  
Short Communication ◽  
Morphological Characteristics ◽  
Taxonomic Position ◽  
Molecular Phylogenetic Analysis ◽  
Latin Diagnosis ◽  
Molecular Phylogenetic ◽  
Toxigenic Fungus ◽  
Unique Clade ◽  
Latin Description

From historical strain records, Penicillium citreonigrum NBRC 4692 was originally isolated as the toxigenic fungus responsible for the yellow rice incident in Japan in 1937. The fungus was named Penicillium toxicarium by I. Miyake, which was considered invalid due to the lack of a Latin diagnosis. Initially, it was named Penicillium sp. by Miyake et al. in 1940. Subsequently, P. toxicarium, which was erroneously cited as synonym of P. citreo-viride, was validated by C. Ramírez in 1982 with a Latin diagnosis and type designation (CBS 351.51). Later, CBS 351.51 was assigned to Penicillium trzebinskii by Houbraken et al. in 2014. In 2016, P. toxicarium was treated as a synonym of Penicillium citreosulfuratum based on a conclusion of molecular phylogenetic analysis. Recently, we discovered the taxonomic and nomenclatural short communication (in Japanese) by I. Miyake in 1947 on P. toxicarium sp. nov. with its Latin description and four illustrations but lacking the type designation. In this paper, we re-examined strain NBRC 4692 for its current taxonomic position based on morphological characteristics and molecular phylogenetic analysis. NBRC 4692 (received from I. Miyake in 1951) has been found to have the same morphological characteristics as P. toxicarium, as was shown in Miyake's diagnosis in 1947. Phylogenetic analysis revealed that the NBRC strain belongs to a unique clade, different from the clade comprising P. citreosulfuratum strains. As a conclusion, herein, P. toxicarium I. Miyake (1947) is reinstated as a correct name with the lectotype designation.

Two new species of Tomentella (Thelephorales, Basidiomycota) from Lesser Xingan Mts., northeastern China

Phytotaxa ◽  
2018 ◽  
Vol 369 (2) ◽  
pp. 80 ◽  
Author(s):  
XU LU ◽  
YAN-HONG MU ◽  
HAI-SHENG YUAN
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Maximum Likelihood ◽  
Morphological Characteristics ◽  
Northeastern China ◽  
Phylogenetic Position ◽  
Closely Related Species ◽  
Molecular Analyses ◽  
Two New Species ◽  
Molecular Phylogenetic

Two new species of Tomentella from the Lesser Xingan Mts. of northeastern China, T. pallidocastanea and T. tenuirhizomorpha, are described and illustrated using morphological characteristics and molecular phylogenetic analysis. T. pallidocastanea is characterized by mucedinoid basidiocarps adherent to the substrate, a light reddish brown to pinkish grey hymenophoral surface, absence of rhizomorphs and cystidia, generative hyphae with clamps, rarely with simple septa and echinulate, and subglobose to lobed basidiospores (echinuli up to 1.5 μm long). T. tenuirhizomorpha is characterized by mucedinoid basidiocarps separable from the substrate, a greyish brown to dark brown hymenophoral surface, thin rhizomorphs in the subiculum and margins, an absence of cystidia, generative hyphae with clamps rarely with simple septa and echinulate, and subglobose to globose basidiospores (echinuli up to 1.5 μm long). Molecular analyses using Maximum Likelihood, Maximum Parsimony and Bayesian Analysis confirm the phylogenetic position of the two new species. The discriminating characters of these two new species and their closely related species are discussed in this study.

scholarly journals A Postharvest Fruit Rot in d'Anjou Pears Caused by Sphaeropsis pyriputrescens sp. nov

Plant Disease ◽  
2004 ◽  
Vol 88 (2) ◽  
pp. 114-118 ◽  
Author(s):  
C. L. Xiao ◽  
J. D. Rogers
Keyword(s):  
Storage Period ◽  
Washington State ◽  
Fruit Rot ◽  
Postharvest Diseases ◽  
Pear Fruit ◽  
Oatmeal Agar ◽  
Colony Diameter ◽  
Light Yellow ◽  
Fruit Decay ◽  
Pathogenicity Tests

During a survey of postharvest diseases in stored pears conducted in the 2001-02 storage season, a new postharvest fruit rot in d'Anjou pears was discovered in Washington State. Symptoms of this disease were stem-end rot, calyx-end rot, and wound-associated rot, which presumably originated from infections of stem, calyx, and wounds on the fruit surfaces, respectively. The decayed area on the fruit was firm or spongy and appeared brown. During the late storage period from March to May 2002, this disease was observed in 19 of 39 lots and accounted for 2 to 21% of all decayed fruit. The causal agent, Sphaeropsis sp., was consistently recovered from decayed fruit with the symptoms described above. Two isolates of the fungus were used for pathogenicity tests on pear fruit. Decay symptoms developed on fruit inoculated with spore suspensions of the fungus on the stem, calyx, and wounds on the fruit surface. The fungus was reisolated from these decayed fruit. The fungus, Sphaeropsis pyriputrescens sp. nov., was characterized and described. On potato dextrose agar (PDA), oatmeal agar, and pear juice agar at 20°C, the fungus grew at mean rates of 21, 15, and 24 mm day-1 in colony diameter, respectively. On PDA, the fungus formed a circular colony with dense, hyaline hyphae and a few or some aerial mycelia. Colonies appeared light yellow to yellow on 2-week-old PDA cultures. The fungus grew at temperatures from 0 to 25°C, with optimum growth between 15 and 20°C, little or no growth at 30°C, and no growth at 35°C. This is a low-temperature species.

A morphological and molecular phylogenetic analysis of relationships between genera of the nematode sub-family Cloacininae (Stossich) (Strongyloidea: Chabertiidae) parasitic in kangaroos, wallabies and rat-kangaroos (Marsupialia: Macropodoidea) 

Zootaxa ◽  
2020 ◽  
Vol 4851 (2) ◽  
pp. 271-288
Author(s):  
I. BEVERIDGE ◽  
A. JABBAR ◽  
A. KOEHLER ◽  
T. SUKEE
Keyword(s):  
Phylogenetic Analysis ◽  
Ribosomal Dna ◽  
Morphological Characteristics ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Molecular Phylogenetic Analysis ◽  
Molecular Phylogenetic ◽  
Phenetic Classification

A phylogenetic analysis of the genera of the strongyloid sub-family Cloacininae from macropodoid marsupials in Australasia was undertaken based on morphological characteristics and analysis of concatenated sequences (ITS+) of the first (ITS-1) and second (ITS-2) internal transcribed spacers of nuclear ribosomal DNA. Neither approach provided a robust phylogeny, but similarities between the two methods in terms of generic groupings suggested that substantial revision is needed of the current phenetic classification, with some of the key morphological characteristics currently used to define genera and tribes proving to be homoplasious. 

scholarly journals Fungi Causing Storage Rot of Apple Fruit in Integrated Pest Management System and their Sensitivity to Fungicides

2015 ◽  
Vol 34 (329) ◽  
pp. 2-11 ◽  
Author(s):  
Lelde Grantina-Ievina
Keyword(s):  
Secondary Infection ◽  
Morphological Characteristics ◽  
Storage Period ◽  
Fungal Species ◽  
Apple Fruit ◽  
Fruit Rot ◽  
Penicillium Expansum ◽  
Northern Europe ◽  
Its2 Region ◽  
Fruit Storage

Abstract Apple fruit rot can be caused by several fungi. In Northern Europe, the most common storage rot, Bull’s eye rot, is caused by Neofabraea spp., bitter rot by Colletotrichum spp., brown rot by Monilinia fructigena, grey mould is caused by Botrytis cinerea and Fusarium rot by several Fusarium species. Blue mold decay caused by Penicillium expansum is an important disease in several European countries. Incidence of different causal agents may vary depending on cultivar, climate during growing season and agricultural practices. The main objective of the study was to obtain baseline information about apple rot-causing fungi, their incidence during fruit storage and to evaluate the fungicide sensitivity of most of isolated fungal species. The study was performed during the storage period of apples after the growth season of 2013. Rotten apples were sorted in the storage and part of them was brought to the laboratory in order to obtain fungal isolates. Fungi were identified according to the morphological characteristics and sequencing of the ITS1-5.8S-ITS2 region. During storage in February and March the total percentage of rotten apples in various cultivars varied from 3.6 to 58.9%. All post-harvest diseases described in Northern Europe were detected. In part of the storehouses apple rot caused by Cadophora luteo-olivacea was observed. Alternaria spp. and Cladosporium spp. were detected on few apples as secondary infection agents. Using the most often isolated fungal species, sensitivity tests were performed against five commonly used fungicides. In general, the sensitivity of tested fungi to the fungicides was high with exception of several Neofabraea and Alternaria isolates.

Sign in / Sign up

Export Citation Format

Share Document