scholarly journals Scarification and Environmental Factors that Enhance Carpogenic Germination of Sclerotia of Sclerotinia sclerotiorum

Plant Disease ◽  
2010 ◽  
Vol 94 (8) ◽  
pp. 1041-1047 ◽  
Author(s):  
Harsh Garg ◽  
Krishnapillai Sivasithamparam ◽  
Martin J. Barbetti
Keyword(s):  
Environmental Factors ◽  
Sclerotinia Sclerotiorum ◽  
Tap Water ◽  
Primary Source ◽  
Sclerotinia Stem Rot ◽  
River Sand ◽  
Carpogenic Germination ◽  
Cropping Season ◽  
Disease Epidemics ◽  
Selection Of

Ascospores of Sclerotinia sclerotiorum are the primary source of inoculum for disease epidemics in many economically important crops. Mass production of ascospores under laboratory conditions is required to prepare inoculum for use in selection of genotypes with resistance against Sclerotinia diseases. A study was undertaken, first, to investigate the effect on carpogenic germination of scarifying sclerotia from two S. sclerotiorum isolates taken from canola (Brassica napus) and, second, to identify environmental factors that enhance carpogenic germination. Seven different environmental treatments were applied to scarified and unscarified sclerotia: (i) sterilized distilled water for 4 months at 15°C, (ii) aerated water for 4 months at 4°C, (iii) constant rinsing with tap water for 8 weeks at 4°C, (iv) cold-conditioning for 4 weeks at 4°C and subsequent transfer into moist unsterilized compost at 15°C, (v) incubation in sterilized river sand at 15°C, (vi) air drying for 2 weeks followed by subsequent transfer into sterilized moist river sand at 15°C, or (vii) placed into 0.5% water agar and incubated at 15°C. Carpogenic germination of scarified sclerotia was significantly greater (P < 0.05) than for unscarified sclerotia. There was significant interaction (P < 0.001) between scarification and the different environmental treatments in relation to the carpogenic germination. Carpogenic germination of scarified sclerotia was enhanced by incubation of sclerotia in compost or in sterilized river sand. Further, overall carpogenic germination of both scarified and unscarified sclerotia occurred to the greatest extent when sclerotia of either of the two isolates were subjected to constant rinsing with tap water. We believe this to be the first report of both the enhanced carpogenic germination by scarification in S. sclerotiorum and the environmental factors we report that enhance carpogenic germination of scarified sclerotia. The progression of carpogenic germination in all the environmental treatments was also monitored as a part of this study across the two consecutive years for the same two isolates. The majority of sclerotia of both isolates germinated between the months of June and September in both years, a period which coincides with the main part of the cropping season when Sclerotinia stem rot is normally observed in rainfed canola in Western Australia. These data suggested the existence of a seasonal rhythm-like pattern in relation to the carpogenic germination of this pathogen.

scholarly journals Favorable Bioactivity of the SDHI Fungicide Benzovindiflupyr Against Sclerotinia sclerotiorum Mycelial Growth, Sclerotial Production, and Myceliogenic and Carpogenic Germination of Sclerotia

Plant Disease ◽  
2019 ◽  
Vol 103 (7) ◽  
pp. 1613-1620 ◽  
Author(s):  
Xue-ping Huang ◽  
Jian Luo ◽  
Yu-fei Song ◽  
Bei-xing Li ◽  
Wei Mu ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Mycelial Growth ◽  
Disease Incidence ◽  
Stem Rot ◽  
Sclerotinia Stem Rot ◽  
Baseline Sensitivity ◽  
Carpogenic Germination ◽  
Alternative Product ◽  
Application Potential ◽  
New Generation

Sclerotinia sclerotiorum, which can cause Sclerotinia stem rot, is a prevalent plant pathogen. This study aims to evaluate the application potential of benzovindiflupyr, a new generation of succinate dehydrogenase inhibitor (SDHI), against S. sclerotiorum. In our study, 181 isolates collected from different crops (including eggplant [n = 34], cucumber [n = 27], tomato [n = 29], pepper [n = 35], pumpkin [n = 32], and kidney bean [n = 25]) in China were used to establish baseline sensitivity to benzovindiflupyr. The frequency distribution of the 50% effective concentration (EC50) values of benzovindiflupyr was a unimodal curve, with mean EC50 values of 0.0260 ± 0.011 μg/ml, and no significant differences in mean EC50 existed among the various crops (P > 0.99). Benzovindiflupyr can effectively inhibit mycelial growth, sclerotial production, sclerotial shape, and myceliogenic and carpogenic germination of the sclerotia of S. sclerotiorum. In addition, benzovindiflupyr showed good systemic translocation in eggplant. Using benzovindiflupyr at 100 μg/ml yielded efficacies of 71.3 and 80.5% for transverse activity and cross-layer activity, respectively, which were higher than those of acropetal and basipetal treatments (43.6 and 44.7%, respectively). Greenhouse experiments were then carried out at two experimental sites for verification. Applying benzovindiflupyr at 200 g a.i. ha−1 significantly reduced the disease incidence and severity of Sclerotinia stem rot. Overall, the results demonstrated that benzovindiflupyr is a potential alternative product to control Sclerotinia stem rot.

scholarly journals Carpogenic Germinability of Diverse Sclerotinia sclerotiorum Populations Within the Southwestern Australian Grain Belt

Plant Disease ◽  
2020 ◽  
Vol 104 (11) ◽  
pp. 2891-2897
Author(s):  
Pippa J. Michael ◽  
King Yin Lui ◽  
Linda L. Thomson ◽  
Katia Stefanova ◽  
Sarita J. Bennett
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Temperature Fluctuations ◽  
Sclerotinia Stem Rot ◽  
Dark Cycle ◽  
Diurnal Temperature ◽  
Population Responses ◽  
Carpogenic Germination ◽  
Southwestern Australia ◽  
Temperature Regimes ◽  
Mycelial Compatibility

Sclerotinia stem rot, caused by the necrotrophic plant pathogen Sclerotinia sclerotiorum (Lib.) de Bary, is a major disease of canola and pulses in Australia. Current disease management relies greatly on cultural and chemical means of control. Timing of fungicide applications remains a challenge, because efficacy is dependent on accurate prediction of ascospore release and presence on the plant. The aims of this study were to determine the optimal temperature for carpogenic germination of S. sclerotiorum populations sampled from canola and lupin fields in southwestern Australia and characterize diversity using mycelial compatibility groupings (MCGs). Sclerotia were collected from four diseased canola and one diseased lupin field from across southwestern Australia. Forty sclerotia from each population were incubated at four alternating temperatures of 30/15, 20/15, 20/4, and 15/4°C (12-h/12-h light/dark cycle) and assessed every 2 to 3 days for a 180-day period. MCG groupings for populations were characterized using 12 reference isolates. Results indicated the time to initial carpogenic germination decreased as diurnal temperature fluctuations decreased, with a fluctuation of 5°C (20/15°C) having the most rapid initial germination followed by 11°C (15/4°C) followed by 16°C (20/4°C). Optimal germination temperature for all five populations was 20/15°C; however, population responses to other diurnal temperature regimes varied considerably. No germination was observed at 30/15°C. MCG results indicate extensive diversity within and between populations, with at least 40% of sclerotia within each population unable to be characterized. We suggest that this diversity has enabled S. sclerotiorum populations to adapt to varying environmental conditions within southwestern Australia.

scholarly journals Longevity of Sclerotinia sclerotiorum sclerotia on the soil surface under field conditions

2016 ◽  
Vol 42 (2) ◽  
pp. 172-174 ◽  
Author(s):  
Ricardo Brustolin ◽  
Erlei Melo Reis ◽  
Lucas Pedron
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Sodium Hypochlorite ◽  
Tap Water ◽  
Soil Surface ◽  
River Sand ◽  
Nylon Mesh ◽  
No Till ◽  
Cleaning Machine ◽  
Soybean Plants ◽  
Commercial Field

ABSTRACT The longevity of Sclerotinia sclerotiorum sclerotia was quantified in an experiment carried out in the field. Sclerotia naturally formed in soybean plants in an infested commercial field were collected in a grain-cleaning machine and those present in the stem pith, with c.a. 8 mm in length and 1.9 mm in diameter were selected. Fifty sclerotia were kept inside a white nylon mesh (0.25mm) screen bag (25 x 25cm). Eighty bags were laid on the soil surface-simulating no till farming. At monthly intervals, four bags were taken and brought to the laboratory. Sclerotia were washed with tap water and surface desinfested with sodium hypochlorite and exposed to germinate on sterilized moist river sand in a growth chamber at 15oC and 12h photoperiod. After 12 months, sclerotia kept on the soil surface, lost their viability. It may be concluded that under no till, crop rotation with nonsusceptible crops, can reduce the sclerotia bank in the soil.

scholarly journals Potential for Aquifer Storage and Recovery (ASR) in South Bihar, India

2021 ◽  
Vol 13 (6) ◽  
pp. 3502
Author(s):  
Somnath Bandyopadhyay ◽  
Aviram Sharma ◽  
Satiprasad Sahoo ◽  
Kishore Dhavala ◽  
Prabhakar Sharma
Keyword(s):  
Pilot Study ◽  
Environmental Sustainability ◽  
Hard Rock ◽  
Climatic Conditions ◽  
Aquifer Storage And Recovery ◽  
Aquifer Recharge ◽  
Aquifer Storage ◽  
Cropping Season ◽  
Selection Of ◽  
Surface Technique

Among the several options of managed aquifer recharge (MAR) techniques, the aquifer storage and recovery (ASR) is a well-known sub-surface technique to replenish depleted aquifers, which is contingent upon the selection of appropriate sites. This paper explores the potential of ASR for groundwater recharge in the hydrological, hydrogeological, social, and economic context of South Bihar in India. Based on the water samples from more than 137 wells and socio-economic surveys, ASR installations were piloted through seven selected entrepreneurial farmers in two villages of South Bihar. The feasibility of ASR in both hard rock and deep alluvial aquifers was demonstrated for the prominent aquifer types in the marginal alluvial plains of South Bihar and elsewhere. It was postulated through this pilot study that a successful spread of ASR in South Bihar can augment usable water resources for agriculture during the winter cropping season. More importantly, ASR can adapt to local circumstances and challenges under changing climatic conditions. The flexible and participatory approach in this pilot study also allowed the farmers to creatively engage with the design and governance aspects of the recharge pit. The entrepreneurial farmers-led model builds local accountability, creates avenues for private investments, and opens up the space for continued innovation in technology and management, while also committing to resource distributive justice and environmental sustainability.

scholarly journals The Winter Habitat Selection of Red Deer (Cervus elaphus) Based on a Multi-Scale Model

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2454
Author(s):  
Yue Sun ◽  
Yanze Yu ◽  
Jinhao Guo ◽  
Minghai Zhang
Keyword(s):  
Habitat Selection ◽  
Environmental Factors ◽  
Cervus Elaphus ◽  
Red Deer ◽  
Scale Model ◽  
Winter Habitat ◽  
Multi Scale ◽  
Optimal Scale ◽  
Scale Models ◽  
Selection Of

Single-scale frameworks are often used to analyze the habitat selections of species. Research on habitat selection can be significantly improved using multi-scale models that enable greater in-depth analyses of the scale dependence between species and specific environmental factors. In this study, the winter habitat selection of red deer in the Gogostaihanwula Nature Reserve, Inner Mongolia, was studied using a multi-scale model. Each selected covariate was included in multi-scale models at their “characteristic scale”, and we used an all subsets approach and model selection framework to assess habitat selection. The results showed that: (1) Univariate logistic regression analysis showed that the response scale of red deer to environmental factors was different among different covariate. The optimal scale of the single covariate was 800–3200 m, slope (SLP), altitude (ELE), and ratio of deciduous broad-leaved forests were 800 m in large scale, except that the farmland ratio was 200 m in fine scale. The optimal scale of road density and grassland ratio is both 1600 m, and the optimal scale of net forest production capacity is 3200 m; (2) distance to forest edges, distance to cement roads, distance to villages, altitude, distance to all road, and slope of the region were the most important factors affecting winter habitat selection. The outcomes of this study indicate that future studies on the effectiveness of habitat selections will benefit from multi-scale models. In addition to increasing interpretive and predictive capabilities, multi-scale habitat selection models enhance our understanding of how species respond to their environments and contribute to the formulation of effective conservation and management strategies for ungulata.

Prevalence of sclerotinia stem rot of canola in New South Wales

2003 ◽  
Vol 43 (2) ◽  
pp. 163 ◽  
Author(s):  
T. L. Hind ◽  
G. J. Ash ◽  
G. M. Murray
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
New South ◽  
New South Wales ◽  
Stem Rot ◽  
Sclerotinia Stem Rot ◽  
Mean Values ◽  
Stem Infection ◽  
South Wales

Surveys of petal infestation and stem infection conducted in 1998, 1999 and 2000 indicated that Sclerotinia sclerotiorum poses a threat to the Australian canola industry. Inoculum was present throughout all canola-growing regions of New South Wales and the stem disease was widespread throughout southern New South Wales. Percentage petal infestation increased over the 3 years surveyed with values ranging from 0 to 99.4%. The highest petal infestation values were observed in 2000 (maximum of 99.4%, mean of 82.2%), with lower mean values in 1998 (38.4%) and 1999 (49.6%). Stem infection ranged from 0 to 37.5% and most fields had less than 10% stem infection. Stem rot incidence before harvest did not relate to percentage petal infestation determined during flowering. This indicated that factors other than percentage petal infestation were important in influencing stem rot incidence. While there was no relationship between percentage petal infestation and stem rot incidence, stem infection never occurred without prior petal infestation.

scholarly journals Membrane Distillation for the Production of Pharmaceutical-Grade Water—Investigation into the Application of AGMD and VMD

2021 ◽  
Vol 18 (11) ◽  
pp. 6058
Author(s):  
Cornelius Nellessen ◽  
Thomas Klein ◽  
Hans-Jürgen Rapp ◽  
Frank Rögener
Keyword(s):  
Energy Demand ◽  
Tap Water ◽  
Membrane Distillation ◽  
Pharmaceutical Products ◽  
Optimum Process ◽  
The European Union ◽  
Pharmaceutical Ingredients ◽  
Multi Stage ◽  
One Step ◽  
Selection Of

The production of pharmaceutical ingredients, intermediates and final products strongly depends on the utilization of water. Water is also required for the purification and preparation of reagents. Each specific application determines the respective water quality. In the European Union, the European Pharmacopeia (Ph. Eur.) contains the official standards that assure quality control of pharmaceutical products during their life cycle. According to this, the production of water for pharmaceutical use is mainly based on multi-stage distillation and membrane processes, especially, reverse osmosis. Membrane distillation (MD) could be an alternative process to these classical methods. It offers advantages in terms of energy demand and a compact apparatus design. In the following study, the preparation of pharmaceutical-grade water from tap water in a one-step process using MD is presented. Special emphasis is placed on the performance of two different module designs and on the selection of optimum process parameters.

scholarly journals Selection of Trichoderma spp. strains for the control of Sclerotinia sclerotiorum in soybean

2017 ◽  
Vol 52 (12) ◽  
pp. 1140-1148 ◽  
Author(s):  
Patrícia Elias Haddad ◽  
Luis Garrigós Leite ◽  
Cleusa Maria Mantovanello Lucon ◽  
Ricardo Harakava
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Culture Medium ◽  
Harmful Effect ◽  
Soybean Seeds ◽  
Trichoderma Spp ◽  
Negative Effect ◽  
Soybean Plants ◽  
Selection Of

Abstract: The objective of this work was to evaluate, in vitro and in vivo, the potential of Trichoderma spp. strains to control Sclerotinia sclerotiorum in soybeans (Glycine max) and to perform the molecular identification of the best perfoming strains. The effect of 120 strains of Trichoderma spp. on the viability of S. sclerotiorum sclerotia was evaluated in vitro through immersion in suspension of conidia from the antagonists and plating in culture medium. The best performing strains were evaluated in vivo, in a greenhouse, for control of the pathogen inoculated on 'Pintado' soybean seeds and plants. Of the 120 strains tested in vitro, 22 strains of Trichoderma spp. caused 100% inhibition of sclerotia germination. In the greenhouse, five strains inhibited the negative effect of the pathogen on seed germination and two strains increased in up to 67% plant dry matter. The best performing strains were identified as T. koningiopsis (3 strains), T. asperelloides (3), T. atroviride (2), and T. virens (1). Trichoderma strains are able to protect soybean plants from the harmful effect of S. sclerotiorum and, at the same time, they can promote the growth of the aerial part in greenhouse conditions.

scholarly journals Environmental Factors for Germination of Sclerotinia sclerotiorum Sclerotia

2016 ◽  
Vol 7 (10) ◽  
Author(s):  
Michael E Foley ◽  
Münevver Dogramac ◽  
Mark West ◽  
William R Underwood
Keyword(s):  
Environmental Factors ◽  
Sclerotinia Sclerotiorum
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