scholarly journals Macroptilium yellow mosaic virus, a New Begomovirus Infecting Macroptilium lathyroides in Cuba

Plant Disease ◽  
2002 ◽  
Vol 86 (9) ◽  
pp. 1049-1049 ◽  
Author(s):  
P. L. Ramos ◽  
A. Fernández ◽  
G. Castrillo ◽  
L. Díaz ◽  
A. L. Echemendía ◽  
...  
Keyword(s):  
Amino Acid ◽  
Mosaic Virus ◽  
Restriction Fragment ◽  
Amino Acid Sequences ◽  
Yellow Mosaic Virus ◽  
Bipartite Begomovirus ◽  
Degenerate Primers ◽  
Cp Gene ◽  
Golden Yellow ◽  
Macroptilium Lathyroides

Macroptilium lathyroides (L) is a weed that is widely distributed in Cuba. Frequently, leaves show bright yellow mosaic symptoms, which suggest the incidence of a viral disease. Since begomovirus occurrence in Macroptilium lathyroides has been previously reported in other islands of the Caribbean (1,3), symptomatic plants from three distant places in Cuba (Havana, Villa Clara, and Camaguey), were collected and tested for the presence of begomoviruses. Plant DNA extracts were analyzed by Southern blot hybridization and polymerase chain reaction with two sets of degenerate primers (2). The presence of a bipartite begomovirus was evident through strong hybridization signals obtained with the DNA-A and DNA-B of Taino tomato mottle virus as probes at low stringency. Furthermore, 1.4-kb and 1.2-kb PCR amplified fragments were obtained with DNA-A degenerate primers, PAL1v1978-PAR1c715 and PAL1c1960-PAR1v722, respectively. Both PCR fragments from the samples from the three locations were cloned, and restriction fragment length polymorphism analysis of the 1.4-kb fragments were performed using PstI, EcoRI, HincII, XbaI and BglII. Restriction fragment patterns were the same for the three clones. The DNA-A sequence (GenBank Accession No. AJ344452) of the isolate from Villa Clara was compared with sequences available for other geminiviruses using CLUSTAL program. For the coat protein (CP) gene, the comparisons had the highest percentage of identity with various strains of Bean golden yellow mosaic virus (BGYMV, GenBank Accession Nos. AF173555, M91604, and L01635) (85 to 87% and 93 to 94%, nucleotide and amino acid sequences, respectively). For Rep gene (1,044 nt), the best percentages of identities were with BGYMV (81 to 82% and 80 to 82% nucleotide and amino acid sequences, respectively), Tomato leaf crumple virus (GenBank Accession No. AF101476) (78 and 81%, nucleotide and amino acid sequences, respectively), and Sida golden mosaic virus from Florida (GenBank Accession No. AF049336) (78 and 79%, nucleotide and amino acid sequences, respectively). Finally, the comparative analysis of the intergenic region (i.e. the common region plus the CP gene promoter) had the highest identity with BGYMV (56 to 55%) and Tomato severe rugose virus (GenBank Accession No. AY029750) (49%). Interestingly, this virus has in this region the three G-box elements that are characteristic of BGYMV but it differs in the Rep protein-binding iterative motif that is GGTGA instead of GGAGA, for BGYMV. These data indicate that this virus is a new begomovirus and the name of Macroptilium yellow mosaic virus (MaYMV) is proposed. References: (1) A. M. Idris et al. Plant Dis. 83:1071, 1999. (2) M. R. Rojas et al. Plant Dis. 77:340, 1993. (3) M. E. Roye et al. Plant Dis. 81:1251, 1997.

scholarly journals Genetic Diversity Among Geminiviruses Associated with the Weed Species Sida spp., Macroptilium lathyroides, and Wissadula amplissima from Jamaica

Plant Disease ◽  
1997 ◽  
Vol 81 (11) ◽  
pp. 1251-1258 ◽  
Author(s):  
Marcia E. Roye ◽  
Wayne A. McLaughlin ◽  
Medhat K. Nakhla ◽  
Douglas P. Maxwell
Keyword(s):  
Genetic Diversity ◽  
Amino Acid ◽  
Mosaic Virus ◽  
Amino Acid Sequences ◽  
Yellow Mosaic Virus ◽  
Weed Species ◽  
Degenerate Primers ◽  
Sequence Comparisons ◽  
The Common ◽  
Macroptilium Lathyroides

Genetic diversity among geminiviruses associated with three common weeds in Jamaica was studied using digoxigenin-labeled geminiviral DNA probes, polymerase chain reaction with degenerate primers for DNA-A and DNA-B, nucleic acid sequencing, and derived amino acid sequences. Geminiviruses with bipartite genomes were found in Sida spp., Macroptilium lathyroides, and Wissadula amplissima. The geminiviruses detected in Sida spp. and M. lathyroides were nearly identical and were both designated Sida golden mosaic geminivirus (SidGMV-JA), whereas the geminivirus in W. amplissima was sufficiently different to be designated Wissadula golden mosaic geminivirus (WGMV). Nucleotide sequence comparisons of the common regions and the N-terminal regions of the AC1 (rep) and AV1 ORFs, together with the derived amino acid sequence comparisons of the N-terminal parts of BC1 and BV1 ORFs were used to determine their similarities to other geminiviruses. SidGMV-JA was most similar to potato yellow mosaic geminivirus (PYMV). We propose that these two geminiviruses (SidGMV-JA and PYMV) define a new geminivirus cluster, the potato yellow mosaic virus (PYMV) cluster. WGMV was most similar to members of the Abutilon mosaic virus cluster but is not likely to be included in the Abutilon phylogenetic group because of the divergent sequence of the common region. These results indicate that geminiviruses infecting some weeds in Jamaica are distinct from crop-infecting geminiviruses in Jamaica and define a new geminivirus cluster.

scholarly journals DNA Analysis Confirms Macroptilium lathyroides as Alternative Host of Bean golden yellow mosaic virus

Plant Disease ◽  
2003 ◽  
Vol 87 (9) ◽  
pp. 1022-1025 ◽  
Author(s):  
V. Bracero ◽  
L. I. Rivera ◽  
J. S. Beaver
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Nucleotide Sequence Analysis ◽  
Alternative Host ◽  
Degenerate Primers ◽  
Viral Dna ◽  
Golden Yellow ◽  
Macroptilium Lathyroides

The leguminous weed Macroptilium lathyroides is considered a potential host of the Bean golden yellow mosaic virus (BGYMV; BGMV = Mesoamerican isolates). To determine if M. lathyroides could be a host for BGYMV, an infectivity cycle was established between this weed and Phaseolus vulgaris. Virus transmission was carried out using the whitefly, Bemisia argentifolli, as a vector. Inoculated plants of both species were examined for symptoms such as mosaic, stunting, and leaf distortion. P. vulgaris and M. lathyroides showed golden yellow mosaic symptoms during all infectivity cycle stages. Symptomatic plants of both species were tested for BGYMV using polymerase chain reaction (PCR) and nucleotide sequence analysis. Two degenerate primers sets were used for PCR to detect viral DNA: PAL1v1978/PAR1c715 and PCRc2/PBL12039. PCR analysis using primers PCRc2/PBL12039 amplified viral DNA for component B from both plant species. Nucleotide sequence analysis revealed a 93% identity between the virus isolated from M. lathyroides and the Puerto Rican isolate of BGYMV. These results confirmed that M. lathyroides could serve as an alternative host of BGYMV and that an infectivity cycle of BGYMV could possibly occur between P. vulgaris and M. lathyroides in Puerto Rico.

scholarly journals First Report of Papaya ringspot virus–Type W and Zucchini yellow mosaic virus Infecting Trichosanthes cucumerina in Brazil

Plant Disease ◽  
2010 ◽  
Vol 94 (6) ◽  
pp. 789-789 ◽  
Author(s):  
A. S. Jadão ◽  
J. E. Buriola ◽  
J. A. M. Rezende
Keyword(s):  
Amino Acid ◽  
Mosaic Virus ◽  
Virus Type ◽  
Zucchini Yellow Mosaic Virus ◽  
Amino Acid Sequences ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Mechanical Inoculation ◽  
Cp Gene

Trichosanthes cucumerina L., known as snake gourd, is a cucurbitaceous plant that is probably native to and originally domesticated in India. It is cultivated in humid subtropical and tropical countries of Australia, Latin America, and Africa (2). Plants of this species exhibiting symptoms of mosaic and leaf malformation were found during November 2008 near an experimental field of the Departamento de Fitopatologia e Nematologia, Universidade de São Paulo, Piracicaba, State of São Paulo, Brazil. Electron microscopy examination of negatively stained extract of infected tissue showed the presence of filamentous potyvirus-like particles. Sap from these infected plants reacted in plate-trapped antigen (PTA)-ELISA with the antiserum against Papaya ringspot virus–type W (PRSV-W) or Zucchini yellow mosaic virus (ZYMV), but not with the antiserum against Cucumber mosaic virus (CMV) or Zucchini lethal chlorosis virus (ZLCV). PRSV-W and ZYMV were simultaneously transmitted by mechanical inoculation to four plants of Cucurbita pepo cv. Caserta and one plant of T. cucumerina, causing mosaic. In addition, PRSV-W and ZYMV isolates from our virus collection separately infected one plant of T. cucumerina after mechanical inoculation. Infections were confirmed by PTA-ELISA. Total RNA extracted from infected and healthy T. cucumerina was analyzed by reverse transcription (RT)-PCR using a primer pair specific to the coat protein (CP) gene of PRSV-W (4) or ZYMV (3). Fragments of 864 bp and 1,045 bp were amplified with each pair of primers, respectively. Nucleotide sequences directly obtained from purified PCR products were used for further identification of these potyviruses. The nucleotide and deduced amino acid sequences of part of the CP gene (792 nt) of PRSV-W (GenBank Accession No. GU586789) shared 99 and 98% identity, respectively, with that of the Brazilian isolate PRSV-W-C (GenBank Accession No. 4152). The nucleotide and deduced amino acid sequences of the entire CP gene (837 nt) of ZYMV (GenBank Accession No. 6790) shared 91 to 98% and 94 to 100% identity, respectively, with innumerous isolates of ZYMV deposited in the GenBank (e.g., Accession Nos. AB004640, D13914, AB004641, and AJ420019). Natural infection of T. cucumerina by PRSV-W was reported in Nepal (1). To our knowledge, this is the first report of T. cucumerina infected by PRSV-W and ZYMV in Brazil. References: (1) G. Dahal et al. Ann. Appl. Biol. 130:491, 1997. (2) R. W. Robinson and D. S. Decker-Walters. Cucurbits. CAB International, Wallingford, UK. 1997. (3) K. G. Thomson et al. J. Virol. Methods 55:83, 1995. (4) M. G. S. D. Vechia. Fitopatol. Bras. 28:678, 2003.

scholarly journals Detecting bean golden yellow mosaic virus in bean breeding lines and in the common legume weed Macroptilium lathyroides in Puerto Rico.

1969 ◽  
Vol 85 (3-4) ◽  
pp. 165-176
Author(s):  
Lydia I. Rivera-Vargas ◽  
Vilmaris Bracero-Acosta ◽  
James S. Beaver ◽  
Dan E. Purcifull ◽  
Jane E. Polston ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Phaseolus Vulgaris L ◽  
Breeding Lines ◽  
Golden Yellow ◽  
Enzymelinked Immunosorbent Assay ◽  
The Common ◽  
Polymerase Chain ◽  
Macroptilium Lathyroides

Bean golden yellow mosaic virus (BGYMV) is a geminivirus transmitted by whiteflies (Genus: Bemisia). This virus causes significant fosses in common bean (Phaseolus vulgaris L.). Serological techniques such as enzymelinked immunosorbent assay (ELISA) have been widely used for detection of viruses. We evaluated existing monoclonal antibodies (3F7,2G5 and 5C5) for the detection of BGYMV isolates in bean fines in Puerto Rico. Monoclonal antibody 3F7 was the most effective in detecting the virus in tissues of line DOR 364 and susceptible cuftivars Top Crop and Quest. However, it was not effective in the detection of BGYMV in lines of DOR 303, which showed typical symptoms. Sampfes from Macroptilium lathyroides, a weed that might be a possible reservoir of the virus, were also tested for viraf infection. ELISA tests were inconclusive for detection of geminiviruses in M. lathyroides. Polymerase Chain Reaction (PCR) was also used to complement BGYMV diagnosis in M. lathyroides and in bean lines that showed symptoms but were negative for the ELfSA test. Two sets of primers, specific for Begomovirus such as BGYMV, were used in PCR experiments. Using PCR, we were able to detect the virus in the line DOR 303 and in M. lathyroides tissues.

scholarly journals Two Newly Described Begomoviruses of Macroptilium lathyroides and Common Bean

2003 ◽  
Vol 93 (7) ◽  
pp. 774-783 ◽  
Author(s):  
A. M. Idris ◽  
E. Hiebert ◽  
J. Bird ◽  
J. K. Brown
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Dna Sequences ◽  
Western Hemisphere ◽  
Yellow Mosaic Virus ◽  
Nucleotide Identity ◽  
Caribbean Region ◽  
Golden Yellow ◽  
The Caribbean ◽  
Macroptilium Lathyroides

Macroptilium lathyroides, a perennial weed in the Caribbean region and Central America, is a host of Macroptilium yellow mosaic Florida virus (MaYMFV) and Macroptilium mosaic Puerto Rico virus (MaMPRV). The genomes of MaYMFV and MaMPRV were cloned from M. lathyroides and/or field-infected bean and the DNA sequences were determined. Cloned A and B components for both viruses were infectious when inoculated to M. lathyroides and common bean. Comparison of the DNA sequences for cloned A and B components with well-studied begomovirus indicated that MaMPRV (bean and M. lathyroides) and MaYMFV (M. lathyroides) are unique, previously undescribed begomo-viruses from the Western Hemisphere. Phylogenetic analysis of viral A components indicated that the closest relative of MaYMFV are members of the Bean golden yellow mosaic virus (BGYMV) group, at 76 to 78% nucleotide identity, whereas the closest relative for the A component of MaMPRV was Rhynchosia golden mosaic virus at 78% nucleotide identity. In contrast, BGYMV is the closest relative for the B component of both MaYMFV and MaMPRV, with which they share ≈68.0 and ≈72% identity, respectively. The incongruent taxonomic placement for the bipartite components for MaMPRV indicates that they did not evolve entirely along a common path. MaYMFV and MaMPRV caused distinctive symptoms in bean and M. lathyroides and were transmissible by the whitefly vector and by grafting; however, only MaYMFV was mechanically transmissible. The experimental host range for the two viruses was similar and included species within the families Fabaceae and Malvaceae, but only MaYMFV infected Malva parviflora and soybean. These results collectively indicate that MaMPRV and MaYMFV are new, previously undescribed species of the BGYMV group, a clade previously known to contain only strains and isolates of BGYMV from the Caribbean region that infect Phaseolus spp. Both MaYMFV and MaMPRV may pose an economic threat to bean production in the region.

scholarly journals IDENTIFIKASI MOLEKULER BEAN COMMON MOSAIC VIRUS YANG BERASOSIASI DENGAN PENYAKIT MOSAIK KUNING KACANG PANJANG

2016 ◽  
Vol 15 (2) ◽  
pp. 132
Author(s):  
Melinda . ◽  
Tri Asmira Damayanti ◽  
Sri Hendrastuti Hidayat
Keyword(s):  
Amino Acid ◽  
Mosaic Virus ◽  
Bean Common Mosaic Virus ◽  
Mosaic Disease ◽  
Amino Acid Sequences ◽  
Yellow Mosaic Disease ◽  
West Java ◽  
Central Java ◽  
Cp Gene ◽  
Dna Fragment

Molecular identification of bean common mosaic virus associated with yellow mosaic disease on yard long bean. Bean common mosaic virus (BCMV) has been reported as one of the causal agents of yellow mosaic disease on yard long bean in West Java and Central Java. Infected plants showed mosaic, yellowing, and mixture of yellow mosaic. The research was conducted to identify the diversity of BCMV associated with yellow mosaic disease based on coat protein (CP) gene sequences. Symptomatic leaf samples were collected from yard long bean growing areas in several districts in West Java (Bogor, Cirebon, Subang, and Indramayu), and several districts in Central Java (Tegal, Klaten, Solo, Yogjakarta, Sleman, and Magelang). Molecular detection using RT-PCR method was carried out by using specific primer to BCMV which will amplify the CP gene. DNA fragment, + 860 bp in size, was successfully amplified from 8 out of 13 leaf samples, i.e samples from three villages in Bogor District (Cangkurawok, Bubulak, Bojong), and five samples from District of Cirebon, Subang, Solo, Sleman, and Tegal. Sequence analysis of those DNA fragment showed that 4 isolates (Bogor-Cangkurawok, Subang, Solo and Sleman) had the highest homology to BCMV-BlC from Taiwan, whereas 2 isolates (Cirebon and Tegal) had the highest homology to BCMVNL1 from England. Further, phyllogenetic analysis revealed that those of 4 isolates were closely related to BCMV-BlC from Taiwan based on nucleotide as well as amino acid sequences; while those other 2 isolates were closely related to BCMV-NL1 from England based on nucleotide sequences but closely related to BCMV-BlC Y from China based on amino acid sequences. Phyllogenetic analysis showed that those of 6 BCMV isolates separated in two different clusters; 4 isolates (Bogor- Cangkurawok, Subang, Solo, and Sleman) in cluster 1 together with BCMV-BlC from Taiwan, while other 2 isolates (Cirebon and Tegal) in cluster 2 together with BCMV-NL1.

scholarly journals Taino Tomato Mottle Virus, a New Bipartite Geminivirus from Cuba

Plant Disease ◽  
1997 ◽  
Vol 81 (9) ◽  
pp. 1095-1095 ◽  
Author(s):  
P. L. Ramos ◽  
O. Guerra ◽  
R. Peral ◽  
P. Oramas ◽  
R. G. Guevara ◽  
...  
Keyword(s):  
Amino Acid ◽  
Mosaic Virus ◽  
Nucleotide Sequences ◽  
Amino Acid Sequences ◽  
Mottle Virus ◽  
Yellow Mosaic Virus ◽  
Viral Dna ◽  
Common Region ◽  
Field Samples ◽  
Pepper Plants

Geminiviruses have become the most important virus group affecting tomatoes (Lycopersicon lycopersicum (L.) Karsten) in Cuba since they have been detected in all tomato-producing areas, causing serious losses. Recently, a whitefly-transmitted, monopartite geminivirus was detected in Cuba and identified as tomato yellow leaf curl virus-Israel (TYLCV-Is) (1). Samples collected from the main tomato-producing areas during the period 1995 to1996 were further analyzed by polymerase chain reaction (PCR) with degenerate primers (PAL1v1978 and PAR1c496) (2). Whereas in samples from most areas only TYLCV was detected, in some samples from the Havana area, two DNA fragments (approximately 1.4 and 1.1 kb) were amplified by PCR. The larger fragment was identified as part of the TYLCV-Is genome, confirming the previous report (1). The 1.1-kb fragment was cloned and its nucleotide sequence suggested that a new bipartite geminivirus was also present in those tomato samples. To clone the entire genome, tomato plants were inoculated by biolistics with DNA extract from field samples. After symptom expression, a viral DNA-enriched preparation from the inoculated tomatoes was independently digested with several restriction enzymes and the products were ligated into pZero plasmid (Invitrogen, San Diego, CA). Several clones in the 2.6-kb size range were characterized by restriction mapping and hybridization against component A and B heterologous probes. Two clones were selected as containing putative A and B components and their infectivity was tested by biolistic inoculation of tomato and pepper plants. The inoculated tomatoes developed a mild mottle in the younger leaves, whereas no symptoms were visible on the inoculated pepper plants. However, the presence of viral DNA was confirmed in both tomatoes and peppers by Southern blot hybridization analysis with A- and B-specific probes. Partial sequences of both components were obtained and their analysis showed that both components shared a 170-bases common region with a 95% identity. In addition, the nucleotide sequences of two open reading frames, one in each component (AC1 and BC1), were determined and compared with geminivirus sequences deposited in Gen-Bank. A dendogram generated with the CLUSTAL program and obtained with the AC1 and BC1 amino acid sequences, placed the new geminivirus in a cluster with tomato mottle virus (ToMoV; accession nos. L14460, L14461), Abutilon mosaic virus (AbMV; X15983, X15984), potato yellow mosaic virus (PYMV; D00940, D00941), and bean dwarf mosaic virus (BDMV; M88179, M88180). The percentages of identity obtained with the amino acid sequences were as follows. For AC1: ToMoV, 87%; PYMV, 79.5%; BDMV, 78.7%; and AbMV, 78%. For BC1 protein: BDMV, 92.8%; ToMoV, 89.1%; PYMV, 88.1%; and AbMV, 67.5%. In addition, the sequences were compared with partial nucleotide sequences (AC1, coat protein [CP], and common region) of a bipartite geminivirus affecting tomatoes in Jamaica (accession nos. U83855, U83854, and U83850). Interestingly, the common regions showed a higher percentage of identity (88%) than the CP and AC1 partial nucleotide sequences (86 and 74%, respectively). These data suggest that the virus reported here is a new geminivirus and the first bipartite geminivirus reported in Cuba. Thus, the name of Taino tomato mottle virus is proposed. (Taino refers to the name of the inhabitants of Cuba at the time of Columbus's arrival in the Caribbean). References: (1) P. L. Ramos et al. Plant Dis. 80:1208, 1996. (2) M. R. Rojas et al. Plant Dis. 77:340, 1993.

scholarly journals Identifying Resistance Gene Analogs Associated With Resistances to Different Pathogens in Common Bean

2003 ◽  
Vol 93 (1) ◽  
pp. 88-95 ◽  
Author(s):  
Camilo E. López ◽  
Iván F. Acosta ◽  
Carlos Jara ◽  
Fabio Pedraza ◽  
Eliana Gaitán-Solís ◽  
...  
Keyword(s):  
Common Bean ◽  
Resistance Gene ◽  
Plant Disease Resistance ◽  
Amino Acid Sequences ◽  
Yellow Mosaic Virus ◽  
Resistance Gene Analogs ◽  
R Genes ◽  
Degenerate Primers ◽  
Golden Yellow ◽  
Major Cluster

A polymerase chain reaction approach using degenerate primers that targeted the conserved domains of cloned plant disease resistance genes (R genes) was used to isolate a set of 15 resistance gene analogs (RGAs) from common bean (Phaseolus vulgaris). Eight different classes of RGAs were obtained from nucleotide binding site (NBS)-based primers and seven from not previously described Toll/Interleukin-1 receptor-like (TIR)-based primers. Putative amino acid sequences of RGAs were significantly similar to R genes and contained additional conserved motifs. The NBS-type RGAs were classified in two subgroups according to the expected final residue in the kinase-2 motif. Eleven RGAs were mapped at 19 loci on eight linkage groups of the common bean genetic map constructed at Centro Internacional de Agricultura Tropical. Genetic linkage was shown for eight RGAs with partial resistance to anthracnose, angular leaf spot (ALS) and Bean golden yellow mosaic virus (BGYMV). RGA1 and RGA2 were associated with resistance loci to anthracnose and BGYMV and were part of two clusters of R genes previously described. A new major cluster was detected by RGA7 and explained up to 63.9% of resistance to ALS and has a putative contribution to anthracnose resistance. These results show the usefulness of RGAs as candidate genes to detect and eventually isolate numerous R genes in common bean.

scholarly journals Weed-infecting viruses in a tropical agroecosystem present different threats to crops and evolutionary histories

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250066
Author(s):  
Minor R. Maliano ◽  
Mônica A. Macedo ◽  
Maria R. Rojas ◽  
Robert L. Gilbertson
Keyword(s):  
Phylogenetic Analyses ◽  
Bipartite Begomoviruses ◽  
Yellow Mosaic Virus ◽  
Bipartite Begomovirus ◽  
Caribbean Basin ◽  
Degenerate Primers ◽  
Infectious Clones ◽  
Bean Plants ◽  
Golden Yellow ◽  
The Caribbean

In the Caribbean Basin, malvaceous weeds commonly show striking golden/yellow mosaic symptoms. Leaf samples from Malachra sp. and Abutilon sp. plants with these symptoms were collected in Hispaniola from 2014 to 2020. PCR tests with degenerate primers revealed that all samples were infected with a bipartite begomovirus, and sequence analyses showed that Malachra sp. plants were infected with tobacco leaf curl Cuba virus (TbLCuCV), whereas the Abutilon sp. plants were infected with a new bipartite begomovirus, tentatively named Abutilon golden yellow mosaic virus (AbGYMV). Phylogenetic analyses showed that TbLCuCV and AbGYMV are distinct but closely related species, which are most closely related to bipartite begomoviruses infecting weeds in the Caribbean Basin. Infectious cloned DNA-A and DNA-B components were used to fulfilled Koch’s postulates for these diseases of Malachra sp. and Abutilon sp. In host range studies, TbLCuCV also induced severe symptoms in Nicotiana benthamiana, tobacco and common bean plants; whereas AbGYMV induced few or no symptoms in plants of these species. Pseudorecombinants generated with the infectious clones of these viruses were highly infectious and induced severe symptoms in N. benthamiana and Malachra sp., and both viruses coinfected Malachra sp., and possibly facilitating virus evolution via recombination and pseudorecombination. Together, our results suggest that TbLCuCV primarily infects Malachra sp. in the Caribbean Basin, and occasionally spills over to infect and cause disease in crops; whereas AbGYMV is well-adapted to an Abutilon sp. in the Dominican Republic and has not been reported infecting crops.

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