scholarly journals Liver microRNAs are differentially expressed in human simple steatosis and non alcoholic steatohepatitis with potential repercussions on lipid metabolism and inflammatory status

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Elena Maria Comelli ◽  
TaeHyung Kim ◽  
Bianca M Arendt ◽  
Natasha Singh ◽  
Zhaolei Zhang ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Differentially Expressed ◽  
Simple Steatosis ◽  
Alcoholic Steatohepatitis ◽  
Inflammatory Status

scholarly journals Characterization of Differentially Expressed Circulating miRNAs in Metabolically Healthy versus Unhealthy Obesity

Biomedicines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 321
Author(s):  
Susana Rovira-Llopis ◽  
Rubén Díaz-Rúa ◽  
Carmen Grau-del Valle ◽  
Francesca Iannantuoni ◽  
Zaida Abad-Jimenez ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Metabolism ◽  
Insulin Signalling ◽  
Cross Sectional Study ◽  
Differentially Expressed ◽  
Circulating Mirnas ◽  
Oxidative Stress Biomarkers ◽  
Cross Sectional ◽  
Metabolically Healthy Obese ◽  
Metabolically Healthy

Obese individuals without metabolic comorbidities are categorized as metabolically healthy obese (MHO). MicroRNAs (miRNAs) may be implicated in MHO. This cross-sectional study explores the link between circulating miRNAs and the main components of metabolic syndrome (MetS) in the context of obesity. We also examine oxidative stress biomarkers in MHO vs. metabolically unhealthy obesity (MUO). We analysed 3536 serum miRNAs in 20 middle-aged obese individuals: 10 MHO and 10 MUO. A total of 159 miRNAs were differentially expressed, of which, 72 miRNAs (45.2%) were higher and 87 miRNAs (54.7%) were lower in the MUO group. In addition, miRNAs related to insulin signalling and lipid metabolism pathways were upregulated in the MUO group. Among these miRNAs, hsa-miR-6796-5p and hsa-miR-4697-3p, which regulate oxidative stress, showed significant correlations with glucose, triglycerides, HbA1c and HDLc. Our results provide evidence of a pattern of differentially expressed miRNAs in obesity according to MetS, and identify those related to insulin resistance and lipid metabolism pathways.

scholarly journals High-throughput screening of circRNAs reveals novel mechanisms of tuberous sclerosis complex-related renal angiomyolipoma

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Yang Zhao ◽  
Hao Guo ◽  
Wenda Wang ◽  
Guoyang Zheng ◽  
Zhan Wang ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Tuberous Sclerosis ◽  
Tuberous Sclerosis Complex ◽  
Regulatory Network ◽  
High Throughput Screening ◽  
Kidney Tissue ◽  
Cell Types ◽  
The Body ◽  
Differentially Expressed ◽  
Renal Angiomyolipoma

Abstract Objective Tuberous sclerosis complex (TSC) is a rare autosomal dominant disease characterized by lesions throughout the body. Our previous study showed the abnormal up-regulation of miRNAs plays an important part in the pathogenesis of TSC-related renal angiomyolipoma (TSC-RAML). circRNAs were known as important regulators of miRNA, but little is known about the circRNAs in TSC-RAMLs. Methods Microarray chips and RNA sequencing were used to identify the circRNAs and mRNAs that were differently expressed between the TSC-RAML and normal kidney tissue. A competitive endogenous RNA (ceRNA) regulatory network was constructed to reveal the regulation of miRNAs and mRNAs by the circRNAs. The biological functions of circRNA and mRNA were analyzed by pathway analysis. Microenvironmental cell types were estimated with the MCP-counter package. Results We identified 491 differentially expressed circRNAs (DECs) and 212 differentially expressed genes (DEGs), and 6 DECs were further confirmed by q-PCR. A ceRNA regulatory network which included 6 DECs, 5 miRNAs, and 63 mRNAs was established. Lipid biosynthetic process was significantly up-regulated in TSC-RAML, and the humoral immune response and the leukocyte chemotaxis pathway were found to be down-regulated. Fibroblasts are enriched in TSC-RAML, and the up-regulation of circRNA_000799 and circRNA_025332 may be significantly correlated to the infiltration of the fibroblasts. Conclusion circRNAs may regulate the lipid metabolism of TSC-RAML by regulation of the miRNAs. Fibroblasts are enriched in TSC-RAMLs, and the population of fibroblast may be related to the alteration of circRNAs of TSC-RAML. Lipid metabolism in fibroblasts is a potential treatment target for TSC-RAML.

Baicalin attenuates non-alcoholic steatohepatitis by suppressing key regulators of lipid metabolism, inflammation and fibrosis in mice

Life Sciences ◽  
2018 ◽  
Vol 192 ◽  
pp. 46-54 ◽  
Author(s):  
Junli Zhang ◽  
Haiming Zhang ◽  
Xiaoling Deng ◽  
Ning Zhang ◽  
Beibei Liu ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Alcoholic Steatohepatitis

scholarly journals Integrated Analysis of Liver Transcriptome, miRNA, and Proteome of Chinese Indigenous Breed Ningxiang Pig in Three Developmental Stages Uncovers Significant miRNA–mRNA–Protein Networks in Lipid Metabolism

2021 ◽  
Vol 12 ◽  
Author(s):  
Biao Li ◽  
Jinzeng Yang ◽  
Yan Gong ◽  
Yu Xiao ◽  
Qinghua Zeng ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Expression Patterns ◽  
Immune Defense ◽  
Differentially Expressed ◽  
Integrated Analysis ◽  
Liver Development ◽  
Liver Growth ◽  
Functional Transformation ◽  
Functional Changes ◽  
Glycolipid Metabolism

Liver is an important metabolic organ of mammals. During each transitional period of life, liver metabolism is programmed by a complex molecular regulatory system for multiple physiological functions, many pathways of which are regulated by hormones and cytokines, nuclear receptors, and transcription factors. To gain a comprehensive and unbiased molecular understanding of liver growth and development in Ningxiang pigs, we analyzed the mRNA, microRNA (miRNA), and proteomes of the livers of Ningxiang pigs during lactation, nursery, and fattening periods. A total of 22,411 genes (19,653 known mRNAs and 2758 novel mRNAs), 1122 miRNAs (384 known miRNAs and 738 novel miRNAs), and 1123 unique proteins with medium and high abundance were identified by high-throughput sequencing and mass spectrometry. We show that the differences in transcriptional, post-transcriptional, or protein levels were readily identified by comparing different time periods, providing evidence that functional changes that may occur during liver development are widespread. In addition, we found many overlapping differentially expressed genes (DEGs)/differentially expressed miRNAs (DEMs)/differentially expressed proteins (DEPs) related to glycolipid metabolism in any group comparison. These overlapping DEGs/DEMs/DGPs may play an important role in functional transformation during liver development. Short Time-series Expression Miner (STEM) analysis revealed multiple expression patterns of mRNA, miRNA, and protein in the liver. Furthermore, several diverse key Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including immune defense, glycolipid metabolism, protein transport and uptake, and cell proliferation and development, were identified by combined analysis of DEGs and DGPs. A number of predicted miRNA–mRNA–protein pairs were found and validated by qRT-PCR and parallel reaction monitoring (PRM) assays. The results provide new and important information about the genetic breeding of Ningxiang pigs, which represents a foundation for further understanding the molecular regulatory mechanisms of dynamic development of liver tissue, functional transformation, and lipid metabolism.

Combinational applicaton of silybin and tangeretin attenuates the progression of non-alcoholic steatohepatitis (NASH) in mice via modulating lipid metabolism

2020 ◽  
Vol 151 ◽  
pp. 104519 ◽  
Author(s):  
Rinkiko Suguro ◽  
Xiao-cong Pang ◽  
Zhong-wen Yuan ◽  
Si-yao Chen ◽  
Yi-Zhun Zhu ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Alcoholic Steatohepatitis

Integrative transcriptomics and metabolomics explore the mechanism of kaempferol on improving nonalcoholic steatohepatitis

2020 ◽  
Vol 11 (11) ◽  
pp. 10058-10069
Author(s):  
Yifei Lu ◽  
Mingmei Shao ◽  
Hongjiao Xiang ◽  
Peiyong Zheng ◽  
Tao Wu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Metabolism ◽  
Energy Metabolism ◽  
Nonalcoholic Steatohepatitis ◽  
Alcoholic Steatohepatitis ◽  
Integration Analysis

By integration analysis of transcriptomics and metabolomics, Kaempferol was found to improve non-alcoholic steatohepatitis in mice probably through regulating energy metabolism, lipid metabolism, oxidative stress and inflammation-related pathways.

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