scholarly journals Point‐of‐care cTnI Tests Accurately Predict Heat Stroke Severity; Proof of Concept for a Heat Stroke Field Test

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Gerald Audet ◽  
Carrie Quinn ◽  
Lisa Leon
Keyword(s):  
Field Test ◽  
Point Of Care ◽  
Heat Stroke ◽  
Stroke Severity ◽  
Proof Of Concept

Point-of-care cardiac troponin test accurately predicts heat stroke severity in rats

2015 ◽  
Vol 309 (10) ◽  
pp. R1264-R1272 ◽  
Author(s):  
Gerald N. Audet ◽  
Carrie M. Quinn ◽  
Lisa R. Leon
Keyword(s):  
Cardiac Troponin ◽  
Point Of Care ◽  
Heat Stroke ◽  
Heat Exposure ◽  
Field Tests ◽  
Organ Damage ◽  
Band Intensity ◽  
Health Concern ◽  
Clinical Test ◽  
Stroke Severity

Heat stroke (HS) remains a significant public health concern. Despite the substantial threat posed by HS, there is still no field or clinical test of HS severity. We suggested previously that circulating cardiac troponin (cTnI) could serve as a robust biomarker of HS severity after heating. In the present study, we hypothesized that (cTnI) point-of-care test (ctPOC) could be used to predict severity and organ damage at the onset of HS. Conscious male Fischer 344 rats ( n = 16) continuously monitored for heart rate (HR), blood pressure (BP), and core temperature (Tc) (radiotelemetry) were heated to maximum Tc (Tc,Max) of 41.9 ± 0.1°C and recovered undisturbed for 24 h at an ambient temperature of 20°C. Blood samples were taken at Tc,Max and 24 h after heat via submandibular bleed and analyzed on ctPOC test. POC cTnI band intensity was ranked using a simple four-point scale via two blinded observers and compared with cTnI levels measured by a clinical blood analyzer. Blood was also analyzed for biomarkers of systemic organ damage. HS severity, as previously defined using HR, BP, and recovery Tc profile during heat exposure, correlated strongly with cTnI ( R2 = 0.69) at Tc,Max. POC cTnI band intensity ranking accurately predicted cTnI levels ( R2 = 0.64) and HS severity ( R2 = 0.83). Five markers of systemic organ damage also correlated with ctPOC score (albumin, alanine aminotransferase, blood urea nitrogen, cholesterol, and total bilirubin; R2 > 0.4). This suggests that cTnI POC tests can accurately determine HS severity and could serve as simple, portable, cost-effective HS field tests.

Cardiovascular and thermoregulatory biomarkers of heat stroke severity in a conscious rat model

2014 ◽  
Vol 117 (9) ◽  
pp. 971-978 ◽  
Author(s):  
Carrie M. Quinn ◽  
Rocio M. Duran ◽  
Gerald N. Audet ◽  
Nisha Charkoudian ◽  
Lisa R. Leon
Keyword(s):  
Troponin I ◽  
Point Of Care ◽  
Multiorgan Failure ◽  
Heat Stroke ◽  
Heat Exposure ◽  
Fold Increase ◽  
Organ Damage ◽  
Stroke Severity ◽  
Conscious Rat ◽  
Novel Biomarkers

Multiorgan failure is a catastrophic consequence of heat stroke (HS) and considered the underlying etiology of mortality. Identifying novel biomarkers capable of predicting the extent of HS-induced organ damage will enhance point-of-care triage and treatment. Conscious male F344 rats ( n = 32) were radiotelemetered for continuous core temperature (Tc), heart rate, and arterial pressure measurement. Twenty-two animals were exposed to ambient temperature of 37°C to a maximum Tc of 41.9 ± 0.1°C. Rats were euthanized at 24 h of recovery for analysis of plasma biomarkers [cardiac troponin I (cTnI), blood urea nitrogen (BUN), alanine aminotransferase (ALT), albumin, glucose] and histology. Tc profiles observed during recovery stratified HS severity into Mild, Moderate, and Severe. Eleven (50%) animals exhibited an acute compensatory hemodynamic response to heat exposure and a monophasic Tc profile consisting of sustained hyperthermia (∼1°C). Five (23%) rats displayed hemodynamic challenge and a biphasic Tc profile with rapid return to baseline followed by rebound hyperthermia. All biomarkers were significantly altered from control values ( P < 0.05). Four (18%) animals exhibited significant hemodynamic compromise during heat and a triphasic profile characterized by rapid cooling to baseline Tc, rebound hyperthermia, and subsequent hypothermia (∼35°C) through 24 h. cTnI showed a 40-fold increase over CON ( P < 0.001) and correlated with BUN ( r = 0.912) consistent with cardiorenal failure. Hypoglycemia correlated with ALT ( r = 0.824) suggestive of liver dysfunction. Histology demonstrated myocardial infarction, renal tubular necrosis, and acute liver necrosis. Two (9%) animals succumbed during HS recovery. This study identified novel biomarkers that predict HS severity and organ damage during acute recovery that could provide clinical significance for identifying key biomarkers of HS pathogenesis.

Janus computerised prescribing system provides pharmacological knowledge at point of care – design, development and proof of concept

2006 ◽  
Vol 62 (4) ◽  
pp. 251-258 ◽  
Author(s):  
Marie Eliasson ◽  
Pia Bastholm ◽  
Peter Forsberg ◽  
Kjell Henriksson ◽  
Lennart Jacobson ◽  
...  
Keyword(s):  
Point Of Care ◽  
Proof Of Concept ◽  
Design Development ◽  
Computerised Prescribing

scholarly journals Novel Point-of-Care Diagnostic Method for Neonatal Encephalopathy Using Purine Nucleosides

2021 ◽  
Vol 14 ◽  
Author(s):  
Edward Beamer ◽  
Mary Isabel O’Dea ◽  
Aisling A. Garvey ◽  
Jonathon Smith ◽  
Aida Menéndez-Méndez ◽  
...  
Keyword(s):  
Point Of Care ◽  
High Sensitivity ◽  
Rapid Diagnosis ◽  
Proof Of Concept ◽  
Neonatal Encephalopathy ◽  
Roc Curve Analysis ◽  
Neurodevelopmental Outcomes ◽  
Molecular Tests ◽  
Bedside Test ◽  
Diagnostic Technology

Background: Evidence suggests that earlier diagnosis and initiation of treatment immediately after birth is critical for improved neurodevelopmental outcomes following neonatal encephalopathy (NE). Current diagnostic tests are, however, mainly restricted to clinical diagnosis with no molecular tests available. Purines including adenosine are released during brain injury such as hypoxia and are also present in biofluids. Whether blood purine changes can be used to diagnose NE has not been investigated to date.Methods: Blood purines were measured in a mouse model of neonatal hypoxia and infants with NE using a novel point-of-care diagnostic technology (SMARTChip) based on the summated electrochemical detection of adenosine and adenosine metabolites in the blood.Results: Blood purine concentrations were ∼2–3-fold elevated following hypoxia in mice [2.77 ± 0.48 μM (Control) vs. 7.57 ± 1.41 μM (post-hypoxia), p = 0.029]. Data in infants with NE had a 2–3-fold elevation when compared to healthy controls [1.63 ± 0.47 μM (Control, N = 5) vs. 4.87 ± 0.92 μM (NE, N = 21), p = 0.0155]. ROC curve analysis demonstrates a high sensitivity (81%) and specificity (80%) for our approach to identify infants with NE. Moreover, blood purine concentrations were higher in infants with NE and seizures [8.13 ± 3.23 μM (with seizures, N = 5) vs. 3.86 ± 0.56 μM (without seizures, N = 16), p = 0.044].Conclusion: Our data provides the proof-of-concept that measurement of blood purine concentrations via SMARTChip technology may offer a low-volume bedside test to support a rapid diagnosis of NE.

scholarly journals A proof of concept study for the differentiation of SARS-CoV-2, hCoV-NL63, and IAV-H1N1 in vitro cultures using ion mobility spectrometry

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Feuerherd ◽  
A.-K. Sippel ◽  
J. Erber ◽  
J. I. Baumbach ◽  
R. M. Schmid ◽  
...  
Keyword(s):  
Organic Compounds ◽  
Ion Mobility ◽  
Point Of Care ◽  
Respiratory Viruses ◽  
Gas Analysis ◽  
In Vitro Cultures ◽  
Proof Of Concept ◽  
Volatile Organic ◽  
Set Up

AbstractRapid, high-throughput diagnostic tests are essential to decelerate the spread of the novel coronavirus disease 2019 (COVID-19) pandemic. While RT-PCR tests performed in centralized laboratories remain the gold standard, rapid point-of-care antigen tests might provide faster results. However, they are associated with markedly reduced sensitivity. Bedside breath gas analysis of volatile organic compounds detected by ion mobility spectrometry (IMS) may enable a quick and sensitive point-of-care testing alternative. In this proof-of-concept study, we investigated whether gas analysis by IMS can discriminate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from other respiratory viruses in an experimental set-up. Repeated gas analyses of air samples collected from the headspace of virus-infected in vitro cultures were performed for 5 days. A three-step decision tree using the intensities of four spectrometry peaks correlating to unidentified volatile organic compounds allowed the correct classification of SARS-CoV-2, human coronavirus-NL63, and influenza A virus H1N1 without misassignment when the calculation was performed with data 3 days post infection. The forward selection assignment model allowed the identification of SARS-CoV-2 with high sensitivity and specificity, with only one of 231 measurements (0.43%) being misclassified. Thus, volatile organic compound analysis by IMS allows highly accurate differentiation of SARS-CoV-2 from other respiratory viruses in an experimental set-up, supporting further research and evaluation in clinical studies.

Point-of-care ultrasound in a multiplace hyperbaric chamber

2021 ◽  
pp. 221-226
Author(s):  
Kirsten Hornbeak ◽  
◽  
Jay Duchnick ◽  
Anthony Medak ◽  
Peter Lindholm ◽  
...  
Keyword(s):  
Imaging Modality ◽  
Point Of Care ◽  
Electronic Devices ◽  
Point Of Care Ultrasound ◽  
Clinical Settings ◽  
Proof Of Concept ◽  
Safety Standards ◽  
Patient Assessments ◽  
Hyperbaric Environment ◽  
Hbo2 Therapy

Historically, electronic devices have been generally prohibited during hyperbaric oxygen (HBO2) therapy due to risk of fire in a pressurized, oxygen-rich environment. Point-of-care ultrasound (POCUS), however, has emerged as a useful imaging modality in diverse clinical settings. Hyperbaric chambers treating critically ill patients would benefit from the application of POCUS at pressure to make real-time patient assessments. Thus far, POCUS during HBO2 therapy has been limited due to required equipment modifications to meet safety standards. Here we demonstrate proof of concept, safety, and successful performance of an off-the-shelf handheld POCUS system (SonoSite iViz) in a clinical hyperbaric environment without need for modification.

scholarly journals Prior viral illness increases heat stroke severity in mice

2020 ◽  
Vol 106 (1) ◽  
pp. 244-257 ◽  
Author(s):  
Shauna M. Dineen ◽  
Jermaine A. Ward ◽  
Lisa R. Leon
Keyword(s):  
Heat Stroke ◽  
Stroke Severity ◽  
Viral Illness

scholarly journals A Proof of Concept of a Mobile Health Application to Support Professionals in a Portuguese Nursing Home

Sensors ◽  
2019 ◽  
Vol 19 (18) ◽  
pp. 3951
Author(s):  
Márcia Esteves ◽  
Marisa Esteves ◽  
António Abelha ◽  
José Machado
Keyword(s):  
Nursing Home ◽  
Nursing Homes ◽  
Mobile Health ◽  
Health Professionals ◽  
Point Of Care ◽  
Healthcare Delivery ◽  
Proof Of Concept ◽  
Mobile Health Application ◽  
And Performance

Over the past few years, the rapidly aging population has been posing several challenges to healthcare systems worldwide. Consequently, in Portugal, nursing homes have been getting a higher demand, and health professionals working in these facilities are overloaded with work. Moreover, the lack of health information and communication technology (HICT) and the use of unsophisticated methods, such as paper, in nursing homes to clinically manage residents lead to more errors and are time-consuming. Thus, this article proposes a proof of concept of a mobile health (mHealth) application developed for the health professionals working in a Portuguese nursing home to support them at the point-of-care, namely to manage and have access to information and to help them schedule, perform, and digitally record their tasks. Additionally, clinical and performance business intelligence (BI) indicators to assist the decision-making process are also defined. Thereby, this solution aims to introduce technological improvements into the facility to improve healthcare delivery and, by taking advantage of the benefits provided by these improvements, lessen some of the workload experienced by health professionals, reduce time-waste and errors, and, ultimately, enhance elders’ quality of life and improve the quality of the services provided.

scholarly journals Switching from Multiplex to Multimodal Colorimetric Lateral Flow Immunosensor

Sensors ◽  
2020 ◽  
Vol 20 (22) ◽  
pp. 6609
Author(s):  
Simone Cavalera ◽  
Fabio Di Nardo ◽  
Luca Forte ◽  
Francesca Marinoni ◽  
Matteo Chiarello ◽  
...  
Keyword(s):  
Point Of Care ◽  
Lateral Flow ◽  
Proof Of Concept ◽  
Point Of Care Testing ◽  
Human Immunoglobulins ◽  
Immunodeficiency Virus ◽  
Specific Antigens ◽  
Increasing Demand ◽  
Gold Nanomaterials ◽  
Two Parameter

Multiplex lateral flow immunoassay (LFIA) is largely used for point-of-care testing to detect different pathogens or biomarkers in a single device. The increasing demand for multitargeting diagnostics requires multi-informative single tests. In this study, we demonstrated three strategies to upgrade standard multiplex LFIA to multimodal capacity. As a proof-of-concept, we applied the strategies to the differential diagnosis of Human Immunodeficiency Virus (HIV) infection, a widespread pathogen, for which conventional multiplex LFIA testing is well-established. In the new two-parameter LFIA (x2LFIA), we exploited color encoding, in which the binding of multiple targets occurs in one reactive band and the color of the probe reveals which one is present in the sample. By combining the sequential alignment of several reactive zones along the membrane of the LFIA strip and gold nanoparticles and gold nanostars for the differential visualization, in this demonstration, the x2LFIA can furnish information on HIV serotype and stage of infection in a single device. Three immunosensors were designed. The use of bioreagents as the capturing ligand anchored onto the membrane or as the detection ligand labelled with gold nanomaterials affected the performance of the x2LFIA. Higher detectability was achieved by the format involving the HIV-specific antigens as capturing agent and labelled secondary bioligands (anti-human immunoglobulins M and protein G) as the probes.

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