Detection and Quantitative Analysis of Early Stage Orthotopic Murine Bladder Tumor Using In Vivo Magnetic Resonance Imaging

Abstract Background Hepatocellular carcinoma (HCC) is a common malignant tumor with poor prognosis. Magnetic resonance imaging (MRI) is one of the most effective imaging methods for the early diagnosis of HCC. However, the current MR contrast agents are still facing challenges in the early diagnosis of HCC due to their relatively low sensitivity and biosafety. Thus, the development of effective MR agents is highly needed for the early diagnosis of HCC. Results Herein, we fabricated an HCC-targeted nanocomplexes containing SPIO-loaded mesoporous polydopamine (MPDA@SPIO), sialic acid (SA)-modified polyethyleneimine (SA-PEI), and alpha-fetoprotein regulated ferritin gene (AFP-Fth) which was developed for the early diagnosis of HCC. It was found that the prepared nanocomplexes (MPDA@SPIO/SA-PEI/AFP-Fth) has an excellent biocompatibility towards the liver cells. In vivo and in vivo studies revealed that the transfection of AFP-Fth gene in hepatic cells significantly upregulated the expression level of ferritin, thereby resulting in an enhanced contrast on T2-weighted images via the formed endogenous MR contrast. Conclusions The results suggested that MPDA@SPIO/SA-PEI/AFP-Fth had a superior ability to enhance the MR contrast of T2-weighted images of tumor region than the other preparations, which was due to its HCC-targeted ability and the combined T2 contrast effect of endogenous ferritin and exogenous SPIO. Our study proved that MPDA@SPIO/SA-PEI/AFP-Fth nanocomplexes could be used as an effective MR contrast agent to detect HCC in the early stage.

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The in vivo diagnosis of early-stage aortic valve sclerosis using magnetic resonance imaging in a rabbit model

Journal of Magnetic Resonance Imaging ◽

10.1002/jmri.21729 ◽

2009 ◽

Vol 29 (4) ◽

pp. 825-831 ◽

Cited By ~ 4

Author(s):

Amanda M. Hamilton ◽

Kem A. Rogers ◽

Maria Drangova ◽

Zamir Khan ◽

John A. Ronald ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Aortic Valve ◽

Early Stage ◽

Rabbit Model ◽

Resonance Imaging ◽

Aortic Valve Sclerosis ◽

In Vivo Diagnosis

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Quantitative Analysis of Lipid-Rich Necrotic Core in Carotid Atherosclerotic Plaques by In Vivo Magnetic Resonance Imaging and Clinical Outcomes

Medical Science Monitor ◽

10.12659/msm.901864 ◽

2017 ◽

Vol 23 ◽

pp. 2745-2750 ◽

Cited By ~ 9

Author(s):

Jun Xia ◽

Anyu Yin ◽

Zhenzhou Li ◽

Xin Liu ◽

Xianghong Peng ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Quantitative Analysis ◽

Magnetic Resonance ◽

Clinical Outcomes ◽

Necrotic Core ◽

Atherosclerotic Plaques ◽

Resonance Imaging

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Visualization of Inflammation at Early Stage of Lung Cancer in Xenografted Temporally Immunosuppression Rats by Ferrioxamine Magnetic Resonance Imaging

International Journal of Molecular Imaging ◽

10.1155/2016/8434308 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9

Author(s):

Nathupakorn Dechsupa ◽

Chatchanok Udomtanakunchai ◽

Anan Udom-Utraracheva ◽

Dutsadee Suttho ◽

Lionel Pazart ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Chronic Inflammation ◽

Early Stage ◽

Small Cell Lung Carcinoma ◽

Molecular Probe ◽

Resonance Imaging ◽

Cell Lung Carcinoma ◽

Imaging Instrument

Physiological responses such as chronic inflammation and angiogenesis could be used as biomarkers for early detection of cancer with noninvasive imaging modalities. The present study reports the application of magnetic resonance imaging instrument to image the binding of ferrioxamine with hemin that allows visualizing the chronic inflammation foci of lung tissue of immunocompromised rats xenografted using small cell lung carcinoma. A low concentration of ferrioxamine (0.05±0.02 μM·kg−1 of rat weight) deposited on tissue outside the vasculature was found to diffuse across the capillary walls to the interstitial space and inflammation foci, which provided a clear enhancement of T1-weighted gradient-echo sequence images. Ferrioxamine imaging allowed the determination of inflammatory sites and their localization in 3D fat-suppressed maximum intensity projections. The smallest dimension of foci that can be clearly determined is about 0.1 mm3. In concomitant to the in vivo imaging, analysis of histological tissue section showed the development of inflammatory sites. This study provides evidence that medical imaging instrument such as MRI scanner allows researchers to correlate images taken with MRI with those using high-resolution microscopy. Moreover, ferrioxamine is a useful molecular probe for determining chronic inflammation particularly at the very early stages of cancer.

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Asialoglycoprotein receptor targeted optical and magnetic resonance imaging and therapy of liver fibrosis using pullulan stabilized multi-functional iron oxide nanoprobe

Scientific Reports ◽

10.1038/s41598-021-97808-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Ariya Saraswathy ◽

Shaiju S. Nazeer ◽

Nirmala Nimi ◽

Hema Santhakumar ◽

Parvathy Radhakrishnapillai Suma ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Iron Oxide ◽

Magnetic Resonance ◽

Liver Fibrosis ◽

Early Stage ◽

Iron Oxide Nanoparticle ◽

Dual Function ◽

Relaxation Rates ◽

Resonance Imaging

AbstractEarly diagnosis and therapy of liver fibrosis is of utmost importance, especially considering the increased incidence of alcoholic and non-alcoholic liver syndromes. In this work, a systematic study is reported to develop a dual function and biocompatible nanoprobe for liver specific diagnostic and therapeutic applications. A polysaccharide polymer, pullulan stabilized iron oxide nanoparticle (P-SPIONs) enabled high liver specificity via asialogycoprotein receptor mediation. Longitudinal and transverse magnetic relaxation rates of 2.15 and 146.91 mM−1 s−1 respectively and a size of 12 nm, confirmed the T2 weighted magnetic resonance imaging (MRI) efficacy of P-SPIONs. A current of 400A on 5 mg/ml of P-SPIONs raised the temperature above 50 °C, to facilitate effective hyperthermia. Finally, a NIR dye conjugation facilitated targeted dual imaging in liver fibrosis models, in vivo, with favourable histopathological results and recommends its use in early stage diagnosis using MRI and optical imaging, and subsequent therapy using hyperthermia.

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