The challenges of screening large populations

Menopause ◽  
2022 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Annekathryn Goodman
Keyword(s):  
Large Populations

Morphogenetic machines revealed: Microscopy of living cells in the embryo of the frog, Xenopus laevis

1993 ◽  
Vol 51 ◽  
pp. 172-173
Author(s):  
Ray Keller
Keyword(s):  
Image Processing ◽  
Fluorescent Dyes ◽  
Cell Movement ◽  
Living Cells ◽  
Ease Of Use ◽  
Low Light ◽  
Amphibian Embryo ◽  
Large Populations ◽  
Light Fluorescence ◽  
Video Image Processing

The amphibian embryo offers advantages of size, availability, and ease of use with both microsurgical and molecular methods in the analysis of fundamental developmental and cell biological problems. However, conventional wisdom holds that the opacity of this embryo limits the use of methods in optical microscopy to resolve the cell motility underlying the major shape-generating processes in early development.These difficulties have been circumvented by refining and adapting several methods. First, methods of explanting and culturing tissues were developed that expose the deep, nonepithelial cells, as well as the superficial epithelial cells, to the view of the microscope. Second, low angle epi-illumination with video image processing and recording was used to follow patterns of cell movement in large populations of cells. Lastly, cells were labeled with vital, fluorescent dyes, and their behavior recorded, using low-light, fluorescence microscopy and image processing. Using these methods, the details of the cellular protrusive activity that drives the powerful convergence (narrowing)

The Use of Medical Record Linkage for Population and Genetic Studies

1969 ◽  
Vol 08 (01) ◽  
pp. 07-11 ◽  
Author(s):  
H. B. Newcombe
Keyword(s):  
Record Linkage ◽  
Large Scale ◽  
Medical Record Linkage ◽  
Canadian Province ◽  
Genetic Studies ◽  
Parental Characteristics ◽  
Family Histories ◽  
The Family ◽  
Large Populations ◽  
Machine Readable

Methods are described for deriving personal and family histories of birth, marriage, procreation, ill health and death, for large populations, from existing civil registrations of vital events and the routine records of ill health. Computers have been used to group together and »link« the separately derived records pertaining to successive events in the lives of the same individuals and families, rapidly and on a large scale. Most of the records employed are already available as machine readable punchcards and magnetic tapes, for statistical and administrative purposes, and only minor modifications have been made to the manner in which these are produced.As applied to the population of the Canadian province of British Columbia (currently about 2 million people) these methods have already yielded substantial information on the risks of disease: a) in the population, b) in relation to various parental characteristics, and c) as correlated with previous occurrences in the family histories.

An Enzyme Immunoassay (ELISA) for the Quantitation of Human Factor VII

1986 ◽  
Vol 56 (03) ◽  
pp. 250-255 ◽  
Author(s):  
C Boyer ◽  
M Wolf ◽  
C Rothschild ◽  
M Migaud ◽  
J Amiral ◽  
...  
Keyword(s):  
Human Factor ◽  
Solid Phase ◽  
Factor Vii ◽  
Enzyme Linked Immunosorbent Assay ◽  
Poor Correlation ◽  
Vein Thrombosis ◽  
Coagulant Activity ◽  
Significant Difference ◽  
Large Populations ◽  
Deep Vein

SummaryA new solid phase enzyme-linked immunosorbent assay (ELISA) was developed for the quantitation of human Factor VII antigen (F VII Ag), using a monospecific rabbit anti-F VII antiserum. Anti-F VII F(ab′)2 fragments were adsorbed to polystyrene plates. The binding of serial dilutions of control or test plasma, containing F VII, was detected by incubation with peroxidase-labeled anti- FV II IgG followed by the addition of hydrogen peroxyde and O-phenylenediamine. This ELISA is specific, sensitive (detection limit: 0.05%) and accurate (coefficient of variation: 1.5-4% for within- and 1.6-9% for between-assays). F VII coagulant activity (F VII C) and F VII Ag were determined in large populations of controls and patients. In normal plasma (n = 38), F VII Ag ranged from 83 to 117% and the correlation coefficient between F VII Ag and F VII C was 0.94. In patients with severe (F VII C inf. 1%) congenital F VII deficiency (n = 5), F VII Ag was undetectable in two cases (inf. 0.05%) and markedly reduced (0.35 to 5.6%) in the three other cases. In patients with liver cirrhosis (n = 15), F VII Ag ranged from 21 to 59% and was in good correlation with F VII C (r = 0.84). In dicoumarol treated patients (n = 15), the levels of F VII Ag ranged from 51% to 79% and a poor correlation (r = 0.52) with F VIIC was observed. In “compensated” DIC (n = 5), levels of F VII Ag varied from 60 to 186%, with significantly higher F VII C levels (from 143 to 189%). In contrast, in “decompensated” DIC (n = 7), low F VII Ag and F VII C levels were observed (from 7 to 27%). In patients with deep-vein thrombosis (n = 25), high levels of F VII Ag (from 102 to 136%) and F VII C (from 110 to 150%) were demonstrated. In surgical patients, no significant difference was observed before and one day after intervention.

Galectin-3 in Heart Failure – Linking Fibrosis, Remodelling and Progression

2010 ◽  
Vol 6 (2) ◽  
pp. 33 ◽  
Author(s):  
Christopher R deFilippi ◽  
G Michael Felker ◽  
◽  
Keyword(s):  
Heart Failure ◽  
Ejection Fraction ◽  
Risk Stratification ◽  
Cardiac Fibrosis ◽  
The Elderly ◽  
Preserved Ejection Fraction ◽  
Heart Failure Patients ◽  
Large Populations ◽  
Galectin 3

For many with heart failure, including the elderly and those with a preserved ejection fraction, both risk stratification and treatment are challenging. For these large populations and others there is increasing recognition of the role of cardiac fibrosis in the pathophysiology of heart failure. Galectin-3 is a novel biomarker of fibrosis and cardiac remodelling that represents an intriguing link between inflammation and fibrosis. In this article we review the biology of galectin-3, recent clinical research and its application in the management of heart failure patients.

Consular Protection and Solidarity across Borders

2018 ◽  
Author(s):  
Alexandra Délano Alonso
Keyword(s):  
United States ◽  
Latin American ◽  
Legal Status ◽  
Well Being ◽  
The United States ◽  
Migrant Populations ◽  
Policy Goals ◽  
Large Populations ◽  
Mexican Government ◽  
Working Together

This chapter demonstrates how Latin American governments with large populations of migrants with precarious legal status in the United States are working together to promote policies focusing on their well-being and integration. It identifies the context in which these processes of policy diffusion and collaboration have taken place as well as their limitations. Notwithstanding the differences in capacities and motivations based on the domestic political and economic contexts, there is a convergence of practices and policies of diaspora engagement among Latin American countries driven by the common challenges faced by their migrant populations in the United States and by the Latino population more generally. These policies, framed as an issue of rights protection and the promotion of migrants’ well-being, are presented as a form of regional solidarity and unity, and are also mobilized by the Mexican government as a political instrument serving its foreign policy goals.

Accessible Elections

2020 ◽  
Author(s):  
Michael Ritter ◽  
Caroline J. Tolbert
Keyword(s):  
Voter Turnout ◽  
Population Data ◽  
State Governments ◽  
Public Officials ◽  
Early Voting ◽  
Beneficial Effects ◽  
Election Reform ◽  
Individual Vote ◽  
Large Populations ◽  
Voting Methods

This book explores the wide variation across states in convenience voting methods—absentee/mail voting, in-person early voting, same day registration—and provides new empirical analysis of the beneficial effects of these policies, not only in increasing voter turnout overall, but for disadvantaged groups. By measuring both convenience methods and implementation of the laws, the book improves on previous research. It draws generalizable conclusions about how these laws affect voter turnout by using population data from the fifty state voter files. Using individual vote histories, the design helps avoid bias in non-random assignment of states in adopting the laws. Many scholars and public officials have dismissed state election reform laws as failing to significantly increase turnout or address inequality in who votes. Accessible Elections underscores how state governments can modernize their election procedures to increase voter turnout and influence campaign and party mobilization strategies. Mail voting and in-person early voting are particularly important in the wake of Covid-19 to avoid election day crowds and ensure successful and equitable elections in states with large populations; the results of this study can help state governments more rapidly update voting for the 2020 general election and beyond.

Outbreeding depression is uncommon and predictable

2017 ◽  
Author(s):  
Richard Frankham ◽  
Jonathan D. Ballou ◽  
Katherine Ralls ◽  
Mark D. B. Eldridge ◽  
Michele R. Dudash ◽  
...  
Keyword(s):  
Natural Selection ◽  
Genetic Differences ◽  
Outbreeding Depression ◽  
Isolated Populations ◽  
Large Populations

Crosses between populations within species sometimes result in reduced fitness, especially in F2 and later generations (outbreeding depression). The primary mechanisms causing outbreeding depression in crosses between populations are fixed chromosomal differences and adaptive genetic differences, especially for long-isolated populations. Outbreeding depression is usually observed after crossing populations with ploidy differences or fixed differences for translocations, inversions or centric fusions: the magnitudes are usually ploidy > translocations and monobrachial centric fusions > inversions and simple centric fusions. Populations adapted to different environments (but with the same karyotype) often exhibit outbreeding depression when crossed, especially in the F2 and later generations. Even if outbreeding depression occurs, it is often only temporary, as natural selection acts to remove it, especially in large populations.

Transmission of SARS-CoV-2 in Different Districts of a County in Istanbul, March to September 2020

2021 ◽  
pp. 101053952110090
Author(s):  
Hüseyin Bilgin ◽  
Ahmet Topuzoğlu ◽  
Volkan Korten
Keyword(s):  
Public Transportation ◽  
Disease Transmission ◽  
Geographical Information Systems ◽  
Geographical Information ◽  
Chain Reaction ◽  
Heat Map ◽  
Large Populations ◽  
Polymerase Chain ◽  
Education Tool ◽  
Airborne Viruses

Epidemics caused by airborne viruses in cities with large populations create a big problem as in the current COVID-19 pandemic. Cramped lifestyle, busy workplaces, crowded public transportation, and higher household member counts are responsible for the transmission of the disease. In Turkey, Istanbul has taken the lead in the number of cases since the beginning of the epidemic. The excess population density is the major cause for disease transmission. It is essential to monitor the contaminated regions with geographical information systems on city maps. Outbreak maps visualize and help analyze the patterns of transmission and serve as a communication and education tool. A dynamic heat map video of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) polymerase chain reaction positive cases in a county of Istanbul was generated. The heat map visualizes how the epidemic spread to all the districts and the cumulative cases increased in one county of Istanbul with real attack rates.

scholarly journals Autosomal Microsatellite Investigation Reveals Multiple Genetic Components of the Highlanders from Thailand

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 383
Author(s):  
Aornpriya Mawan ◽  
Nonglak Prakhun ◽  
Kanha Muisuk ◽  
Suparat Srithawong ◽  
Metawee Srikummool ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Genetic Relationships ◽  
Forensic Investigation ◽  
Northern Thailand ◽  
Hill Tribe ◽  
Genetic Components ◽  
Large Populations ◽  
Population Interactions ◽  
Hill Tribes ◽  
The Hill

The hill tribes of northern Thailand comprise nine officially recognized groups: the Austroasiatic-speaking (AA) Khmu, Htin and Lawa; the Hmong-Mien-speaking (HM) IuMien and Hmong; and the Sino-Tibetan-speaking (ST) Akha, Karen, Lahu and Lisu. Except the Lawa, the rest of the hill tribes migrated into their present habitats only very recently. The Thai hill tribes were of much interest to research groups focusing on study of cultural and genetic variation because of their unique languages and cultures. So far, there have been several genetic studies of the Thai hill tribes. However, complete forensic microsatellite database of the Thai hill tribes is still lacking. To construct such database, we newly generated 654 genotypes of 15 microsatellites commonly used in forensic investigation that belong to all the nine hill tribes and also non-hill tribe highlanders from northern Thailand. We also combined 329 genotypes from previous studies of northern Thai populations bringing to a total of 983 genotypes, which were then subjected to genetic structure and population relationships analyses. Our overall results indicated homogenous genetic structure within the HM- and Tai-Kadai (TK)-speaking groups, large genetic divergence of the HM-speaking Hmong but not IuMien from the other Thai groups, and genetic heterogeneity within the ST- and AA-speaking groups, reflecting different population interactions and admixtures. In addition to establishing genetic relationships within and among these populations, our finding, which provides a more complete picture of the forensic microsatellite database of the multiple Thai highland dwellers, would not only serve to expand and strengthen forensic investigation in Thailand, but would also benefit its neighboring countries of Laos and Myanmar, from which many of the Thai hill tribes originated and where large populations of these ethnic groups still reside.

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