scholarly journals Direct and trans-generational effects of male and female gut microbiota in Drosophila melanogaster

2017 ◽  
Vol 13 (7) ◽  
pp. 20160966 ◽  
Author(s):  
Juliano Morimoto ◽  
Stephen J. Simpson ◽  
Fleur Ponton
Keyword(s):  
Drosophila Melanogaster ◽  
Gut Microbiota ◽  
Male Fertility ◽  
Gut Bacteria ◽  
Short Term ◽  
Male And Female ◽  
Offspring Production ◽  
Generational Effects ◽  
Negative Effect ◽  
All Treatment

There is increasing evidence of the far-reaching effects of gut bacteria on physiological and behavioural traits, yet the fitness-related consequences of changes in the gut bacteria composition of sexually interacting individuals remain unknown. To address this question, we manipulated the gut microbiota of fruit flies, Drosophila melanogaster , by monoinfecting flies with either Acetobacter pomorum ( AP ) or Lactobacillus plantarum ( LP ) . Re-inoculated individuals were paired in all treatment combinations. LP- infected males had longer mating duration and induced higher short-term offspring production in females compared with AP -infected males. Furthermore, females of either re-inoculation state mated with AP- infected males were more likely to have zero offspring after mating, suggesting a negative effect of AP on male fertility . Finally, we found that the effects of male and female gut bacteria interacted to modulate their daughters', but not sons' body mass, revealing a new trans-generational effect of parental gut microbiota. In conclusion, this study shows direct and trans-generational effects of the gut microbiota on mating and reproduction.

scholarly journals Drosophila melanogasterestablishes a species-specific mutualistic interaction with stable gut-colonizing bacteria

2018 ◽  
Author(s):  
Inês S. Pais ◽  
Rita S. Valente ◽  
Marta Sporniak ◽  
Luis Teixeira
Keyword(s):  
Drosophila Melanogaster ◽  
Gut Microbiota ◽  
Bacterial Communities ◽  
Bacterial Species ◽  
Gut Bacteria ◽  
Natural Food ◽  
Strong Impact ◽  
Next Generation ◽  
Stable Association ◽  
Host Development

AbstractAnimals live together with diverse bacteria that can impact their biology. InDrosophila melanogaster, gut-associated bacterial communities are relatively simple in composition but also have a strong impact on host development and physiology. However, it is still unknown if bacteria can proliferate and stably associate with the gut ofD. melanogaster. In fact, it is generally assumed that bacteria are transient and their constant ingestion with food is required to maintain their presence in the gut. Here, we identify bacterial species from wild-caughtD. melanogasterthat stably associate with the host independently of continuous inoculation. Moreover, we show that specificAcetobacterwild isolates can proliferate in the gut. We further demonstrate that the interaction betweenD. melanogasterand the wild isolatedAcetobacter thailandicusis mutually beneficial and that the stability of the gut association is key to this mutualism. The stable population in the gut ofD. melanogasterallows continuous bacterial spreading into the environment, which is advantageous to the bacterium itself. The bacterial dissemination is in turn advantageous to the host since the next generation of flies develops in the presence of this particularly beneficial bacterium.Ac. thailandicusleads to a faster host development and higher fertility of emerging adults, when compared to other bacteria isolated from wild-caught flies. Furthermore,Ac. thailandicusis sufficient and advantageous whenD. melanogasterdevelops in axenic or freshly collected figs, respectively. This isolate ofAc. thailandicuscolonizes several genotypes ofD. melanogasterbut not of the closely relatedDrosophila simulans, indicating that the stable association is host specific. This work establishes a new conceptual model to understandD. melanogaster- gut microbiota interactions in an ecological context; stable interactions can be mutualistic through microbial farming, a common strategy in insects. Moreover, these results develop the use ofD. melanogasteras a model to study gut microbiota proliferation and colonization.Author summaryAnimals, including humans, live together with complex bacterial communities in their gut that influence their physiology and health. The fruit flyDrosophila melanogasterhas been an excellent model organism to study host-microbe interactions and harbours a relative simple gut bacterial community. However, it is not known which of these bacteria can proliferate and form stable communities in the gut, and the current hypothesis is that these bacteria are only transiently associated with the gut. Here, we show that inD. melanogastercollected from a natural population stable gut bacteria do exist. We isolated specific species that can proliferate in the gut and form a stable association. This is beneficial to the bacteria since they can be constantly spread by the flies as they move around. On the other hand, this is a form of farming as the next generation of flies benefit from the association with these particular bacteria during development. They become adults faster and are more fertile than if they develop with other bacteria encountered in nature. These advantages are also observed when flies develop in figs, a natural food source. Our findings show thatD. melanogasterhas stable colonizing bacteria in the gut and establish a new framework to study host-gut bacteria interactions.

scholarly journals GENETIC ANALYSIS OF CHROMOMERE 3D4 IN DROSOPHILA MELANOGASTER: THE DUNCE AND SPERM-AMOTILE GENES

Genetics ◽  
1982 ◽  
Vol 100 (4) ◽  
pp. 587-596
Author(s):  
Helen K Salz ◽  
Ronald L Davis ◽  
John A Kiger
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Analysis ◽  
Male Fertility ◽  
Genetic Background ◽  
Female Fertility ◽  
Phosphodiesterase Activity ◽  
Male And Female ◽  
Chromosomal Break ◽  
Gene Maps

ABSTRACT Both male and female Drosophila that are homozygous deficient for chromomere 3D4 are viable but sterile and lack detectable cAMP-specific phosphodiesterase activity. Two genes have been localized to this region: spermamotile (sam) and dunce (dnc). The sperm-amotile gene is required for male fertility, and the dunce gene is required for normal learning, female fertility, and cAMP-specific phosphodiesterase activity. The sperm-amotile gene maps 0.24 map units to the left of dunce. The expression of the dunce gene seems to be affected by a chromosomal break to the left of sperm-amotile. The fertility of dunce females varies according to changes in the genetic background and the presence or absence of an X-linked suppressor.

The mechanisms involved in obesity-induced male infertility

2020 ◽  
Vol 16 ◽  
Author(s):  
Hamed Heydari ◽  
Rafighe Ghiasi ◽  
Saber Ghaderpour ◽  
Rana Keyhanmanesh
Keyword(s):  
Oxidative Stress ◽  
Metabolic Disease ◽  
Male Infertility ◽  
Male Fertility ◽  
Reproductive Function ◽  
Disease Development ◽  
Significant Evidence ◽  
Male Reproductive Function ◽  
Negative Effect ◽  
And Oxidative Stress

Introduction: Obesity resulted by imbalance between the intake of energy and energy consumption can lead to growth and metabolic disease development in people. Both in obese men and animal models, several studies indicate that obesity leads to male infertility. Objective: This review has discussed some mechanisms involved in obesity-induced male infertility. Method: Online documents were searched through Science Direct, Pubmed, Scopus, and Google Scholar websites dating from 1959 to recognize studies on obesity, kisspeptin, leptin, and infertility. Results: Obesity induced elevated inflammatory cytokines and oxidative stress can affect male reproductive functions including spermatogenesis disorders, reduced male fertility power and hormones involved in hypothalamus-pituitarygonadal axis. Conclusion: There is significant evidence that obesity resulted in male infertility. obesity has negative effect on male reproductive function via several mechanisms such as inflammation and oxidative stress.

scholarly journals FRI0632-HPR PSYCHOLOGICAL PARTICULIARITIES OF PATIENTS SUFFERING FROM MONOGENIC AUTO-INFLAMMATORY DISEASES.

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 920.1-921
Author(s):  
N. Stepanenko ◽  
E. Fedorov ◽  
S. Salugina ◽  
S. Feoktistova
Keyword(s):  
Inflammatory Diseases ◽  
Life Quality ◽  
Information Storage ◽  
Social Adaptation ◽  
Long Term Memory ◽  
Short Term ◽  
Sequence Of Events ◽  
Communicative Disorders ◽  
Negative Effect ◽  
High Level

Background:Monogenic auto-inflammatory diseases (mAID) are a group of severe chronic multisystemic diseases with recurring episodes of fever and other manifestations that significantly affect the patients’ life quality. Moreover, the hyper expression of pro-inflammatory cytokines (IL1β, etc.) observed in these patients may have a negative effect on the central nervous system.Objectives:to study the state of the cognitive and emotional spheres in children suffering from monogenic auto-inflammatory diseases.Methods:there were examined 22 children at the age of 7 to 17 years old diagnosed with CAPS-9, TRAPS-8, FMF-5. Among them there were 12 boys and 10 girls. The diagnosis in all the patients was confirmed through detection of pathogenic mutations in the NLRP3, TNFRSF1A and MEFV genes. The following methods were used: a clinical conversation; memory diagnostics (learning by heart of 10 words, a pictogram using cues taking into account the patients’ age); attention diagnostics (Schulte tables); thinking diagnostics (establishing a sequence of events, “four is a crwod”, simple analogies, interpretation of proverbs); emotional and communicative fields (the Eight-Color Luscher Test; CMAS (adaptation by A. Prikhozhan); STAI test, a drawing called “an animal that does not exist” and “a house-a tree-a man”).Results:The memory study revealed in all patients with TRAPS and FMF high and medium values of short-term and long-term memory, in patients with CAPS - a low level of short-term auditory-speech memory, information storage and indirect memorization in 1/3 of patients. In 100% of the examined patients with TRAPS, a significant decrease in all processes of attention and distribution of attention. In 1/3 of patients with CAPS, an increased exhaustion of attention was registered and in 11% - a decrease in its stability. In patients with FMF, attention disorders were not detected. In 44% of patients with CAPS, a decrease in the level of generalization and difficulties in establishing causal relationships were registered. In 25% of patients with TRAPS a decrease in the level of generalization, in 12.5%- difficulties in establishing cause-effect relationships, inertia of thinking in 37.5%. In 60% of patients with FMF: a decrease in the level of generalization, in 80%: difficulties in establishing cause-effect relationships, inertia of thinking in 20%. In the emotional sphere, patients with CAPS, TRAPS, and FMF demonstrated signs of aggression (11.1%, 20% and 20% of patients, respectively), communicative disorders (77.8% -80% - 80%), and reduced social adaptation (55.5% - 80% - 80%), a tendency to form neurotic fears (22% - 40% - 40%). A high level of personal anxiety was noted in 1/3 of patients with CAPS and 40% of patients with FMF.Conclusion:various psychological disorders in the cognitive and emotional fields were noted in the majority of the examined patients with monogenic auto-inflammatory diseases. In patients with TRAPS, attention processes are most significantly affected; in patients with CAPS, memory is more often affected. In patients with FMF, disorders in thinking processes are revealed more often. In the emotional sphere, most patients with all the three forms of AID note communicative disorders and social adaptation.Disclosure of Interests:None declared

scholarly journals Gut microbiome diversity is an independent predictor of survival in cervical cancer patients receiving chemoradiation

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Travis T. Sims ◽  
Molly B. El Alam ◽  
Tatiana V. Karpinets ◽  
Stephanie Dorta-Estremera ◽  
Venkatesh L. Hegde ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Radiation Therapy ◽  
Gut Microbiota ◽  
Cancer Patients ◽  
Gut Microbiome ◽  
Compositional Variation ◽  
Short Term ◽  
Microbiome Diversity ◽  
Term Survivor

AbstractDiversity of the gut microbiome is associated with higher response rates for cancer patients receiving immunotherapy but has not been investigated in patients receiving radiation therapy. Additionally, current studies investigating the gut microbiome and outcomes in cancer patients may not have adjusted for established risk factors. Here, we sought to determine if diversity and composition of the gut microbiome was independently associated with survival in cervical cancer patients receiving chemoradiation. Our study demonstrates that the diversity of gut microbiota is associated with a favorable response to chemoradiation. Additionally, compositional variation among patients correlated with short term and long-term survival. Short term survivor fecal samples were significantly enriched in Porphyromonas, Porphyromonadaceae, and Dialister, whereas long term survivor samples were significantly enriched in Escherichia Shigella, Enterobacteriaceae, and Enterobacteriales. Moreover, analysis of immune cells from cervical tumor brush samples by flow cytometry revealed that patients with a high microbiome diversity had increased tumor infiltration of CD4+ lymphocytes as well as activated subsets of CD4 cells expressing ki67+ and CD69+ over the course of radiation therapy. Modulation of the gut microbiota before chemoradiation might provide an alternative way to enhance treatment efficacy and improve treatment outcomes in cervical cancer patients.

scholarly journals Production and scavenging of reactive oxygen species both affect reproductive success in male and female Drosophila melanogaster

2021 ◽  
Author(s):  
Biz R. Turnell ◽  
Luisa Kumpitsch ◽  
Klaus Reinhardt
Keyword(s):  
Reactive Oxygen Species ◽  
Drosophila Melanogaster ◽  
Reactive Oxygen ◽  
Cellular Aging ◽  
Ros Production ◽  
Oxygen Species ◽  
Wild Type ◽  
Ros Scavenging ◽  
Respiratory Pathway ◽  
Male And Female

AbstractSperm aging is accelerated by the buildup of reactive oxygen species (ROS), which cause oxidative damage to various cellular components. Aging can be slowed by limiting the production of mitochondrial ROS and by increasing the production of antioxidants, both of which can be generated in the sperm cell itself or in the surrounding somatic tissues of the male and female reproductive tracts. However, few studies have compared the separate contributions of ROS production and ROS scavenging to sperm aging, or to cellular aging in general. We measured reproductive fitness in two lines of Drosophila melanogaster genetically engineered to (1) produce fewer ROS via expression of alternative oxidase (AOX), an alternative respiratory pathway; or (2) scavenge fewer ROS due to a loss-of-function mutation in the antioxidant gene dj-1β. Wild-type females mated to AOX males had increased fecundity and longer fertility durations, consistent with slower aging in AOX sperm. Contrary to expectations, fitness was not reduced in wild-type females mated to dj-1β males. Fecundity and fertility duration were increased in AOX and decreased in dj-1β females, indicating that female ROS levels may affect aging rates in stored sperm and/or eggs. Finally, we found evidence that accelerated aging in dj-1β sperm may have selected for more frequent mating. Our results help to clarify the relative roles of ROS production and ROS scavenging in the male and female reproductive systems.

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