scholarly journals Surface wettability of plasma SiO x :H nanocoating-induced endothelial cells' migration and the associated FAK-Rho GTPases signalling pathways

2011 ◽  
Vol 9 (67) ◽  
pp. 313-327 ◽  
Author(s):  
Yang Shen ◽  
Guixue Wang ◽  
Xianliang Huang ◽  
Qin Zhang ◽  
Jiang Wu ◽  
...  

Vascular endothelial cell (EC) adhesion and migration are essential processes in re-endothelialization of implanted biomaterials. There is no clear relationship and mechanism between EC adhesion and migration behaviour on surfaces with varying wettabilities. As model substrates, plasma SiO x :H nanocoatings with well-controlled surface wettability (with water contact angles in the range of 98.5 ± 2.3° to 26.3 ± 4.0°) were used in this study to investigate the effects of surface wettability on cell adhesion/migration and associated protein expressions in FAK-Rho GTPases signalling pathways. It was found that EC adhesion/migration showed opposite behaviour on the hydrophilic and hydrophobic surfaces (i.e. hydrophobic surfaces promoted EC migration but were anti-adhesions). The number of adherent ECs showed a maximum on hydrophilic surfaces, while cells adhered to hydrophobic surfaces exhibited a tendency for cell migration. The focal adhesion kinase (FAK) inhibitor targeting the Y-397 site of FAK could significantly inhibit cell adhesion/migration, suggesting that EC adhesion and migration on surfaces with different wettabilities involve ( p )FAK and its downstream signalling pathways. Western blot results suggested that the FAK-Rho GTPases signalling pathways were correlative to EC migration on hydrophobic plasma SiO x :H surfaces, but uncertain to hydrophilic surfaces. This work demonstrated that surface wettability could induce cellular behaviours that were associated with different cellular signalling events.

Oncogene ◽  
2020 ◽  
Vol 39 (18) ◽  
pp. 3666-3679 ◽  
Author(s):  
Mario De Piano ◽  
Valeria Manuelli ◽  
Giorgia Zadra ◽  
Jonathan Otte ◽  
Per-Henrik D. Edqvist ◽  
...  

2012 ◽  
Vol 562-564 ◽  
pp. 56-59 ◽  
Author(s):  
Jian Zhuang ◽  
Meng Meng Du ◽  
Heng Zhi Cai ◽  
Ya Jun Zhang ◽  
Da Ming Wu

A facile method for manufacturing super hydrophobic surfaces is presented using the stainless steel wire mesh as templates. The rough surfaces of polymers including polycarbonate, polypropylene and PMMA are prepared with hot embossing on different specifications of stainless steel wire mesh. Scanning electron microscopy (SEM) results reveal that the surfaces roughness of the polymers can be controlled by selecting templates. Contact angle measurement shows that the water contact angles(WCA) rise with the increase of surface roughness, especially, the water contact angle on the PC surfaces prepared with specifications of 635mesh screen can reach to 152.3°, alias super hydrophobic surfaces.


Author(s):  
Hai Trieu Phan ◽  
Nadia Caney ◽  
Philippe Marty ◽  
Ste´phane Colasson ◽  
Je´roˆme Gavillet

Although boiling process has been a major subject of research for several decades, its physics still remain unclear and require further investigation. This study aims at highlighting the effects of the surface wettability on pool boiling heat transfer. Nanocoating techniques were used to vary the water contact angle from 20 to 110° by modifying nanoscale surface topography and chemistry. The experimental results obtained disagree with the predictions of the classical models. A new approach of nucleation mechanism is established to clarify the nexus between the surface wettability and the nucleate boiling heat transfer. In this approach, we introduce the concept of macro- and micro-contact angles to explain the observed phenomenon.


Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1136 ◽  
Author(s):  
Rok Zaplotnik ◽  
Alenka Vesel

Precise tailoring of surface properties by gaseous plasma treatments remains a key scientific challenge, especially when adequate surface wettability should be laterally distributed, and sharp interfaces between hydrophobic and hydrophilic areas are desirable. The evolution of surface wettability and functional groups on polystyrene (PS) upon treatment with argon plasma jet was monitored by water contact angles and X-ray photoelectron spectroscopy (XPS). An array of water droplets was deposited on PS samples treated either directly by the plasma jet or only VUV radiation arising from the plasma. Rather sharp interfaces between the activated and not-affected regions were observed in both cases. The functionalization with highly-oxidized carbon functional groups, as determined by high-resolution C1s XPS spectra, was by far more efficient using the VUV radiation only. In contrast, the optimal wettability was achieved using direct plasma treatment. The results were explained by different mechanisms involved in the interaction of radiation and reactive plasma species with the polymer surface.


Author(s):  
Hai Trieu Phan ◽  
Nadia Caney ◽  
Philippe Marty ◽  
Stephane Colasson ◽  
Je´roˆme Gavillet ◽  
...  

Although boiling process has been a major subject of research for several decades, its physics still remain unclear and require further investigation. This study aims at highlighting the effects of the surface wettability on pool boiling heat transfer. Nanocoating techniques were used to vary the water contact angle from 20 to 110° by modifying nanoscale surface topography and chemistry. The experimental results obtained disagree with the predictions of the classical models. A new approach of nucleation mechanism is established to clarify the nexus between the surface wettability and the nucleate boiling heat transfer. In this approach, we introduce the concept of macro- and micro-contact angles to explain the observed phenomenon.


Coatings ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 352 ◽  
Author(s):  
Luigi Calabrese ◽  
Amani Khaskhoussi ◽  
Salvatore Patane ◽  
Edoardo Proverbio

Superhydrophobicity is one of the most required surface properties for a wide range of application such as self-cleaning, anti-corrosion, oil-water separation, anti-icing, and anti-bioadhesion. Recently, several methods have been developed to produce nature inspired super-hydrophobic surfaces. Nevertheless, these methods require a complicated process and expensive equipment. In order to overcome these issues, we propose three different methods to obtain nature-inspired super-hydrophobic surfaces: short-term treatment with boiling water, HF/HCl and HNO3/HCl concentrated solution etching. Afterwards, a thin layer of octadecylsilane was applied by in situ polymerization on all pre-treated surfaces. Eventually, all substrates were dried for 3 h at 100 °C to complete the silane curing. Scanning electron microscopy (SEM), contact angle measuring system and atomic force microscope (AFM) were used to characterize the surfaces. Surface morphology analysis showed that each method results in a specific dual hierarchical nano-/micro-structure. The corresponding water contact angles ranged from 160° to nearly 180°. The best results were observed for HF etched Al 6082 surface were water contact angle above 175° was achieved. Furthermore, a scheme able to assess the relationship between hydrophobic behavior and surface morphology was finally proposed.


2021 ◽  
Author(s):  
Bing Wang ◽  
Jiaru Zhang ◽  
Yingchun Guan

Abstract Metallic materials have been widely used owing to their good mechanical property and high flexibility. However, there are certain limitations for practical applications such as low anti-bacterial, cell adhesion, surface wettability and corrosion resistance property. In this paper, laser microprocessing of titanium (Ti) alloy and magnesium (Mg) alloy has been conducted, respectively. The cell adhesion of Ti6Al4V alloy and Mg-Gd-Ca alloy after laser microprocessing has been investigated. The results show that MC3T3-E1 cells have been successfully adhered to the treated surface and optical density are significantly increased due to hybrid micro/nano structure. Anti-bacterial test shows that the anti-bacterial rates against E. coli of laser-treated surface was up to 72%. Meanwhile, water contact angle has been increased from 57.4° to 135.3° indicating the changing of surface wettability from hydrophilic to hydrophobic. Moreover, corrosion test of Mg-Gd-Ca alloy has been conducted, which has been significantly improved after laser microprocessing. The present work showed that laser surface microprocessing could be a promising technique for fabricating different biomedical property surfaces.


Author(s):  
Rodrigo Ferreira ◽  
Gabriel Lopes de Castro Martinelli ◽  
Alessandro Roger Rodrigues ◽  
Reginaldo Teixeira Coelho

Surface texturing, using micro-milling, has promising applications in the industry of medical implants, since it can assist cell adhesion and thus improve osseointegration. Ti6Al4V alloy is used as implant material due to its excellent biocompatibility and high mechanical strength. However, those mechanical properties reduce machinability creating some challenges for micro-milling. The way to initially assess cell adhesion is using surface wettability, usually conducted with water. At the present work, micro-channels were machined in Ti6Al4V by micro end-milling with 500 µm width per 50 µm depth with 1000 µm distant from each other. The effect of feed per tooth (fz) on wettability was analysed and some interesting relations with burrs formed on channel walls were obtained. Values of feed per tooth were 3, 6, 12 and 15 µm. Wettability results showed that slotted surface is more hydrophilic on channel direction, with contact angles around 30° to 43°. In contrast, on the perpendicular direction the surface tends to be hydrophobic with contact angles between 75° and 146°. In addition, contact angle increases (hydrophobic tendency) as feed per tooth increases (along with roughness), even on channel direction. The presence of burrs also tends to disturb wettability results. Therefore, surface wettability depends on channel direction, burr size and tool feed per tooth, as well.


2011 ◽  
Vol 117-119 ◽  
pp. 1199-1202
Author(s):  
Yong Zhang ◽  
Xiu Fang Wen ◽  
Pi Hui Pi ◽  
Da Feng Zheng ◽  
Zhi Qi Cai ◽  
...  

The relationships between (meth)acrylate polymers structure and its film surface wettability were analyzed. A series of random (meth)acrylate polymers were prepared by the free radical solution polymerization and their coating film wettability was analyzed by measuring water contact angles(CAs). There are three kinds of functional groups on the surface, including methyl, ethyl and ester group, and the surface energy of ester group is greater than the others. The more ester group on the surface will result in the better surface hydrophilicity. Polymer structure parameters as the side chain length(m) and glass temperature(Tg) are taken into account in discussing the migration of functional group to the outermost surface. It was found that the less m and lower Tg will promote the motion of the side chain and hence cause the surface exhibiting more hydrophilicity.


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