Disposable Stainless-Steel Wire-Based Electrochemical Microsensor for In Vivo Continuous Monitoring of Hydrogen Peroxide in Vein of Tomato Leaf

As one of the pivotal signal molecules, hydrogen peroxide (H2O2) has been demonstrated to play important roles in many physiological processes of plants. Continuous monitoring of H2O2 in vivo could help understand its regulation mechanism more clearly. In this study, a disposable electrochemical microsensor for H2O2 was developed. This microsensor consists of three parts: low-cost stainless-steel wire with a diameter of 0.1 mm modified by gold nanoparticles (disposable working electrode), an untreated platinum wire with a diameter of 0.1 mm (counter electrode), and an Ag/AgCl wire with a diameter of 0.1 mm (reference electrode), respectively. The microsensor could detect H2O2 in levels from 10 to 1000 µM and exhibited excellent selectivity. On this basis, the dynamic change in H2O2 in the vein of tomato leaf under high salinity was continuously monitored in vivo. The results showed that the production of H2O2 could be induced by high salinity within two hours. This study suggests that the disposable electrochemical microsensor not only suits continuously detecting H2O2 in microscopic plant tissue in vivo but also reduces the damage to plants. Overall, our strategy will help to pave the foundation for further investigation of the generation, transportation, and elimination mechanism of H2O2 in plants.

Effect of drawing and annealing on the microstructure and mechanical properties of 304 austenitic stainless steel wire

Plastic deformation at room temperature, and the proceeding heat treatments, are important processes for optimizing the microstructure and mechanical properties of austenitic stainless steel. The microstructure and mechanical properties of cold-drawn 304 austenitic stainless steel wire were investigated after annealing at 700 and 800 °C, with different times (20, 40 and 60 min) and drawing strain (0.4, 1.0 and 1.5). Electron backscattered diffraction (EBSD) techniques, trans-mission electron microscope (TEM) analysis, differential scanning calorimeter (DSC) and tensile tests were performed in order to study the microstructure evolution and mechanical properties during different annealing processes for the 304 austenitic stainless steel wire. The results showed that the quantity of α’ martensite and dislocations increased with an increase in the strain, which means that, while the ultimate tensile strength of the cold-drawn wires elevated, the elongation reduced. The mechanical properties of stainless steel wires also varied with the evolution of martensite transformation characteristics, density of stacking fault, dislocation and twin, as well as the recrystallization degree under various annealing conditions. The recrystalli-zation temperature of steel wire was mainly determined by the magnitude of the strain, while the martensite reversal temperature was determined by the stacking fault energy and the de-formation value. The temperature of recrystallization and martensite reverse in steel wire de-creased with the increment of the strain. The balance of tensile strength and elongation of steel wire can be obtained by adopting the proper annealing process combined with cold-drawing deformation. In this paper, we showed that a good combination of strength and elongation in 304 austenitic stainless steel can be obtained with a strain of 1.5 annealed at 800 °C for 20 min.

Rehabilitation and Esthetic Enhancement of Edentulous Patient with Hollow Cheeks Using Innovative Detachable Cheek Plumper: Case Report

Complete edentulism for longer period of time and aging causes flaccid of facial musculature which leads to sunken cheeks and unpleasing appearance of face. In order to get a supplemental support for the drooping cheek muscles, fabrication of conventional complete denture with adjunct detachable cheek plumpers are simple, acceptable non-invasive and economical. Despite the discussion of various attachment used for cheek plumper in literature each have their own disadvantages. The present clinical report demonstrated the use of chairside available lingual sheath and stainless steel wire for the attachment of cheek plumpers to the maxillary complete denture.

Tensile Behavior of High-Strength Stainless Steel Wire Rope (HSSSWR)-Reinforced ECC

Engineered cementitious composites (ECC) show the distinguished characteristics of high post-cracking resistance and ductility. High-strength stainless steel wire rope (HSSSWR) has been successfully used for restoring or strengthening of existing structures. By combining the advantages of these two materials, a new composite system formed by embedding HSSSWR into ECC was proposed and expected to be a promising engineering material for repair or strengthening of structures. To investigate the tensile failure mechanism and mechanical properties of HSSSWR-reinforced ECC, an experimental study on 27 HSSSWR-reinforced ECC plates was conducted considering the effects of the reinforcement ratio of longitudinal HSSSWRs, formula of ECC and width of the plate. Test results revealed that HSSSWR-reinforced ECC exhibit superior post-cracking resistance, deformation capacity and crack-width control capacity. Increasing the reinforcement ratio of longitudinal HSSSWRs can effectively enhance the tensile strength, crack-width control capacity, deformation capacity and tensile toughness of HSSSWR-reinforced ECC. Adding thickener in ECC can significantly improve the crack-width control capacity and deformation capacity of HSSSWR-reinforced ECC due to enhancing uniform distribution of polyvinyl alcohol fibers, but would slightly reduce the cracking stress and maximum tensile stress by bringing small bubbles in the matrix. The tensile properties of HSSSWR-reinforced ECC plates are almost not affected by varying the plate width. Besides, a tensile constitutive model was developed for charactering the stress–strain relationship of HSSSWR-reinforced ECC in tension. Based on mechanical theories and failure characteristics of HSSSWR-reinforced ECC, the model parameters were determined, and calculation equations of cracking stress and tensile strength were proposed. The accuracy of the developed model and calculation equations was verified by test results.