scholarly journals Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil

2019 ◽  
Vol 6 (11) ◽  
pp. 190562 ◽  
Author(s):  
Qingming Zhang ◽  
Hongyu Liu ◽  
Muhammad Saleem ◽  
Caixia Wang
Keyword(s):  
Soil Microorganisms ◽  
Cyano Group ◽  
Gene Sequencing ◽  
Contaminated Sites ◽  
Optimum Conditions ◽  
Metabolic Potential ◽  
16S Rdna Gene ◽  
Farmland Soil ◽  
Chloro Group ◽  
16S Rdna Gene Sequencing

Chlorothalonil is a widely used fungicide, but the contamination of soil and water environments by this chemical causes potential threats to biodiversity. Given the metabolic potential of soil microorganisms, there is a need for developing microbiological approaches to degrade persistent compounds, such as chlorothalonil, in contaminated sites. Here in this study, we isolated a bacterial strain (namely, BJ1) capable of degrading chlorothalonil from a chlorothalonil-contaminated farmland soil in the Shandong Province, China. Using 16S rDNA gene sequencing, morphological and biological characteristics, we identified the strain BJ1 as Stenotrophomonas acidaminiphila . The strain BJ1 uses chlorothalonil as a sole carbon source. At initial concentrations of 50, 100, 200 and 300 mg l −1 , it degraded 91.5%, 89.4%, 86.5% and 83.5% of chlorothalonil after 96 h of inoculation under optimum conditions (30°C and pH 7.0). Two metabolites, methyl-2,5,6-trichloro-3-cyano-4-methoxy-benzoate and methyl-3-cyano-2,4,5,6-tetrachlorobenzoate, were detected and identified based on HPLC–MS analysis, which suggests that the strain BJ1 metabolized chlorothalonil through the hydroxylation of chloro-group and hydration of cyano-group. The results of this study highlight the great potential for this bacterium to be used in chlorothalonil pollution remediation.

scholarly journals Nocardia farcinica - a significant cause of mastitis in goats in Sudan : short communication

2004 ◽  
Vol 75 (3) ◽  
Author(s):  
L.A. Maldonado ◽  
M.E. Hamid ◽  
O.A. Gamal El Din ◽  
M. Goodfellow
Keyword(s):  
16S Rdna ◽  
Short Communication ◽  
Gene Sequencing ◽  
Isolation And Identification ◽  
16S Rdna Gene ◽  
Identification Methods ◽  
Milk Samples ◽  
Nocardia Farcinica ◽  
Mastitic Milk ◽  
16S Rdna Gene Sequencing

Fifteen of 100 mastitic milk samples from goats suffering from mastitis were tentatively identified as members of the genus Nocardia on the basis of selected phenotypic and chemotaxonomic characteristics. Six of the 15 strains were confirmed as Nocardia farcinica by 16S rDNA gene sequencing and subsequent aligning with relevant actinomycetes found in electronic databases and 2 by other identification criteria. N. farcinica is a serious cause of mastitis with a significant prevalence (15%) among the examined goats. Efforts are needed to optimise and simplify isolation and identification methods.

scholarly journals Selection of Autochthonous LAB Strains of Unripe Green Tomato towards the Production of Highly Nutritious Lacto-Fermented Ingredients

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2916
Author(s):  
Nelson Pereira ◽  
Carla Alegria ◽  
Cristina Aleixo ◽  
Paula Martins ◽  
Elsa M. Gonçalves ◽  
...  
Keyword(s):  
Gene Sequencing ◽  
Starter Cultures ◽  
Fermented Foods ◽  
16S Rdna Gene ◽  
Food Ingredients ◽  
Sensory Acceptability ◽  
Probiotic Potential ◽  
Selection Of ◽  
16S Rdna Gene Sequencing

Lactic fermentation of unripe green tomatoes as a tool to produce food ingredients is a viable alternative for adding value to industrial tomatoes unsuitable for processing and left in large quantities in the fields. Fermentation using starter cultures isolated from the fruit (plant-matrix adapted) can have advantages over allochthonous strains in obtaining fermented products with sensory acceptability and potentially probiotic characteristics. This paper details the characterisation of the unripe green tomato lactic microbiota to screen LAB strains for use as starter cultures in fermentation processes, along with LAB strains available from INIAV’s collection. Morphological, biochemical (API system), and genomic (16S rDNA gene sequencing) identification showed that the dominant LAB genera in unripe green tomato are Lactiplantibacillus, Leuconostoc, and Weissella. Among nine tested strains, autochthonous Lactiplantibacillus plantarum and allochthonous Weissella paramesenteroides showed tolerance to added solanine (200 ppm) and the best in vitro probiotic potential. The results indicate that the two LAB strains are promising candidates for manufacturing probiotic fermented foods from unripe green tomatoes.

scholarly journals Isolation and Characterization of Biosurfactant-Producing Bacteria from Amapaense Amazon Soils

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Elisa Maria de Oliveira ◽  
Victor Hugo Gomes Sales ◽  
Marcelo Silva Andrade ◽  
Jerri Édson Zilli ◽  
Wardsson Lustrino Borges ◽  
...  
Keyword(s):  
Surface Tension ◽  
Gene Sequencing ◽  
Biotechnological Applications ◽  
16S Rdna Gene ◽  
Upland Forest ◽  
Isolation And Characterization ◽  
Tension Tests ◽  
Emulsification Capacity ◽  
16S Rdna Gene Sequencing

The objective of this research was to perform screening of biosurfactant-producing bacteria from Amapaense Amazon soils. Floodplain- and upland-forest soils of three municipalities of the Amapá state were isolated and identified. The isolates were cultured in nutrient broth with olive oil, and their extracts were evaluated according to drop collapse, oil dispersion, emulsification, and surface tension tests. From three hundred and eighteen isolates, the 43 bacteria were selected and identified by 16S rDNA gene sequencing, indicating the presence of three different genera, Serratia, Paenibacillus, and Citrobacter. The extracellular biosurfactant production pointed out the 15 most efficient bacteria that presented high emulsification capacity (E24 > 48%) and stability (less than 10% of drop after 72 h) and great potential to reduce the surface tension (varying from 49.40 to 34.50 mN·m−1). Cluster analysis classified genetically related isolates in different groups, which can be connected to differences in the amount or the sort of biosurfactants. Isolates from Serratia genus presented better emulsification capacity and produced a more significant surface tension drop, indicating a promising potential for biotechnological applications.

scholarly journals Rhizospheric rhizobia identification in maize (Zea mays L.) plants

2019 ◽  
Vol 37 (3) ◽  
pp. 255-262
Author(s):  
Reneé Pérez-Pérez ◽  
Maxime Oudot ◽  
Lizette Serrano ◽  
Ionel Hernández ◽  
María Nápoles ◽  
...  
Keyword(s):  
Cell Shape ◽  
Zea Mays L ◽  
Gene Sequencing ◽  
Rhizospheric Soil ◽  
Acid Base ◽  
16S Rdna Gene ◽  
Leguminous Plants ◽  
Maize Cultivars ◽  
Yeast Mannitol Agar ◽  
16S Rdna Gene Sequencing

Rhizobia have been studied for the symbiosis that they establish with the roots of legumes. However, the colonization and promotion of growth in non-leguminous plants has also been demonstrated. The aim of this work was the biochemical and molecular identification of rhizosphere rhizobia present inthe rhizosphere of two commercial maize cultivars. Cultivableisolates were obtained in yeast-mannitol-agar (YMA) mediumfrom rhizospheric soil and the rhizoplane. The cultural (size,color, mucus, etc.), morphological, and staining (cell shape,response to staining and sporulation) characteristics weredetermined as well as isolate responses to eight biochemicaltests (acid-base production, citrate, oxidase, catalase, H2Sproduction, urease, gelatinase and the oxidative-fermentativeassay) that are valuable for rhizobia identification. The genuswas determined by 16S rDNA gene sequencing. We obtained 81total isolates of which 30.86% showed the cultural, morphological and staining characteristics expected for rhizobia and only 20% of these corresponded to the genus Rhizobium.

scholarly journals Effect of 3-Phenyllactic Acid and 3-Phenyllactic Acid-Producing Lactic Acid Bacteria on the Characteristics of Alfalfa Silage

Agriculture ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 10 ◽  
Author(s):  
Zhe Wu ◽  
Shengyang Xu ◽  
Ying Yun ◽  
Tingting Jia ◽  
Zhu Yu
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Gene Sequencing ◽  
16S Rdna Gene ◽  
Phenyllactic Acid ◽  
Colony Forming Units ◽  
Fermentation Quality ◽  
Alfalfa Silage ◽  
16S Rdna Gene Sequencing

In this study, an experiment was performed to evaluate the effect of lactic acid bacteria and 3-phenyllactic acid (PLA) on the fermentation quality and chemical composition of alfalfa silage. Several PLA-tolerant strains were screened from silages and identified. The selected strains (1 × 106 colony forming units/g fresh alfalfa) and PLA (1.0, 2.0, or 3.0 g/kg) were applied to alfalfa before ensiling. After 45 days of storage, the silages were unsealed and subjected to component analysis. Biochemical methods and 16S rDNA gene sequencing were used for the identification of the two strains as Lactobacillus plantarum. The characteristics of chemical and fermentation compounds indicated that PLA and the two strains efficiently improved the quality of the alfalfa silage. It can be concluded that the use of the strains and PLA can significantly improve the quality of silage.

scholarly journals Intraoperative diagnosis of mitral valve endocarditis secondary to Paenibacillus provencensis

2017 ◽  
Vol 99 (2) ◽  
pp. e54-e55 ◽  
Author(s):  
A-C Pinho-Gomes ◽  
A Nasir ◽  
R Mosca ◽  
S Mirza ◽  
I Kadir
Keyword(s):  
Infective Endocarditis ◽  
Mitral Valve ◽  
Aortic Valve ◽  
Gene Sequencing ◽  
Transoesophageal Echocardiography ◽  
16S Rdna Gene ◽  
Intraoperative Diagnosis ◽  
First Case ◽  
Mitral Valve Vegetation ◽  
16S Rdna Gene Sequencing

We report the first case of infective endocarditis caused by Paenibacillus provencensis. A mitral valve vegetation was incidentally discovered by intraoperative transoesophageal echocardiography in a 70-year-old woman undergoing aortic valve replacement. The precise identification of the causative agent was by means of genotypic characterisation with 16S rDNA gene sequencing. The patient was successfully treated with a 6-week course of antibiotics postoperatively, following debridement of the valve vegetation.

scholarly journals The effect of sodium carboxymethyl starch with high degree of substitution on defecation

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257012
Author(s):  
Wu-dang Lu ◽  
Man-li Wu ◽  
Jun-xia Zhang ◽  
Ting-ting Huang ◽  
Shuai-shuai Du ◽  
...  
Keyword(s):  
Fatty Acids ◽  
16S Rdna ◽  
Gene Sequencing ◽  
Short Chain Fatty Acids ◽  
16S Rdna Gene ◽  
Carboxymethyl Starch ◽  
Rdna Gene ◽  
High Degree ◽  
Sodium Carboxymethyl Starch ◽  
16S Rdna Gene Sequencing

Sodium carboxymethyl starch (CMS-Na), a kind of food additive with high degree of substitution, is also known as a prebiotic. The aim of this study was to determine the effect of CMS-Na on defecation. Constipated mouse model was prepared by loperamide. Normal rats were also used in the study. Short-chain fatty acids in rat feces were detected by gas chromatography. The bacterial communities in rat feces were identified by 16S rDNA gene sequencing. 5-hydroxytryptamine (5-HT) and tryptophan hydroxylase 1 (Tph1) were measured by ELISA. The results showed that CMS-Na increased the fecal granule counts and intestinal propulsion rate in constipated mice. The contents of water, acetic acid, propionic acid and n-butyrate in feces, Tph1 in colon and 5-HT in serum of rats were increased. In addition, CMS-Na shortened the colonic transport time in rats. The 16S rDNA gene sequencing results indicated that CMS-Na increased the relative abundance of Alloprevotella and decreased the proportion of Lactobacillus. However, the biodiversity of the normal intestinal flora was not altered. In conclusion, CMS-Na can promote defecation in constipated mice. The mechanism may be related to the regulation of Alloprevotella and Lactobacillus in colon, the increase of short-chain fatty acids, and the promotion of the synthesis of Tph1 and 5-HT.

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