The expansion of porous glass on the adsorption of non-polar gases

The design and construction of an absolute dilatometer of high sensitivity to measure the small expansions resulting from the adsorption of non-polar gases on to porous glass at liquid-air temperatures are described. The expansions measured with this instrument are the first recorded with a rigid adsorbent for the case in which the adsorption is, with certainty, physical in nature. Experiments in which the gases argon, nitrogen, oxygen, hydrogen and krypton were used as adsorbates are reported. The effect of organic matter adsorbed on to the glass from the atmosphere, of unknown composition, on the expansion characteristics is indicated. A technique for the removal of this organic matter in situ without affecting the area of the glass is described. The results have confirmed the correctness of an equation which has been derived by the author (Yates 1952) (∂ F /∂ V ) T = –3/2 K , where ∂ V is the volumetric expansion at constant temperature resulting from ∂ F , the surface free energy lowering, and where K is the bulk modulus of the adsorbent.

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TO SEPARATELY ASSESS THE DIAGNOSTIC POTENTIAL OF TVS & MRI IN THE TERMS OF SENSITIVITY AND SPECIFICITY BY CORRELATING THE IMAGING FINDINGS WITH HISTOPATHOLOGY

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v4i6.1209 ◽

2020 ◽

Vol 4 (6) ◽

Author(s):

Suraj Mathur

Keyword(s):

Transvaginal Ultrasound ◽

High Sensitivity ◽

Imaging Evaluation ◽

Medical College ◽

Conventional Mri ◽

Diagnostic Potential ◽

Adc Mapping ◽

Malignant Lesions ◽

Sensitivity Specificity

This prospective study was done in the Department of Radio diagnosis Govt. Medical College, Kozhikode. A total of 65 patients who were referred to our department with clinical suspicion of endometrial lesions and incidentally detected endometrial lesions on ultrasonography underwent transvaginal ultrasound and subsequent Imaging evaluation of pelvis MRI has very high sensitivity (95%) and specificity (98%) and is almost as accurate (97%) as histopathology in differentiating benign from malignant lesions. Addition of DWI with ADC mapping to conventional MRI increases its accuracy even more. However there is inherent limitation to MRI in detecting carcinoma in situ and micrometastasis. Keywords: TVS, MRI, Sensitivity, Specificity, Histopathology.

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In Situ High Sensitivity Brillouin Light Scattering Spectrometer for MBE-Grown Thin Films

10.21236/ada388738 ◽

2001 ◽

Author(s):

Charles M. Falco ◽

David J. Keavney

Keyword(s):

Thin Films ◽

Light Scattering ◽

High Sensitivity ◽

Brillouin Light Scattering

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In situ organic matter degradability of untreated and urea-treated varieties of spring barley and oat straws, and of untreated varieties of winter wheat straws

Animal Feed Science and Technology ◽

10.1016/0377-8401(92)90121-l ◽

1992 ◽

Vol 37 (1-2) ◽

pp. 73-84 ◽

Cited By ~ 7

Author(s):

P.E. Colucci ◽

D. Falk ◽

G.K. Macleod ◽

D.G. Grieve

Keyword(s):

Organic Matter ◽

Winter Wheat ◽

Spring Barley

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Spatial expression pattern of serine proteases in the blood fluke Schistosoma mansoni determined by fluorescence RNA in situ hybridization

Parasites & Vectors ◽

10.1186/s13071-021-04773-8 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Lenka Ulrychová ◽

Pavel Ostašov ◽

Marta Chanová ◽

Michael Mareš ◽

Martin Horn ◽

...

Keyword(s):

In Situ Hybridization ◽

Schistosoma Mansoni ◽

Rna Sequencing ◽

Serine Proteases ◽

Expression Patterns ◽

High Sensitivity ◽

Host Parasite Interactions ◽

Highly Sensitive ◽

Host Parasite

Abstract Background The blood flukes of genus Schistosoma are the causative agent of schistosomiasis, a parasitic disease that infects more than 200 million people worldwide. Proteases of schistosomes are involved in critical steps of host–parasite interactions and are promising therapeutic targets. We recently identified and characterized a group of S1 family Schistosoma mansoni serine proteases, including SmSP1 to SmSP5. Expression levels of some SmSPs in S. mansoni are low, and by standard genome sequencing technologies they are marginally detectable at the method threshold levels. Here, we report their spatial gene expression patterns in adult S. mansoni by the high-sensitivity localization assay. Methodology Highly sensitive fluorescence in situ RNA hybridization (FISH) was modified and used for the localization of mRNAs encoding individual SmSP proteases (including low-expressed SmSPs) in tissues of adult worms. High sensitivity was obtained due to specifically prepared tissue and probes in combination with the employment of a signal amplification approach. The assay method was validated by detecting the expression patterns of a set of relevant reference genes including SmCB1, SmPOP, SmTSP-2, and Sm29 with localization formerly determined by other techniques. Results FISH analysis revealed interesting expression patterns of SmSPs distributed in multiple tissues of S. mansoni adults. The expression patterns of individual SmSPs were distinct but in part overlapping and were consistent with existing transcriptome sequencing data. The exception were genes with significantly low expression, which were also localized in tissues where they had not previously been detected by RNA sequencing methods. In general, SmSPs were found in various tissues including reproductive organs, parenchymal cells, esophagus, and the tegumental surface. Conclusions The FISH-based assay provided spatial information about the expression of five SmSPs in adult S. mansoni females and males. This highly sensitive method allowed visualization of low-abundantly expressed genes that are below the detection limits of standard in situ hybridization or by RNA sequencing. Thus, this technical approach turned out to be suitable for sensitive localization studies and may also be applicable for other trematodes. The results suggest that SmSPs may play roles in diverse processes of the parasite. Certain SmSPs expressed at the surface may be involved in host–parasite interactions. Graphic abstract

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High Sensitivity of PCR in Situ Hybridization for the Detection of Human Papillomavirus Infection in Uterine Cervical Neoplasias

Gynecologic Oncology ◽

10.1006/gyno.2001.6261 ◽

2001 ◽

Vol 82 (2) ◽

pp. 350-354 ◽

Cited By ~ 5

Author(s):

Yuhong Xiao ◽

Shigemi Sato ◽

Takaaki Oguchi ◽

Kaori Kudo ◽

Yoshihito Yokoyama ◽

...

Keyword(s):

In Situ Hybridization ◽

Human Papillomavirus ◽

High Sensitivity ◽

Human Papillomavirus Infection ◽

Papillomavirus Infection ◽

Pcr In Situ

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New insight into quinones triggered ferrate in-situ synthesized polynuclear Fe-hydroxyl complex for enhancing interfacial adsorption in highly efficient removal of natural organic matter

The Science of The Total Environment ◽

10.1016/j.scitotenv.2020.144844 ◽

2021 ◽

Vol 770 ◽

pp. 144844

Author(s):

Guang Yang ◽

Jie Wang ◽

Hongwei Zhang ◽

Hui Jia ◽

Yang Zhang ◽

...

Keyword(s):

Organic Matter ◽

Natural Organic Matter ◽

Efficient Removal ◽

Highly Efficient ◽

Interfacial Adsorption ◽

Insight Into

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High-Sensitivity In situ Fluorescence Imaging of Ytterbium Atoms in a Two-Dimensional Optical Lattice with Dual Optical Molasses

Journal of the Physical Society of Japan ◽

10.7566/jpsj.83.014301 ◽

2014 ◽

Vol 83 (1) ◽

pp. 014301 ◽

Cited By ~ 4

Author(s):

Kosuke Shibata ◽

Ryuta Yamamoto ◽

Yoshiro Takahashi

Keyword(s):

Fluorescence Imaging ◽

Optical Lattice ◽

High Sensitivity ◽

Two Dimensional ◽

Optical Molasses

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Lipid biomarkers in Holocene and glacial sediments from ancient Lake Ohrid (Macedonia, Albania)

Biogeosciences ◽

10.5194/bg-7-3473-2010 ◽

2010 ◽

Vol 7 (11) ◽

pp. 3473-3489 ◽

Cited By ~ 39

Author(s):

J. Holtvoeth ◽

H. Vogel ◽

B. Wagner ◽

G. A. Wolff

Keyword(s):

Organic Matter ◽

Lipid Biomarkers ◽

Lake Ohrid ◽

Labile Organic Matter ◽

Glacial Sediments ◽

Plant Matter ◽

Lipid Biomarker ◽

First Results ◽

Near Shore

Abstract. Organic matter preserved in Lake Ohrid sediments originates from aquatic and terrestrial sources. Its variable composition reflects climate-controlled changes in the lake basin's hydrology and related organic matter export, i.e. changes in primary productivity, terrestrial plant matter input and soil erosion. Here, we present first results from lipid biomarker investigations of Lake Ohrid sediments from two near-shore settings: site Lz1120 near the southern shore, with low-lying lands nearby and probably influenced by river discharge, and site Co1202 which is close to the steep eastern slopes. Variable proportions of terrestrial n-alkanoic acids and n-alkanols as well as compositional changes of ω-hydroxy acids document differences in soil organic matter supply between the sites and during different climate stages (glacial, Holocene, 8.2 ka cooling event). Changes in the vegetation cover are suggested by changes in the dominant chain length of terrestrial n-alkanols. Effective microbial degradation of labile organic matter and in situ contribution of organic matter derived from the microbes themselves are both evident in the sediments. We found evidence for anoxic conditions within the photic zone by detecting epicholestanol and tetrahymanol from sulphur-oxidising phototrophic bacteria and bacterivorous ciliates and for the influence of a settled human community from the occurrence of coprostanol, a biomarker for human and animal faeces (pigs, sheep, goats), in an early Holocene sample. This study illustrates the potential of lipid biomarkers for future environmental reconstructions using one of Europe's oldest continental climate archives, Lake Ohrid.

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