Control of ripening

Ripening of fleshy fruits involves major changes in physiology and biochemistry that alter their colour, flavour, texture, aroma and nutritional value. These changes affect all cell compartments and require the expression of new genes encoding enzymes that catalyse reactions essential for the development of quality attributes. In climacteric fruits, such as tomato, ethylene functions as a hormone to stimulate changes in gene expression required for ripening. Molecular cloning experiments have led to the isolation of cDNAs encoding many ripening proteins. This has enabled the identification and manipulation of novel plant genes encoding enzymes involved in cell wall texture change, carotenoid biosynthesis, ethylene synthesis and the identification of gene control regions involved in fruit-specific, ripening-specific, and ethylene-regulated gene expression. Antisense and partial sense gene techniques have been developed to generate genetically modified plant lines in which specific genes have been permanently inactivated. These fundamental studies have led to production and evaluation of genetically modified tomato lines with improved colour, texture, storage life, and processing characteristics. Zeneca Seeds has established a new business division, the aim of which is to utilize these techniques for the development of improved fruit and vegetable varieties. In collaboration with Petoseed, Zeneca Seeds is in the process of transferring the genes leading to quality im provement of tomatoes to Petoseed’s elite tomato germplasm. The primary focus is on the development of improved processing hybrids. These are being evaluated in collaboration with Hunt Wesson, a large and diversified tomato processing company. It is planned that products based on this research will be introduced in the USA in 1995.