scholarly journals Sensitivity to Bacillus subtilis: A Novel System for Selection of Heterozygous Diploids of Dictyostelium discoideum

1977 ◽  
Vol 100 (1) ◽  
pp. 207-211 ◽  
Author(s):  
P. C. NEWELL ◽  
R. F. HENDERSON ◽  
D. MOSSES ◽  
D. I. RATNER
Keyword(s):  
Bacillus Subtilis ◽  
Dictyostelium Discoideum ◽  
Selection Of

The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Hussein A Kadhum ◽  
Thualfakar H Hasan2
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Bacterial Growth ◽  
Broad Spectrum ◽  
Inhibitory Activity ◽  
Bacterial Pathogens ◽  
Inhibitory Ability ◽  
Selection Of

The study involved the selection of two isolates from Bacillus subtilis to investigate their inhibitory activity against some bacterial pathogens. B sub-bacteria were found to have a broad spectrum against test bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. They were about 23-30 mm and less against Klebsiella sp. The sensitivity of some antibodies was tested on the test samples. The results showed that the inhibitory ability of bacterial growth in the test samples using B. subtilis extract was more effective than the antibiotics used.

NEW LOCI IN DICTYOSTELIUM DISCOIDEUM DETERMINING PIGMENT FORMATION AND GROWTH ON BACILLUS SUBTILIS

Genetics ◽  
1980 ◽  
Vol 96 (1) ◽  
pp. 115-123
Author(s):  
James H Morrissey ◽  
Steven Wheeler ◽  
William F Loomis
Keyword(s):  
Bacillus Subtilis ◽  
Dictyostelium Discoideum ◽  
Type Strain ◽  
Wild Type Strain ◽  
Wild Type ◽  
Linkage Groups ◽  
New Mutation ◽  
Pigment Formation ◽  
Complementation Groups ◽  
First Time

ABSTRACT Seventeen independently isolated pigmentless (white) mutations in Dictyostelium discoideum are all recessive and fall into three complementation groups identifying two new whi loci in addition to the previously characterized whiA locus. whiB and whiC map to linkage groups III and IV, respectively. In addition, it was discovered that our laboratory stock of NC4, the wild-type strain from which these mutants were derived, has spontaneously lost the ability to grow on Bacillus subtilis. This new mutation, bsgB500, maps to linkage group VII and is not allelic to bsgA. bsgB500 is the first spontaneously derived mutation in D. discoideum that can be used to select heterozygous diploids, and for the first time allows genetic analysis to be routinely performed on strains derived from an unmutagenized background.

scholarly journals SELECTION OF MEDIUM FOR BIOPESTICIDES FERMENTATION PROCESS BY BACILLUS SUBTILIS AAF2 UAAC 20701

2018 ◽  
Vol 9 (8) ◽  
pp. 21-26 ◽  
Author(s):  
Akmal Djamaan ◽  
Anthoni Agustien ◽  
Syukria Ikhsan Zam ◽  
Miftahul Jannah ◽  
Rika Sari Lalfari ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Fermentation Process ◽  
Selection Of

Selection of effective and highly thermostable Bacillus subtilis lipase A template as an industrial biocatalyst-A modern computational approach

2015 ◽  
Vol 10 (6) ◽  
pp. 508-519 ◽  
Author(s):  
B. Senthilkumar ◽  
D. Meshachpaul ◽  
Rao Sethumadhavan ◽  
R. Rajasekaran
Keyword(s):  
Bacillus Subtilis ◽  
Computational Approach ◽  
Lipase A ◽  
Selection Of

scholarly journals The temperature of growth and sporulation modulates the efficiency of spore-display in Bacillus subtilis

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Claudia Petrillo ◽  
Stefany Castaldi ◽  
Mariamichela Lanzilli ◽  
Anella Saggese ◽  
Giuliana Donadio ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Spore Production ◽  
Bacterial Spores ◽  
Heterologous Proteins ◽  
Dna Coding ◽  
Gene Coding ◽  
Different Temperatures ◽  
Mucosal Vaccines ◽  
Heterologous Antigens ◽  
Selection Of

Abstract Background Bacterial spores displaying heterologous antigens or enzymes have long been proposed as mucosal vaccines, functionalized probiotics or biocatalysts. Two main strategies have been developed to display heterologous molecules on the surface of Bacillus subtilis spores: (i) a recombinant approach, based on the construction of a gene fusion between a gene coding for a coat protein (carrier) and DNA coding for the protein to be displayed, and (ii) a non-recombinant approach, based on the spontaneous and stable adsorption of heterologous molecules on the spore surface. Both systems have advantages and drawbacks and the selection of one or the other depends on the protein to be displayed and on the final use of the activated spore. It has been recently shown that B. subtilis builds structurally and functionally different spores when grown at different temperatures; based on this finding B. subtilis spores prepared at 25, 37 or 42 °C were compared for their efficiency in displaying various model proteins by either the recombinant or the non-recombinant approach. Results Immune- and fluorescence-based assays were used to analyze the display of several model proteins on spores prepared at 25, 37 or 42 °C. Recombinant spores displayed different amounts of the same fusion protein in response to the temperature of spore production. In spores simultaneously displaying two fusion proteins, each of them was differentially displayed at the various temperatures. The display by the non-recombinant approach was only modestly affected by the temperature of spore production, with spores prepared at 37 or 42 °C slightly more efficient than 25 °C spores in adsorbing at least some of the model proteins tested. Conclusion Our results indicate that the temperature of spore production allows control of the display of heterologous proteins on spores and, therefore, that the spore-display strategy can be optimized for the specific final use of the activated spores by selecting the display approach, the carrier protein and the temperature of spore production.

scholarly journals In Vitro Mutagenesis of Bacillus subtilis by Using a Modified Tn7 Transposon with an Outward-Facing Inducible Promoter

2008 ◽  
Vol 74 (11) ◽  
pp. 3419-3425 ◽  
Author(s):  
Christophe Bordi ◽  
Bronwyn G. Butcher ◽  
Qiaojuan Shi ◽  
Anna-Barbara Hachmann ◽  
Joseph E. Peters ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
High Frequency ◽  
Inducible Promoter ◽  
In Vitro Mutagenesis ◽  
Donor Plasmid ◽  
Positive Bacterium ◽  
Expression Of Genes ◽  
Regulated Expression ◽  
Selection Of

ABSTRACT A Tn7 donor plasmid, pTn7SX, was constructed for use with the model gram-positive bacterium Bacillus subtilis. This new mini-Tn7, mTn7SX, contains a spectinomycin resistance cassette and an outward-facing, xylose-inducible promoter, thereby allowing for the regulated expression of genes downstream of the transposon. We demonstrate that mTn7SX inserts are obtained at a high frequency and occur randomly throughout the B. subtilis genome. The utility of this system was demonstrated by the selection of mutants with increased resistance to the antibiotic fosfomycin or duramycin.

scholarly journals Selection of the main micronutrients for the production of biosurfactant by an isolated Bacillus subtilis strain

2019 ◽  
Vol 9 (4) ◽  
Author(s):  
Mariana Ruiz Frazão Santiago ◽  
Vitor da Silva Liduino ◽  
Lizeth Jaramillo ◽  
Paula Fernandes de Aguiar
Keyword(s):  
Bacillus Subtilis ◽  
Subtilis Strain ◽  
Selection Of

scholarly journals Evolving social behaviour through selection of single-cell adhesion in Dictyostelium discoideum

2021 ◽  
Author(s):  
Sandrine Adiba ◽  
Mathieu Forget ◽  
Silvia De Monte
Keyword(s):  
Cell Adhesion ◽  
Dictyostelium Discoideum ◽  
Experimental Evolution ◽  
Social Behaviour ◽  
Social Performance ◽  
Fruiting Bodies ◽  
Social Success ◽  
The Social ◽  
Different Strains ◽  
Selection Of

The social amoeba Dictyostelium discoideum commonly forms chimeric fruiting bodies by aggregation of different strains. Genetic variants that produce a higher proportion of spores are predicted to undercut multicellular organization unless cooperators assort positively. Cell adhesion is considered a primary factor driving such assortment, but evolution of adhesion has not been experimentally connected to changes in social performance. In this study we modified by experimental evolution the properties of individual cells, selecting for higher and lower adhesion to substrate. We then quantified the effects of these changes on cell-cell adhesion, development, and social behaviour. Unlike strains selected based on relative reproductive success in the social stage, we found that in binary chimeras both derived strains produce a smaller fraction of spores than the ancestor. Thus, evolution appears to have produced social cooperators. Examination of development revealed that this is however achieved via two opposed paths. Cells selected to be more adhesive to the substrate disproportionately contribute to the structural stability of fruiting bodies, as one would expect for cooperators. On the contrary, less adhesive cells behave as cheaters that undermine their own success more than that of the ancestor. These differences are reflected by a metric for social success that generalizes the classically used variation in frequency during the multicellular phase. Our work shows that cell mechanical interactions can constrain evolution of development and assortment in chimeras, and calls for integrating cell-level processes in conceptualizing the emergence of multicellular organization.

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