scholarly journals Planctomonas psychrotolerans sp. nov., isolated from rhizosphere soil of Suaeda salsa

2020 ◽  
Vol 70 (10) ◽  
pp. 5271-5279 ◽  
Author(s):  
Xiao-Jun Li ◽  
Chun-Miao Wang ◽  
Xue-Mei Feng ◽  
Shao-Wei Liu ◽  
Hai-Xia Qiao ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Suaeda Salsa ◽  
Bootstrap Support ◽  
Alkaline Soil ◽  
Content Type ◽  
Diaminobutyric Acid ◽  
Link Type ◽  
Pr China ◽  
High Bootstrap

A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China. Cells of the isolate were Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci. Strain J5903T grew optimally at 20‒25 °C, at pH 7.0‒7.5 and with <1 % (w/v) NaCl. The cell-wall peptidoglycan type was B2γ with d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid as diagnostic amino acids. The muramyl residue was acetyl type. The menaquinones were MK-11, MK-12, MK-10 and MK-13. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid. The major whole-cell fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. The genomic DNA G+C content was 69.1 mol%. It shared the highest average nucleotide identity and digital DNA–DNA hybridization values with Planctomonas deserti 13S1-3T. Phylogenies based on genome sequence showed that strain J5903T and P. deserti 13S1-3T formed a robust cluster with high bootstrap support. Strain J5903T shared typical chemotaxonomic characteristics with P. deserti 13S1-3T. Combining the polyphasic taxonomic evidence, strain J5903T represents a novel species of the genus Planctomonas , for which the name Planctomonas psychrotolerans sp. nov. is proposed. The type strain is J5903T (=DSM 101894T=CGMCC 1.15523T).

Pseudoroseicyclus tamaricis sp. nov., isolated from seashore sediment of a Tamarix chinensis forest and emended descriptions of the genus Pseudoroseicyclus Park et al. 2016

2021 ◽  
Vol 71 (7) ◽  
Author(s):  
Yingbao Gai ◽  
Yongpeng Yang ◽  
Xiupian Liu ◽  
Guangyu Li ◽  
Shanshan Wang ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Marine Ecology ◽  
Rrna Gene ◽  
Tamarix Chinensis ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Pr China ◽  
Gene Similarity

An aerobic, Gram-stain-negative, bacterium, designated CLL3-39T was isolated from seashore sediment collected at a Tamarix chinensis forest in the Marine Ecology Special Reserve of Changyi, Shandong Province, PR China. Cells of strain CLL3-39T were olive-shaped and no flagellum was observed. Strain CLL3-39T grew optimally at 33 °C, pH 7.5 and salinity (sea salts) of 40 g l−1. The main fatty acids in the cell membrane of strain CLL3-39T comprised anteiso-C15 : 0 (22.3 %), iso-C15 : 0 (14.0 %), C16 : 0 (9.2 %) and summed feature 8 (contains C18 : 1  ω7c/C18 : 1  ω6c. 26. 7 %). The main polar lipids of CLL3-39T were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. The respiratory quinone was Q10. The G+C content of the genomic DNA of strain CLL3-39T was 69.6 mol%. The average nucleotide identity between CLL3-39T and Pseudoroseicyclus aestuarii DB-4T was 74.7 % and the in silico DNA–DNA hybridization value was 20.1 %. Phylogenetically, strain CLL3-39T belonged to the genus Pseudoroseicyclus , branching with only one type strain P. aestuarii DB-4T with 96.3 % 16S rRNA gene similarity, followed by Limimaricola cinnabarinus LL-001T (95.2 %). Based on its phenotypic, phylogenetic and chemotaxonomic characteristics, we propose strain CLL3-39T (=MCCC 1A14815T =KCTC 72665T) as a representative of a novel species in the genus Pseudoroseicyclus , for which the name Pseudoroseicyclus tamaricis sp. nov. is proposed.

scholarly journals Lysinibacillus halotolerans sp. nov., isolated from saline-alkaline soil

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2593-2598 ◽  
Author(s):  
Delong Kong ◽  
Yanwei Wang ◽  
Bingqiang Zhao ◽  
Yanting Li ◽  
Jinlong Song ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Novel Species ◽  
Soil Samples ◽  
Rrna Gene ◽  
Alkaline Soil ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
Unknown Lipid

A novel aerobic, halotolerant bacterium, designated strain LAM612T, was isolated from saline-alkaline soil samples from Lingxian County, Shandong Province, China. Cells of strain LAM612T were Gram-reaction-positive, endospore-forming, motile and rod-shaped. The optimal temperature and pH for growth were 35 °C and pH 6.0, respectively. Strain LAM612T could grow in the presence of up to 10 % (w/v) NaCl. The genomic DNA G+C conten was 36.4 mol% as detected by the T m method. Comparative analysis of 16S rRNA gene sequences revealed that LAM612T was closely related to Lysinibacillus sinduriensis KACC 16611T (98.0 %), L. chungkukjangi KACC 16626T (97.5 %), L. massiliensis KCTC 13178T (97.4 %), L. xylanilyticus KACC 15113T (97.2 %), L. macroides DSM 54T (97.0 %) and L. manganicus DSM 26584T (96.5 %). The DNA–DNA hybridization values between strain LAM612T and its closest relatives ranged from 20.6 % to 41.9 %. The major fatty acids of strain LAM612T were iso-C15 : 0 (40.8 %), iso-C16 : 0 (15.2 %) and anteiso-C15 : 0 (10.8 %). The cell-wall peptidoglycan content was A4α (l-Lys–d-Asp). The predominant menaquinone was MK-7 and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unknown phospholipids, five unknown glycolipids and an unknown lipid. Based on the DNA–DNA hybridization results and phenotypic, phylogenetic and chemotaxonomic properties, strain LAM612T could be distinguished from the recognized species of the genus Lysinibacillus , and was suggested to represent a novel species of this genus, for which the name Lysinibacillus halotolerans sp. nov. is proposed. The type strain is LAM612T ( = ACCC 00718T = JCM 19611T).

scholarly journals Pseudoxanthobacter liyangensis sp. nov., isolated from dichlorodiphenyltrichloroethane-contaminated soil

2014 ◽  
Vol 64 (Pt_10) ◽  
pp. 3390-3394 ◽  
Author(s):  
Xiao-Mei Liu ◽  
Kai Chen ◽  
Chao Meng ◽  
Long Zhang ◽  
Jian-Chun Zhu ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Dna Hybridization ◽  
Type Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Hybridization Data ◽  
Pr China

An aerobic, Gram-stain negative, pale, rod-shaped, nitrogen-fixing bacterial strain, DDT-3T, was isolated from dichlorodiphenyltrichloroethane-contaminated soil in Liyang, PR China. Strain DDT-3T grew at temperatures ranging from 20 to 40 °C (optimum 30–37 °C) and a pH of between 5.0 and 10.0 (optimum pH 7.0–8.0). The G+C content of the total DNA was 70.1 mol%. The 16S rRNA gene sequence of strain DDT-3T showed the highest similarity to that of Pseudoxanthobacter soli CC4T (99.6 %), followed by Kaistia dalseonensis B6-8T (93.3 %), Kaistia soli 5YN9-8T (93.0 %) and Amorphus orientalis YIM D10T (93.0 %). Strain DDT-3T showed less than 92.6 % similarity with other species of the family Xanthobacteraceae . The major cellular fatty acids of strain DDT-3T were C19 : 0 cyclo ω8c (42.6 %), C16 : 0 (33.2 %) and C18 : 1ω7c (10.0 %). The only respiratory quinone was ubiquinone Q-10. The characteristic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The major polar lipids were glycolipid, lipid, phosphatidylcholine, aminolipid, phosphatidylmethylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The polyamine profile consisted of major amounts of putrescine (92.9 %) and minor amounts of spermidine (5.0 %) and spermine (2.1 %). These chemotaxonomic data support the affiliation of strain DDT-3T with the genus Pseudoxanthobacter . The DNA–DNA hybridization value between strain DDT-3T and strain CC4T was 47.8 % (reciprocal 44.3 %). DNA–DNA hybridization data as well as the biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiation of strain DDT-3T and strain CC4T. Strain DDT-3T, therefore, represents a novel species of the genus Pseudoxanthobacter , for which the name Pseudoxanthobacter liyangensis sp. nov. is proposed. The type strain is DDT-3T ( = KACC 16601T = CCTCC AB 2013167T).

scholarly journals Phytoactinopolyspora mesophila sp. nov., isolated from a saline–alkaline soil

2020 ◽  
Vol 70 (7) ◽  
pp. 4179-4185 ◽  
Author(s):  
Xue-Mei Feng ◽  
Xue-Han Zhai ◽  
Shao-Wei Liu ◽  
Chun-Miao Wang ◽  
Cheng-Hang Sun ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Phylogenetic Trees ◽  
Rrna Gene ◽  
Alkaline Soil ◽  
Content Type ◽  
Link Type ◽  
Pr China ◽  
Inner Mongolia Autonomous Region

A novel moderately halophilic, filamentous actinobacterium, designated as XMNu-373T, was isolated from a saline–alkaline soil sample collected from the Mongolia Plateau, Dongwu County, Inner Mongolia Autonomous Region, PR China. The isolate grew optimally at 28‒37 °C, pH 7.0‒8.0 and with 2–5 % (w/v) NaCl. The substrate mycelia fragmented into rod-like elements, and the white aerial mycelia formed spore chains at maturity. The predominant menaquinone was MK-9(H4). The polar lipids were diphosphatidylglycerol, three unidentified phosphoglycolipids, an unidentified aminophospholipid, two phosphatidylinositol mannosides, four unidentified phospholipids, phosphatidylglycerol and two unidentified lipids. The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0. The genomic DNA G+C content was 66.2 mol%. It shared high 16S rRNA gene sequence similarities to Phytoactinopolyspora halotolerans YIM 96448T (96.1 %) and Phytoactinopolyspora endophytica EGI 60009T (96.0 %). Phylogenetic trees based on 16S rRNA gene sequences revealed that strain XMNu-373T resided in the clade of family Jiangellaceae , and it formed a monophyletic branch distinct from four other recognized type species in the subclade of the genus Phytoactinopolyspora . On the basis of polyphasic taxonomic evidence, strain XMNu-373T represents a novel species of the genus Phytoactinopolyspora , for which the name Phytoactinopolyspora mesophila sp. nov. is proposed. The type strain is XMNu-373T (=JCM 33740T=CGMCC 4.7654T).

Actinomadura litoris sp. nov., an actinobacterium isolated from sandy soil in Sanya

2021 ◽  
Author(s):  
Peng Cao ◽  
Xi Xu ◽  
Chenxu Li ◽  
Liyuan Han ◽  
Wenhao Mu ◽  
...  
Keyword(s):  
Sandy Soil ◽  
Dna Hybridization ◽  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Optimum Ph ◽  
Pr China

A novel actinobacterium, designated strain NEAU-AAG5T, was isolated from sandy soil collected from Niuwang island in Sanya, Hainan Province, PR China. The taxonomic position of the strain was investigated using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NEAU-AAG5T belongs to the genus Actinomadura and shared highest sequence similarity with Actinomadura macra NBRC 14102T (98.8 %). Strain NEAU-AAG5T grows at 20–40 °C (optimum, 28 °C), pH 6–10 (optimum, pH 7) and has NaCl tolerance of 0–3 %. The menaquinones were identified as MK-9(H4) (4.2 %), MK-9(H6) (49.2 %) and MK-9(H8) (46.5 %). The major fatty acids were C16 : 0 (31.4 %), 10-methyl C18 : 0 (21.3 %) and C18 : 1  ω9c (15.7 %). The polar lipids were diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside, phosphatidylglycerol and phosphoglycolipid. The genomic DNA G+C content of strain NEAU-AAG5T based on whole genome sequences was 72.8 mol%. Digital DNA–DNA hybridization between strain NEAU-AAG5T and its closest phylogenetic neighbour, A. macra NBRC 14102T, resulted in similarity value of 28.0 % (<70 %). Additionally, the average nucleotide identity was 84.2 % for A. macra NBRC 14102T. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-AAG5T can be characterized to represent a novel species of the genus Actinomadura , for which the name Actinomadura litoris sp. nov. is proposed. The type strain is NEAU-AAG5T (=JCM 33456T=CCTCC AA 2019043T).

Paraliobacillus salinarum sp. nov., isolated from saline soil in Yingkou, China

2021 ◽  
Vol 71 (7) ◽  
Author(s):  
Ya-Lin Yin ◽  
Fang-Ling Li ◽  
Xian-Yuan Du ◽  
Ya-Xi Zhang ◽  
Lei Wang
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Saline Soil ◽  
Phylogenetic Position ◽  
16S Rrna Analysis ◽  
Content Type ◽  
Link Type ◽  
A Value ◽  
Optimum Ph ◽  
Pr China

A novel Gram-stain-positive, facultatively aerobic, slightly halophilic, endospore-forming bacterium, designated G6-18T, was isolated from saline soil collected in Yingkou, Liaoning, PR China. Cells of strain G6-18T grew at 10–37 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 8.0) and in the presence of 2–15 % (w/v) NaCl (optimum, 5 %). The strain could be clearly distinguished from the related species of the genus Paraliobacillus by its phylogenetic position and biochemical characteristics. It presented MK-7 as the major quinone and the dominant cellular fatty acids were iso-C16 : 0, anteiso-C15 : 0, C16 : 0 and iso-C14 : 0. The polar lipids consisted of diphosphatidylglycerol and phosphatidylglycerol as the major components. The G+C content of strain G6-18T genome was 35.3 mol%. 16S rRNA analysis showed that strain G6-18T had the highest similarity to Paraliobacillus ryukyuensis DSM 15140T, reaching 97.0 %, followed by Paraliobacillus quinghaiensis CGMCC 1.6333T with a value of 96.3 %. The average nucleotide identity values between strain G6-18T and Paraliobacillus ryukyuensis DSM 15140T, Paraliobacillus sedimins KCTC 33762T, Paraliobacillus quinghaiensis CGMCC 1.6333T and Paraliobacillus zengyii DSM 107811T were 74.3, 72.0, 73.2 and 72.8 %, respectively, and the digital DNA–DNA hybridization values between strain G6-18T and the neighbouring strains were 15.6, 13.8, 14.2 and 14.2 %, respectively. Based on phenotypic, chemotaxonomic and phylogenetic inferences, strain G6-18T represents a novel species of the genus Paraliobacillus , for which the name Paraliobacillus salinarum sp. nov. (=CGMCC 1.12058T=DSM 25428T) is proposed.

Undibacterium crateris sp. nov., isolated from water of crater lake

2021 ◽  
Vol 71 (4) ◽  
Author(s):  
Dorji Phurbu ◽  
Zi-Xuan Liu ◽  
Hong-Can Liu ◽  
Yangzom Pema ◽  
Lhamo Yungchen ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Crater Lake ◽  
Rrna Gene ◽  
Sequencing Data ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Pr China ◽  
Hinggan Mountain

A Gram-stain-negative, aerobic, flagellated, non-spore-forming, rod-shaped bacterium, named B2R-29T, was isolated from water collected from a crater lake on Da Hinggan mountain, PR China. Strain B2R-29T was oxidase- and catalase-positive. On the basis of the results of 16S rRNA gene sequence analyses, strain B2R-29T clearly belonged to the family Oxalobacteraceae , class Betaproteobacteria and showed the highest similarity to Undibacterium oligocarboniphilum EM1T (97.4 %) and to the other species of Undibacterium (less than 96.8 %). In the phylogenetic tree, strain B2R-29T formed a clade with U. oligocarboniphilum EM1T and Undibacterium squillarum CMJ-9T, indicating that is a member of the genus Undibacterium . Digital DNA–DNA hybridization and average nucleotide identity analyses were performed and the values between strain B2R-29T and its closely related Undibacterium species were less than 75.1 % and 16.9 %, respectively. The chemotaxonomic data of B2R-29T were as follows: major uniquinone, Q-8; predominant polar lipids, phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol; major fatty acids, C16 : 0 and summed feature 3 (C16 : 1  ω7c / C16 : 1  ω6c); predominant polyamines, putrescine, 2-hydroxyputrescine and spermidine. The DNA G+C content was 51.7 mol% from the genomic sequencing data. In accordance with the phenotypic, physiological and chemotaxonomic properties mentioned above, strain B2R-29T represents a novel species of the genus Undibacterium for which the name Undibacterium crateris sp. nov. is proposed. The type strain is B2R-29T (=CGMCC 1.13792T=KCTC 72018T).

Paenibacillus tianjinensis sp. nov., isolated from corridor air

2021 ◽  
Vol 71 (12) ◽  
Author(s):  
Hongxiang Liu ◽  
Lijing Lu ◽  
Sijin Wang ◽  
Meng Yu ◽  
Xiaoyun Cao ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Major Fatty Acid ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Unidentified Phospholipid ◽  
Content Type ◽  
Link Type ◽  
Pr China ◽  
Type Strains

A Gram-stain-positive, facultatively anaerobic, non-motile, endospore-forming and rod-shaped bacterium, occurring singly or in pairs, designated TB2019T, was isolated from environmental monitoring samples of corridor air collected at the Tianjin Institute for Drug Control, Tianjin Province (PR China). The isolate was able to grow at 15–40 °C (optimum growth at 37 °C), pH 6.0–8.0 (pH 7.0) and in the presence of 0–2% (w/v) NaCl (0% NaCl). Comparison of 16S rRNA gene sequences indicated that TB2019T was most closely related to Paenibacillus typhae CGMCC 1.11012T (98.63%), Paenibacillus albidus Q4-3T (98.19%), Paenibacillus borealis DSM 13188T (97.55%), Paenibacillus helianthi P26ET (97.33%) and Paenibacillus odorifer DSM 15391T (97.19%). The digital DNA–DNA hybridization and the average nucleotide identity values between TB2019T and the five type strains mentioned above ranged from 20.7 to 25.0% and 75.2 to 81.3%, respectively, and the genomic DNA G+C content was 49.52 mol%. The diagnostic cell-wall sugar was ribose, and the diagnostic amino acid was meso-diaminopimelic acid. The polar lipids of TB2019T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified aminophospholipids and one unidentified phospholipid. MK-7 was the predominant menaquinone, and anteiso-C15:0 (30.6%) was the major fatty acid. Based on the polyphasic taxonomic data, strain TB2019T represents a novel species of the genus Paenibacillus , for which the name Paenibacillus tianjinensis sp. nov. is proposed. The type strain is TB2019T (=CICC 25065T=JCM 34610T).

scholarly journals Streptococcus chenjunshii sp. nov. isolated from feces of Tibetan antelopes

2019 ◽  
Vol 69 (4) ◽  
pp. 1237-1243 ◽  
Author(s):  
Zhi Tian ◽  
Shan Lu ◽  
Dong Jin ◽  
Jing Yang ◽  
Ji Pu ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Tibet Plateau ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Molecular Phylogenetic ◽  
Pr China ◽  
Qinghai Tibet Plateau ◽  
Core Genes

Three Gram-stain-positive, catalase-negative, α-haemolytic, chain-forming and coccus-shaped microorganisms (strains Z15T, Z1 and Z2) were isolated from feces of Tibetan antelopes collected from the Qinghai–Tibet plateau, PR China. The results of 16S rRNA gene sequence studies indicated that Z15T shared 94.5, 93.1 and 92.2 % similarity with Streptococcus pantholopis DSM 102135T, Streptococcus ursoris NUM 1615T and Streptococcus dentapri NUM 1529T, respectively. rpoB and groEL-based sequence analysis of our three novel isolates revealed interspecies divergence of 16.7 and 14.3 % from Streptococcus pantholopis DSM 102135T. The genomic DNA G+C content of Z15T is 42.48 mol%. Z15T has an average nucleotide identity (ANI) value of 81.19 % with S. pantholopis DSM 102135T and a DNA–DNA relatedness value of less than 70 % in the in-silico DNA–DNA hybridization (isDDH) with other species of genus Streptococcus deposited in the GenBank database. A whole-genome phylogenetic tree based on 246 core genes of 78 genomes of members of the genus Streptococcus indicated that Z15T represents a member of genus Streptococcus but one well separated from the currently recognized species. Z15T contains C18 : 1ω7c (25.5 %), C18 : 1ω9c (19.6 %), C16 : 0 (17.5 %) and C16 : 1ω9c (13.3 %) as its major cellular fatty acids. According to the morphological, biochemical and molecular phylogenetic features of the three novel isolates, they represent a novel species of the genus Streptococcus , and Streptococcus chenjunshii sp. nov. is thus proposed. The type strain is Z15T (=CGMCC 1.16529=DSM 106182).

scholarly journals Cyclobacterium qasimii sp. nov., a psychrotolerant bacterium isolated from Arctic marine sediment

2012 ◽  
Vol 62 (Pt_9) ◽  
pp. 2133-2139 ◽  
Author(s):  
S. Shivaji ◽  
P. Vishnu Vardhan Reddy ◽  
S. S. S. Nageshwara Rao ◽  
Zareena Begum ◽  
Poorna Manasa ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Marine Sediment ◽  
Dna Hybridization ◽  
Type Species ◽  
Unsaturated Fatty Acids ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Sequence Similarities

A novel Gram-stain-negative, horseshoe-shaped, non-motile bacterium, designated strain M12-11BT, was isolated from a marine sediment sample collected at a depth of 200 m from Kongsfjorden, Svalbard. The colony colour was orangish red due to the presence of carotenoids. Fatty acids were dominated by branched and unsaturated fatty acids (90.8 %), with a high abundance of iso-C15 : 0 (14.9 %), anteiso-C15 : 0 (11.4 %), iso-C15 : 1 G (13.1 %), C15 : 1ω6c (5.4 %), C17 : 1ω6c (6.7 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 9.3 %) and summed feature 9 (10-methyl C16 : 0 and/or iso-C17 : 1ω9c; 5.9 %). Strain M12-11BT contained MK-7 as the major respiratory quinone. The polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, one unidentified aminolipid and three unidentified lipids. Based on 16S rRNA gene sequence similarities, the type strains of Cyclobacterium amurskyense , Cyclobacterium marinum and Cyclobacterium lianum were most closely related to M12-11BT with sequence similarities of 98.2, 96.8 and 93.3 %, respectively. Other members of the family Cyclobacteriaceae had sequence similarities of <92.0 %. However, DNA–DNA hybridization with Cyclobacterium amurskyense KCTC 12363T and Cyclobacterium marinum DSM 745T showed relatedness values of only 24.5 and 32.5 % with respect to strain M12-11BT. Based on the results of DNA–DNA hybridization experiments and phenotypic and chemotaxonomic data, it appears that strain M12-11BT represents a novel species of the genus Cyclobacterium , for which the name Cyclobacterium qasimii sp. nov. is proposed; the type strain is M12-11BT ( = KCTC 23011T = NBRC 106168T) and it has a DNA G+C content of 40.5 mol%.

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