scholarly journals Detection of Legionella DNA by PCR of whole-blood samples in a mouse model

2003 ◽  
Vol 52 (4) ◽  
pp. 325-329 ◽  
Author(s):  
S. Aoki ◽  
Y. Hirakata ◽  
Y. Miyazaki ◽  
K. Izumikawa ◽  
K. Yanagihara ◽  
...  
Keyword(s):  
Mouse Model ◽  
Legionella Pneumophila ◽  
Detection System ◽  
Lavage Fluid ◽  
Rrna Gene ◽  
Blood Samples ◽  
Non Invasive ◽  
Invasive Method ◽  
Dna Fragment ◽  
Legionella Micdadei

A detection system for Legionella DNA in blood samples based on the PCR was developed and evaluated in A/J mice with experimentally induced Legionella pneumonia. Primers were designed to amplify a 106 bp DNA fragment of the 16S rRNA gene specific to Legionella species. The PCR system could detect clinically relevant Legionella species including Legionella pneumophila, Legionella micdadei, Legionella bozemanae, Legionella dumoffii, Legionella longbeachae, Legionella gormanii and Legionella jordanis. The sensitivity of the PCR system was 20 fg extracted DNA. In the mouse model, the blood PCR was compared with results obtained by PCR on bronchoalveolar lavage fluid (BALF) samples, cultures of blood and BALF and detection of Legionella urinary antigen. Blood PCR was positive until 8 days after infection, while BALF PCR became negative on day 4. These results indicate that PCR using blood samples may be a useful, convenient and non-invasive method for the diagnosis of Legionella pneumonia.

scholarly journals Non-Invasive Blood Glucose Measurement Technique for Diabetic Patients

2020 ◽  
Vol 9 (3) ◽  
pp. 4341-4345
Keyword(s):  
Blood Glucose ◽  
Near Infrared ◽  
Chemical Substance ◽  
Blood Glucose Measurement ◽  
Glucose Measurement ◽  
Diabetic Patients ◽  
Blood Samples ◽  
Non Invasive ◽  
Invasive Method ◽  
Precise Quantification

Diabetes mellitus is one of the most spreading diseases prevalent in the world. The Diabetic patients where in desideratum of monitoring their blood glucose level conventionally for a certain periodic of time. If they were nescient of their checkup it may lead to sundry symptoms like the feel profoundly slothful, tired etc. So they require for a periodic check up without fail. In clinic the prefer syringes for amassing the samples,in some worst cases there will be a possibility of unhydrated syringes . For checking they were in desideratum of a modicum of blood samples. For a precise quantification they will integrate some chemical substance to detect the value. While taking blood samples they feel so uncomfortable, pain and if the syringes where already used are not felicitously sterilized it may lead to sundry disease. The another method for quantifying the sample is a non-invasive. In non-invasive method there will be no desideratum of any syringe. It will be pain less and withal comfortable for the patients. In this paper we are introducing a non-invasive method in lieu of invasive method to be liberate from pain. Our proposed system consists of near infrared transmitter and receiver , a light dependent resistor etc,. The rays are sanctioned to passes through the finger it will quantify the glucose present in our cell and the output is given to the processor. The processed output will determine the quantity of glucose present in the blood . Then determinately the obtained output will be exhibited in the Liquid Crystal Exhibit.

scholarly journals Examination the stresseffect of litter’s let-together system (“kindergarten”) by measuring faecal cortisol metabolites of sows and piglets

2016 ◽  
pp. 87-90
Author(s):  
Zsolt Győri ◽  
Margit Kulcsár ◽  
Péter Balogh ◽  
László Huzsvai ◽  
Gabriella Novotniné Dankó
Keyword(s):  
Stress Effect ◽  
Sample Collection ◽  
Blood Samples ◽  
Adrenocortical Activity ◽  
Non Invasive ◽  
Invasive Method ◽  
Corticosterone Metabolites ◽  
Pig Husbandry ◽  
Cortisol Metabolites

Piglets in commercial intensive pig husbandry are often abruptly weaned between 3 and 4 weeks for economic reasons. The process of weaning is a multifactorial stressor, in which nutritional, social, physical and psychologic stressors are combined. Piglets are often exposed to unfamiliar piglets around weaning which results in a period of vigorous fighting. Stress plays an important part in welfare research. Traditionally glucocorticoids are measured in blood samples but their use is often limited as the act of sample collection may stress an animal. Measurement of faecal cortisol/corticosterone metabolites is a non-invasive method for evaluation adrenocortical activity. The aim of this study was to examine the effect of litter’s let-together system (“kindergarten”) in the farrowing house by measuring faecal cortisol metabolites. According to our results the “kindergarten” system has no stress effect on sows and piglets, respectively.

scholarly journals Comparative Evaluation Of Two Techniques Of Sex Determination In Lovebirds (Agapornis Spp.)

2020 ◽  
Vol 77 (2) ◽  
pp. 106
Author(s):  
Maria Carmen TURCU ◽  
Lucia Victoria BEL ◽  
Tommaso COLLARILE ◽  
Dana Liana PUSTA
Keyword(s):  
Sex Determination ◽  
Comparative Evaluation ◽  
Surgical Instruments ◽  
Dna Testing ◽  
Blood Samples ◽  
Non Invasive ◽  
Dna Sampling ◽  
Invasive Method ◽  
Pcr Method ◽  
Materials Used

Most lovebirds (Agapornis spp.) have no distinct sexual dimorphic traits. The objective of the paper was to compare the results of two sexing methods, surgical sexing by celioscopy and DNA sexing by PCR from blood samples, in order to evaluate their effectiveness. Materials used to carry out the studies were a 2.7 mm telescope and endoscopy unit, surgical instruments and DNA sampling kits provided by Exomed.cz laboratory. Forty-two lovebirds were included in this study. The endoscopic sexing procedure was performed by the method described by Divers. Blood sampling for DNA testing by PCR method was performed from the metatarsal vein. The results were different in the case of one lovebird, endoscopic sexing revealed it as male, compared to DNA testing, where it was identified as female. DNA sexing is a non-invasive method that might be more accurate than celioscopy in this species, and bird owners have easier access to it.

scholarly journals Combined use of real-time PCR and nested sequence-based typing in survey of humanLegionellainfection

2016 ◽  
Vol 144 (9) ◽  
pp. 2006-2010 ◽  
Author(s):  
T. QIN ◽  
H. ZHOU ◽  
H. REN ◽  
W. SHI ◽  
H. JIN ◽  
...  
Keyword(s):  
Real Time ◽  
Legionella Pneumophila ◽  
Real Time Pcr ◽  
Pcr Amplification ◽  
Lavage Fluid ◽  
Rrna Gene ◽  
Culture Methods ◽  
Combined Use ◽  
Antigen Tests ◽  
Nested Sequence

SUMMARYLegionnaires’ disease (LD) is a globally distributed systemic infectious disease. The burden of LD in many regions is still unclear, especially in Asian countries including China. A survey ofLegionellainfection using real-time PCR and nested sequence-based typing (SBT) was performed in two hospitals in Shanghai, China. A total of 265 bronchoalveolar lavage fluid (BALF) specimens were collected from hospital A between January 2012 and December 2013, and 359 sputum specimens were collected from hospital B throughout 2012. A total of 71 specimens were positive forLegionellaaccording to real-time PCR focusing on the 5S rRNA gene. Seventy of these specimens were identified asLegionella pneumophilaas a result of real-time PCR amplification of thedotA gene. Results of nested SBT revealed high genetic polymorphism in theseL. pneumophilaand ST1 was the predominant sequence type. These data revealed that the burden of LD in China is much greater than that recognized previously, and real-time PCR may be a suitable monitoring technology for LD in large sample surveys in regions lacking the economic and technical resources to perform other methods, such as urinary antigen tests and culture methods.

Malfunctional Reorganization in the Developing Limbo-Prefrontal System in Animals: Implications for Human Psychoses?

2001 ◽  
Vol 12 (1) ◽  
pp. 8-14
Author(s):  
Gertraud Teuchert-Noodt ◽  
Ralf R. Dawirs
Keyword(s):  
Prefrontal Cortex ◽  
Mental Disorders ◽  
Dentate Gyrus ◽  
Cognitive Functions ◽  
Experimental Approach ◽  
Regulatory Mechanisms ◽  
Hippocampal Dentate Gyrus ◽  
Non Invasive ◽  
Invasive Method ◽  
Activity Dependent

Abstract: Neuroplasticity research in connection with mental disorders has recently bridged the gap between basic neurobiology and applied neuropsychology. A non-invasive method in the gerbil (Meriones unguiculus) - the restricted versus enriched breading and the systemically applied single methamphetamine dose - offers an experimental approach to investigate psychoses. Acts of intervening affirm an activity dependent malfunctional reorganization in the prefrontal cortex and in the hippocampal dentate gyrus and reveal the dopamine position as being critical for the disruption of interactions between the areas concerned. From the extent of plasticity effects the probability and risk of psycho-cognitive development may be derived. Advance may be expected from insights into regulatory mechanisms of neurogenesis in the hippocampal dentate gyrus which is obviously to meet the necessary requirements to promote psycho-cognitive functions/malfunctions via the limbo-prefrontal circuit.

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