scholarly journals Molecular and Biological Characterization of a Cryptosporidium molnari-Like Isolate from a Guppy (Poecilia reticulata)

2004 ◽  
Vol 70 (6) ◽  
pp. 3761-3765 ◽  
Author(s):  
Una Ryan ◽  
Amanda O'Hara ◽  
Lihua Xiao
Keyword(s):  
Poecilia Reticulata ◽  
Genetic Characterization ◽  
Phylogenetic Analyses ◽  
Biological Characterization

ABSTRACT Histological, morphological, genetic, and phylogenetic analyses of a Cryptosporidium molnari-like isolate from a guppy (Poecilia reticulata) identified stages consistent with those of C. molnari and revealed that C. molnari is genetically very distinct from all other species of Cryptosporidium. This study represents the first genetic characterization of C. molnari.

scholarly journals Genetic characterization of a novel picornavirus distantly related to the marine mammal-infecting aquamaviruses in a long-distance migrant bird species, European roller (Coracias garrulus)

2013 ◽  
Vol 94 (9) ◽  
pp. 2029-2035 ◽  
Author(s):  
Ákos Boros ◽  
Tamás Kiss ◽  
Orsolya Kiss ◽  
Péter Pankovics ◽  
Beatrix Kapusinszky ◽  
...  
Keyword(s):  
Marine Mammal ◽  
Genetic Characterization ◽  
Phylogenetic Analyses ◽  
Bird Species ◽  
Long Distance ◽  
Coracias Garrulus ◽  
Faecal Samples ◽  
European Roller ◽  
Migrant Bird

Despite the continuously growing number of known avian picornaviruses (family Picornaviridae), knowledge of their genetic diversity in wild birds, especially in long-distance migrant species is very limited. In this study, we report the presence of a novel picornavirus identified from one of 18 analysed faecal samples of an Afro-Palearctic migrant bird, the European roller (Coracias garrulus L., 1758), which is distantly related to the marine-mammal-infecting seal aquamavirus A1 (genus Aquamavirus). The phylogenetic analyses and the low sequence identity (P1 26.3 %, P2 25.8 % and P3 28.4 %) suggest that this picornavirus could be the founding member of a novel picornavirus genus that we have provisionally named ‘Kunsagivirus’, with ‘Greplavirus A’ (strain roller/SZAL6-KuV/2011/HUN, GenBank accession no. KC935379) as the candidate type species.

scholarly journals Genetic Characterization of Vibrio vulnificus Strains from Tilapia Aquaculture in Bangladesh

2010 ◽  
Vol 76 (14) ◽  
pp. 4890-4895 ◽  
Author(s):  
Zahid H. Mahmud ◽  
Anita C. Wright ◽  
Shankar C. Mandal ◽  
Jianli Dai ◽  
Melissa K. Jones ◽  
...  
Keyword(s):  
Vibrio Vulnificus ◽  
Genetic Characterization ◽  
Phylogenetic Analyses ◽  
Wound Infections ◽  
Freshwater Aquaculture

ABSTRACT Outbreaks of Vibrio vulnificus wound infections in Israel were previously attributed to tilapia aquaculture. In this study, V. vulnificus was frequently isolated from coastal but not freshwater aquaculture in Bangladesh. Phylogenetic analyses showed that strains from Bangladesh differed remarkably from isolates commonly recovered elsewhere from fish or oysters and were more closely related to strains of clinical origin.

scholarly journals Identification and Genetic Characterization of a Novel Respirovirus in Alpine Chamois (Rupicapra rupicapra rupicapra)

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 704
Author(s):  
Camilla Luzzago ◽  
Erika Ebranati ◽  
Antonio Lavazza ◽  
Martina Besozzi ◽  
Gianguglielmo Zehender ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Phylogenetic Analyses ◽  
Amino Acid Sequences ◽  
Species Demarcation Criterion ◽  
Demarcation Criterion ◽  
Protein Amino Acid ◽  
Rupicapra Rupicapra ◽  
Separate Branch ◽  
Alpine Chamois

The Respirovirus genus, family Paramamixoviridae, includes respiratory viral pathogens. Here we report the identification and genetic characterization of a respirovirus in an Alpine chamois showing interstitial pneumonia associated with catarrhal bronchopneumonia. The full-genome characterization of this respirovirus, named ChamoisRV/IT2014, revealed low similarities to caprine respirovirus (77.1%), bovine respirovirus (74.5%) and human respirovirus (72.0%). The phylogenetic analyses based on the full-length genome sequence of the novel isolate and reference respirovirus strains showed that ChamoisRV/IT2014 clustered with caprine respirovirus but formed a separate branch. The phylogenetic tree topology of complete large protein amino acid sequences, representing the current species demarcation criterion for Respirovirus genus, showed a 0.05 branch length of ChamoisRV/IT2014 sequence between the nearest node and the tip of the branch, suggesting that this virus belongs to a novel species. This new isolate in a new host species raises several questions to be addressed on the epidemiological role of chamois and the risks of cross-transmission between wild ruminants and livestock.

scholarly journals Genetic characterization of a novel duck-origin picornavirus with six 2A proteins

2014 ◽  
Vol 95 (6) ◽  
pp. 1289-1296 ◽  
Author(s):  
Xiaoyan Wang ◽  
Ning Liu ◽  
Fumin Wang ◽  
Kang Ning ◽  
Yanbo Li ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Genetic Characterization ◽  
Hepatitis A Virus ◽  
Phylogenetic Analyses ◽  
Entry Site ◽  
Internal Ribosome Entry ◽  
Founding Member ◽  
Duck Hepatitis ◽  
Novel Virus

A novel virus was detected from diseased ducks and completely determined. The virus was shown to have a picornavirus-like genome layout. Interestingly, the genome contained a total of up to six 2As, including four 2As (2A1–2A4) each having an NPGP motif, an AIG1-like 2A5, and a parechovirus-like 2A6. The 5′UTR was predicted to possess a hepacivirus/pestivirus-like internal ribosome entry site (IRES). However, the subdomain IIIe consisted of a 3 nt stem and five unpaired bases, distinct from those found in all other HP-like IRESs. The virus was most closely related to duck hepatitis A virus, with amino acid identities of 37.7 %, 39 % and 43.7 % in the P1, P2 and P3 regions, respectively. Based on these investigations, together with phylogenetic analyses, the virus could be considered as the founding member of a novel picornavirus genus that we tentatively named ‘Aalivirus’, with ‘Aalivirus A’ as the type species.

Isolation and Molecular Characterization of Turkey Pox Virus from Maharashtra, India: A Review

2021 ◽  
Author(s):  
M.M. Pawade ◽  
P.P. Mhase ◽  
D.M. Muglikar ◽  
V.D. Lonkar ◽  
P.V. Mehere ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Virus Isolation ◽  
Viral Infections ◽  
Genetic Characterization ◽  
Phylogenetic Analyses ◽  
Chain Reaction ◽  
Slow Spreading ◽  
Polymerase Chain ◽  
Avian Pox

Avian pox diseases are contagious and slow spreading viral infections in birds. The present study was aim to, isolate and molecular characterization of turkeypox virus from a clinical case. Ten out of the twelve scab lesions sample collected from clinically suspected cases were positive for avian pox viurs (APV) based on virus isolation and polymerase chain reaction. We conducted genetic characterization of the APV strain. The phylogenetic analyses of P4b gene APV genome indicated that, avian poxviruses fragments sequenced in this study clustered along the A clade of avipoxviruses, genetically related to Indian fowl pox virus isolated from chicken, showing 99% homology.

scholarly journals Isolation and Genetic Characterization of New Reassortant H3N1 Swine Influenza Virus from Pigs in the Midwestern United States

2006 ◽  
Vol 80 (10) ◽  
pp. 5092-5096 ◽  
Author(s):  
Wenjun Ma ◽  
Marie Gramer ◽  
Kurt Rossow ◽  
Kyoung-Jin Yoon
Keyword(s):  
United States ◽  
Influenza Virus ◽  
Genetic Characterization ◽  
Phylogenetic Analyses ◽  
Swine Influenza Virus ◽  
Swine Influenza ◽  
Influenza Viruses ◽  
Virus Origin ◽  
Reassortant Viruses

ABSTRACT Since the introduction of H3N2 swine influenza viruses (SIVs) into U.S. swine in 1998, H1N2 and H1N1 reassortant viruses have emerged from reassortment between classical H1N1 and H3N2 viruses. In 2004, a new reassortant H3N1 virus (A/Swine/Minnesota/00395/2004) was identified from coughing pigs. Phylogenetic analyses revealed a hemagglutinin segment similar to those of contemporary cluster III H3N2 SIVs and a neuraminidase sequence of contemporary H1N1 origin. The internal genes were of swine, human, and avian influenza virus origin, similar to those of contemporary U.S. cluster III H3N2 SIVs. The recovery of H3N1 is further evidence of reassortment among SIVs and justifies continuous surveillance.

Histopathological and genetic characterization of colorectal mucinous carcinoma

2001 ◽  
Vol 120 (5) ◽  
pp. A166-A166
Author(s):  
S FUJII ◽  
T KUSAKA ◽  
T KAIHARA ◽  
Y UEDA ◽  
T CHIBA ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Mucinous Carcinoma

Genetic characterization of high hyperdiploidy in childhood acute lymphoblastic leukemia

2009 ◽  
Vol 221 (03) ◽  
Author(s):  
R Vagkopoulou ◽  
C Eckert ◽  
U Ungethüm ◽  
G Körner ◽  
M Stanulla ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Genetic Characterization ◽  
Lymphoblastic Leukemia ◽  
Childhood Acute Lymphoblastic Leukemia

TBE in Sweden

2020 ◽  
Keyword(s):  
Genetic Characterization ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Second Phase ◽  
Serum Samples ◽  
Tick Borne Encephalitis ◽  
Specific Immunoglobulin ◽  
Tick Borne Encephalitis Virus ◽  
First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

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