Genetic Characterization of Vibrio vulnificus Strains from Tilapia Aquaculture in Bangladesh

ABSTRACT Outbreaks of Vibrio vulnificus wound infections in Israel were previously attributed to tilapia aquaculture. In this study, V. vulnificus was frequently isolated from coastal but not freshwater aquaculture in Bangladesh. Phylogenetic analyses showed that strains from Bangladesh differed remarkably from isolates commonly recovered elsewhere from fish or oysters and were more closely related to strains of clinical origin.

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Genetic characterization of a novel picornavirus distantly related to the marine mammal-infecting aquamaviruses in a long-distance migrant bird species, European roller (Coracias garrulus)

Journal of General Virology ◽

10.1099/vir.0.054676-0 ◽

2013 ◽

Vol 94 (9) ◽

pp. 2029-2035 ◽

Cited By ~ 19

Author(s):

Ákos Boros ◽

Tamás Kiss ◽

Orsolya Kiss ◽

Péter Pankovics ◽

Beatrix Kapusinszky ◽

...

Keyword(s):

Marine Mammal ◽

Genetic Characterization ◽

Phylogenetic Analyses ◽

Bird Species ◽

Long Distance ◽

Coracias Garrulus ◽

Faecal Samples ◽

European Roller ◽

Migrant Bird

Despite the continuously growing number of known avian picornaviruses (family Picornaviridae), knowledge of their genetic diversity in wild birds, especially in long-distance migrant species is very limited. In this study, we report the presence of a novel picornavirus identified from one of 18 analysed faecal samples of an Afro-Palearctic migrant bird, the European roller (Coracias garrulus L., 1758), which is distantly related to the marine-mammal-infecting seal aquamavirus A1 (genus Aquamavirus). The phylogenetic analyses and the low sequence identity (P1 26.3 %, P2 25.8 % and P3 28.4 %) suggest that this picornavirus could be the founding member of a novel picornavirus genus that we have provisionally named ‘Kunsagivirus’, with ‘Greplavirus A’ (strain roller/SZAL6-KuV/2011/HUN, GenBank accession no. KC935379) as the candidate type species.

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Prevalence and Genetic Characterization of Vibrio vulnificus in Raw Seafood and Seawater in Malaysia

Journal of Food Protection ◽

10.4315/0362-028x.jfp-13-141 ◽

2013 ◽

Vol 76 (10) ◽

pp. 1797-1800 ◽

Cited By ~ 6

Author(s):

MOHAMMADJAVAD PAYDAR ◽

KWAI LIN THONG

Keyword(s):

Coastal Waters ◽

Vibrio Vulnificus ◽

Genetic Characterization ◽

Human Pathogen ◽

Enterobacterial Repetitive Intergenic Consensus ◽

High Genetic Diversity ◽

Pcr Assays ◽

Seawater Samples ◽

Repetitive Extragenic Palindromic Pcr

Vibrio vulnificus is a highly invasive human pathogen that exists naturally in estuarine environment and coastal waters. In this study, we used different PCR assays to detect V. vulnificus in 260 seafood and 80 seawater samples. V. vulnificus was present in about 34 (13%) of the 260 seafood samples and 18 (23%) of the 80 seawater samples. Repetitive extragenic palindromic PCR (REP-PCR) and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) were applied to subtype the V. vulnificus isolates. Twenty-five REP profiles and 45 ERIC profiles were observed, and the isolates were categorized into 9 and 10 distinct clusters at the similarity of 80%, by REP-PCR and ERIC-PCR, respectively. ERIC-PCR is more discriminative than REP-PCR in subtyping V. vulnificus, demonstrating high genetic diversity among the isolates.

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Cloning and Characterization of a Periplasmic Nuclease of Vibrio vulnificus and Its Role in Preventing Uptake of Foreign DNA

Applied and Environmental Microbiology ◽

10.1128/aem.67.1.82-88.2001 ◽

2001 ◽

Vol 67 (1) ◽

pp. 82-88 ◽

Cited By ~ 39

Author(s):

Shi-I Wu ◽

Shi-Kan Lo ◽

Chung-Ping Shao ◽

Hsing-Wen Tsai ◽

Lien-I Hor

Keyword(s):

Parent Strain ◽

Vibrio Vulnificus ◽

Signal Sequence ◽

Reduced Form ◽

Wound Infections ◽

Reading Frame ◽

E Coli ◽

Dna And Rna ◽

Foreign Dna

ABSTRACT We have cloned a nuclease gene, vvn, from Vibrio vulnificus, an estuarine bacterium that causes wound infections and septicemia in humans and eels. The gene contained a 696-bp open reading frame encoding 232 amino acids (aa), including a signal sequence of 18 aa. The deduced amino acid sequence of the mature nuclease predicted a molecular mass of 25 kDa, which was confirmed by vital stain, and a pI of 8.6. Vvn was produced in the periplasm of either V. vulnificus or recombinant Escherichia coli strains and was active in the oxidized (but not the reduced) form. This nuclease was able to digest DNA and RNA, with differential thermostability in DNase and RNase activities. Expression of Vvn inE. coli DH5α reduced the frequencies of transformation with the divalent ion-treated cells and electroporation by about 6 and 2 logs, respectively. In addition, the transformation frequency of a Vvn-deficient V. vulnificus mutant (ND) was 10-fold higher than that of the parent strain. These data suggested that Vvn may be involved in preventing uptake of foreign DNA by transformation. However, Vvn expressed in the recipients had little effect on the conjugation frequency in either E. coli or V. vulnificus. Some other DNase(s) may be present in the periplasm and responsible for a residual DNase activity, which was about one-fourth of that of the parent strain, detected in the ND mutant. We also demonstrated that Vvn was not required for the virulence ofV. vulnificus mice.

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Identification and Genetic Characterization of a Novel Respirovirus in Alpine Chamois (Rupicapra rupicapra rupicapra)

Animals ◽

10.3390/ani10040704 ◽

2020 ◽

Vol 10 (4) ◽

pp. 704

Author(s):

Camilla Luzzago ◽

Erika Ebranati ◽

Antonio Lavazza ◽

Martina Besozzi ◽

Gianguglielmo Zehender ◽

...

Keyword(s):

Genetic Characterization ◽

Phylogenetic Analyses ◽

Amino Acid Sequences ◽

Species Demarcation Criterion ◽

Demarcation Criterion ◽

Protein Amino Acid ◽

Rupicapra Rupicapra ◽

Separate Branch ◽

Alpine Chamois

The Respirovirus genus, family Paramamixoviridae, includes respiratory viral pathogens. Here we report the identification and genetic characterization of a respirovirus in an Alpine chamois showing interstitial pneumonia associated with catarrhal bronchopneumonia. The full-genome characterization of this respirovirus, named ChamoisRV/IT2014, revealed low similarities to caprine respirovirus (77.1%), bovine respirovirus (74.5%) and human respirovirus (72.0%). The phylogenetic analyses based on the full-length genome sequence of the novel isolate and reference respirovirus strains showed that ChamoisRV/IT2014 clustered with caprine respirovirus but formed a separate branch. The phylogenetic tree topology of complete large protein amino acid sequences, representing the current species demarcation criterion for Respirovirus genus, showed a 0.05 branch length of ChamoisRV/IT2014 sequence between the nearest node and the tip of the branch, suggesting that this virus belongs to a novel species. This new isolate in a new host species raises several questions to be addressed on the epidemiological role of chamois and the risks of cross-transmission between wild ruminants and livestock.

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Molecular and Biological Characterization of a Cryptosporidium molnari-Like Isolate from a Guppy (Poecilia reticulata)

Applied and Environmental Microbiology ◽

10.1128/aem.70.6.3761-3765.2004 ◽

2004 ◽

Vol 70 (6) ◽

pp. 3761-3765 ◽

Cited By ~ 30

Author(s):

Una Ryan ◽

Amanda O'Hara ◽

Lihua Xiao

Keyword(s):

Poecilia Reticulata ◽

Genetic Characterization ◽

Phylogenetic Analyses ◽

Biological Characterization

ABSTRACT Histological, morphological, genetic, and phylogenetic analyses of a Cryptosporidium molnari-like isolate from a guppy (Poecilia reticulata) identified stages consistent with those of C. molnari and revealed that C. molnari is genetically very distinct from all other species of Cryptosporidium. This study represents the first genetic characterization of C. molnari.

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Genetic characterization of a novel duck-origin picornavirus with six 2A proteins

Journal of General Virology ◽

10.1099/vir.0.063313-0 ◽

2014 ◽

Vol 95 (6) ◽

pp. 1289-1296 ◽

Cited By ~ 15

Author(s):

Xiaoyan Wang ◽

Ning Liu ◽

Fumin Wang ◽

Kang Ning ◽

Yanbo Li ◽

...

Keyword(s):

Hepatitis A ◽

Genetic Characterization ◽

Hepatitis A Virus ◽

Phylogenetic Analyses ◽

Entry Site ◽

Internal Ribosome Entry ◽

Founding Member ◽

Duck Hepatitis ◽

Novel Virus

A novel virus was detected from diseased ducks and completely determined. The virus was shown to have a picornavirus-like genome layout. Interestingly, the genome contained a total of up to six 2As, including four 2As (2A1–2A4) each having an NPGP motif, an AIG1-like 2A5, and a parechovirus-like 2A6. The 5′UTR was predicted to possess a hepacivirus/pestivirus-like internal ribosome entry site (IRES). However, the subdomain IIIe consisted of a 3 nt stem and five unpaired bases, distinct from those found in all other HP-like IRESs. The virus was most closely related to duck hepatitis A virus, with amino acid identities of 37.7 %, 39 % and 43.7 % in the P1, P2 and P3 regions, respectively. Based on these investigations, together with phylogenetic analyses, the virus could be considered as the founding member of a novel picornavirus genus that we tentatively named ‘Aalivirus’, with ‘Aalivirus A’ as the type species.

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Isolation and Molecular Characterization of Turkey Pox Virus from Maharashtra, India: A Review

Indian Journal of Animal Research ◽

10.18805/ijar.b-4468 ◽

2021 ◽

Author(s):

M.M. Pawade ◽

P.P. Mhase ◽

D.M. Muglikar ◽

V.D. Lonkar ◽

P.V. Mehere ◽

...

Keyword(s):

Molecular Characterization ◽

Virus Isolation ◽

Viral Infections ◽

Genetic Characterization ◽

Phylogenetic Analyses ◽

Chain Reaction ◽

Slow Spreading ◽

Polymerase Chain ◽

Avian Pox

Avian pox diseases are contagious and slow spreading viral infections in birds. The present study was aim to, isolate and molecular characterization of turkeypox virus from a clinical case. Ten out of the twelve scab lesions sample collected from clinically suspected cases were positive for avian pox viurs (APV) based on virus isolation and polymerase chain reaction. We conducted genetic characterization of the APV strain. The phylogenetic analyses of P4b gene APV genome indicated that, avian poxviruses fragments sequenced in this study clustered along the A clade of avipoxviruses, genetically related to Indian fowl pox virus isolated from chicken, showing 99% homology.

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Isolation and Genetic Characterization of New Reassortant H3N1 Swine Influenza Virus from Pigs in the Midwestern United States

Journal of Virology ◽

10.1128/jvi.80.10.5092-5096.2006 ◽

2006 ◽

Vol 80 (10) ◽

pp. 5092-5096 ◽

Cited By ~ 64

Author(s):

Wenjun Ma ◽

Marie Gramer ◽

Kurt Rossow ◽

Kyoung-Jin Yoon

Keyword(s):

United States ◽

Influenza Virus ◽

Genetic Characterization ◽

Phylogenetic Analyses ◽

Swine Influenza Virus ◽

Swine Influenza ◽

Influenza Viruses ◽

Virus Origin ◽

Reassortant Viruses

ABSTRACT Since the introduction of H3N2 swine influenza viruses (SIVs) into U.S. swine in 1998, H1N2 and H1N1 reassortant viruses have emerged from reassortment between classical H1N1 and H3N2 viruses. In 2004, a new reassortant H3N1 virus (A/Swine/Minnesota/00395/2004) was identified from coughing pigs. Phylogenetic analyses revealed a hemagglutinin segment similar to those of contemporary cluster III H3N2 SIVs and a neuraminidase sequence of contemporary H1N1 origin. The internal genes were of swine, human, and avian influenza virus origin, similar to those of contemporary U.S. cluster III H3N2 SIVs. The recovery of H3N1 is further evidence of reassortment among SIVs and justifies continuous surveillance.

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