The Antiapoptotic RBM5/LUCA-15/H37 Gene and Its Role in Apoptosis and Human Cancer: Research Update

The candidate tumour-suppressor gene, LUCA-15/RBM5/H37, maps to the lung cancer tumour-suppressor locus 3p21.3. The LUCA-15 gene locus encodes at least four alternatively spliced transcripts that have been shown to function as regulators of apoptosis, a fact which may have major significance in tumour regulation. This review highlights recent evidence that further implicates the LUCA-15 locus in the control of apoptosis and cell proliferation, and focuses on the observations that confirm the tumour-suppressor activity of this gene.

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RBM5/LUCA-15 — Tumour Suppression by Control of Apoptosis and the Cell Cycle?

The Scientific World JOURNAL ◽

10.1100/tsw.2002.859 ◽

2002 ◽

Vol 2 ◽

pp. 1885-1890 ◽

Cited By ~ 8

Author(s):

Mirna Mourtada-Maarabouni ◽

Gwyn T. Williams

Keyword(s):

Gene Locus ◽

Suppressor Gene ◽

Tumour Suppressor Gene ◽

Tumour Suppressor ◽

Suppressor Activity ◽

Tumour Suppression ◽

Major Significance ◽

Tumour Suppressor Activity ◽

Alternatively Spliced ◽

Candidate Tumour Suppressor

The candidate tumour suppressor gene, LUCA-15, maps to the lung cancer tumour suppressor locus 3p21.3. The LUCA-15 gene locus encodes at least four alternatively spliced transcripts, which have been shown to function as regulators of apoptosis, a fact that may have a major significance in tumour regulation. This review highlights evidence that implicates the LUCA-15 locus in the control of apoptosis and cell proliferation, and reports observations that significantly strengthen the case for tumour suppressor activity by this gene.

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PTEN: A molecular target for neurodegenerative disorders

Translational Neuroscience ◽

10.2478/s13380-012-0018-9 ◽

2012 ◽

Vol 3 (2) ◽

Cited By ~ 6

Author(s):

Azza Ismail ◽

Ke Ning ◽

Abdulmonem Al-Hayani ◽

Basil Sharrack ◽

Mimoun Azzouz

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Neurodegenerative Disorders ◽

Neuronal Development ◽

Suppressor Gene ◽

Tumour Suppressor Gene ◽

Tumour Suppressor ◽

Suppressor Activity ◽

Tumour Suppressor Activity ◽

The Central Nervous System

AbstractPTEN (phosphatase and tensin homologue deleted in chromosome 10) was first identified as a candidate tumour suppressor gene located on chromosome 10q23. It is considered as one of the most frequently mutated genes in human malignancies. Emerging evidence shows that the biological function of PTEN extends beyond its tumour suppressor activity. In the central nervous system PTEN is a crucial regulator of neuronal development, neuronal survival, axonal regeneration and synaptic plasticity. Furthermore, PTEN has been linked to the pathogenesis of neurodegenerative disorders such as Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis. Recently increased attention has been focused on PTEN as a potential target for the treatment of brain injury and neurodegeneration. In this review we discuss the essential functions of PTEN in the central nervous system and its involvement in neurodegeneration.

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1334 Spleen tyrosine kinase as a novel candidate tumour suppressor gene for human oral squamous cell carcinoma

European Journal of Cancer Supplements ◽

10.1016/s1359-6349(09)70507-5 ◽

2009 ◽

Vol 7 (2) ◽

pp. 148-149

Author(s):

S. Ogane ◽

T. Onda ◽

N. Takano ◽

T. Yajima ◽

T. Uchiyama ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Tyrosine Kinase ◽

Cell Carcinoma ◽

Squamous Cell ◽

Suppressor Gene ◽

Tumour Suppressor Gene ◽

Tumour Suppressor ◽

Spleen Tyrosine Kinase ◽

Candidate Tumour Suppressor

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Characterising Mutational Spectra of Carcinogens in the Tumour Suppressor Gene TP53 Using Human TP53 Knock-in (Hupki) Mouse Embryo Fibroblasts

Methods and Protocols ◽

10.3390/mps2040085 ◽

2019 ◽

Vol 2 (4) ◽

pp. 85 ◽

Cited By ~ 3

Author(s):

Hölzl-Armstrong ◽

Kucab ◽

Korenjak ◽

Luijten ◽

Phillips ◽

...

Keyword(s):

Tp53 Mutation ◽

Human Cancer ◽

Atmospheric Oxygen ◽

Suppressor Gene ◽

Tumour Suppressor Gene ◽

Tumour Suppressor ◽

International Agency ◽

Human Tumours ◽

Clonal Cell Lines ◽

Gene Tp53

DNA in dividing cells is prone to mutagenesis, with mutations making key contributions to human disease including cancer. The tumour suppressor gene TP53 is the most frequently mutated gene in human tumours. Here, we present a robust protocol for studying TP53 mutagenesis utilising human TP53 knock-in (Hupki) mouse embryonic fibroblasts (HUFs). In the HUF immortalisation assay (HIMA), primary HUFs are treated with known or suspected carcinogens at 3% oxygen and then transferred to 20% atmospheric oxygen to induce senescence. Cells containing mutations (e.g., in TP53) that allow bypassing of senescence eventually emerge as immortalised clonal cell lines after 2–3 months of serial passaging. As not all immortalised HUF cells contain TP53 mutations, we developed a Nutlin-3a counter-screen to select for TP53-mutated clones prior to sequencing. TP53 mutation spectra generated can be compared with those of human tumours recorded in the International Agency for Research on Cancer TP53 mutation database. Environmental mutagens that have demonstrated and validated the utility of the HIMA include ultraviolet radiation, aristolochic acid, and benzo[a]pyrene. The TP53 mutation patterns induced by these mutagens in the HIMA corresponded to those found in human tumours from patients exposed to these mutagens. The approach presented helps to deepen our understanding of human cancer aetiology.

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