scholarly journals Evolutionary variations in the biofilm-associated protein BslA from the genus Bacillus

2016 ◽  
Author(s):  
Ryan J. Morris ◽  
Marieke Schor ◽  
Rachel M.C. Gillespie ◽  
Ana Sofia Ferreira ◽  
Keith M. Bromley ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Self Assembly ◽  
Molecular Basis ◽  
Bacillus Amyloliquefaciens ◽  
Bacillus Pumilus ◽  
Water Repellent ◽  
Biophysical Properties ◽  
Genus Bacillus ◽  
Degree Of Protection

AbstractBslA is a protein secreted by Bacillus subtilis which forms a hydrophobic film that coats the biofilm surface and renders it water-repellent. We have characterised three orthologues of BslA from Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus as well as a paralogue from B. subtilis called YweA. We find that the three orthologous proteins can substitute for BslA in B. subtilis and confer a degree of protection, whereas YweA cannot. The degree to which the proteins functionally substitute for native BslA correlates with their in vitro biophysical properties. Our results demonstrate the use of naturally-evolved variants to provide a framework for teasing out the molecular basis of interfacial self-assembly.

Activity of Bacillus amyloliquefaciens MBI600 against soybean bacterioses

2021 ◽  
Author(s):  
R.I. Tarakanov ◽  
◽  
P.A. Vasilyev ◽  
K.S. Troshin ◽  
F.-S. U. Dzhalilov
Keyword(s):  
High Activity ◽  
Bacillus Amyloliquefaciens ◽  
Bacterial Blight ◽  
Genus Bacillus

the article presents data on the effectiveness of biological preparations based on bacteria of the genus Bacillus against pathogens of soybean bacteriosis. The results show high activity of the biofungicide based on Bacillus amyloliquefaciens MBI600 both in vitro and in the vegetative experiment against bacterial blight.

SOME FEATURES OF AN ACETOIN BIOSYNTHESIS BY BACTERIA FROM THE GENUS BACILLUS

2020 ◽  
pp. 390-391
Author(s):  
E. Fedorova ◽  
N. Stoynova
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Amyloliquefaciens ◽  
Genus Bacillus

Several features of an acetoin biosynthesis in Bacillus subtilis and Bacillus amyloliquefaciens were investigated.

Synthesis and Antibacterial Evaluation of Thiouracil Derivatives Contain-ing 1,2,4-Triazolo[1,5-a]Pyrimidine

2020 ◽  
Vol 17 ◽  
Author(s):  
Penglei Cui ◽  
Di Zhang ◽  
Xiumin Guo ◽  
Shujing Ji ◽  
Qingmei Jiang
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Inhibitory Activity ◽  
Bacillus Amyloliquefaciens ◽  
Antibacterial Activities ◽  
Inhibitory Activities ◽  
New Compounds ◽  
Thiouracil Derivatives ◽  
Antibacterial Evaluation

: A series of new thiouracil compounds containing 1,2,4-triazolo[1,5-a]pyrimidine were designed and synthesized. The in vitro antibacterial activities of the new compounds against Bacillus amyloliquefaciens, Staphylococcus aureus and Bacillus subtilis were tested. The results showed that some of the new compounds had strong inhibitory activities against the tested bacteria. At the concentration of 50 μg/mL, the compound 12d had broad and the highest inhibitory activity with the 100% inhibition against the three tested strains, the same as norfloxacin which was used as the control.

scholarly journals Avaliação in vitro da adsorção de aflatoxina B1 por produtos comerciais utilizados na alimentação animal

2017 ◽  
Vol 84 (0) ◽  
Author(s):  
Raizza Eveline Escórcio Pinheiro ◽  
Carina Maricel Pereyra ◽  
Josyanne Araújo Neves ◽  
Rodrigo Maciel Calvet ◽  
Julliet Teixeira de Oliveira Santos ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Bacillus Subtilis ◽  
Bacillus Licheniformis ◽  
Lactobacillus Acidophilus ◽  
Oreochromis Niloticus ◽  
Enterococcus Faecium ◽  
Bacillus Pumilus ◽  
Bifidobacterium Bifidum

RESUMO: Objetivou-se avaliar a capacidade de adsorção in vitro de aflatoxina B1 (AFB1) por produtos comerciais utilizados na alimentação animal. Muitas pesquisas estão sendo realizadas para a descontaminação de AFB1 em alimentos. Os produtos comerciais utilizados frequentemente na alimentação de peixes, disponíveis na forma de probióticos, são formados por cepas de bactérias e leveduras utilizadas na maioria dos ensaios de adsorção de micotoxinas. Foram utilizados três produtos comerciais: A, composto por Bacillus subtilis, Bifidobacterium bifidum, Enterococcus faecium e Lactobacillus acidophilus; B, por leveduras secas de Saccharomyces cerevisiae provenientes de cervejaria; e C, por Bacillus subtilis, Bacillus licheniformis e Bacillus pumilus. Cinco suspensões da dose máxima recomendada pelo fabricante de cada produto (0; 25; 50; 75 e 100%) foram testadas contra AFB1 (1000 ng.mL-1) em microtubos para determinação da capacidade de adsorção. Para simular o pH do estômago e do intestino de tilápias do Nilo (Oreochromis niloticus) foram formuladas soluções tampão fosfato salino (PBS), com pH 1,5 e 7,5; respectivamente. Os microtubos foram introduzidos em uma centrífuga com agitação mecânica, a 37ºC por 1 h e depois centrifugados por 10 min a 14.000 rpm; os sobrenadantes foram quantificados por cromatografia líquida de alta eficiência. Os produtos comerciais, nas concentrações máximas, foram capazes de adsorver AFB1 em quantidades de 45,01 a 129,59; 123,90 a 215,59 e 209,98 a 370,73 ng.mL-1, respectivamente. Concluiu-se que todos os produtos comerciais analisados adsorvem AFB1 em condições simuladas de pH gastrointestinal e são candidatos potenciais para adsorção de AFB1 para futuros ensaios in vivo.

scholarly journals Tunable Extracellular Self-Assembly of Multi-Protein Conjugates from Bacillus subtilis

2016 ◽  
Author(s):  
Charlie Gilbert ◽  
Mark Howarth ◽  
Colin R. Harwood ◽  
Tom Ellis
Keyword(s):  
Bacillus Subtilis ◽  
Self Assembly ◽  
Culture Media ◽  
Protein Complexes ◽  
Modular System ◽  
Protein Conjugation ◽  
Novel Approach ◽  
Multifunctional Enzymes

The ability to stably and specifically conjugate recombinant proteins to one another is a powerful in vitro technique for engineering multifunctional enzymes, protein therapeutics and novel biological materials. However, for many applications spontaneous in vivo protein conjugation would be preferable to in vitro methods. Exploiting the recently described SpyTag-SpyCatcher system, we describe here how enzymes and structural proteins can be genetically-encoded to covalently conjugate in culture media following programmable secretion by Bacillus subtilis. Using this novel approach, we demonstrate how self-conjugation of a secreted industrial enzyme, XynA, dramatically increases its resilience to boiling and we show that cellular consortia can be engineered to self-assemble functional multi-protein complexes with tunable composition. This genetically-encoded modular system provides a new, flexible strategy for protein conjugation harnessing the substantial advantages of extracellular self-assembly.

scholarly journals Antagonistic Activity of Some Plant Growth Rhizobacteria to Fusarium Graminearum

2014 ◽  
Vol 3 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Mohammed Abdulkareem ◽  
Hadi M. Aboud ◽  
Hutham M. Saood ◽  
Mohammed K. Shibly
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Graminearum ◽  
Plant Disease ◽  
Bacillus Pumilus ◽  
Antagonistic Activity ◽  
Azotobacter Chroococcum ◽  
Dual Culture ◽  
In Vitro Technique ◽  
Interaction Test

Biological control is an efficient and environmentally friendly way to prevent plant disease. In vitro technique was used to investigate the ability of Azospirillum sp., Azotobacter chroococcum, Pseudomonas flourscnes, Bacillus subtilis and Bacillus pumilus in order to control Fusarium graminearum. Results of dual culture interaction test shows that the colony diameter of Fusarium graminearum was significantly reduced (P 0.05) when combined individually with Azotobacter chroococcum, Bacillus pumilus and Bacillus subtilis. However, Azospirillum sp. and Pseudomonas flourscnes comparing to the control did not affect the growth of the pathogen. Volatile metabolites produced by Azospirillum sp. affects F. graminearum negatively (P 0.05) at 3 and 6 days. The culture filtrate of Azospirillum sp. and Bacillus subtilis at three days measurement reduces the pathogen growth significantly. However, the effect of the former disappeared at 7 day while the latter continued affecting the growth of the pathogen significantly. Present study indicates acceptable levels of antagonistic activity of Azospirillum sp., Azotobacter chroococcum, Bacillus subtilis and Bacillus pumilus against Fusarium graminearum in vitro condition.

scholarly journals Eficiência de produtos biológicos na coinoculação de sementes de soja

2021 ◽  
Vol 2 (2) ◽  
pp. e21109
Author(s):  
Luana Patrícia Pinto Korber ◽  
Ângelo Henrique Canan Korber ◽  
Luciana Grange ◽  
Celestina Alflen Klahold
Keyword(s):  
Bacillus Subtilis ◽  
Bradyrhizobium Japonicum ◽  
Bacillus Amyloliquefaciens ◽  
Azospirillum Brasilense ◽  
Bacillus Pumilus ◽  
Bacillus Methylotrophicus

O objetivo deste trabalho foi verificar o potencial uso de produtos biológicos comerciais na coinoculação com Bradyrhizobium japonicum no tratamento de semente da soja. O experimento foi realizado em papel germitest e o cultivo em vasos. Foram realizados sete tratamentos com cinco repetições, sendo: T1 – testemunha, T2 –Bradyrhizobium japonicum, T3 – B. japonicum + Azospirillum brasilense, T4 – B. japonicum + Bacillus subtilis, T5 – B. japonicum + Bacillus amyloliquefaciens, T6 – B. japonicum + Bacillus methylotrophicus e T7 – B. japonicum + Bacillus pumilus. As variáveis analisadas foram germinação (G), germinação de plântulas normais (GN), índice de velocidade de emergência (IVE), crescimento parte aérea em vaso (CAV) e papel germitest (CAG), de raíz em vaso (CRV) e papel germitest (CRG). Os dados foram submetidos a análise dos pressupostos e determinados a partir do teste de Tukey a 5% de probabilidade e realização da análise de componentes principais (ACP). A coinoculação de B. japonicum + A. brasilense promoveu ganhos de enraizamento e germinação em plântulas de soja. A coinoculação com B. pumilus apresentou potencial de efeito semelhante a associação de B. japonicum + A. brasilense. A associação com B. methylotrophicus apresentou melhor enraizamento em vaso (CRV) que os demais tratamentos. O uso de BPCP’s em associação com B. japonicum potencializa o desenvolvimento inicial da plântula.

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