scholarly journals Biofilm and Cell Adhesion Strength on Dental Implant Surfaces via the Laser Spallation Technique

2019 ◽  
Author(s):  
J. D. Boyd ◽  
C.S. Miller ◽  
M. E. Grady
Keyword(s):  
Cell Adhesion ◽  
Dental Implant ◽  
Adhesion Strength ◽  
Laser Fluence ◽  
Cell Monolayer ◽  
Bacterial Biofilm ◽  
List Type ◽  
Laser Spallation ◽  
Titanium Substrates ◽  
Adhesion Index

AbstractObjectivesThe aim of this study is to quantify the adhesion strength differential between an oral bacterial biofilm and an osteoblast-like cell monolayer to a dental implant-simulant surface and develop a metric that quantifies the biocompatible efficacy of implant surfaces.MethodsHigh-amplitude short-duration stress waves generated by laser pulse absorption are used to spall bacteria and cells from titanium substrates. By carefully controlling laser fluence and calibration of laser fluence with applied stress, the adhesion difference between dental carry Streptococcus mutans biofilms and MG 63 osteoblast-like cell monolayers on smooth and rough titanium substrates is obtained. The Adhesion Index consists of a ratio of cell adhesion strength to biofilm adhesion strength obtaining a nondimensionalized parameter for biocompatibility assessments.ResultsAdhesion strength of 145±42 MPa is measured for MG 63 on smooth titanium, which increases to 288±24 MPa on roughened titanium. Adhesion strength for S. mutans on smooth titanium is 315±9 MPa and remained relatively constant at 332±9 MPa on roughened titanium. The Adhesion Index for smooth titanium is 0.46±0.12 which increased to 0.87±0.05 on roughened titanium.SignificanceThe laser spallation technique provides a platform to examine the tradeoffs of adhesion modulators on both biofilm and cell adhesion. This tradeoff is characterized by the Adhesion Index, which is proposed to aid biocompatibility screening and could result in improved implantation outcomes. The Adhesion Index is implemented to determine surface factors that promote favorable adhesion of cells greater than biofilms. Here, an Adhesion Index >> 1 suggests favorable biocompatibility.Graphical AbstractHighlightsBiofilm and cell monolayer adhesion are measured via the laser spallation techniqueSmooth and roughened dental implant-mimicking titanium surfaces are investigatedSurface roughness increases cell adhesion but does not alter the adhesion of biofilmsAn Adhesion Index is developed to directly quantify the adhesive competition between bacteria and cells on an implant surface

scholarly journals Biofilm and cell adhesion strength on dental implant surfaces via the laser spallation technique

2021 ◽  
Vol 37 (1) ◽  
pp. 48-59 ◽  
Author(s):  
J.D. Boyd ◽  
A.J. Stromberg ◽  
C.S. Miller ◽  
M.E. Grady
Keyword(s):  
Cell Adhesion ◽  
Dental Implant ◽  
Adhesion Strength ◽  
Laser Spallation

Evaluation of laser spallation as a technique for measurement of cell adhesion strength

2007 ◽  
Vol 82A (4) ◽  
pp. 852-860 ◽  
Author(s):  
Elizabeth Hagerman ◽  
Jaewoo Shim ◽  
Vijay Gupta ◽  
Ben Wu
Keyword(s):  
Cell Adhesion ◽  
Adhesion Strength ◽  
Laser Spallation

Adhesion strength measurement of polymer dielectric interfaces using laser spallation technique

2008 ◽  
Vol 516 (21) ◽  
pp. 7627-7635 ◽  
Author(s):  
Soma Sekhar V. Kandula ◽  
Cheryl D. Hartfield ◽  
Philippe H. Geubelle ◽  
Nancy R. Sottos
Keyword(s):  
Adhesion Strength ◽  
Strength Measurement ◽  
Laser Spallation ◽  
Polymer Dielectric ◽  
Dielectric Interfaces

scholarly journals Adhesion Strength of BNT Films Hydrothermally Deposited on Titanium Substrates

2010 ◽  
Vol 123-125 ◽  
pp. 399-402
Author(s):  
Fang Chao Xu ◽  
Kazuhiro Kusukawa
Keyword(s):  
Finite Element Analysis ◽  
Finite Element ◽  
Adhesion Strength ◽  
Pure Titanium ◽  
Tensile Tests ◽  
Film Deposition ◽  
Lead Free ◽  
Element Analysis ◽  
Substrate Strain ◽  
Titanium Substrates

Lead-free piezoelectric (Bi1/2Na1/2)TiO3 (BNT) films were deposited on 1 mm thick pure titanium(Ti) substrates by a hydrothermal method. Tensile tests were performed to quantitatively assess the adhesion strength between BNT films and Ti substrates. Ti substrates were pretreated by chemical polish and mechanical polish respectively prior to BNT film deposition. In the tensile test, the behavior of BNT film exfoliation was investigated by the replica method. The critical Ti substrate strain inducing BNT film exfoliation was determined by the aid of finite element analysis (FEM). In this study, the results revealed that BNT film exfoliations were caused by the strain of Ti substrate, and the mechanical polish pretreatment improved the adhesion of BNT film to Ti substrate.

scholarly journals Role of collagenase in mediating in vitro alveolar epithelial wound repair

1999 ◽  
Vol 112 (2) ◽  
pp. 243-252
Author(s):  
E. Planus ◽  
S. Galiacy ◽  
M. Matthay ◽  
V. Laurent ◽  
J. Gavrilovic ◽  
...  
Keyword(s):  
Cell Migration ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Type I Collagen ◽  
Alveolar Epithelial Cell ◽  
Cell Monolayer ◽  
Type I ◽  
Type Ii ◽  
Alveolar Epithelial

Type II pneumocytes are essential for repair of the injured alveolar epithelium. The effect of two MMP collagenases, MMP-1 and MMP-13 on alveolar epithelial repair was studied in vitro. The A549 alveolar epithelial cell line and primary rat alveolar epithelial cell cultures were used. Cell adhesion and cell migration were measured with and without exogenous MMP-1. Wound healing of a cell monolayer of rat alveolar epithelial cell after a mechanical injury was evaluated by time lapse video analysis. Cell adhesion on type I collagen, as well as cytoskeleton stiffness, was decreased in the presence of exogenous collagenases. A similar decrease was observed when cell adhesion was tested on collagen that was first incubated with MMP-1 (versus control on intact collagen). Cell migration on type I collagen was promoted by collagenases. Wound healing of an alveolar epithelial cell monolayer was enhanced in the presence of exogenous collagenases. Our results suggest that collagenases could modulate the repair process by decreasing cell adhesion and cell stiffness, and by increasing cell migration on type I collagen. Collagen degradation could modify cell adhesion sites and collagen degradation peptides could induce alveolar type II pneumocyte migration. New insights regarding alveolar epithelial cell migration are particularly relevant to investigate early events during alveolar epithelial repair following lung injury.

Hydrodynamically-Confined Microflows for Cell Adhesion Strength Measurement

2010 ◽  
Author(s):  
Kevin V. Christ ◽  
Kevin T. Turner
Keyword(s):  
Cell Adhesion ◽  
Adhesion Strength ◽  
Cell Biology ◽  
Microfluidic Devices ◽  
Cell Behavior ◽  
Shear Stresses ◽  
Strength Measurement ◽  
Coated Surfaces ◽  
Biology Research ◽  
Standard Techniques

Cell adhesion plays a fundamental role in numerous physiological and pathological processes, and measurements of the adhesion strength are important in fields ranging from basic cell biology research to the development of implantable biomaterials. Our group and others have recently demonstrated that microfluidic devices offer advantages for characterizing the adhesion of cells to protein-coated surfaces [1,2]. Microfluidic devices offer many advantages over conventional assays, including the ability to apply high shear stresses in the laminar regime and the opportunity to directly observe cell behavior during testing. However, a key disadvantage is that such assays require cells to be cultured inside closed microchannels. Assays based on closed channels restrict the types of surfaces that can be examined and are not compatible with many standard techniques in cell biology research. Furthermore, while techniques for cell culture in microchannels have become common, maintaining the viability of certain types of cells in channels remains a challenge.

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