Hydrodynamically-Confined Microflows for Cell Adhesion Strength Measurement

2010 ◽  
Author(s):  
Kevin V. Christ ◽  
Kevin T. Turner
Keyword(s):  
Cell Adhesion ◽  
Adhesion Strength ◽  
Cell Biology ◽  
Microfluidic Devices ◽  
Cell Behavior ◽  
Shear Stresses ◽  
Strength Measurement ◽  
Coated Surfaces ◽  
Biology Research ◽  
Standard Techniques

Cell adhesion plays a fundamental role in numerous physiological and pathological processes, and measurements of the adhesion strength are important in fields ranging from basic cell biology research to the development of implantable biomaterials. Our group and others have recently demonstrated that microfluidic devices offer advantages for characterizing the adhesion of cells to protein-coated surfaces [1,2]. Microfluidic devices offer many advantages over conventional assays, including the ability to apply high shear stresses in the laminar regime and the opportunity to directly observe cell behavior during testing. However, a key disadvantage is that such assays require cells to be cultured inside closed microchannels. Assays based on closed channels restrict the types of surfaces that can be examined and are not compatible with many standard techniques in cell biology research. Furthermore, while techniques for cell culture in microchannels have become common, maintaining the viability of certain types of cells in channels remains a challenge.

scholarly journals Eukaryotic systems broaden the scope of synthetic biology

2009 ◽  
Vol 187 (5) ◽  
pp. 589-596 ◽  
Author(s):  
Karmella A. Haynes ◽  
Pamela A. Silver
Keyword(s):  
Synthetic Biology ◽  
Nucleic Acids ◽  
Cell Biology ◽  
Cell Behavior ◽  
Cellular Functions ◽  
Complex Cells ◽  
Cellular Processes ◽  
Challenges And Opportunities ◽  
Foundational Work ◽  
Biology Research

Synthetic biology aims to engineer novel cellular functions by assembling well-characterized molecular parts (i.e., nucleic acids and proteins) into biological “devices” that exhibit predictable behavior. Recently, efforts in eukaryotic synthetic biology have sprung from foundational work in bacteria. Designing synthetic circuits to operate reliably in the context of differentiating and morphologically complex cells presents unique challenges and opportunities for progress in the field. This review surveys recent advances in eukaryotic synthetic biology and describes how synthetic systems can be linked to natural cellular processes in order to manipulate cell behavior and to foster new discoveries in cell biology research.

Optical Manipulation of Inorganic and Organic Objects in Soft Microfluidic Devices

2000 ◽  
Vol 657 ◽  
Author(s):  
Cengiz S. Ozkan ◽  
Erhan Ata ◽  
Mihrimah Ozkan ◽  
Sadik C. Esener
Keyword(s):  
Optical Tweezers ◽  
Cell Sorting ◽  
Cell Biology ◽  
Microfluidic Devices ◽  
Optical Manipulation ◽  
Microfluidic Channels ◽  
Negative Photoresist ◽  
Pdms Elastomer ◽  
Biology Research ◽  
Inorganic And Organic

ABSTRACTWe describe a technique for trapping and manipulation of inorganic and organic objects in microfluidic channels, based on photonic momentum transfer using an optical tweezers arrangement. Microfluidic devices have been fabricated by polydimethylsiloxane (PDMS) elastomer molding of patterns lithographically defined on a thick negative photoresist. Polystyrene microspheres dispersed in water were transferred into the fluidic channels using a syringe pump. Microspheres and live biological cells are trapped and redirected by optical manipulation within the fluidic channels. Optical trapping and patterning will have applications in creation of active cellular arrays for cell biology research, tissue engineering, cell sorting and drug discovery.

Well Plate—Coupled Microfluidic Devices Designed for Facile Image-Based Cell Adhesion and Transmigration Assays

2009 ◽  
Vol 15 (1) ◽  
pp. 102-106 ◽  
Author(s):  
Carolyn G. Conant ◽  
Michael A. Schwartz ◽  
Cristian Ionescu-Zanetti
Keyword(s):  
Cell Adhesion ◽  
Shear Flow ◽  
Cell Biology ◽  
Mononuclear Cells ◽  
Biological Significance ◽  
Microfluidic Devices ◽  
Response Curves ◽  
Single Experiment ◽  
Cell Monolayers ◽  
Biological Phenomena

Microfluidic devices have become invaluable tools in recent years to model biological phenomena. Here, the authors present a well plate microfluidic (WPM) device for conducting cell biology assays under shear flow. Physiological shear flow conditions of cell-cell and cell-ligand adhesion within this device produce results with higher biological significance than conventional well plates. The WPM format also produced significant work flow advantages such as faster liquid handling compared to static well plate assays. The authors used the VLA-4—VCAM-1 cell adhesion model as the basis for a rapid, higher throughput adhesion inhibition screen of monoclonal antibodies against VLA-4. Using the WPM device, they generated IC50 dose-response curves 96 times faster than conventional flow cells. The WPM device was also used to study transmigration of mononuclear cells through endothelial cell monolayers. Twenty-four channels of transmigration data were generated in a single experiment.

scholarly journals Surface modification of PDMS-based microfluidic devices with collagen using polydopamine as a spacer to enhance primary human bronchial epithelial cell adhesion

2020 ◽  
Author(s):  
Mohammadhossein Dabaghi ◽  
Shadi Shahriari ◽  
Neda Saraei ◽  
Kevin Da ◽  
Abiram Chandiramohan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Surface Modification ◽  
Cell Adhesion ◽  
Microfluidic Devices ◽  
Extracellular Matrix Protein ◽  
Shear Stresses ◽  
Bronchial Epithelial ◽  
Ecm Proteins ◽  
Coating Methods ◽  
Organ On A Chip

AbstractPolydimethylsiloxane (PDMS) is a silicone-based synthetic material that is used in various biomedical applications due to its properties, including transparency, flexibility, permeability to gases, and ease of use. Though PDMS facilitates and realizes the fabrication of complicated geometries at the micro and nano scales, it does not optimally interact with cells for adherence and proliferation. Different strategies have been proposed to render PDMS to enhance cell attachment. The majority of these surface modification techniques have been offered for a static cell culture system. However, dynamic cell culture systems such as organ-on-a-chip devices are demanding platforms that recapitulate the complexity of a living tissue microenvironment. For organ-on-a-chip platforms, PDMS surfaces are usually coated by ECM proteins, which occur as a result of physical, weak bonding between PDMS and ECM proteins, and this binding can be degraded when it is exposed to shear stresses. This work reports static and dynamic coating methods to covalently bind collagen within a PDMS-based microfluidic device using polydopamine (PDA). These coating methods were evaluated using water contact angle measurement and atomic force microscopy (AFM) to find the optimum coating conditions. The biocompatibility of collagen-coated PDMS devices was assessed by culturing primary human bronchial epithelial cells (HBECs) in microfluidic devices. It was shown that both PDA coating methods could be used to bind collagen, thereby improving cell adhesion (around three times higher) without showing any discernible difference. These results suggested that such a surface modification can be used to coat an extracellular matrix protein onto PDMS-based microfluidic devices.

scholarly journals Mammalian Cell Behavior on Hydrophobic Substrates: Influence of Surface Properties

2019 ◽  
Vol 3 (2) ◽  
pp. 48 ◽  
Author(s):  
Michele Ferrari ◽  
Francesca Cirisano ◽  
M. Carmen Morán
Keyword(s):  
Cell Adhesion ◽  
Surface Chemistry ◽  
Cell Viability ◽  
Surface Charge ◽  
Surface Properties ◽  
Mammalian Cell ◽  
Cell Biology ◽  
Biomedical Applications ◽  
Cell Behavior ◽  
Water Repellent

The influence of different surface properties holding to a modification of the substrate towards hydrophobic or superhydrophobic behavior was reviewed in this paper. Cell adhesion, their communication, and proliferation can be strongly manipulated, acting on interfacial relationship involving stiffness, surface charge, surface chemistry, roughness, or wettability. All these features can play mutual roles in determining the final properties of biomedical applications ranging from fabrics to cell biology devices. The focus of this work is the mammalian cell viability in contact with moderate to highly water repellent coatings or materials and also in combination with hydrophilic areas for more specific application. Few case studies illustrate a range of examples in which these surface properties and design can be fruitfully matched to the specific aim.

Recent developments in microfluidic devices for in vitro cell culture for cell-biology research

2012 ◽  
Vol 35 ◽  
pp. 150-164 ◽  
Author(s):  
Dan Gao ◽  
Hongxia Liu ◽  
Yuyang Jiang ◽  
Jin-Ming Lin ◽  
Dan Gao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Biology ◽  
Microfluidic Devices ◽  
In Vitro Cell Culture ◽  
Recent Developments ◽  
Biology Research

Adhesion strength measurement of polymer dielectric interfaces using laser spallation technique

2008 ◽  
Vol 516 (21) ◽  
pp. 7627-7635 ◽  
Author(s):  
Soma Sekhar V. Kandula ◽  
Cheryl D. Hartfield ◽  
Philippe H. Geubelle ◽  
Nancy R. Sottos
Keyword(s):  
Adhesion Strength ◽  
Strength Measurement ◽  
Laser Spallation ◽  
Polymer Dielectric ◽  
Dielectric Interfaces

Use of cultured epithelia to study transport and its regulation

1983 ◽  
Vol 106 (1) ◽  
pp. 55-69
Author(s):  
J. S. Handler
Keyword(s):  
Cell Biology ◽  
Culture Conditions ◽  
Millipore Filter ◽  
A6 Epithelia ◽  
Amphibian Urinary Bladder ◽  
Polarized Epithelia ◽  
Cultured Epithelia ◽  
Camp Levels ◽  
Epithelia Transport ◽  
Standard Techniques

Epithelial cells from a variety of species and organs form polarized epithelia in culture. When epithelia are grown on a porous surface, such as a millipore filter, transport can be studied using adaptations of standard techniques. In the few years in which cultured epithelia have been studied by transport physiologists, most work has been focused on identification and description of the differentiated transport exhibited by cultured epithelia. Epithelia formed by a continuous line of cells derived from pig kidney (LLC-PK1) exhibit sodium-coupled glucose transport similar to that of the proximal tubule and have vasopressin-sensitive adenylate cyclase that has been studied in great detail. Also of interest are epithelia formed by continuous lines of cells derived from amphibian kidney (A6) and from amphibian urinary bladder (TBM). Each line forms epithelia that have high electrical resistance and amiloride-sensitive sodium transport. Transport is stimulated by aldosterone and by cAMP or hormones that raise cell cAMP levels. In LLC-PK1 and in A6 epithelia, transport and the response to hormones can be manipulated by manipulating the culture conditions. Cultured epithelia have also been used to explore the cell biology of epithelia. Most interesting in this regard are studies of the development and maintenance of epithelial cell polarity. This approach should be especially valuable.

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