scholarly journals Soybean CHX protein GmSALT3 confers leaf Na+ exclusion via a root derived mechanism, and Cl− exclusion via a shoot derived process

2020 ◽  
Author(s):  
Yue Qu ◽  
Rongxia Guan ◽  
Jayakumar Bose ◽  
Sam W. Henderson ◽  
Stefanie Wege ◽  
...  
Keyword(s):  
Glycine Max ◽  
Salt Tolerance ◽  
Time Course ◽  
Near Isogenic Lines ◽  
Salt Tolerant ◽  
In Planta ◽  
K Uptake ◽  
Phloem Sap ◽  
E Coli ◽  
Salt Exclusion

AbstractSoybean (Glycine max) yields are threatened by multiple stresses including soil salinity. GmSALT3 confers net shoot exclusion for both Na+ and Cl− and improves salt tolerance of soybean; however, how the ER-localised GmSALT3 achieves this is unknown. Here, GmSALT3’s function was investigated in heterologous systems and near-isogenic lines that contained the full-length GmSALT3 (NIL-T; salt-tolerant) or a truncated transcript Gmsalt3 (NIL-S; salt-sensitive). GmSALT3 restored growth of K+-uptake-defective E. coli and contributed toward net influx and accumulation of Na+, K+, and Cl− in Xenopus laevis oocytes, while Gmsalt3 was non-functional. A time-course analysis of the NILs confirmed that shoot Cl− exclusion breaks down prior to Na+ exclusion, while grafting showed that shoot Na+ exclusion occurs via a root xylem-based mechanism. In contrast, NIL-T plants exhibited significantly greater Cl− content in both the stem xylem and phloem sap compared to NIL-S, indicating that shoot Cl− exclusion likely depends upon novel phloem-based Cl− recirculation. NIL-T shoots grafted on NIL-S roots contained low shoot Cl−, which confirmed that Cl− recirculation is dependent on the presence of GmSALT3 in shoots. Overall, these findings provide new insights on GmSALT3’s impact on salinity tolerance and reveal a novel mechanism for shoot Cl– exclusion in plants.HighlightGmSALT3 improves soybean salt tolerance. Here, using heterologous expression, we found GmSALT3 is a functional ion transporter, and, in planta that it confers shoot salt exclusion through root-based Na+ xylem exclusion and shoot-based Cl− exclusion via phloem derived Cl- recirculation.

scholarly journals Identification of novel source of salt tolerance in local bread wheat germplasm using morpho-physiological and biochemical attributes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nadeem Hussain ◽  
Abdul Ghaffar ◽  
Zafar Ullah Zafar ◽  
Muhammad Javed ◽  
Kausar Hussain Shah ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Photosynthetic Activity ◽  
Wheat Breeding ◽  
Molecular Techniques ◽  
Growth And Yield ◽  
Wheat Cultivars ◽  
Salt Tolerant ◽  
Wheat Germplasm ◽  
Salt Exclusion

AbstractSalt tolerant wheat cultivars may be used as genetic resource for wheat breeding to ensure yield stability in future. The study was aimed to select salt tolerant cultivar(s) to identify novel source of salt tolerance in local wheat germplasm. Initially, 40 local wheat cultivars were screened at 150 mM NaCl stress at seedling stage. Selected salt-tolerant (three; S-24, LU-26S and Pasban-90) and salt-sensitive (four; MH-97, Kohistan-97, Inqilab-91 and Iqbal-2000) wheat cultivars were further evaluated using growth, yield, biochemical and physiological attributes. Growth and yield of selected cultivars were reduced under salt stress due to decline in plant water status, limited uptake of macronutrients (N, P and K), reduced K+/Na+ ratio, photosynthetic pigments and quantum yield of PSII. Wheat plants tried to acclimate salt stress by osmotic adjustment (accumulation of total soluble sugars, proline and free amino acids). Degree of salinity tolerance in cvs. S-24 and LU-26S found to be associated with maintenance of K+/Na+ ratio, osmo-protectant and photosynthetic activity and can be used as donor for salt tolerance in wheat breeding program at least in Pakistan. These cultivars can be further characterized using molecular techniques to identify QTLs/genes for salt exclusion, osmo-protectant and photosynthetic activity for molecular breeding.

scholarly journals Hybrid identification for Glycine max and Glycine soja with SSR markers and analysis of salt tolerance

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6483 ◽  
Author(s):  
Fayuan Li ◽  
Xun Liu ◽  
Shengyan Wu ◽  
Qingyun Luo ◽  
Bingjun Yu
Keyword(s):  
Glycine Max ◽  
Salt Tolerance ◽  
Ssr Markers ◽  
Glycine Soja ◽  
Female Parent ◽  
Tolerance Test ◽  
Dry Matter Accumulation ◽  
Salt Tolerant ◽  
Cross Combination ◽  
Hybrid Identification

Glycine max cultivars Lee68, Nannong 1138-2, and Nannong 8831 were used as the female parents, and hybrid lines (F5) 4,111, 4,076 (N23674 × BB52), 3,060 (Lee68 × N23227), and 185 (Jackson × BB52) that selected for salt tolerance generation by generation from the cross combination of G. max and G. soja were used as the male parents, 11 (A–K) backcrosses or three-way crosses were designed and 213 single hybrids were harvested. The optimized soybean simple sequence repeat (SSR)–polymerase chain reaction (PCR) system was used to analyze the SSR polymorphism of above parental lines and get the parental co-dominant SSR markers for hybrid identification, and in which 30 true hybrids were gained. The true hybrids (G1, G3, G9, G12, G13, G16) of G cross combination were chosen as the representative for the salt tolerance test, and the results showed that, as exposed to salt stress, the seedlings of G9 line displayed higher salt tolerant coefficient, relative growth rate, and dry matter accumulation, when compared with their female parent Nannong 1138-2, and even performed equally strong salt tolerance as the male parent 3,060. It provides a feasible method of the combination of molecular SSR markers and simple physiological parameters to identify the true hybrids of G. max and G. soja, and to innovate the salt-tolerant soybean germplasms.

Application of SSR Technique for the Identification of Markers Linked to Salinity Tolerance in Rice

2013 ◽  
Vol 19 (2) ◽  
pp. 57-65
Author(s):  
MH Kabir ◽  
MM Islam ◽  
SN Begum ◽  
AC Manidas
Keyword(s):  
Salt Tolerance ◽  
Ssr Markers ◽  
Salinity Tolerance ◽  
Seedling Stage ◽  
Phenotypic Screening ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Hydroponic System ◽  
Rice Landrace ◽  
Salt Susceptible

A cross was made between high yielding salt susceptible BINA variety (Binadhan-5) with salt tolerant rice landrace (Harkuch) to identify salt tolerant rice lines. Thirty six F3 rice lines of Binadhan-5 x Harkuch were tested for salinity tolerance at the seedling stage in hydroponic system using nutrient solution. In F3 population, six lines were found as salt tolerant and 10 lines were moderately tolerant based on phenotypic screening at the seedling stage. Twelve SSR markers were used for parental survey and among them three polymorphic SSR markers viz., OSR34, RM443 and RM169 were selected to evaluate 26 F3 rice lines for salt tolerance. With respect to marker OSR34, 15 lines were identified as salt tolerant, 9 lines were susceptible and 2 lines were heterozygous. While RM443 identified 3 tolerant, 14 susceptible and 9 heterozygous rice lines. Eight tolerant, 11 susceptible and 7 heterozygous lines were identified with the marker RM169. Thus the tested markers could be efficiently used for tagging salt tolerant genes in marker-assisted breeding programme.DOI: http://dx.doi.org/10.3329/pa.v19i2.16929 Progress. Agric. 19(2): 57 - 65, 2008

Genetic Potential for Salt Tolerance During Germination in Lycopersicon Species

HortScience ◽  
1997 ◽  
Vol 32 (2) ◽  
pp. 296-300 ◽  
Author(s):  
M.R. Foolad ◽  
G.Y. Lin
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Genotypic Variation ◽  
Control Treatment ◽  
Molar Ratio ◽  
Wild Accession ◽  
Salt Tolerant ◽  
Plant Introductions ◽  
Genetic Potential ◽  
Stress Levels

Seed of 42 wild accessions (Plant Introductions) of Lycopersicon pimpinellifolium Jusl., 11 cultigens (cultivated accessions) of L. esculentum Mill., and three control genotypes [LA716 (a salt-tolerant wild accession of L. pennellii Corr.), PI 174263 (a salt-tolerant cultigen), and UCT5 (a salt-sensitive breeding line)] were evaluated for germination in either 0 mm (control) or 100 mm synthetic sea salt (SSS, Na+/Ca2+ molar ratio equal to 5). Germination time increased in response to salt-stress in all genotypes, however, genotypic variation was observed. One accession of L. pimpinellifolium, LA1578, germinated as rapidly as LA716, and both germinated more rapidly than any other genotype under salt-stress. Ten accessions of L. pimpinellifolium germinated more rapidly than PI 174263 and 35 accessions germinated more rapidly than UCT5 under salt-stress. The results indicate a strong genetic potential for salt tolerance during germination within L. pimpinellifolium. Across genotypes, germination under salt-stress was positively correlated (r = 0.62, P < 0.01) with germination in the control treatment. The stability of germination response at diverse salt-stress levels was determined by evaluating germination of a subset of wild, cultivated accessions and the three control genotypes at 75, 150, and 200 mm SSS. Seeds that germinated rapidly at 75 mm also germinated rapidly at 150 mm salt. A strong correlation (r = 0.90, P < 0.01) existed between the speed of germination at these two salt-stress levels. At 200 mm salt, most accessions (76%) did not reach 50% germination by 38 days, demonstrating limited genetic potential within Lycopersicon for salt tolerance during germination at this high salinity.

scholarly journals Evaluation of salinity tolerance indices in North African barley accessions at reproductive stage

2019 ◽  
Vol 55 (No. 2) ◽  
pp. 61-69 ◽  
Author(s):  
Dorsaf Allel ◽  
Anis BenAmar ◽  
Mounawer Badri ◽  
Chedly Abdelly
Keyword(s):  
Salt Tolerance ◽  
Grain Yield ◽  
Salinity Tolerance ◽  
Selection Criteria ◽  
Reproductive Stage ◽  
Shoot Biomass ◽  
Salt Tolerant ◽  
North African ◽  
Grain Number ◽  
Selection Indices

Soil salinity is one of the main factors limiting cereal productivity in worldwide agriculture. Exploitation of natural variation in local barley germplasm is an effective approach to overcome yield losses. Three gene pools of North African Hordeum vulgare L. grown in Tunisia, Algeria and Egypt were evaluated at the reproductive stage under control and saline conditions. Assessment of stress tolerance was monitored using morphological, yield-related traits and phenological parameters of reproductive organs showing significant genetic variation. High heritability and positive relationships were found suggesting that some traits associated with salt tolerance could be used as selection criteria. The phenotypic correlations revealed that vegetative traits including shoot biomass, tiller number and leaf number along with yield-related traits such as spike number, one spike dry weight, grain number/plant and grain number/spike were highly positively correlated with grain yield under saline conditions. Hence, these traits can be used as reliable selection criteria to improve barley grain yield. Keeping a higher shoot biomass and longer heading and maturity periods as well as privileged filling ability might contribute to higher grain production in barley and thus could be potential target traits in barley crop breeding toward improvement of salinity tolerance. Multiple selection indices revealed that salt tolerance trait index provided a better discrimination of barley landraces allowing selection of highly salt-tolerant and highly productive genotypes under severe salinity level. Effective evaluation of salt tolerance requires an integration of selection indices to successfully identify and characterize salt tolerant lines required for valuable exploitation in the management of salt-affected areas.  

scholarly journals Early Growth Stage Characterization and the Biochemical Responses for Salinity Stress in Tomato

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 712
Author(s):  
Md Sarowar Alam ◽  
Mark Tester ◽  
Gabriele Fiene ◽  
Magdi Ali Ahmed Mousa
Keyword(s):  
Salt Tolerance ◽  
Salinity Tolerance ◽  
Salinity Treatment ◽  
Salt Tolerant ◽  
Improvement Program ◽  
Biochemical Basis ◽  
Elevated Salinity ◽  
Tolerance Indices ◽  
Promising Source ◽  
Tomato Genotypes

Salinity is one of the most significant environmental stresses for sustainable crop production in major arable lands of the globe. Thus, we conducted experiments with 27 tomato genotypes to screen for salinity tolerance at seedling stage, which were treated with non-salinized (S1) control (18.2 mM NaCl) and salinized (S2) (200 mM NaCl) irrigation water. In all genotypes, the elevated salinity treatment contributed to a major depression in morphological and physiological characteristics; however, a smaller decrease was found in certain tolerant genotypes. Principal component analyses (PCA) and clustering with percentage reduction in growth parameters and different salt tolerance indices classified the tomato accessions into five key clusters. In particular, the tolerant genotypes were assembled into one cluster. The growth and tolerance indices PCA also showed the order of salt-tolerance of the studied genotypes, where Saniora was the most tolerant genotype and P.Guyu was the most susceptible genotype. To investigate the possible biochemical basis for salt stress tolerance, we further characterized six tomato genotypes with varying levels of salinity tolerance. A higher increase in proline content, and antioxidants activities were observed for the salt-tolerant genotypes in comparison to the susceptible genotypes. Salt-tolerant genotypes identified in this work herald a promising source in the tomato improvement program or for grafting as scions with improved salinity tolerance in tomato.

Short-term salt tolerance mechanisms in differentially salt tolerant tomato species

1999 ◽  
Vol 37 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Ana Santa-Cruz ◽  
Manuel Acosta ◽  
Ana Rus ◽  
Maria C. Bolarin
Keyword(s):  
Salt Tolerance ◽  
Salt Tolerant ◽  
Tolerance Mechanisms ◽  
Short Term ◽  
Tomato Species

Differences in solubility parameters and susceptibility to salting-out between softwood and hardwood lignosulfonates

Holzforschung ◽  
2016 ◽  
Vol 70 (11) ◽  
pp. 1015-1021 ◽  
Author(s):  
Bernt O. Myrvold
Keyword(s):  
Molecular Weight ◽  
Salt Tolerance ◽  
Plant Species ◽  
Post Treatment ◽  
Solubility Parameters ◽  
Minor Effect ◽  
Hofmeister Series ◽  
Salt Tolerant ◽  
Salting Out ◽  
A Minor

Abstract The salting-out of lignosulfonates (LSs) follows the Hofmeister series, but different LSs show different susceptibility to salting-out. By comparing results for a large number of LSs from different plant species, pulping conditions, and post-treatment of the LS, it was found that the salting-out concentration for different LSs is mainly due to differences in the plant species with guaiacyl LS being much more salt-tolerant than guaiacyl-syringyl LS. The pulping conditions have only a minor effect on the salt tolerance of the different LSs. Post-treatment that increases the molecular weight or reduces the sulfonation also reduces the salt tolerance.

scholarly journals Evolution of halophytes: multiple origins of salt tolerance in land plants

2010 ◽  
Vol 37 (7) ◽  
pp. 604 ◽  
Author(s):  
Timothy J. Flowers ◽  
Hanaa K. Galal ◽  
Lindell Bromham
Keyword(s):  
Salt Tolerance ◽  
Novel Species ◽  
Complex Trait ◽  
Land Plants ◽  
Salt Tolerant ◽  
Physiological Mechanisms ◽  
Multiple Origins ◽  
Naturally Occurring ◽  
Tolerant Plants

The evolution of salt tolerance is interesting for several reasons. First, since salt-tolerant plants (halophytes) employ several different mechanisms to deal with salt, the evolution of salt tolerance represents a fascinating case study in the evolution of a complex trait. Second, the diversity of mechanisms employed by halophytes, based on processes common to all plants, sheds light on the way that a plant’s physiology can become adapted to deal with extreme conditions. Third, as the amount of salt-affected land increases around the globe, understanding the origins of the diversity of halophytes should provide a basis for the use of novel species in bioremediation and conservation. In this review we pose the question, how many times has salt tolerance evolved since the emergence of the land plants some 450–470 million years ago? We summarise the physiological mechanisms underlying salt-tolerance and provide an overview of the number and diversity of salt-tolerant terrestrial angiosperms (defined as plants that survive to complete their life cycle in at least 200 mM salt). We consider the evolution of halophytes using information from fossils and phylogenies. Finally, we discuss the potential for halophytes to contribute to agriculture and land management and ask why, when there are naturally occurring halophytes, it is proving to be difficult to breed salt-tolerant crops.

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