An amended potassium persulfate ABTS antioxidant assay used for medicinal plant extracts revealed variable antioxidant capacity based upon plant extraction process

2020 ◽  
Author(s):  
Cody E. Mingle ◽  
Anthony L. Newsome
Keyword(s):  
Antioxidant Activity ◽  
Plant Extracts ◽  
Biological Fluids ◽  
Antioxidant Properties ◽  
Cation Radical ◽  
Microtiter Plate ◽  
Potassium Persulfate ◽  
Antioxidant Assay ◽  
Medicinal Plant Extracts ◽  
Microtiter Plate Format

AbstractUse of potassium persulfate (K2S208) for oxidation of 7.0 mM ABTS to a stable ABTS radical for antioxidant studies was first reported in 1999. A feature of this popular antioxidant assay has been the requirement of an overnight reaction (6 to 12 h) for the formation of a stable ABTS colored radical. It is now reported that when the concentration of ABTS is lowered to 0.7 mM, complete oxidation to the stable cation radical occurs in 30 min, thus circumventing the necessary overnight step. Using this format, it is now possible to accurately assess antioxidant activity based on the potassium persulfate/ABTS format in less than one hour which includes formation time of a stable ABTS radical. This methodology documented the presence of antioxidant properties of plant extracts used in Traditional Chinese Medicine. The degree of antioxidant activity was directly related to the extraction method. Greater antioxidant activity was associated with butanol extraction. When incorporated into a microtiter plate format, it supported rapid assessment of multiple determinations of dilutions of plant extracts in less than one hour which included time required for formation of a stable ABTS radical. The ease, improved time prerequisites, and minimal reagent needs with the microtiter plate format, makes this design attractive. It would prove of particular interest to individuals engaged in both smaller and high-volume throughput antioxidant assays of food and health products, and other biological fluids and tissues.

scholarly journals Acetylcholinesterase Inhibitory Activity and Antioxidant Activity of Selected Medicinal Plant Extracts Used Against Cognitive Dysfunction and Memory Loss in Sri Lankan Traditional Medicine

2018 ◽  
Vol 6 (5) ◽  
pp. 16-25
Author(s):  
Anoja Priyadarshani Attanayake ◽  
Kamani Ayoma Perera Wijewardana Jayatilaka
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Plant Extracts ◽  
Inhibitory Activity ◽  
Ricinus Communis ◽  
Centella Asiatica ◽  
Sri Lankan ◽  
Medicinal Plant Extracts ◽  
Ache Inhibitory Activity ◽  
Alzheimer’S Diseases

The objectives of the study were to determine the acetylcholinesterase (AChE) inhibitory activity and antioxidant activity in the selected Sri Lankan medicinal plants. AChE inhibitory activity of the selected medicinal plant extracts was determined using the Ellman’s method. The antioxidant activities were determined by four in vitro methods namely DPPH assay, FRAP assay, NO assay and TBA acid method.  The contents of total polyphenols and flavonoids were determined quantitatively.Out of the ten selected medicinal plant extracts Abrus precatorius, Centella asiatica, Strychnos nux-vomica and Ricinus communis showed IC50 values < 200 µg/mL for AChE inhibitory activity. A high antioxidant activity was shown in the extracts of Cardiosprmum halicacabum, Centella asiatica and  Ricinus communis in  the four selected antioxidant assays.  The total polyphenol content and total flavonoid content was in the range of 0.55-7.30 mg/g DW and 19.08±0.29-1283.08± 0.09 µg/gDW. The extracts of A. precatorius, C. asiatica, S. nux-vomica and R. communis are deserved to be as potent sources of AChE inhibitors as well as natural antioxidants. Considering the complex multifactorial etiology of Alzheimer's diseases, these plant extracts would be apt candidates for the development of novel pharmaceutical agents in the management of Alzheimer's diseases.  

scholarly journals Antioxidant Activity of Plant Extracts from Colombian Coffee-Growing Eco-Region

2017 ◽  
Vol 13 (1) ◽  
pp. 56-59
Author(s):  
Aura M. Blandón ◽  
Oscar M. Mosquera ◽  
Antônio E. G. Sant’ana ◽  
Aldenir F. Dos Santos ◽  
Luana L. S. Pires
Keyword(s):  
Antioxidant Activity ◽  
Plant Species ◽  
Plant Extracts ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Dpph Assay ◽  
Methanolic Extracts ◽  
In Vitro Antioxidant Activity

The present study describes the in vitro antioxidant activity of methanol extracts of 34 plant species collected in the Colombian coffee-growing eco-region belonging to Euphorbiaceae, Piperaceae and Solanaceae families. The antioxidant properties of extracts were evaluated by determining radical scavenging power measured with a DPPH assay. The methanolic extracts of  Hyeronimia antioquiensis, Mabea montana, and Alchornea grandis species (Euphorbiaceae), presents EC50 values equal to 0.686, 12.35, and 13.01 µg/mL, respectively, showing high antioxidant potential.

scholarly journals Evaluation of cellular antioxidant activity of selected species of Caralluma and Boucerosia on cell lines

2014 ◽  
Vol 2 (1) ◽  
pp. 83-87 ◽  
Author(s):  
Madhuri Vajha ◽  
Siva Rama Krishna Chillara
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Plant Extracts ◽  
Inhibitory Concentration ◽  
Antioxidant Properties ◽  
Raw 264.7 Cells ◽  
Cellular Antioxidant Activity ◽  
Methanolic Extracts ◽  
Normal Metabolism ◽  
Promising Source

Objective: The aim of the present work was designed to study cellular antioxidant properties of selected plant extracts of Caralluma and Boucerosia on cell lines. Materials and Methods: The present study was carried out in four species of Caralluma R.Br. such as Caralluma adscendens (Roxb.) R. Brown var. attenuata (Wight) Grav. & Mayur. (CAA), Caralluma adscendens (Roxb.) R. Brown var. fimbriata (Wall.) Gravely & Mayur. (CAF), Caralluma stalagmifera C.E.C. Fisch. (CS) and Caralluma stalagmifera C.E.C. Fisch. var. longipetala  Karupp. & Pull. (CSL) and as well as two species of Boucerosia Wight & Arn. such as Boucerosia lasiantha Wight. (BL) and Boucerosia umbellata (Haw.) Wight & Arn.  (BU). The antioxidant efficacy was evaluated by ability of antioxidant molecules in the extract to scavenge ROS produced by normal metabolism by cells, observed by the oxidation of 2, 7- Dichloro fluorescein diacetate to dichlorofluorescein, followed by decrease in fluorescence intensity. Results: Cellular antioxidant activity was demonstrated by the inhibitory concentration 50% (IC50) of reactive oxygen species (ROS). The percentage ROS inhibition in Raw 264.7 cells when treated with different concentrations of methanolic extracts showed variations among selected species. Conclusion: The plant extracts were shown to be effective for antioxidant potential and can be a promising source of natural antioxidants.DOI: http://dx.doi.org/10.3126/ijasbt.v2i1.9649Int J Appl Sci Biotechnol, Vol. 2(1): 83-87

scholarly journals Evaluation of Antioxidant Activity of Medicinal Plant Extracts Produced for Commercial Purpose

2011 ◽  
Vol 8 (2) ◽  
pp. 882-886 ◽  
Author(s):  
A. D. Sathisha ◽  
H. B. Lingaraju ◽  
K. Sham Prasad
Keyword(s):  
Antioxidant Activity ◽  
Plant Extracts ◽  
Curcuma Longa ◽  
Reducing Power ◽  
Tribulus Terrestris ◽  
Coffee Bean ◽  
Medicinal Plant Extracts ◽  
Commercial Purpose ◽  
Herbal Plant

The antioxidant potential of some herbal plant extracts (commercial products) was measured using variousin vitroassays. Among the extracts fromCurcuma longa, Caffea arabica, Tribulus terrestris, Bacopa monnieriandTrigonella foenum- graecum, theCurcuma longaand coffee bean extract (Caffea Arabica) showed greater antioxidant activity measured as scavenging of DPPH, superoxide radicals, reducing power and inhibition of microsomal lipid peroxidation.

scholarly journals In vitro Screening Antiviral Activity of Thai Medicinal Plants Against Porcine Reproductive and Respiratory Syndrome Virus

2020 ◽  
Author(s):  
Chaiwat Arjin ◽  
Kidsadagon Pringproa ◽  
Surat Hongsibsong ◽  
Warintorn Ruksiriwanich ◽  
Mintra Seel-audom ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Plant Extracts ◽  
Antioxidant Properties ◽  
Virus Titer ◽  
Economic Losses ◽  
Respiratory Syndrome Virus ◽  
Total Phenol Content ◽  
Medicinal Plant Extracts ◽  
Thai Medicinal Plants

Abstract Background Porcine reproductive and respiratory syndrome (PRRS) caused by the PRRS virus (PRRSV) results in economic losses in the swine industry globally. Several studies have investigated the use of plant extracts for the prevention and control of PRRS outbreaks. Thai medicinal plants may be useful for treating PRRSV infection in pigs. Therefore, we investigated the in vitro anti-PRRSV and antioxidant properties of seven Thai medicinal plants: Caesalpinia sappan Linn., Garcinia mangostana Linn., Houttuynia cordata , Perilla frutescens , Clinacanthus nutans , Phyllanthus emblica , and Tiliacora triandra .Results In the antiviral screening test, we observed that the T. triandra extract strongly inhibited the infectivity of PRRSV into MARC-145 cells [virus titer 3.5 median tissue culture infectious doses (TCID 50 )/ml (log 10 )] at 24 h post-infection, whereas the C. sappan Linn. extract strongly inhibited PRRSV replication [virus titer 2.5 TCID 50 /ml (log 10 )] at 72 h postinfection. C. sappan Linn. extract had the highest total phenol content [220.52 mM gallic acid equivalents (GAE)/g] and the lowest half-maximal inhibitory concentration [IC 50 ; 1.17 mg/ml in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2.58 mg/ml in 2,2-azino-bis (3-ethylbenzothiazo-line-6-sulfonic acid) diammonium salt (ABTS)].Conclusion Our study showed that T. triandra could inhibit the infectivity of PRRSV, whereas C. sappan Linn was the most effective for inhibiting PRRSV replication in MARC-145 cells. This study elucidates the antiviral activities of Thai medicinal plant extracts in vivo . The result promising that the extracts of Thai medicinal plants, especially that of T. triandra and C. sappan Linn, can be developed into pharmaceutical drugs for the prevention of PRRS in pigs.

scholarly journals Modulation of Streptomycin Killing Rate against Mature Escherichia Coli Biofilms in the Presence of Medicinal Plant Extracts

2019 ◽  
Vol 4 (5) ◽  
pp. 50-54
Author(s):  
Z. Y. Samoilova ◽  
G. V. Smirnova ◽  
O. N. Oktyabrsky
Keyword(s):  
Escherichia Coli ◽  
Medicinal Plant ◽  
Plant Extracts ◽  
Biofilm Formation ◽  
Antioxidant Properties ◽  
Laminaria Japonica ◽  
Black Tea ◽  
Killing Rate ◽  
Medicinal Plant Extracts ◽  
High Doses

Background. Medicinal plant extracts exhibiting pro- and antioxidant properties may affect antibiotic-induced killing of biofilm-producing bacteria in both synergistic and antagonistic modes. Better understanding of these alternations is required to adjust antibiotic therapy and herbal medicine in order to exclude unwanted losses of antibiotic efficiency.Aim: to study modulation modes of streptomycin killing rate against mature biofilms of Escherichia coli in the presence of different doses of commonly used medicinal plant extracts.Materials and methods. Pharmacodynamic parameter killing rate and mass biofilm formation were determined in the presence of streptomycin and medicinal plant extracts.Results. Synergism was found between 100 mg/ml streptomycin and low doses (0.83 mg of dry herb/ml) of green, black tea, Arctostaphylos uva-ursi, Betula pendula and Laminaria japonica against killing mature biofilms. Alternatively, high doses (6.64 mg of dry herb/ml) of green, black tea and Vaccinium vitis-ideae demonstrated antagonism, decreasing killing rate and enhancing biofilm formation. Presumably, high doses of the extracts were sufficient to enhance biofilm formation blocking penetration of streptomycin through enlarged biofilm matrix and diminishing the killing rate.Conclusions. Widely consumed as soft beverages or for prophylactic purposes green, black tea and V. vitis-ideae could promote strong antagonistic effects with streptomycin. These extracts can stimulate biofilm production, making benefit for commensal microbiota, but have clinical relevance due to a significant reduction in the lethal efficiency of streptomycin in biofilms of pathogenic strains. This highlights the need of careful antibiotic prescription scheme adjustment when choosing appropriate combinations of plant extracts and antibiotics to achieve a synergistic effect.

Inhibition of Advanced Glycation End Product Formation by Medicinal Plant Extracts Correlates with Phenolic Metabolites and Antioxidant Activity

Planta Medica ◽  
2010 ◽  
Vol 77 (02) ◽  
pp. 196-204 ◽  
Author(s):  
Cory Harris ◽  
Louis-Philippe Beaulieu ◽  
Marie-Hélène Fraser ◽  
Kristina McIntyre ◽  
Patrick Owen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Plant Extracts ◽  
Product Formation ◽  
Advanced Glycation ◽  
Phenolic Metabolites ◽  
Advanced Glycation End Product ◽  
Medicinal Plant Extracts
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