scholarly journals In vitro Screening Antiviral Activity of Thai Medicinal Plants Against Porcine Reproductive and Respiratory Syndrome Virus

2020 ◽  
Author(s):  
Chaiwat Arjin ◽  
Kidsadagon Pringproa ◽  
Surat Hongsibsong ◽  
Warintorn Ruksiriwanich ◽  
Mintra Seel-audom ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Plant Extracts ◽  
Antioxidant Properties ◽  
Virus Titer ◽  
Economic Losses ◽  
Respiratory Syndrome Virus ◽  
Total Phenol Content ◽  
Medicinal Plant Extracts ◽  
Thai Medicinal Plants

Abstract Background Porcine reproductive and respiratory syndrome (PRRS) caused by the PRRS virus (PRRSV) results in economic losses in the swine industry globally. Several studies have investigated the use of plant extracts for the prevention and control of PRRS outbreaks. Thai medicinal plants may be useful for treating PRRSV infection in pigs. Therefore, we investigated the in vitro anti-PRRSV and antioxidant properties of seven Thai medicinal plants: Caesalpinia sappan Linn., Garcinia mangostana Linn., Houttuynia cordata , Perilla frutescens , Clinacanthus nutans , Phyllanthus emblica , and Tiliacora triandra .Results In the antiviral screening test, we observed that the T. triandra extract strongly inhibited the infectivity of PRRSV into MARC-145 cells [virus titer 3.5 median tissue culture infectious doses (TCID 50 )/ml (log 10 )] at 24 h post-infection, whereas the C. sappan Linn. extract strongly inhibited PRRSV replication [virus titer 2.5 TCID 50 /ml (log 10 )] at 72 h postinfection. C. sappan Linn. extract had the highest total phenol content [220.52 mM gallic acid equivalents (GAE)/g] and the lowest half-maximal inhibitory concentration [IC 50 ; 1.17 mg/ml in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2.58 mg/ml in 2,2-azino-bis (3-ethylbenzothiazo-line-6-sulfonic acid) diammonium salt (ABTS)].Conclusion Our study showed that T. triandra could inhibit the infectivity of PRRSV, whereas C. sappan Linn was the most effective for inhibiting PRRSV replication in MARC-145 cells. This study elucidates the antiviral activities of Thai medicinal plant extracts in vivo . The result promising that the extracts of Thai medicinal plants, especially that of T. triandra and C. sappan Linn, can be developed into pharmaceutical drugs for the prevention of PRRS in pigs.

scholarly journals Determination of Sun Protective Factor of Selected Medicinal Plants from Western Nepal

2020 ◽  
Vol 41 (1) ◽  
pp. 51-55
Author(s):  
Suman Prakash Pradhan ◽  
Kapil Adhikari ◽  
Saroj Nepal ◽  
Bishnu Prasad Pandey
Keyword(s):  
Medicinal Plants ◽  
Plant Extracts ◽  
Protective Factor ◽  
Absolute Methanol ◽  
Melia Azedarach ◽  
Asparagus Racemosus ◽  
Protective Agent ◽  
Protection Factor ◽  
Medicinal Plant Extracts

Plant extracts contain active ingredients that can absorb, reflect or scatter the sunlight, depending on their nature. The effectiveness of plant extracts as a skin protective agent can be determined by measuring the sun protection factor (SPF). The main aim of this study was to investigate the potential of selected medicinal plant extracts as a component in sunscreen production in modern cosmetics. The absolute methanol extract of six medicinal plants namely; Asparagus racemosus, Bergenia pacumbis, Melia azedarach, Murraya koenigii, Pleurospermum benthamii, and Thymus linearis were examined in-vitro for their sun protective ability by ultraviolet-visible spectrophotometry method with the application of Mansur equation. Our results revealed that most of the plant extracts possess prodigious SPF values as compared with commercial sunscreen. The greatest SPF value was found in P. benthamii (34.97±0.25), T. linearis (24.98±0.60), and B. pacumbis (24.02±0.15). These results show that these plant extracts can act as a very good antisolar agent.

scholarly journals In vitro screening antiviral activity of Thai medicinal plants against porcine reproductive and respiratory syndrome virus

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Chaiwat Arjin ◽  
Kidsadagon Pringproa ◽  
Surat Hongsibsong ◽  
Warintorn Ruksiriwanich ◽  
Mintra Seel-audom ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Antiviral Activity ◽  
Respiratory Syndrome Virus ◽  
In Vitro Screening ◽  
Thai Medicinal Plants

scholarly journals Evaluating the Antifungal Activity of Some Traditional Medicinal Plant Extracts against Alternaria solani (Tomato Early Blight Pathogen) in Ethiopia

2022 ◽  
Author(s):  
Meseret Tadelo ◽  
Tamirat Wato ◽  
Tilahun Negash
Keyword(s):  
Medicinal Plants ◽  
Plant Extracts ◽  
Early Blight ◽  
Allium Sativum ◽  
Alternaria Solani ◽  
Significant Inhibition ◽  
Crude Extracts ◽  
Medicinal Plant Extracts

Background: Tomato (Lycopersicon esculentum Mill.) belongs to the family Solanaceae. In Ethiopia, control of early blight is largely dependent on fungicidal application. There is a research need to identify effective botanical extracts to control Alternaria solani that cause early blight of tomato and for evaluation of plant extracts through different solvents on the target pathogen. Methods: In vitro experiment was conducted to evaluate the effectiveness of crude extracts of 16 selected medicinal plants against Alternaria solani. Thus, crude extracts were extracted from medicinal plants with different solvents (methanol, ethanol and petroleum at (25%, 50% and 100%) concentrations. The Alternaria solani was isolated from infected tomato leaves showing early blight symptoms. Evaluation of plant extracts was carried out against Alternaria solani using food poisoned technique on PDA. Result: Results showed that most of the methanolic extract plants were showed significant inhibition of the mycelial growth as compared to ethanolic and petroleum ether extracts. A higher rate of mycelial reduction was recorded by ethanol extracts of Allium sativum at all concentrations (100%) followed by methanol extracts of Allium sativum at 25%, 50%, 100% concentration (90.02%, 97.01%, 100% respectively). The effectiveness of extracts against Alternaria solani depends on use at the higher concentrations and various solvents. For crude extracts that have shown higher inhibitory effects against Alternaria solani in vitro conditions, actual chemical compounds should be identified. Furthermore, it is also important to evaluate these plants on other microbes, study to test in vivo and to assess their real potential field condition wherever early blight is an important disease of tomato.

scholarly journals Inhibition of Key Citrus Postharvest Fungal Strains by Plant Extracts In Vitro and In Vivo: A Review

Plants ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 26 ◽  
Author(s):  
Jinyin Chen ◽  
Yuting Shen ◽  
Chuying Chen ◽  
Chunpeng Wan
Keyword(s):  
Plant Extracts ◽  
Chemical Constituents ◽  
Alternative Methods ◽  
Economic Losses ◽  
Citrus Fruits ◽  
Edible Plant ◽  
Citrus Diseases ◽  
Medicinal Plant Extracts

Citrus fruits are subjected to a diversity of postharvest diseases caused by various pathogens during picking, packing, storage and transportation. Green and blue molds, caused by Penicillium digitatum and Penicillium italicum, respectively, are two major postharvest citrus diseases and cause significant economic losses during the commercialization phase. Currently, the control of postharvest citrus diseases relies mainly on the use of synthetic fungicides, which usually result in the resistance against fungal attack, environment pollution and health hazards. In recent years, much attention has been given to the preservation of citrus by naturally isolated edible plant extracts, medicinal plant extracts, Citrus extracts and volatiles, et al. Scientists worldwide devote their time and energy to discover the high effect, low toxicity, safety and inexpensive plant-derived fungicides. The current review will highlight plant-derived fungicides and chemical constituents that aim to inhibit P. digitatum and P. italicum in vitro and in vivo. Coatings enriched with plant extracts could be good alternative methods for Citrus fruits preservation. Problems and prospects of the research and development of plant-derived natural fungicides will also be discussed in this article.

scholarly journals Effect of Ethanolic Caesalpinia sappan Fraction on In Vitro Antiviral Activity against Porcine Reproductive and Respiratory Syndrome Virus

2021 ◽  
Vol 8 (6) ◽  
pp. 106
Author(s):  
Chaiwat Arjin ◽  
Surat Hongsibsong ◽  
Kidsadagon Pringproa ◽  
Mintra Seel-audom ◽  
Warintorn Ruksiriwanich ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Antiviral Activity ◽  
Crude Extract ◽  
Biological Activities ◽  
Virus Titer ◽  
Preparative Chromatography ◽  
Economic Losses ◽  
Respiratory Syndrome Virus ◽  
Caesalpinia Sappan

Porcine reproductive and respiratory syndrome virus (PRRSV) is a major epidemic in pig production, leading to economic losses in the pig industry worldwide. The use of medicinal plants with antiviral properties might be useful help to prevent and control PRRSV outbreaks. Caesalpinia sappan (CS) heartwood is an important herbal ingredient used in Thai folk medicine, possessing various biological activities, including antiviral activity. The present study focuses on the in vitro antiviral activity against PRRSV of a semi-purified fraction of ethanolic CS crude extract using preparative high-performance liquid chromatography. Qualification of the fractions illustrating positive antiviral activity was carried out with liquid chromatography–quadrupole time-of-flight mass spectrometry. The preparative chromatography separated the crude extract into six consecutive fractions, among which the first fraction showed potential antiviral activity by inhibiting PRRSV replication in a MARC-145 monolayer (virus titer 2.75 median tissue culture infective dose (TCID50)/mL (log10) vs. 9.50 median log10 TCID50/mL of the control) at 72 h post-infection, and this fraction included byakangelicin, brazilin, naringenin, and brazilein. These results provide useful information for further study to effectively develop the CS bioactive antiviral compounds against PRRSV as a feed additive or veterinary drug in the pig industry.

Natural products from the traditional Moroccan pharmacopoeia: A potential lead for the prevention and treatment of COVID-19

2020 ◽  
Vol 11 (SPL1) ◽  
pp. 1278-1285
Author(s):  
Mohamed Yafout ◽  
Amine Ousaid ◽  
Ibrahim Sbai El Otmani ◽  
Youssef Khayati ◽  
Amal Ait Haj Said
Keyword(s):  
Medicinal Plants ◽  
Plant Extracts ◽  
Molecular Mechanisms ◽  
Scientific Literature ◽  
Treatment Protocol ◽  
World Health ◽  
The World ◽  
Health Organization ◽  
Promising Source

The new SARS-CoV-2 belonging to the coronaviruses family has caused a pandemic affecting millions of people around the world. This pandemic has been declared by the World Health Organization as an international public health emergency. Although several clinical trials involving a large number of drugs are currently underway, no treatment protocol for COVID-19 has been officially approved so far. Here we demonstrate through a search in the scientific literature that the traditional Moroccan pharmacopoeia, which includes more than 500 medicinal plants, is a fascinating and promising source for the research of natural molecules active against SARS-CoV-2. Multiple in-silico and in-vitro studies showed that some of the medicinal plants used by Moroccans for centuries possess inhibitory activity against SARS-CoV or SARS-CoV-2. These inhibitory activities are achieved through the different molecular mechanisms of virus penetration and replication, or indirectly through stimulation of immunity. Thus, the potential of plants, plant extracts and molecules derived from plants that are traditionally used in Morocco and have activity against SARS-CoV-2, could be explored in the search for a preventive or curative treatment against COVID-19. Furthermore, safe plants or plant extracts that are proven to stimulate immunity could be officially recommended by governments as nutritional supplements.

scholarly journals Medicinal Plant Extracts

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 838
Author(s):  
Laura Grațiela Vicaș ◽  
Mariana Eugenia Mureșan
Keyword(s):  
Medicinal Plants ◽  
Medicinal Plant ◽  
Plant Extracts ◽  
Important Data ◽  
Therapeutic Benefits ◽  
Medicinal Plant Extracts

The therapeutic benefits of medicinal plants are well known and have been collected as important data on ethnomedicine [...]

scholarly journals Antibiotic sensitivity and in vitro antimicrobial activity of plant extracts to pseudomonas fluorescens isolates collected from diseased fish

1970 ◽  
Vol 1 (4) ◽  
pp. 82-88 ◽  
Author(s):  
MJ Foysal ◽  
MM Rahman ◽  
M Alam
Keyword(s):  
Antibacterial Activity ◽  
Pseudomonas Fluorescens ◽  
Plant Extracts ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Leaf Extracts ◽  
Sensitivity Pattern ◽  
Medicinal Plant Extracts

Studies were conducted to identify Pseudomonas fluorescens isolates from a collection of bacteria isolated from bacterial haemorrhagic septicaemia infected carp and catfish, evaluate their antibiotic sensitivity pattern and screen the antibacterial activity of some medicinal plant extracts against the isolates.. A total of 10 isolates were identified as P. fluorescens by morphological, physiological and biochemical tests. In vitro antibiotic sensitivity test of the P. fluorescens isolates were conducted by disc diffusion method for seven antibiotics where, all of the isolates were found to be sensitive only against streptomycin and gentamycin but, most of the isolates (80%) were found resistant to chloramphenicol (C). Moreover, eighty percent of the isolates showed resistance to multiple antibiotics. A total of 118 plant extracts were screened for their antibacterial activity against the P. fluorescens isolates where the isolates exhibited sensitivity to 30 samples. Leaf extracts of Tamarindus indicus, Terminalia chebula, Citrus aurantifolia, Eugenia caryophyllata and Spondias pinnata were found to inhibit the growth of all of the P. fluorescens isolates. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9733 IJNS 2011 1(4): 82-88

scholarly journals Biological activity from indigenous medicinal plants of Mauritius

2005 ◽  
Vol 77 (1) ◽  
pp. 41-51 ◽  
Author(s):  
A. Gurib-Fakim ◽  
H. Subratty ◽  
F. Narod ◽  
J. Govinden-Soulange ◽  
F. Mahomoodally
Keyword(s):  
Medicinal Plant ◽  
Plant Extracts ◽  
Biological Properties ◽  
Pharmacological Properties ◽  
Plant Materials ◽  
In Vivo Screening ◽  
Medicinal Plant Extracts ◽  
Minor Ailments

The Mauritian population has a long tradition in the use of ethno-medicine, and the practice is still strong, especially in the treatment of minor ailments. Such interest stems from an existing culture, and many “tisanes” are still prepared from plant materials and sold in several markets around the island.This paper will focus on the various chemical/biological screening techniques currently being used to evaluate the biological properties of medicinal plant extracts. Particular emphasis will be put on extraction and various screening for biological/pharmacological properties. Due consideration will be given to the pharmacological approaches that utilize different animal models for the in vitro and in vivo screening of medicinal plant extracts.

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