scholarly journals Characterization of novel pollen-expressed transcripts reveals their potential roles in pollen heat stress response in Arabidopsis thaliana

2020 ◽  
Author(s):  
Nicholas Rutley ◽  
Laetitia Poidevin ◽  
Tirza Doniger ◽  
Richard Tillet ◽  
Abhishek Rath ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Heat Stress ◽  
Regulatory Networks ◽  
Male Gametophyte ◽  
Open Reading Frames ◽  
Heat Stress Response ◽  
Rna Fragments ◽  
Response To Stress ◽  
Small Open Reading Frames

ABSTRACTThe male gametophyte is the most heat-sensitive of all plant tissues. In recent years, long noncoding RNAs (lncRNAs) have emerged as important components of cellular regulatory networks involved in most biological processes, including response to stress. While examining RNAseq datasets of developing and germinating Arabidopsis thaliana pollen exposed to heat stress (HS), we identified 66 novel and 246 recently-annotated intergenic expressed loci (XLOCs) of unknown function, with the majority encoding lncRNAs. Comparison to HS in cauline leaves and other RNAseq experiments, indicated 74% of the 312 XLOCs are pollen-specific, and at least 42% are HS-responsive. Phylogenetic analysis revealed 96% of the genes evolved recently in Brassicaceae. We found that 50 genes are putative targets of microRNAs, and that 30% of the XLOCs contain small open reading frames (ORFs) with homology to protein sequences. Finally, RNAseq of ribosome-protected RNA fragments together with predictions of periodic footprint of the ribosome P-sites indicated that 23 of these ORFs are likely to be translated. Our findings indicate that many of the 312 unknown genes might be functional, and play significant role in pollen biology, including the HS response.

scholarly journals Characterization of novel pollen-expressed transcripts reveals their potential roles in pollen heat stress response in Arabidopsis thaliana

2021 ◽  
Vol 34 (1) ◽  
pp. 61-78
Author(s):  
Nicholas Rutley ◽  
Laetitia Poidevin ◽  
Tirza Doniger ◽  
Richard L. Tillett ◽  
Abhishek Rath ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Heat Stress ◽  
Stress Response ◽  
Unknown Function ◽  
Regulatory Networks ◽  
Male Gametophyte ◽  
Open Reading Frames ◽  
Heat Stress Response ◽  
Rna Fragments ◽  
Response To Stress

Abstract Key message Arabidopsis pollen transcriptome analysis revealed new intergenic transcripts of unknown function, many of which are long non-coding RNAs, that may function in pollen-specific processes, including the heat stress response. Abstract The male gametophyte is the most heat sensitive of all plant tissues. In recent years, long noncoding RNAs (lncRNAs) have emerged as important components of cellular regulatory networks involved in most biological processes, including response to stress. While examining RNAseq datasets of developing and germinating Arabidopsis thaliana pollen exposed to heat stress (HS), we identified 66 novel and 246 recently annotated intergenic expressed loci (XLOCs) of unknown function, with the majority encoding lncRNAs. Comparison with HS in cauline leaves and other RNAseq experiments indicated that 74% of the 312 XLOCs are pollen-specific, and at least 42% are HS-responsive. Phylogenetic analysis revealed that 96% of the genes evolved recently in Brassicaceae. We found that 50 genes are putative targets of microRNAs and that 30% of the XLOCs contain small open reading frames (ORFs) with homology to protein sequences. Finally, RNAseq of ribosome-protected RNA fragments together with predictions of periodic footprint of the ribosome P-sites indicated that 23 of these ORFs are likely to be translated. Our findings indicate that many of the 312 unknown genes might be functional and play a significant role in pollen biology, including the HS response.

scholarly journals Correction to: Characterization of novel pollen-expressed transcripts reveals their potential roles in pollen heat stress response in Arabidopsis thaliana

2021 ◽  
Vol 34 (1) ◽  
pp. 79-79
Author(s):  
Nicholas Rutley ◽  
Laetitia Poidevin ◽  
Tirza Doniger ◽  
Richard L. Tillett ◽  
Abhishek Rath ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Heat Stress ◽  
Stress Response ◽  
Heat Stress Response

scholarly journals Grapevine Responses to Heat Stress and Global Warming

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1754
Author(s):  
Xenophon Venios ◽  
Elias Korkas ◽  
Aspasia Nisiotou ◽  
Georgios Banilas
Keyword(s):  
Global Warming ◽  
Heat Stress ◽  
Regulatory Networks ◽  
High Temperature Stress ◽  
Extreme Weather Events ◽  
Wine Quality ◽  
Heat Stress Response ◽  
Average Annual Temperature ◽  
Current Century ◽  
Almost All

The potential effects of the forthcoming climate change include the rising of the average annual temperature and the accumulation of extreme weather events, like frequent and severe heatwaves, a phenomenon known as global warming. Temperature is an important environmental factor affecting almost all aspects of growth and development in plants. The grapevine (Vitis spp.) is quite sensitive to extreme temperatures. Over the current century, temperatures are projected to continue rising with negative impacts on viticulture. These consequences range from short-term effects on wine quality to long-term issues such as the suitability of certain varieties and the sustainability of viticulture in traditional wine regions. Many viticultural zones, particularly in Mediterranean climate regions, may not be suitable for growing winegrapes in the near future unless we develop heat-stress-adapted genotypes or identify and exploit stress-tolerant germplasm. Grapevines, like other plants, have developed strategies to maintain homeostasis and cope with high-temperature stress. These mechanisms include physiological adaptations and activation of signaling pathways and gene regulatory networks governing heat stress response and acquisition of thermotolerance. Here, we review the major impacts of global warming on grape phenology and viticulture and focus on the physiological and molecular responses of the grapevine to heat stress.

scholarly journals Functional characterization of Arabidopsis thaliana WRKY39 in heat stress

2010 ◽  
Vol 29 (5) ◽  
pp. 475-483 ◽  
Author(s):  
Shujia Li ◽  
Xiang Zhou ◽  
Ligang Chen ◽  
Weidong Huang ◽  
Diqiu Yu
Keyword(s):  
Arabidopsis Thaliana ◽  
Heat Stress ◽  
Functional Characterization

scholarly journals Identification of small open reading frames (sORFs) associated with heat tolerance in nitrogen-fixing root nodules of Phaseolus vulgaris wild-type and cv BAT93

2021 ◽  
pp. 28-37
Author(s):  
Alejandra Zayas-del Moral ◽  
Damián Martínez- Reyes ◽  
Carmen Quinto ◽  
Federico Sanchez ◽  
Claudia Díaz- Camino
Keyword(s):  
Heat Stress ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Low Income ◽  
Expression Profiles ◽  
Open Reading Frames ◽  
Nitrogen Fixing ◽  
Next Generation Sequencing Technology ◽  
Reading Frames ◽  
Small Open Reading Frames

Common bean is an important legume crop and a major source of protein for low-income groups around the world. Legumes have the ability to engage symbiotic interactions with nitrogen-fixing soil bacteria. In this study, next-generation sequencing technology was used to perform transcriptome analyses of a yet unexplored group of peptides encoded by small open reading frames (sORFs; < 150 codons) in nitrogen-fixing symbiotic nodules of two heat-tolerant genotypes of common bean (Phaseolus vulgaris L): the cultivar BAT93 and a wild genotype (named P. vulgaris 7) from the south of Mexico. After heat stress, total RNA was isolated and used for transcriptome analysis. Sixty differentially expressed sORFs were identified between control and heat stress treatments. The expression profiles of these sORFs suggest that, regardless the evolutionary closeness between P. vulgaris BAT93 and P. vulgaris 7, each genotype has independently adapted their molecular signaling pathways to survive heat stress. The dataset developed may provide a useful resource for future genetic and genomic studies in these species

Sign in / Sign up

Export Citation Format

Share Document