scholarly journals Hydrogel-coating improves the in-vivo stability of electrochemical aptamer-based biosensors

2020 ◽  
Author(s):  
Shaoguang Li ◽  
Jun Dai ◽  
Man Zhu ◽  
Netzahualcóyotl Arroyo-Currás ◽  
Hongxing Li ◽  
...  
Keyword(s):  
Real Time ◽  
Response Times ◽  
Protective Layer ◽  
Drift Correction ◽  
Living Body ◽  
Agarose Hydrogel ◽  
Improved Stability ◽  
Varying Environments

AbstractThe ability to track the levels of specific molecules, such as drugs, metabolites, and biomarkers, in the living body, in real time and for long durations would improve our understanding of health and our ability to diagnose, treat and monitor disease. To this end, we are developing electrochemical aptamer-based (E-AB) biosensors, a general platform supporting high-frequency, real-time molecular measurements in the living body. Here we report that the addition of an agarose hydrogel protective layer to E-AB sensors significantly improves their baseline stability when deployed in the complex, highly time-varying environments found in vivo. The improved stability is sufficient that these hydrogel-protected sensors achieved good baseline stability when deployed in situ in the veins, muscles, bladder, or tumors of living rats without the use of the drift correction approaches traditionally required in such placements. Finally, this improved stability is achieved without any significant, associated “costs” in terms of detection limits, response times, or biocompatibility.

Hybrid MPI-MRI System for Dual-Modal In Situ Cardiovascular Assessments of Real-Time 3D Blood Flow Quantification—A Pre-Clinical In Vivo Feasibility Investigation

2020 ◽  
Vol 39 (12) ◽  
pp. 4335-4345
Author(s):  
Jochen Franke ◽  
Nicoleta Baxan ◽  
Heinrich Lehr ◽  
Ulrich Heinen ◽  
Sebastian Reinartz ◽  
...  
Keyword(s):  
Blood Flow ◽  
Real Time ◽  
Flow Quantification ◽  
Blood Flow Quantification

scholarly journals Seconds-resolved pharmacokinetic measurements of the chemotherapeutic irinotecan in situ in the living body

2019 ◽  
Vol 10 (35) ◽  
pp. 8164-8170 ◽  
Author(s):  
Andrea Idili ◽  
Netzahualcóyotl Arroyo-Currás ◽  
Kyle L. Ploense ◽  
Andrew T. Csordas ◽  
Masayasu Kuwahara ◽  
...  
Keyword(s):  
Real Time ◽  
The Body ◽  
Living Body

The ability to measure drugs in the body rapidly and in real time would advance both our understanding of pharmacokinetics and our ability to optimally dose and deliver pharmacological therapies.

In vivo target bio-imaging of cerebral ischemic stroke by real-time labeling of zinc

RSC Advances ◽  
2016 ◽  
Vol 6 (112) ◽  
pp. 110525-110534 ◽  
Author(s):  
Chunqiu Zhao ◽  
Lanmei Lai ◽  
Fawad Ur Rehman ◽  
Cheng Qian ◽  
Gaojun Teng ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Real Time ◽  
Intravenous Injection ◽  
Brain Regions ◽  
Zinc Gluconate ◽  
Bio Imaging ◽  
Cerebral Ischemic Stroke ◽  
Cerebral Ischemic

Through intravenous injection of zinc gluconate, we could readily realize in vivo fluorescence imaging by real-time labeling the relevant brain regions of CIS model mice based on the in situ biosynthesis of fluorescence zinc nanoclusters in target diseased sites.

scholarly journals Novel Instrumentation for Real-Time Monitoring Using Miniaturized Flow Systems with Integrated Biosensors

1997 ◽  
Vol 34 (3) ◽  
pp. 291-302 ◽  
Author(s):  
R Freaney ◽  
A McShane ◽  
T V Keaveny ◽  
M McKenna ◽  
K Rabenstein ◽  
...  
Keyword(s):  
Real Time ◽  
Response Times ◽  
Computer Software ◽  
Dead Volume ◽  
System Response ◽  
Small Scale ◽  
Biosensor Array ◽  
In Vivo Testing ◽  
On Line

A prototype miniaturized Total Chemical Analysis System (μTAS) has been developed and applied to on-line monitoring of glucose and lactate in the core blood of anaesthetized dogs. The system consists of a highly efficient microdialysis sampling interface sited in a small-scale extracorporeal shunt circuit (‘MiniShunt’), a silicon machined microflow manifold and integrated biosensor array for glucose and lactate detection with associated computer software for analytical process control. During in-vivo testing the device allowed real-time on-screen monitoring of glucose and lactate with system response times of less than 5 min, made possible by the small dead volume of the microflow system. On-line glucose and lactate measurements were made in the basal state as well as during intravenous infusion of glucose or lactate. The prototype μTAS is currently suitable for trend monitoring but refinements are necessary before application of the system for determination of individual lactate values.

Real-time measurement of non-lethal platelet thromboembolic responses in the anaesthetized mouse

2008 ◽  
Vol 99 (02) ◽  
pp. 435-440 ◽  
Author(s):  
Charalambos Tymvios ◽  
Sarah Jones ◽  
Christopher Moore ◽  
Simon C. Pitchford ◽  
Clive P. Page ◽  
...  
Keyword(s):  
Real Time ◽  
Platelet Function ◽  
Thrombus Formation ◽  
Inhibitory Effect ◽  
Pulmonary Vasculature ◽  
Real Time Measurement ◽  
Platelet Aggregates ◽  
Dose Responses

SummaryIdentifying and evaluating new therapeutic targets in platelets requires advanced animal models in which platelet responses can be measured directly and in situ.This is important because platelet function is strongly influenced by external factors such as those originating from the vascular endothelium. Our objectives were to record graded, non-lethal thromboembolic platelet responses to platelet agonists in situ in the mouse and to demonstrate an inhibitory effect of aspirin in our model. Radiolabelled platelets were infused into anaesthetized mice and responses to ADP, collagen and thrombin measured as changes in platelet associated counts in miniaturized detection probes placed over the thoracic region. All agonists induced dose-dependent changes in platelet counts due to accumulation of thrombi in the pulmonary vasculature. We confirmed a specific platelet effect by comparing platelet and erythrocyte responses and showing platelet aggregates in the lung vasculature histologically. Simultaneous injection of collagen and adrenaline induced increased and protracted synergistic platelet responses compared with individual injection of these agents and aspirin inhibited collagen-induced responses. We confirmed the clinical relevance of our model by showing that platelet thromboembolism in the mouse, like pulmonary embolism in humans, impaired cardiovascular performance. We present a refined method for measuring platelet agonist dose-responses and thromboembolism in real-time without inducing mortality in the mouse. Our technique will be useful in investigating the molecular determinants of physiological and pathophysiological platelet function in an in-vivo context and will enable investigations of both platelet and non-platelets mediators of thrombus formation.

scholarly journals A Nano-Platform for in situ Investigations of Ovarian Follicles

2020 ◽  
Author(s):  
Jean M. Feugang ◽  
Ghassan Ishak ◽  
Matthew Eggert ◽  
Robert Arnold ◽  
Scott Willard ◽  
...  
Keyword(s):  
Real Time ◽  
Transvaginal Ultrasound ◽  
Follicle Cell ◽  
Ovarian Follicles ◽  
Follicle Cells ◽  
Reproductive Techniques ◽  
Cell Layers ◽  
Doxorubicin Delivery

Abstract Background: Despite the growing array of assisted reproductive techniques, there is still a lack of rapid, non-cytotoxic, and minimally invasive in situ approaches for further enhancements through cell targeting. Here we synthesized clinically relevant liposome nanoparticles for real-time cellular targeting and drug (doxorubicin) delivery, using pigs and mares as animal models. In Experiment 1, fluorescently labeled and doxorubicin-loaded (without fluorescent probe) liposomes were injected in cultured pig ovarian follicles to assess plasma membrane binding and intracellular doxorubicin delivery. In Experiment 2, fluorescent liposomes were in vivo injected into small and large ovarian follicles of living mares to assess their binding capability to follicular cells. Twenty-four hours post-injection, all cultured pig follicles were collected while mare samples (i.e., follicle wall fragments, granulosa cells and follicular fluids) were harvested through follicle wall biopsy (FWB), and follicle aspiration and flushing techniques using transvaginal ultrasound-guided approach.Results: All injected follicles were healthy and samples were subjected to fluorescence imaging before and after fixation. Findings revealed successful intrafollicular migration and binding of liposomes to all follicle cell layers (granulosa, theca interna, and theca externa) regardless of the follicle size. The intracellular delivery of doxorubicin was confirmed with the staining of nuclei of follicle cells. Conclusions: This study demonstrates the promising combination of the FWB technique and nanotechnology tools for real-time monitoring of intrafollicular treatment, follicular health and oocyte development, which in turn has the potential to help understand the mechanisms of ovulatory dysfunction and to select high-quatity oocytes for assisted reproduction techniques.

Near-infrared ratiometric self-assembled theranostic nanoprobe: imaging and tracking cancer chemotherapy

2020 ◽  
Vol 56 (25) ◽  
pp. 3629-3632 ◽  
Author(s):  
Xinwei Tian ◽  
Zhao Li ◽  
Ning Ding ◽  
Jiahang Zhang
Keyword(s):  
Cancer Therapy ◽  
Real Time ◽  
Cancer Chemotherapy ◽  
Near Infrared ◽  
Self Assembled

A novel near-infrared ratiometric fluorescent theranostic nanoprobe is applied for real-time fluorescence tracking and imaging cancer therapy in vivo and in situ.

In vivo and in situ real-time fluorescence imaging of peripheral nerves in the NIR-II window

Nano Research ◽  
2019 ◽  
Vol 12 (12) ◽  
pp. 3059-3068 ◽  
Author(s):  
Zhujun Feng ◽  
Yimeng Yang ◽  
Jian Zhang ◽  
Kan Wang ◽  
Yunxia Li ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescence Imaging ◽  
Peripheral Nerves

scholarly journals Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jooran Lee ◽  
Byungyeon Kim ◽  
Byungjun Park ◽  
Youngjae Won ◽  
Sang-Yeob Kim ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescence Lifetime ◽  
Normal Tissues ◽  
Diagnosis Of Cancer ◽  
Novel Method ◽  
Hematoxylin And Eosin ◽  
Cancer Tissues ◽  
The Relationship

AbstractA biopsy is often performed for the diagnosis of cancer during a surgical operation. In addition, pathological biopsy is required to discriminate the margin between cancer tissues and normal tissues in surgical specimens. In this study, we presented a novel method for discriminating between tumor and normal tissues using fluorescence lifetime endoscopy (FLE). We demonstrated the relationship between the fluorescence lifetime and pH in fluorescein using the proposed fluorescence lifetime measurement system. We also showed that cancer could be diagnosed based on this relationship by assessing differences in pH based fluorescence lifetime between cancer and normal tissues using two different types of tumor such as breast tumors (MDA-MB-361) and skin tumors (A375), where cancer tissues have ranged in pH from 4.5 to 7.0 and normal tissues have ranged in pH from 7.0 to 7.4. To support this approach, we performed hematoxylin and eosin (H&E) staining test of normal and cancer tissues within a certain area. From these results, we showed the ability to diagnose a cancer using FLE technique, which were consistent with the diagnosis of a cancer with H&E staining test. In summary, the proposed pH-based FLE technique could provide a real time, in vivo, and in-situ clinical diagnostic method for the cancer surgical and could be presented as an alternative to biopsy procedures.

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