pH fluctuations drive waves of stereotypical cellular reorganizations during entry into quiescence

AbstractThe life cycle of microorganisms is associated with dynamic metabolic transitions and complex cellular responses. In yeast, how metabolic signals control the progressive establishment of structural reorganizations observed in quiescent cells remains unclear. To address this question, we have developed a method that combines nutrient-limited proliferation assays at the population level with single-cell tracking to unravel the coordination between metabolic and structural transitions in cells during an unperturbed lifecycle. We show that non-monotonous internal pH fluctuations are in sync with successive waves of protein super-assemblies formation and ultimately lead to a cytosolic glass transition. Our results, therefore, suggest a simple model explaining how the complex developmental changes during the yeast life cycle are orchestrated by the sequence of metabolic transitions.

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Monitoring single-cell dynamics of entry into quiescence during an unperturbed lifecycle

eLife ◽

10.7554/elife.73186 ◽

2021 ◽

Vol 10 ◽

Author(s):

Basile Jacquel ◽

Théo Aspert ◽

Damien Laporte ◽

Isabelle Sagot ◽

Gilles Charvin

Keyword(s):

Life Cycle ◽

Single Cell ◽

Cell Fate ◽

Cell Tracking ◽

Cellular Responses ◽

Quiescent State ◽

Cell Dynamics ◽

Quiescent Cells ◽

Internal Ph ◽

Bona Fide

The life cycle of microorganisms is associated with dynamic metabolic transitions and complex cellular responses. In yeast, how metabolic signals control the progressive choreography of structural reorganizations observed in quiescent cells during a natural life cycle remains unclear. We have developed an integrated microfluidic device to address this question, enabling continuous single-cell tracking in a batch culture experiencing unperturbed nutrient exhaustion to unravel the coordination between metabolic and structural transitions within cells. Our technique reveals an abrupt fate divergence in the population, whereby a fraction of cells is unable to transition to respiratory metabolism and undergoes a reversible entry into a quiescence-like state leading to premature cell death. Further observations reveal that non-monotonous internal pH fluctuations in respiration-competent cells orchestrate the successive waves of protein super-assemblies formation that accompany the entry into a bona fide quiescent state. This ultimately leads to an abrupt cytosolic glass transition that occurs stochastically long after proliferation cessation. This new experimental framework provides a unique way to track single-cell fate dynamics over a long timescale in a population of cells that continuously modify their ecological niche.

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Upscaling site-scale ecohydraulic models to inform salmonid population-level life cycle modeling and restoration actions - Lessons from the Columbia River Basin

Earth Surface Processes and Landforms ◽

10.1002/esp.4137 ◽

2017 ◽

Vol 43 (1) ◽

pp. 21-44 ◽

Cited By ~ 14

Author(s):

Joseph M. Wheaton ◽

Nicolaas Bouwes ◽

Peter Mchugh ◽

Carl Saunders ◽

Sara Bangen ◽

...

Keyword(s):

Life Cycle ◽

River Basin ◽

Columbia River ◽

Population Level ◽

Columbia River Basin

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Probing the precipitation life cycle by iterative rain cell tracking

Journal of Geophysical Research Atmospheres ◽

10.1002/2013jd020868 ◽

2013 ◽

Vol 118 (24) ◽

pp. 13,361-13,370 ◽

Cited By ~ 25

Author(s):

Christopher Moseley ◽

Peter Berg ◽

Jan O. Haerter

Keyword(s):

Life Cycle ◽

Cell Tracking

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Phenology and seed production in Chenopodium rubrum, Rumex maritimus, and Rumex palustris as related to photoperiod in river forelands

Canadian Journal of Botany ◽

10.1139/b92-053 ◽

1992 ◽

Vol 70 (2) ◽

pp. 392-400 ◽

Cited By ~ 15

Author(s):

A. J. M. Van der Sman ◽

C. W. P. M. Blom ◽

H. M. Van de Steeg

Keyword(s):

Life Cycle ◽

Seed Production ◽

Population Level ◽

Flowering Phenology ◽

Chenopodium Rubrum ◽

Axillary Shoots ◽

Critical Photoperiod ◽

Number Of Leaves ◽

Rumex Maritimus ◽

Flooded Areas

Reproductive development in three species from irregularly flooded areas of river forelands was studied in relation to time of emergence. In Chenopodium rubrum, flowering was induced earlier in plants germinated in April–May than in later cohorts. However, the period of vegetative growth diminished and the life cycle was completed in a shorter time in later germinated plants. Seed number was reduced, but seed size as well as reproductive effort per plant increased in later cohorts. Plants of both Rumex species flowered after a certain number of leaves had developed and before a critical photoperiod had passed. In earlier cohorts, the main shoot and several axillary shoots elongated and flowered. Fewer axillary shoots flowered closer to the critical photoperiod, and this resulted in a reduced seed output in later cohorts. The critical photoperiod as well as the time needed for completion of the life cycle was longer in Rumex palustris than in Rumex maritimus. It is argued that in the riparian habitat, plants of both Rumex species are only occasionally able to complete their life cycle in one growing season. Survival of these species on the population level will rely more upon adaptations towards flooding during the established phase than is the case for C. rubrum. Key words: flowering phenology, photoperiod, seed production, Chenopodium rubrum, Rumex maritimus, Rumex palustris.

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Developmental changes in the molecular forms of acetylcholinesterase during the life cycle of the lamprey Petromyzon marinus

Comparative Biochemistry and Physiology Part B Comparative Biochemistry ◽

10.1016/0305-0491(93)90314-u ◽

1993 ◽

Vol 106 (2) ◽

pp. 369-372 ◽

Cited By ~ 1

Author(s):

Christopher Atkins ◽

Leo Pezzementi

Keyword(s):

Life Cycle ◽

Petromyzon Marinus ◽

Developmental Changes ◽

Molecular Forms

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Domestication reprogrammed the budding yeast life cycle

10.1101/2020.02.08.939314 ◽

2020 ◽

Cited By ~ 3

Author(s):

Matteo De Chiara ◽

Benjamin Barré ◽

Karl Persson ◽

Amadi Onyetuga Chioma ◽

Agurtzane Irizar ◽

...

Keyword(s):

Life Cycle ◽

Evolutionary History ◽

Budding Yeast ◽

World Population ◽

Model Species ◽

Quiescent Cells ◽

Gamete Formation ◽

The World ◽

Wild Yeasts ◽

Yeast Evolution

AbstractDomestication of plants and animals is the foundation for feeding the world population. We report that domestication of the model yeast S. cerevisiae reprogrammed its life cycle entirely. We tracked growth, gamete formation and cell survival across many environments for nearly 1000 genome sequenced isolates and found a remarkable dichotomy between domesticated and wild yeasts. Wild yeasts near uniformly trigger meiosis and sporulate when encountering nutrient depletions, whereas domestication relaxed selection on sexual reproduction and favoured survival as quiescent cells. Domestication also systematically enhanced fermentative over respiratory traits while decreasing stress tolerance. We show that this yeast domestication syndrome was driven by aneuploidies and gene function losses that emerged independently in multiple domesticated lineages during the specie’s recent evolutionary history. We found domestication to be the most dramatic event in budding yeast evolution, raising questions on how much domestication has distorted our understanding of this key model species.

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Using Life Cycle Models to Identify Monitoring Gaps for Central Valley Spring-Run Chinook Salmon

San Francisco Estuary and Watershed Science ◽

10.15447/sfews.2020v18iss4art3 ◽

2020 ◽

Vol 18 (4) ◽

Author(s):

Flora Cordoleani ◽

William Satterthwaite ◽

Miles Daniels ◽

Matthew Johnson

Keyword(s):

Life Cycle ◽

Oncorhynchus Tshawytscha ◽

Monitoring Program ◽

Central Valley ◽

Population Level ◽

Trapping Efficiency ◽

Genetic Identification ◽

Management Actions ◽

Data Gaps ◽

Life Cycle Models

Life-cycle models (LCMs) provide a quantitative framework that allows evaluation of how management actions targeting specific life stages can have population-level effects on a species. LCMs are particularly useful for species such as salmon that are highly migratory and use multiple aquatic ecosystems throughout their life. LCMs are lacking for threatened Central Valley spring-run Chinook (Oncorhynchus tshawytscha; CVSC), so the authors developed a CVSC LCM to describe the dynamics of Mill, Deer, and Butte creek CVSC populations. The authors propose a series of monitoring recommendations—such as the development of an enhanced juvenile-tracking monitoring program, and the implementation of juvenile-trapping efficiency methodology combined with genetic identification tools—to help fill highlighted data gaps.

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Whirling Disease: Reviews and Current Topics

Whirling Disease: Reviews and Current Topics ◽

10.47886/9781888569377.ch14 ◽

2002 ◽

Keyword(s):

Life Cycle ◽

Community Dynamics ◽

Population Level ◽

Control Measures ◽

Complex Life Cycle ◽

Future Research ◽

Whirling Disease ◽

Myxobolus Cerebralis ◽

Natural Systems ◽

And Function

ABSTRACT. The myxosporean parasite Myxobolus cerebralis is the causative agent of salmonid whirling disease. Containing its spread and limiting its effects in the Intermountain West will require judicious management programs, but such actions await a comprehensive understanding of the biology and ecology of this parasite and its hosts and how these elements interact; we do not yet know the weaknesses of this organism. To better guide efforts aimed at such an understanding, we assembled available information on the ecology of the parasite, organizing it into a conceptual model of its life cycle, to help foster understanding, focus future research, and lead eventually to a mathematical model for evaluating control measures. Myxobolus cerebralis has a complex life cycle with two obligate hosts, a salmonid fish and the oligochaete Tubifex tubifex, parasitized by the myxosporean and the actinosporean, respectively, and two infective “spore” stages, the myxospore and the triactinomyxon. This complexity is enhanced by the variable suitability of multiple salmonid species to serve as hosts, varying host suitability of genetic variants of T. tubifex, relatively recent introduction of M. cerebralis to North America, and unique traits of the parasite that preclude easy classification into conventional modeling categories. Much is known about the anatomy and function of myxospores and triactinomyxons from laboratory studies, but information on their distribution, abundance, and dispersal in natural systems is limited and based on indirect observations. Similarly, we understand development of the parasite within its hosts and resulting pathologies well but know little about host immune reactions and other mechanisms controlling proliferation within hosts or how environmental factors affect these defenses. Population-level effects on fish in natural systems have been quantified only rarely, where good prewhirling disease data exist, and effects on T. tubifex populations are unknown. Most rates and frequencies needed to infer relationships and model system dynamics have not been directly quantified in natural systems, but rapid progress is being made. Larger issues, including effects of M. cerebralis on community dynamics and ecosystem structure and function, have yet to be explored.

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