scholarly journals All-Flesh Tomato Regulated by Reduced Dosage of AFF Provides New Insights into Berry Fruit Evolution

2021 ◽  
Author(s):  
Lei Liu ◽  
Kang Zhang ◽  
JinRui Bai ◽  
Jinghua Lu ◽  
Xiaoxiao Lu ◽  
...  
Keyword(s):  
Developmental Process ◽  
Regulatory Region ◽  
Relative Activity ◽  
The Core ◽  
Core Node ◽  
Sequence Deletion ◽  
Solanaceae Species ◽  
Tomato Processing ◽  
High Level ◽  
Locule Tissue

ABSTRACTThe formation of locule gel is not only an important developmental process in tomato but also a typical characteristic of berry fruit. In this study, we collected a tomato material that produces all-flesh fruits (AFF), whose locule tissue remains in a solid state during fruit development. We built genetic populations to fine map the causal gene of AFF trait, investigate the function of AFF gene, and identified it as the causal locus conferring the locule gel formation. We determined the causal mutation as a 416-bp deletion that occurred in the promoter region of AFF, which reduces the expression dosage of AFF. The 416-bp deleted sequence has a high level of conservation among closely related Solanaceae species, as well as in the tomato population. The activity of the 416-bp deletion in down-regulating gene expression was further verified by the relative activity in a luciferase experiment. Furthermore, with the BC6 NIL materials, we reveal that the reduced expression dosage of AFF does not impact the normal development of seeds, while produces non-liquefied locule tissue, which is distinct from that of the normal tomatoes in terms of metabolic components. Based on these findings, we propose that the AFF gene is the core node in locule tissue liquefaction, whose function cannot be compensated by its paralogs TAG1, TAGL1, or TAGL11. Our findings provide clues to investigate fruit type differentiation among Solanaceae crops, and also contributes to the breeding application of all flesh fruit tomatoes for the tomato processing industry.One Sentence SummaryThe sequence deletion that occurred in the cis-regulatory region of AFF—the core node of locule tissue liquefaction determined here—reduced its expression dosage, and produced all flesh fruit tomato.

Consent

2018 ◽  
Author(s):  
Martin Krzywdzinski
Keyword(s):  
Production Systems ◽  
Employee Behavior ◽  
Labor Disputes ◽  
Performance Expectations ◽  
The Third ◽  
The Core ◽  
Core Requirements ◽  
High Level

This chapter deals with the dependent variable of the study: consent. It analyses workplace consent in Russia and China using three indicators that refer to the core requirements of the production systems in automotive companies regarding employee behavior: first, standardized work; and second, compliance with expectations in terms of flexibility, cooperation, and a commitment to improving processes. The third indicator of consent (or the lack of it) is the absence or presence of open criticism, resistance, and labor disputes. The chapter reveals significant and unexpected differences between the Chinese and Russian sites on all three indicators. While the Chinese factories exhibit (with some variance between the companies), a relatively high level of consent, the Russian plants have problems with standardized work, the acceptance of performance expectations, and to some extent with labor disputes.

Mutations Causing Constitutive Invertase Synthesis in Yeast: Genetic Interactions with snf Mutations

Genetics ◽  
1987 ◽  
Vol 115 (2) ◽  
pp. 247-253
Author(s):  
Lenore Neigeborn ◽  
Marian Carlson
Keyword(s):  
Regulatory Region ◽  
Constitutive Expression ◽  
Genetic Interactions ◽  
The Third ◽  
Recessive Mutations ◽  
Constitutive Mutation ◽  
Complementation Groups ◽  
High Level ◽  
Yeast Genetic ◽  
Very High

ABSTRACT We have selected 210 mutants able to grow on sucrose in the presence of 2-deoxyglucose. We identified recessive mutations in three major complementation groups that cause constitutive (glucose-insensitive) secreted invertase synthesis. Two groups comprise alleles of the previously identified HXK2 and REG1 genes, and the third group was designated cid1 (constitutive invertase derepression). The effect of cid1 on SUC2 expression is mediated by the SUC2 upstream regulatory region, as judged by the constitutive expression of a SUC2-LEU2-lacZ fusion in which the LEU2 promoter is under control of SUC2 upstream sequences. A cid1 mutation also causes glucose-insensitive expression of maltase. The previously isolated constitutive mutation ssn6 is epistatic to cid1, reg1 and hxk2 for very high level constitutive invertase expression. Mutations in SNF genes that prevent derepression of invertase are epistatic to cid1, reg1 and hxk2; we have previously shown that ssn6 has different epistasis relationships with snf mutations. The constitutive mutation tup1 was found to resemble ssn6 in its genetic interactions with snf mutations. These findings suggest that CID1, REG1 and HXK2 are functionally distinct from SSN6 and TUP1.

Short repeated elements in the upstream regulatory region of the SUC2 gene of Saccharomyces cerevisiae

1986 ◽  
Vol 6 (7) ◽  
pp. 2324-2333
Author(s):  
L Sarokin ◽  
M Carlson
Keyword(s):  
Carbon Catabolite Repression ◽  
Consensus Sequence ◽  
Regulatory Region ◽  
Lacz Fusion ◽  
Upstream Region ◽  
Upstream Regulatory Region ◽  
Heterologous Promoter ◽  
Suc2 Gene ◽  
High Level ◽  
Repeated Motif

Expression of secreted invertase from the SUC2 gene is regulated by carbon catabolite repression. Previously, an upstream regulatory region that is required for derepression of secreted invertase was identified and shown to confer glucose-repressible expression to the heterologous promoter of a LEU2-lacZ fusion. In this paper we show that tandem copies of a 32-base pair (bp) sequence from the upstream regulatory region activate expression of the same LEU2-lacZ fusion. The level of expression increased with the number of copies of the element, but was independent of their orientation; the expression from constructions containing four copies of the sequence was only twofold lower than that when the entire SUC2 upstream regulatory region was present. This activation was not significantly glucose repressible. The 32-bp sequence includes a 7-bp motif with the consensus sequence (A/C)(A/G)GAAAT that is repeated at five sites within the upstream regulatory region. Genetic evidence supporting the functional significance of this repeated motif was obtained by pseudoreversion of a SUC2 deletion mutant lacking part of the upstream region, including two copies of the 7-bp element. In three of five pseudorevertants, the mutations that restored high-level SUC2 expression altered one of the remaining copies of the 7-bp element.

TRU Recycling Options for Environmentally Friendly and Proliferation-Resistant Nuclear Fuel Cycle of FBR

2010 ◽  
Author(s):  
Sidik Permana ◽  
Mitsutoshi Suzuki
Keyword(s):  
High Performance ◽  
Fuel Cycle ◽  
Operation Time ◽  
Nuclear Nonproliferation ◽  
High Level Waste ◽  
Minor Actinide ◽  
Spent Fuel ◽  
Closed Fuel Cycle ◽  
The Core ◽  
High Level

The embodied challenges for introducing closed fuel cycle are utilizing advanced fuel reprocessing and fabrication facilities as well as nuclear nonproliferation aspect. Optimization target of advanced reactor design should be maintained properly to obtain high performance of safety, fuel breeding and reducing some long-lived and high level radioactivity of spent fuel by closed fuel cycle options. In this paper, the contribution of loading trans-uranium to the core performance, fuel production, and reduction of minor actinide in high level waste (HLW) have been investigated during reactor operation of large fast breeder reactor (FBR). Excess reactivity can be reduced by loading some minor actinide in the core which affect to the increase of fuel breeding capability, however, some small reduction values of breeding capability are obtained when minor actinides are loaded in the blanket regions. As a total composition, MA compositions are reduced by increasing operation time. Relatively smaller reduction value was obtained at end of operation by blanket regions (9%) than core regions (15%). In addition, adopting closed cycle of MA obtains better intrinsic aspect of nuclear nonproliferation based on the increase of even mass plutonium in the isotopic plutonium composition.

scholarly journals Population, and its social and religious composition in Ivanovo and Yaroslavl regions on the eve of the Axis aggression against the USSR (according to the censuses of the USSR in 1937 and 1939)

2021 ◽  
Vol 27 (1) ◽  
pp. 74-78
Author(s):  
Natalya S. Maiorova
Keyword(s):  
World War Ii ◽  
Rural Areas ◽  
Urban Population ◽  
Daily Life ◽  
World War ◽  
Religious Policy ◽  
The Core ◽  
Long Time ◽  
High Level ◽  
Anxiety And Fear

The article is devoted to the analysis of the results of population censuses conducted in the USSR in 1937 and 1939, in relation to Ivanovo and Yaroslavl regions. The research is based on census materials that had been classified for a long time and published only in the 1990s. Of all the various aspects of the censuses, the author's attention was focused on only three – population, its social structure, and religious composition. Based on the results of the censuses, conclusions are drawn about the prevalence of women in the region, both in rural areas and in cities. It was women who, in the conditions of World War II, became the strong rear, on whose shoulders the front was supported by food, uniforms, and weapons. The urban population was greater in Ivanovo Region, which was explained by its characteristic high rates of industrialisation. The 1937 census recorded a fairly high level of religiosity, despite the largely anti-religious policy that had been carried out for almost 20 years. The war led to an increase in religiosity, probably because often only faith could become the core around which daily life was built, full of deprivation, anxiety and fear for loved ones.

scholarly journals Machine Learning in Python: Main Developments and Technology Trends in Data Science, Machine Learning, and Artificial Intelligence

Information ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 193 ◽  
Author(s):  
Sebastian Raschka ◽  
Joshua Patterson ◽  
Corey Nolet
Keyword(s):  
Artificial Intelligence ◽  
Machine Learning ◽  
Data Science ◽  
Gpu Computing ◽  
Graphics Processing Unit ◽  
General Purpose ◽  
Processing Unit ◽  
The Core ◽  
Critical Components ◽  
High Level

Smarter applications are making better use of the insights gleaned from data, having an impact on every industry and research discipline. At the core of this revolution lies the tools and the methods that are driving it, from processing the massive piles of data generated each day to learning from and taking useful action. Deep neural networks, along with advancements in classical machine learning and scalable general-purpose graphics processing unit (GPU) computing, have become critical components of artificial intelligence, enabling many of these astounding breakthroughs and lowering the barrier to adoption. Python continues to be the most preferred language for scientific computing, data science, and machine learning, boosting both performance and productivity by enabling the use of low-level libraries and clean high-level APIs. This survey offers insight into the field of machine learning with Python, taking a tour through important topics to identify some of the core hardware and software paradigms that have enabled it. We cover widely-used libraries and concepts, collected together for holistic comparison, with the goal of educating the reader and driving the field of Python machine learning forward.

scholarly journals Coupling and Coordination Degrees of the Core Water–Energy–Food Nexus in China

2019 ◽  
Vol 16 (9) ◽  
pp. 1648 ◽  
Author(s):  
Shasha Xu ◽  
Weijun He ◽  
Juqin Shen ◽  
Dagmawi Mulugeta Degefu ◽  
Liang Yuan ◽  
...  
Keyword(s):  
Complex System ◽  
Sustainable Development ◽  
Mean Value ◽  
Key Factors ◽  
The Core ◽  
The Mean ◽  
Development Levels ◽  
High Level ◽  
Coordination Development ◽  
Coordination Degree

Achieving sustainable development in the water–energy–food (WEF) nexus is gaining global attention. The coupling and coordination degrees are a way to measure sustainable development levels of a complex system. This study assessed the coupling and coordination degrees of the core WEF nexus and identified key factors that affect sustainable development. First, an index system for assessing coupling and coordination degrees of the core WEF nexus was built. Second, the development levels of three subsystems as well as the coupling and coordination degrees of the core WEF nexus in China were calculated. The results showed that from 2007 to 2016, the mean value of the coupling degree was 0.746 (range (0.01, 1)), which was a high level. This proved that the three resources were interdependent. Hence, it was necessary to study their relationship. However, the mean value of the coordination degree was 0.395 (range (0, 1)), which was a low level. This showed that the coordination development of the core WEF nexus in China was low. It is necessary to take some measures to improve the situation. According to the key factors that affect the development levels of water, energy, and food subsystems, the authors put forward some suggestions to improve the coordination development of the WEF system in China.

scholarly journals Expression System for High Levels of GAG Lyase Gene Expression and Study of the hepA Upstream Region in Flavobacterium heparinum

2002 ◽  
Vol 184 (12) ◽  
pp. 3242-3252 ◽  
Author(s):  
Françoise Blain ◽  
A. Lydia Tkalec ◽  
Zhongqi Shao ◽  
Catherine Poulin ◽  
Marc Pedneault ◽  
...  
Keyword(s):  
Transcriptional Start Site ◽  
Expression System ◽  
Regulatory Region ◽  
Start Codon ◽  
Upstream Region ◽  
Wild Type ◽  
Heparinase I ◽  
High Level ◽  
Flavobacterium Heparinum ◽  
Transcriptional Start Sites

ABSTRACT A system for high-level expression of heparinase I, heparinase II, heparinase III, chondroitinase AC, and chondroitinase B in Flavobacterium heparinum is described. hepA, along with its regulatory region, as well as hepB, hepC, cslA, and cslB, cloned downstream of the hepA regulatory region, was integrated in the chromosome to yield stable transconjugant strains. The level of heparinase I and II expression from the transconjugant strains was approximately fivefold higher, while heparinase III expression was 10-fold higher than in wild-type F. heparinum grown in heparin-only medium. The chondroitinase AC and B transconjugant strains, grown in heparin-only medium, yielded 20- and 13-fold increases, respectively, in chondroitinase AC and B expression, compared to wild-type F. heparinum grown in chondroitin sulfate A-only medium. The hepA upstream region was also studied using cslA as a reporter gene, and the transcriptional start site was determined to be 26 bp upstream of the start codon in the chondroitinase AC transconjugant strain. The transcriptional start sites were determined for hepA in both the wild-type F. heparinum and heparinase I transconjugant strains and were shown to be the same as in the chondroitinase AC transconjugant strain. The five GAG lyases were purified from these transconjugant strains and shown to be identical to their wild-type counterparts.

Localization of distal regulatory domains in the megakaryocyte-specific platelet basic protein/platelet factor 4 gene locus

Blood ◽  
2001 ◽  
Vol 98 (3) ◽  
pp. 610-617 ◽  
Author(s):  
Chunyan Zhang ◽  
Michael A. Thornton ◽  
M. Anna Kowalska ◽  
Bruce S. Sachis ◽  
Michael Feldman ◽  
...  
Keyword(s):  
Gene Locus ◽  
Basic Protein ◽  
Regulatory Region ◽  
Platelet Factor 4 ◽  
Specific Gene ◽  
Sufficient Information ◽  
Specific Expression ◽  
Platelet Factor ◽  
High Level

Abstract The genes for the related human (h) chemokines, PBP (platelet basic protein) and PF4 (platelet factor 4), are within 5.3 kilobases (kb) of each other and form a megakaryocyte-specific gene locus. The hypothesis was considered that the PBP and PF4 genes share a common distal regulatory region(s) that leads to their high-level megakaryocyte-specific expression in vivo. This study examined PBP and PF4 expression in transgenic mice using 4 distinct humanPBP/PF4 gene locus constructs. These studies showed that within the region studied there was sufficient information to regulate tissue-specific expression of both hPBP and hPF4. Indeed this region contained sufficient DNA information to lead to expression levels of PBP and PF4 comparable to the homologous mouse genes in a position-independent, copy number–dependent fashion. These studies also indicated that the DNA domains that led to this expression were distinct for the 2 genes; hPBP expression is regulated by a region that is 1.5 to 4.4 kb upstream of that gene. Expression of hPF4 is regulated by a region that is either intergenic between the 2 genes or immediately downstream of the hPF4 gene. Comparison of the available human and mouse sequences shows conserved flanking region domains containing potential megakaryocyte-related transcriptional factor DNA-binding sites. Further analysis of these regulatory regions may identify enhancer domains involved in megakaryopoiesis that may be useful in the selective expression of other genes in megakaryocytes and platelets as a strategy for regulating hemostasis, thrombosis, and inflammation.

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