scholarly journals Comparative in vitro antioxidant and antibacterial activities of leaf extract fractions of Crimson bottlebrush, Callistemon citrinus (Curtis.) Skeels

2021 ◽  
Author(s):  
Poulomi Ghosh ◽  
Souren Goswami ◽  
Sujit Roy ◽  
Ria Das ◽  
Tista Chakraborty ◽  
...  
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Total Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Bacterial Strains ◽  
Total Antioxidant ◽  
Extract Fraction

AbstractObjectivesThe present study aimed to analyze a comparative in vitro free radical scavenging and antibacterial potentials of leaf aqueous and the successive extract fractions of Callistemon citrinus.MethodsFor in vitro antioxidant activity assessments, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, Fe3+ ion reducing antioxidant power (FRAP) assay, and total antioxidant capacity of the extracts were tested. Antibacterial potentials were tested through Agar well diffusion method using both gram-positive and gram-negative bacterial strains.ResultsData indicate the polar successive aqueous fraction (AQF) possesses the highest free radicals scavenging capacity, with lowest EC50 (the required extract concentration to scavenge half of the free radicals) for DPPH and FRAP assays, and contains the highest total phenolics (308.2±5.9 µg tannic acid equivalent/mg dry extract, DE), flavonoids (516.7±3.5 µg quercetin equivalent/mg DE), total antioxidant capacity (441.48±12.8 µg ascorbic acid equivalent/mg DE). Phenolics and flavonoids contents were positively correlated with the in vitro antioxidant activities. The antibacterial study indicates the petroleum ether and chloroform are suitable solvents for extracting antibacterial phytochemicals from C. citrinus leaves that are effective against both gram-positive and negative bacterial strains.ConclusionThe most polar fraction i.e. the successive aqueous extract fraction of C. citrinus leaves exhibited the highest antioxidant activities while the most non-polar petroleum ether extract fraction showed the highest antibacterial potentials thus these extract fractions might have therapeutic importance.

scholarly journals Extraction Optimization of Galla Turcica Polysaccharides and Determination of Its Antioxidant Activities In Vitro

2020 ◽  
Vol 15 (3) ◽  
pp. 1934578X2091176
Author(s):  
Jiangxia Hu ◽  
Jiayu Gao ◽  
Zijun Zhao ◽  
Xiao Yang ◽  
Lan Chen
Keyword(s):  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Protective Effects ◽  
Ultrasonic Time ◽  
Health Products ◽  
Total Antioxidant ◽  
Ultrasonic Assisted

Though natural polysaccharides commonly show antioxidant activities, the current research on the isolation of polysaccharides from Galla Turcica and their antioxidant activities still remain as an ongoing challenge. In this work, response surface analysis was employed to optimize an ultrasonic-assisted extraction method for polysaccharides of Galla Turcica. Their antioxidant and free radical scavenging activities were then evaluated using 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), total antioxidant activity, and iron ion reduction assays. Moreover, the protective effects of polysaccharides of Galla Turcica were determined on human embryonic kidney fibroblast 293 and hepatoma 7721 cells by cell proliferation assay. Overall, the key parameters of Galla Turcica polysaccharides extraction were optimized as crushing degree 100 mesh, ultrasonic time 50 min, and materials–liquid ratio 1:50. The isolated polysaccharides presented dose-dependent antioxidant and free radical scavenging effects in vitro. It also demonstrated an effective protective effect for human cells under oxidative damage. The results firstly determined the antioxidant activities of polysaccharides from Galla Turcica, thus providing a new natural resource for future investigation and development of the polysaccharides-based antioxidant drugs, health products, or additives.

scholarly journals In Vitro and In Vivo Antioxidant Properties of Taraxacum officinale in Nω-Nitro-l-Arginine Methyl Ester (L-NAME)-Induced Hypertensive Rats

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 309
Author(s):  
Olukayode O. Aremu ◽  
Adebola O. Oyedeji ◽  
Opeoluwa O. Oyedeji ◽  
Benedicta N. Nkeh-Chungag ◽  
Constance R. Sewani Rusike
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Methyl Ester ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Hypertensive Rats ◽  
Total Antioxidant

Oxidative stress has gained attention as one of the fundamental mechanisms responsible for the development of hypertension. The present study investigated in vitro and in vivo antioxidant effects of 70% ethanol-water (v/v) leaf and root extracts of T. officinale (TOL and TOR, respectively). Total phenolic and flavonoid content of plant extracts were assessed using Folin Ciocalteau and aluminium chloride colorimetric methods; while, 2,2-diphenyl-1-picrlhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) protocols were used to determine the free radical scavenging and total antioxidant capacities (TAC), respectively. The in vivo total antioxidant capacity and malondialdehyde acid (MDA) levels for lipid peroxidation tests were performed on organ homogenate samples from Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive rats treated with leaf extract, TOL (500 mg/kg/day) and TOR (500 mg/kg/day) for 21 days. Results showed that compared to TOR, TOL possessed significantly higher (p < 0.01) polyphenol (4.35 ± 0.15 compared to 1.14 ± 0.01) and flavonoid (23.17 ± 0.14 compared to 3 ± 0.05) content; free radical scavenging activity (EC50 0.37 compared to 1.34 mg/mL) and total antioxidant capacities (82.56% compared to 61.54% ABTS, and 156 ± 5.28 compared to 40 ± 0.31 FRAP) and both extracts showed no toxicity (LD50 > 5000 mg/kg). TOL and TOR significantly (p < 0.01) elevated TAC and reduced MDA levels in targets organs. In conclusion, T. officinale leaf extract possesses significant anti-oxidant effects which conferred significant in vivo antioxidant protection against free radical-mediated oxidative stress in L-NAME-induced hypertensive rats.

scholarly journals Domestic Cooking Effects of Bambara Groundnuts and Common Beans in the Antioxidant Properties and Polyphenol Profiles

2017 ◽  
Vol 6 (2) ◽  
pp. 24 ◽  
Author(s):  
V. Nyau ◽  
S. Prakash ◽  
J. Rodrigues ◽  
J. Farrant
Keyword(s):  
Free Radical ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Esi Ms ◽  
Positive Effects ◽  
Phytochemical Profiles

Processing of legumes before consumption has several effects on micronutrients, macronutrients and phytonutrients. This study was undertaken to investigate the effect of domestic processing on antioxidant activities and phenolic phytochemicals of the red bambara groundnuts and red beans. The study employed in vitro antioxidant assays (DPPH and FRAP) to screen for antioxidant properties, HPLC-PDA-ESI-MS and Folin Ciocalteu assay to screen for phenolic phytochemical profiles. Domestic cooking displayed positive effects on the antioxidant activity and phenolic phytochemical profiles of the two legumes. The free radical scavenging speed increased 10-fold in the methanolic extract from cooked red bambara groundnuts compared to uncooked. By contrast, the free radical scavenging speed increased 20-fold in the methanolic extract from cooked red beans compared to uncooked. HPLC-PDA-ESI-MS profiles of the cooked red bambara groundnuts and red beans revealed a number of emergent phenolic compounds, mainly flavonoids. These data indicate that cooking appear to enhance the nutraceutical profiles of the legumes investigated.

scholarly journals ANTIOXIDANT ACTIVITIES OF ROPINIROLE AND PRAMIPEXOLE NOVEL DRUGS USED IN TREATMENT OF PARKINSONISM: AN IN VITRO APPROACH

2016 ◽  
Vol 9 (9) ◽  
pp. 105 ◽  
Author(s):  
Preetha Selva ◽  
Srinivasan Vengadassalapathy
Keyword(s):  
Free Radical ◽  
Dopamine Agonist ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Novel Drugs ◽  
Dpph Method ◽  
Selective Dopamine ◽  
Free Radical Scavenging Activities

ABSTRACTObjective: The objective of this study is to evaluate and compare the free radical scavenging activities of selective dopamine agonist namely ropiniroleand pramipexole.Methods: The antioxidant activity of ropinirole and pramipexole at various concentrations was done by 1, 1-diphenyl-2picrylhydrazyl (DPPH) freeradical scavenging assay comparing it with ascorbic acid which was taken as standard.Results: The free radical scavenging property as measured by DPPH method showed that ropinirole and pramipexole have got a potent free radicalscavenging activity with that of ascorbic acid.Conclusion: Novel drugs such as pramipexole and ropinirole are promising molecules in the field of oxidative damage related neurodegenerativedisorders providing us an optimistic targeted approach toward neuroprotection.Keywords: Free radical scavenging, 1, 1-diphenyl-2picrylhydrazyl assay, Anti-Parkinson’s, Dopamine agonist.

scholarly journals In vitro and in vivo assessment of free radical scavenging and antioxidant activities of Veronica persica Poir

2018 ◽  
Vol 64 (8) ◽  
pp. 57 ◽  
Author(s):  
Javad Sharifi-Rad ◽  
Mehdi Sharifi-Rad ◽  
Bahare Salehi ◽  
Marcello Iriti ◽  
Amir Roointan ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
In Vivo Assessment

scholarly journals Evaluation of the Free Radical Scavenging Activities of Ellagic Acid and Ellagic Acid Peracetate by EPR Spectrometry

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4800
Author(s):  
Ajit Kumar ◽  
Preeti Kaushik ◽  
Sandra Incerpi ◽  
Jens Z. Pedersen ◽  
Sanjay Goel ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Free Radicals ◽  
Free Radical ◽  
Ellagic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Cellular Systems ◽  
Ros Production ◽  
Microsomal Lipid Peroxidation

The purpose of this study was to examine the free radical scavenging and antioxidant activities of ellagic acid (EA) and ellagic acid peracetate (EAPA) by measuring their reactions with the radicals, 2,2-diphenyl-1-picrylhydrazyl and galvinoxyl using EPR spectroscopy. We have also evaluated the influence of EA and EAPA on the ROS production in L-6 myoblasts and rat liver microsomal lipid peroxidation catalyzed by NADPH. The results obtained clearly indicated that EA has tremendous ability to scavenge free radicals, even at concentration of 1 µM. Interestingly even in the absence of esterase, EAPA, the acetylated product of EA, was also found to be a good scavenger but at a relatively slower rate. Kinetic studies revealed that both EA and EAPA have ability to scavenge free radicals at the concentrations of 1 µM over extended periods of time. In cellular systems, EA and EAPA were found to have similar potentials for the inhibition of ROS production in L-6 myoblasts and NADPH-dependent catalyzed microsomal lipid peroxidation.

Synthesis, Characterization, Free-radical Scavenging Capacity and Antioxidant Activity of Novel Series of Hydrazone, 1,3,4-oxadiazole and 1,2,4- triazole Derived from 3,5-dimethyl-1H-pyrazole

2019 ◽  
Vol 16 (7) ◽  
pp. 712-720 ◽  
Author(s):  
Khalid Karrouchi ◽  
Saad Fettach ◽  
Smaail Radi ◽  
El bekkaye Yousfi ◽  
Jamal Taoufik ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Cancer ◽  
Ic50 Values

Background: Pyrazole is an important class of heterocyclic compound, has been shown to exhibit diverse biological and pharmacological activities such as anti-inflammatory, anti-cancer, antioxidant, etc. Methods: In this study, a series of novel 3,5-dimethyl-1H-pyrazole derivatives containing hydrazine 4a-l have been synthesized via the reaction of the 2-(3,5-dimethyl-1H-pyrazol-1-yl)acetohydrazide. All synthesized compounds have been tested for their in vitro antioxidant activities via utilization of 1,1-biphenyl-2-picrylhydrazyl (DPPH) as a free radical scavenging reagent. Results: The data reported herein indicates that compound 4k showed potential radical scavenging capacity and compounds 4f and 4g exhibited best activity for the iron binding while comparing with positive controls. Conclusion: Good activity was noted for some compounds. In particular, compound 4k showed the highest antioxidant activity with IC50 values of 22.79 ± 3.64 and 1.35 ± 0.66 μg/mL in the DPPH and ABTS tests, respectively.

Nrf2/HO-1 Mediated Antioxidant Activities, Cytotoxicity Analysis and LCESI/ MS Profiling of Eulophia nuda L.

2020 ◽  
Vol 10 (1) ◽  
pp. 69-79 ◽  
Author(s):  
Vikas Nanekar ◽  
Varsha Shriram ◽  
Tushar Khare ◽  
Vinay Kumar
Keyword(s):  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Fold Increase ◽  
In Vitro Models ◽  
Free Radical Scavenging ◽  
Related Factor ◽  
Mcf7 Cells ◽  
Esi Ms

Background: Eulophia nuda, is a highly medicinal orchid with strong antioxidant and anticancer potentials in traditional systems of medicine. But few reports are available on the scientific validation. The aim of the study was to investigate phytochemical constituents, antioxidant and cytotoxic efficacies of extracts of Eulophia nuda, and the underlying mechanisms-of-action via upregulation of nuclear transcription factor-erythroid-2 related factor (Nrf2) and hemeoxygenase-1 (HO-1) pathways. Methods: Petroleum Ether (PEE), Ethyl Acetate (EAE), Methanol (ME) and Aqueous Methanol (AqME) extracts of shade dried tubers were obtained and concentrated in vacuo. Total phenols, flavonoids, condensed tannins, ascorbic acid and carotenoids were estimated from the extracts using standard methods. Antioxidant activities of extracts were determined by total antioxidant, FRAP, ABTS, DPPH, OH, H2O2, NO, O2 ·- radical scavenging assays. Cytotoxicity of EAE and ME were assessed against MCF7 cells in vitro. LC-ESI/MS profiling of EAE was carried out. Quantitative Real-Time (qRT) PCR was used for the expression analysis of Nrf2 and HO1 genes in EAE-treated MCF7 cells. Results: In vitro models confirmed strong dose-dependent antioxidant and free-radical scavenging potencies of E. nuda tuber extracts. Overall antioxidant efficacies were in the order EAE > ME > AqME > PEE. EAE showed striking cytotoxicity followed by ME (0.86% and 5.17% cell survival at 1000 µg ml-1, respectively). LC-ESI/MS profiling of most potent extract EAE revealed 37 identified compounds including catechin, taxifolin, tocopherol, trigallic acid and chlorogenic acid, all known for their strong antioxidant/anticancer properties. Expression levels of Nrf2 and HO1 genes were up-regulated in MCF7 cells beyond 50 μg ml-1 extract concentration with > 2-fold increase at 200 µg ml-1 EAE. Conclusion: The data demonstrated that E. nuda extracts possess strong free radical scavenging and antioxidant efficacies and the mechanism of action may be via inducing Nrf2 and HO-1.

scholarly journals IN VITRO SCREENING AND CORRELATION OF ETHANOLIC PEEL EXTRACTS OF PUNICA GRANATUM AND CITRUS SINENSIS WITH RESPECT TO ANTIOXIDANT AND ANTIRADICAL ACTIVITY

2020 ◽  
pp. 52-56
Author(s):  
AJIT V SONTAKKE ◽  
ANUP S HENDRE ◽  
SANGITA R PATIL
Keyword(s):  
Free Radical ◽  
Citrus Sinensis ◽  
Antiradical Activity ◽  
Total Phenolics ◽  
Radical Scavenging ◽  
Punica Granatum ◽  
Free Radical Scavenging ◽  
Total Antioxidant ◽  
Free Radical Scavenging Assay

Objective: The objective of this study is to evaluate the effect of the ethanolic peel extracts of Punica granatum and Citrus sinensis on total phenolics, total antioxidants, and radical scavenging activities using different in vitro models. Methods: Pomegranate and orange fruits at the maturity stage were manually peeled, washed, and air dried before extraction. Dried peels were grinded, weighed, and extracted with 95% ethanol and were used for in vitro analysis. Different concentrations of extracts for each peel were prepared. Total phenolics content, total antioxidant capacity, and free radical scavenging activities of each peel extract were estimated in the form of Folin–Ciocalteu reagent assay, phosphomolybdenum assay, hydroxyl free radical scavenging assay, and superoxide free radical scavenging assay, respectively. Results: The ethanolic peel extracts of P. granatum and C. sinensis showed significant increased total phenolic contents and total antioxidant activity. Furthermore, radical scavenging activity (hydroxyl and superoxide) was found to be significant in both ethanolic peel extracts. Conclusion: It can be concluded from the study that the ethanolic peel extracts of P. granatum and C. sinensis has potential antioxidant and antiradical activity.

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