scholarly journals MALDI-ToF Protein Profiling as Potential Rapid Diagnostic Platform for COVID-19

2021 ◽  
Author(s):  
Prajkta Chivte ◽  
Zane LaCasse ◽  
Ventaka Devesh Reddy Seethi ◽  
Pratool Bharti ◽  
Elizabeth R. Gaillard
Keyword(s):  
Mass Spectrometry ◽  
High Specificity ◽  
Diagnostic Methods ◽  
Protein Profiling ◽  
Rt Pcr ◽  
High Demand ◽  
Non Invasive ◽  
Maldi Tof ◽  
Potential Biomarker ◽  
High Consistency

More than a year after the COVID-19 pandemic has been declared, the need still exists for accurate, rapid, inexpensive and non-invasive diagnostic methods that yield high specificity towards the current and newly emerging SARS-CoV-2 strains. Several studies have since established saliva as a more amenable specimen type in early detection and viral load quantitation as compared to the nasopharyngeal swabs. Considering the limitations and high demand for COVID-19 testing, we have employed MALDI-ToF mass spectrometry for the analysis of 60 gargle samples from human donors and compared the spectra with their respective RT-PCR results. Several standards including isolated human serum immunoglobulins and controls such as pre-COVID-19 saliva and heat inactivated SARS-CoV-2 virus were simultaneously analyzed to provide a relative view of the saliva and viral proteome as they would appear in this works methodology. Five potential biomarker peaks were established that demonstrated high consistency with PCR positive samples. Overall, the agreement of these results with RT-PCR testing was no less than 90% for the studied cohort, which consisted of young and largely asymptomatic student athletes. Further investigation of the potential biomarker peaks is necessary, however, from a clinical standpoint, these results are promising for a rapid and inexpensive COVID-19 assay.

scholarly journals Superiority of MALDI-TOF Mass Spectrometry over Real-Time PCR for SARS-CoV-2 RNA Detection

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 730
Author(s):  
Magda Rybicka ◽  
Ewa Miłosz ◽  
Krzysztof Piotr Bielawski
Keyword(s):  
Mass Spectrometry ◽  
Gold Standard ◽  
Viral Genome ◽  
False Negative ◽  
Rt Pcr ◽  
Rna Detection ◽  
Maldi Tof ◽  
Negative Results ◽  
False Negative Results ◽  
Pcr Test

At present, the RT-PCR test remains the gold standard for early diagnosis of SARS-CoV-2. Nevertheless, there is growing evidence demonstrating that this technique may generate false-negative results. Here, we aimed to compare the new mass spectrometry-based assay MassARRAY® SARS-CoV-2 Panel with the RT-PCR diagnostic test approved for clinical use. The study group consisted of 168 suspected patients with symptoms of a respiratory infection. After simultaneous analysis by RT-PCR and mass spectrometry methods, we obtained discordant results for 17 samples (10.12%). Within fifteen samples officially reported as presumptive positive, 13 were positive according to the MS-based assay. Moreover, four samples reported by the officially approved RT-PCR as negative were positive in at least one MS assay. We have successfully demonstrated superior sensitivity of the MS-based assay in SARS-CoV-2 detection, showing that MALDI-TOF MS seems to be ideal for the detection as well as discrimination of mutations within the viral genome.

scholarly journals Advanced Techniques in Clinical Practice: Use of Lab-on-a-Chip Electrophoresis and Other Methods in Protein Profiling

2009 ◽  
Vol 28 (4) ◽  
pp. 274-278 ◽  
Author(s):  
Olgica Trenčevska ◽  
Vasko Aleksovski ◽  
Kiro Stojanoski
Keyword(s):  
Mass Spectrometry ◽  
Clinical Practice ◽  
Cystatin C ◽  
Biological Fluids ◽  
Neurological Diseases ◽  
Protein Profile ◽  
Lab On A Chip ◽  
Protein Profiling ◽  
Complete Analysis ◽  
Potential Biomarker

Advanced Techniques in Clinical Practice: Use of Lab-on-a-Chip Electrophoresis and Other Methods in Protein ProfilingProteins in clinical practice are analyzed as important parameters in the determination and treatment of different diseases. The scopes of the analyses are mainly concentrated in two levels - analyses of the complete protein profile, or determination of an isolated protein. In this work, despite of the use of conventional methods, mainly electrophoresis, new techniques have been implemented in protein analyses. Lab-on-a-chip is an electrophoretic technique that, when optimized, provides analyses of the total protein profile. When normal samples are compared to samples obtained from patients with different neurological diseases, characteristic patterns can be noted. Also, correlation and comparison can be made between the newly developed microchip electrophoresis method and the results obtained using the conventional techniques. When an analysis of a specific protein is necessary, mass spectrometry has proven to give best results, in both the se lectivity and specificity of analyses. It is believed that cystatin C is a potential biomarker in neurological diseases; therefore, the mass spectrometry method has been developed in order to obtain qualitative and quantitative analyses of biological fluids. Using the developed method of mass spectrometry immunoassay (MSIA), cystatin C was easily isolated and analyzed, obtaining complete analysis within minutes. The resulting mass spectra revealed various levels of cystatin C isoforms in serum and CSF samples.

scholarly journals Application of protein profiling of virulent Haemophilus parasuis by MALDI-TOF mass spectrometry

2016 ◽  
Vol 10 (06) ◽  
pp. 678-681 ◽  
Author(s):  
Luisa Z. Moreno ◽  
Givago F. R. Silva ◽  
Vasco T. M. Gomes ◽  
Carlos E. C. Matajira ◽  
Ana Paula S. Silva ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Full Text ◽  
Protein Profiling ◽  
Haemophilus Parasuis ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

This item has no abstract. Follow the links below to access the full text.

scholarly journals APPLICATION OF TIME-OF-FLIGHT MASS-SPECTROMETRY FOR DETECTION OF CAUSATIVE AGENT OF BRUCELLOSIS IN BLOOD SAMPLES IN EXPERIMENT

2017 ◽  
pp. 9-17
Author(s):  
D. V. Ulshina ◽  
D. A. Kovalev ◽  
D. G. Ponomarenko ◽  
D. V. Rusanova ◽  
N. M. Shvetsova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Causative Agent ◽  
Brucella Melitensis ◽  
Time Of Flight ◽  
Protein Profiling ◽  
Blood Samples ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Aim.Study the possibility to apply time-of-flight mass-spectrometry for detection of causative agent of brucellosis in blood. Materials and methods. Brucella strains: 5 Brucella melitensis and 21 Brucella abortus. Protein profiling in linear mode on MALD1-TOF mass-spectrometer Microflex «Bruker Daltonics». Results. Technique for disinfection and preparation of blood samples was modified and optimized for MALDI-TOF MS analysis. 120 representative protein profiles of sera extract were obtained that contain brucellosis causative agent. A resulting peak-list (super-spectrum) of the studied protein fraction of blood extract of a conditionally healthy human within the studied group was formed and analyzed. Conclusion. A scheme of brucella detection in blood samples by MALDI-TOF MS is proposed, based on detection of a complex of 15 genus-specific fragments. Signals on mass-spectra of extracts of leukocyte fraction of blood, artificially contaminated with brucellosis causative agents are characterized.

Intragenic expression profile in tissue and plasma for the detection of TMPRSS2 rearrangements associated with prostate cancer

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. 5162-5162
Author(s):  
H. R. Sanders ◽  
H. Li ◽  
K. Z. Qu ◽  
Z. J. Zhang ◽  
A. D. Sferruzza ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
High Specificity ◽  
Ffpe Tissue ◽  
Rt Pcr ◽  
Multiple Partner ◽  
Prostate Hyperplasia ◽  
Non Invasive ◽  
Normal Individuals ◽  
Plasma Rna ◽  
Benign Prostate

5162 Background: TMPRSS2 gene rearrangements have been reported in 40%-85% of prostate cancer (PCa) patients and have not been found in normal individuals or those with benign prostate hyperplasia (BPH). However, multiple partner genes, including ETS transcription genes, and breakpoints have been reported. We developed an assay based on TMPRSS2 5′ and 3′ intragenic differential expression (IDE) to potentially serve as a diagnostic or prognostic marker for PCa. Methods: We analyzed TMPRSS2 in FFPE tissue from 20 patients (9 PCa and 11 BPH) and plasma from 42 patients (32 PCa and 10 BPH). IDE was expressed as a ratio of 3′:5′ transcript levels which were determined by real-time RT-PCR using distinct primer/probe sets. A normal 3′:5′ ratio (≥30) was established by comparing nonmalignant cells to tumor cells from FFPE tissue. This cutoff was subsequently used to identify abnormal ratios in plasma specimens. Results: In FFPE tissue, 100% of PCa samples had a 3′:5′ratio <30 and 91% of BPH samples were ≥30 ( Table ). RNA in 48% of plasma samples passed our QC criteria for acceptability. The 3′:5′ ratios were <30 in 47% and ≥30 in 6.7% PCa plasma. Conclusions: By measuring IDE, we are not limited to screening for known TMPRSS2/ETS gene translocations. In tissue, this approach enabled us to identify patients with PCa vs. BPH with high specificity. Although work is needed to improve plasma RNA quality, IDE of plasma TMPRSS2 may be a useful non-invasive diagnostic or prognostic tool. [Table: see text] No significant financial relationships to disclose.

scholarly journals Human tear peptide/protein profiling study of ocular surface diseases by SPE-MALDI-TOF mass spectrometry analyses

2014 ◽  
Vol 3 ◽  
pp. 206-215 ◽  
Author(s):  
Nerea González ◽  
Ibon Iloro ◽  
Javier Soria ◽  
Juan A. Duran ◽  
Alaitz Santamaría ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ocular Surface ◽  
Protein Profiling ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Ocular Surface Diseases

scholarly journals RT‐PCR/MALDI‐TOF mass spectrometry‐based detection of SARS‐CoV‐2 in saliva specimens

2021 ◽  
Author(s):  
Matthew M. Hernandez ◽  
Radhika Banu ◽  
Paras Shrestha ◽  
Armi Patel ◽  
Feng Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Rt Pcr ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals MALDI-TOF Mass Spectroscopy Applications in Clinical Microbiology

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Maryam Alizadeh ◽  
Leila Yousefi ◽  
Farzaneh Pakdel ◽  
Reza Ghotaslou ◽  
Mohammad Ahangarzadeh Rezaee ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bacterial Infections ◽  
Bacterial Species ◽  
Diagnostic Methods ◽  
Ionization Mass ◽  
Two Dimensional Gel Electrophoresis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Comprehensive Information ◽  
Tof Ms

There is a range of proteomics methods to spot and analyze bacterial protein contents such as liquid chromatography-mass spectrometry (LC-MS), two-dimensional gel electrophoresis, and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS), which give comprehensive information about the microorganisms that may be helpful within the diagnosis and coverings of infections. Microorganism identification by mass spectrometry is predicted on identifying a characteristic spectrum of every species so matched with an outsized database within the instrument. MALDI-TOF MS is one of the diagnostic methods, which is a straightforward, quick, and precise technique, and is employed in microbial diagnostic laboratories these days and may replace other diagnostic methods. This method identifies various microorganisms such as bacteria, fungi, parasites, and viruses, which supply comprehensive information. One of the MALDI-TOF MS’s crucial applications is bacteriology, which helps identify bacterial species, identify toxins, and study bacterial antibiotic resistance. By knowing these cases, we will act more effectively against bacterial infections.

scholarly journals Heavy Chain Disease Reviewed on MALDI-TOF

2021 ◽  
Vol 156 (Supplement_1) ◽  
pp. S11-S11
Author(s):  
Radwa Ewaisha ◽  
Angela Dispenzieri ◽  
Maria Willrich ◽  
Mindy Kohlhagen ◽  
David Murray
Keyword(s):  
Mass Spectrometry ◽  
Heavy Chain ◽  
Light Chain ◽  
Diagnostic Methods ◽  
Serum Samples ◽  
Heavy Chains ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Disease Biology ◽  
Heavy Chain Disease

Abstract Heavy Chain Disease (HCD) is a group of rare B-cell proliferative disorders. Diagnosis depends on the detection of a truncated heavy chain with no associated light chain, often done by serum or urine immunofixation. This approach has been reported to have low specificity, since associated light chain bands are sometimes not visible and heavy chain bands can be mistaken for polyclonal bands. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) offers improved sensitivity in detecting monoclonal immunoglobulins. Truncation is thought to happen in the constant heavy chain (CH1) region, detected as a fragment of mass 27,000 Da on MALDI-TOF. Other mass patterns have not been reported in the literature. In this study, frozen serum samples from 8 heavy chain disease patients were analyzed by MALDI-TOF mass spectrometry. Spectra were reviewed on Mass-Fix software and visually inspected for monoclonal peaks. We detected two types of patterns for the IgG heavy chain disease. One pattern shows an IgG heavy chain with truncated mass (27,000 Da vs. 50,000 Da) and another in which the mass of the IgG heavy chain is greater than normal (65,000 Da). Follow-up work demonstrates that these are most likely dimers of truncated heavy chains. Thus, mass spectrometry-based immunofixation can provide new insights into heavy chain disease biology, mechanism, and progression, which are not identified by traditional diagnostic methods.

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