scholarly journals Perinatal maternal chronic exposure to dibutyl phthalate promotes visceral obesity in adult female offspring

2021 ◽  
Author(s):  
Kunyan Zhou ◽  
Ran Cheng ◽  
Meina Yang ◽  
Xiaoyang Shen ◽  
Xiaoyan Luo ◽  
...  
Keyword(s):  
Adult Female ◽  
Dibutyl Phthalate ◽  
Corn Oil ◽  
Visceral Obesity ◽  
Female Offspring ◽  
Perinatal Exposure ◽  
Fat Percentage ◽  
Protein Levels ◽  
Significant Difference ◽  
Visceral Adipose

IntroductionMaternal exposure to dibutyl phthalate (DBP) may result in glucolipid dysfunction in female offspring. However, the underlying mechanisms remain elusive. We hypothesized that chronic maternal DBP exposure could induce abnormal metabolism of glucolipid.Materials and methodsSprague-Dawley rats were intraperitoneally injected with different doses of DBP, estradiol, and corn oil from gestational day 7 until the end of lactation. The weights, visceral fat percentage, serum lipid, insulin and glucose, protein levels of PI3K signal pathway in muscle were detected in F1 female offspring.ResultsAlthough the birth weight of F1 female offspring was not different among groups, the weights were heavier in DBP groups from postnatal day 7 to adult (P<0.001). The visceral adipose percentage in adult female offspring was increased by perinatal exposure to DBP (P<0.001). Decreased serum levels of triglyceride (P<0.0001), fasting glucose (P=0.004), prolactin (P=0.006), HOMA-IR (P=0.014) were found in female offspring exposed to DBP, but no difference for fasting insulin, total cholesterol, adiponectin. Increased protein levels of p-AKT, but decreased PTEN and GPR30 were observed in muscle of female offspring in DBP group, but without significant difference. None difference was observed for the protein levels of PI3K, AKT, GLUT4, InsR and IRS-1.ConclusionMaternal perinatal exposure to DBP induced obesity and accumulation of visceral adipose tissue for the adult female offspring. Serum glucolipid and local signal transduction of PTEN/PI3K/AKT pathway in muscle were not adversely affected by perinatal exposure to DBP for adult female offspring.

scholarly journals Developmental exposure to DDT or DDE alters sympathetic innervation of brown adipose in adult female mice

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Annalise N. vonderEmbse ◽  
Sarah E. Elmore ◽  
Kyle B. Jackson ◽  
Beth A. Habecker ◽  
Katherine E. Manz ◽  
...  
Keyword(s):  
Adult Female ◽  
Sympathetic Neurons ◽  
Sympathetic Innervation ◽  
Female Offspring ◽  
Perinatal Exposure ◽  
Female Mice ◽  
Cl 316,243 ◽  
Brown Adipose ◽  
Increased Risk ◽  
Sympathetic Regulation

Abstract Background Exposure to the bioaccumulative pesticide dichlorodiphenyltrichloroethane (DDT) and its metabolite dichlorodiphenyldichloroethylene (DDE) has been associated with increased risk of insulin resistance and obesity in humans and experimental animals. These effects appear to be mediated by reduced brown adipose tissue (BAT) thermogenesis, which is regulated by the sympathetic nervous system. Although the neurotoxicity of DDT is well-established, whether DDT alters sympathetic innervation of BAT is unknown. We hypothesized that perinatal exposure to DDT or DDE promotes thermogenic dysfunction by interfering with sympathetic regulation of BAT thermogenesis. Methods Pregnant C57BL/6 J mice were administered environmentally relevant concentrations of DDTs (p,p’-DDT and o,p’-DDT) or DDE (p,p’-DDE), 1.7 mg/kg and 1.31 mg/kg, respectively, from gestational day 11.5 to postnatal day 5 by oral gavage, and longitudinal body temperature was recorded in male and female offspring. At 4 months of age, metabolic parameters were measured in female offspring via indirect calorimetry with or without the β3 adrenergic receptor agonist, CL 316,243. Immunohistochemical and neurochemical analyses of sympathetic neurons innervating BAT were evaluated. Results We observed persistent thermogenic impairment in adult female, but not male, mice perinatally exposed to DDTs or p,p’-DDE. Perinatal DDTs exposure significantly impaired metabolism in adult female mice, an effect rescued by treatment with CL 316,243 immediately prior to calorimetry experiments. Neither DDTs nor p,p’-DDE significantly altered BAT morphology or the concentrations of norepinephrine and its metabolite DHPG in the BAT of DDTs-exposed mice. However, quantitative immunohistochemistry revealed a 20% decrease in sympathetic axons innervating BAT in adult female mice perinatally exposed to DDTs, but not p,p’-DDE, and 48 and 43% fewer synapses in stellate ganglia of mice exposed to either DDTs or p,p’-DDE, respectively, compared to control. Conclusions These data demonstrate that perinatal exposure to DDTs or p,p’-DDE impairs thermogenesis by interfering with patterns of connectivity in sympathetic circuits that regulate BAT. Graphical abstract

scholarly journals Perinatal Exposure of Mice to the Pesticide DDT Impairs Energy Expenditure and Metabolism in Adult Female Offspring

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e103337 ◽  
Author(s):  
Michele La Merrill ◽  
Emma Karey ◽  
Erin Moshier ◽  
Claudia Lindtner ◽  
Michael R. La Frano ◽  
...  
Keyword(s):  
Energy Expenditure ◽  
Adult Female ◽  
Female Offspring ◽  
Perinatal Exposure

scholarly journals Effects of exogenous adiponectin supplementation in early pregnant PCOS mice on the metabolic syndrome of adult female offspring

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Meng Zuo ◽  
Guotao Liao ◽  
Wenqian Zhang ◽  
Dan Xu ◽  
Juan Lu ◽  
...  
Keyword(s):  
Adult Female ◽  
Early Pregnancy ◽  
Testosterone Level ◽  
Metabolic Disorders ◽  
Serum Testosterone ◽  
Female Offspring ◽  
Metabolic Phenotype ◽  
Serum Testosterone Level ◽  
The Metabolic Syndrome ◽  
Pcos Group

Abstract Objective PCOS is a heterogeneous endocrine disorder with both reproductive and metabolic abnormalities. At present, PCOS has been confirmed to have a certain genetic background. Compared with healthy women, the vast majority of PCOS patients have hyperandrogenemia, and this excessive androgen exposure during pregnancy may affect the development of female fetuses. The aim of the current study was to investigate the effect of adiponectin intervention during early pregnancy of obese mice with PCOS on the metabolic phenotype of adult female offspring. Methods After the PCOS model was established, C57BL/6J mice were divided into maternal-control, maternal-PCOS, and maternal-PCOS + APN groups. DHEA-induced PCOS mice were supplemented with adiponectin (10 mg/kg/day) in the early pregnancy in order to eliminate adverse hormone exposure and then traced for endocrine indicators in their adult female offspring, which were observed for metabolism syndrome or endocrine disturbance and exhibited the main effects of APN. To further explore the underlying mechanism, the relative expressions of phosphorylated AMPK, PI3K, and Akt were detected in the ovaries of offspring mice. Results The serum testosterone level of the maternal-PCOS + APN group in early pregnancy was significantly lower than that of the maternal-PCOS group (p < 0.01). The serum testosterone level in the offspring-PCOS + APN group was significantly lower than in the offspring-PCOS group (p <0.05), the diestrus time characterized by massive granulocyte aggregation in the estrus cycle was significantly shorter than in the offspring-PCOS group (p<0.05), and the phenotypes of PCOS-like reproductive disorders and metabolic disorders, such as obesity, insulin resistance, impaired glucose tolerance, and hyperlipidemia, were also significantly improved in the offspring-PCOS + APN group (p < 0.05). Compared with the control group, the expression levels of phosphorylated AMPK, PI3K, and Akt in the offspring-PCOS group were significantly decreased (p < 0.05), while those in the offspring-PCOS + APN group were significantly increased (p < 0.05). Conclusions APN intervention in early pregnancy significantly reduced the adverse effects of maternal obesity and high androgen levels during pregnancy on female offspring and corrected the PCOS-like endocrine phenotype and metabolic disorders of adult female offspring. This effect may be caused by the activation of the AMPK/PI3K-Akt signaling pathway in PCOS offspring mice.

scholarly journals Piceatannol Is Superior to Resveratrol at Suppressing Adipogenesis in Human Visceral Adipose-Derived Stem Cells

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 366
Author(s):  
In Sil Park ◽  
Youngjin Han ◽  
HyunA Jo ◽  
Ki Won Lee ◽  
Yong Sang Song
Keyword(s):  
Stem Cells ◽  
Metabolic Diseases ◽  
Binding Protein ◽  
Visceral Obesity ◽  
Peroxisome Proliferator ◽  
Adipose Derived Stem Cells ◽  
Fatty Acid Binding ◽  
Chemical Structures ◽  
Visceral Adipose ◽  
Adipogenic Effect

Resveratrol (3,4′,5-trans-trihydroxystilbene) and piceatannol (3,3′,4′,5-trans-tetraphydroxystilbene) are major stilbene compounds that are predominantly present in various natural foods, such as berries and fruits. Both phytochemical compounds are consumed as dietary supplements to prevent various metabolic diseases and for their anti-aging properties. Adipose-derived stem cells from human visceral adipose tissue (vASCs) are a useful in vitro model for evaluating their adipogenic effect. Treatment with resveratrol and piceatannol significantly inhibited lipid accumulation in vASCs. Their effective concentrations were 5, 10, and 20 μM for inhibiting adipogenesis of vASCs. Interestingly, despite the similar chemical structures of the two compounds, piceatannol showed a higher anti-adipogenic effect at 20 μM than resveratrol in vASCs. Moreover, the inhibitory capacity of lipid droplet generation was higher for piceatannol at 20 μM than that of resveratrol. Piceatannol significantly attenuated the expression level of adipogenic markers (e.g., CCAAT/enhanced binding protein α (C/EBPα), peroxisome proliferator-activated receptor γ (PPARγ), and adipocyte fatty acid binding protein (aP2)) compared to resveratrol at the mRNA and protein levels. These results suggest that piceatannol is a superior anti-adipogenic compound compared to resveratrol in the vASC model of visceral obesity.

Comparison of CrossFit Barbara and classic resistance trainings for the protection of strength performance during off-season in kickboxers

2020 ◽  
pp. 1-8
Author(s):  
Cebrail Gençoğlu ◽  
İlhan Şen
Keyword(s):  
Resistance Training ◽  
Body Mass ◽  
Body Fat ◽  
Bench Press ◽  
Body Fat Percentage ◽  
Fat Percentage ◽  
Strength Performance ◽  
Significant Difference ◽  
Before And After ◽  
Push Up

BACKGROUND: The inability of athletes to train or the decrease in the intensity and frequency of training may cause athletes to lose performance. Particularly in view of the current COVID-19 pandemic, maintaining strength outside the normal framework provides an advantage to athletes for the next competitions. OBJECTIVE: To compare the CrossFit Barbara which can be applied easily at home during the off-season or some situations such as the epidemic limitation to classic resistance training methods used to maintain the strength performance of national kickboxers. METHODS: Forty-three national kickboxers, CrossFit (CF, n= 22), and resistance training (RT, n= 21), participated in this study. While CF performed 20 pull-ups, 30 push-ups, 40 sit-ups, and 50 squat exercises, RT performed bench press, lat pull down, leg press, biceps curl, and triceps extension exercises twice per week for six weeks. Before and after the six weeks, the following variables were measured; body mass (BM) and body fat percentage (FP), VO2max, bench press (BP), squat (SQ), leg strength (LS), hand grip strength (HGS), pull-up, push-up and counter movement jump (CMJ). RESULTS: BP (p< 0.001, F= 41.125, ηp2= 0.501), SQ (p< 0.001, F= 26.604, ηp2= 0.394), LS (p< 0.001, F= 15.234, ηp2= 0.271), push-up (p< 0.001, F= 31.978, ηp2= 0.438) and pull-up (p< 0.001, F= 24.410, ηp2= 0.373) values changed significantly in group-time interaction between CF and RT groups, while there was no significant difference for the BM (p= 0.198, F= 1.715, ηp2= 0.040), Fat (p= 0.265, F= 1.279, ηp2= 0.030), HGS (p= 0.665, F= 0.190, ηp2= 0.005, CMJ (p= 0.054, F= 3.946, ηp2= 0.088) and VO2max (p=0.747, F= 0.106, ηp2= 0.003). Furthermore, according to the before and after study values, BP, SQ, LS, and CMJ decreased significantly (p< 0.05) while BM, FP, HGS, VO2max, pull-up and push-up variables did not in the CF (p> 0.05). In the RT, the pull-up and push-up variables decreased significantly (p< 0.05) while there was no significant difference for BP, SQ, LS, HGS, VO2max, body mass, body fat percentage and CMJ (p> 0.05). CONCLUSION: CF Barbara workout was more effective in maintaining strength endurance performances, and RT in maintaining maximum strength performances. According to the individual performance needs of athletes, reasonable training method can be used to prevent performance decrement in the strength domain.

scholarly journals Oxidative Stress Mediates the Fetal Programming of Hypertension by Glucocorticoids

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 531
Author(s):  
Jeremy Lamothe ◽  
Sandhya Khurana ◽  
Sujeenthar Tharmalingam ◽  
Chad Williamson ◽  
Collin J. Byrne ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Blood Pressure ◽  
Superoxide Dismutase ◽  
Fetal Programming ◽  
Cardiovascular Health ◽  
Phenylalanine Hydroxylase ◽  
Epigallocatechin Gallate ◽  
Female Offspring ◽  
Glutathione Peroxidase 1 ◽  
Protein Levels

The field of cardiovascular fetal programming has emphasized the importance of the uterine environment on postnatal cardiovascular health. Studies have linked increased fetal glucocorticoid exposure, either from exogenous sources (such as dexamethasone (Dex) injections), or from maternal stress, to the development of adult cardiovascular pathologies. Although the mechanisms are not fully understood, alterations in gene expression driven by altered oxidative stress and epigenetic pathways are implicated in glucocorticoid-mediated cardiovascular programming. Antioxidants, such as the naturally occurring polyphenol epigallocatechin gallate (EGCG), or the superoxide dismutase (SOD) 4-hydroxy-TEMPO (TEMPOL), have shown promise in the prevention of cardiovascular dysfunction and programming. This study investigated maternal antioxidant administration with EGCG or TEMPOL and their ability to attenuate the fetal programming of hypertension via Dex injections in WKY rats. Results from this study indicate that, while Dex-programming increased blood pressure in male and female adult offspring, administration of EGCG or TEMPOL via maternal drinking water attenuated Dex-programmed increases in blood pressure, as well as changes in adrenal mRNA and protein levels of catecholamine biosynthetic enzymes phenylalanine hydroxylase (PAH), tyrosine hydroxylase (TH), dopamine beta hydroxylase (DBH), and phenylethanolamine N-methyltransferase (PNMT), in a sex-specific manner. Furthermore, programmed male offspring displayed reduced antioxidant glutathione peroxidase 1 (Gpx1) expression, increased superoxide dismutase 1 (SOD1) and catalase (CAT) expression, and increased pro-oxidant NADPH oxidase activator 1 (Noxa1) expression in the adrenal glands. In addition, prenatal Dex exposure alters expression of epigenetic regulators histone deacetylase (HDAC) 1, 5, 6, 7, 11, in male and HDAC7 in female offspring. These results suggest that glucocorticoids may mediate the fetal programming of hypertension via alteration of epigenetic machinery and oxidative stress pathways.

MATERNAL EXPOSURE TO DDE MAY INCREASE WEIGHT AND BODY-MASS IN ADULT FEMALE OFFSPRING

Epidemiology ◽  
2004 ◽  
Vol 15 (4) ◽  
pp. S117-S118 ◽  
Author(s):  
Wilfried Karmaus ◽  
Ihuoma Eneli
Keyword(s):  
Body Mass ◽  
Adult Female ◽  
Female Offspring ◽  
Maternal Exposure

Long-term treadmill training ameliorates endothelium-dependent vasorelaxation mediated by insulin and insulin-like growth factor-1 in hypertension

2015 ◽  
Vol 119 (6) ◽  
pp. 663-669 ◽  
Author(s):  
Yi-Yuan Lin ◽  
Shin-Da Lee ◽  
Chia-Ting Su ◽  
Tsung-Lin Cheng ◽  
Ai-Lun Yang
Keyword(s):  
Growth Factor ◽  
Insulin Receptor ◽  
Treadmill Training ◽  
Control Group ◽  
Insulin Like Growth Factor ◽  
Hypertensive Rats ◽  
Protein Levels ◽  
Significant Difference ◽  
Nos Inhibitors

Dysfunction of insulin and insulin-like growth factor-1 (IGF-1) is associated with the pathophysiology of hypertension. The influence of long-term exercise on vascular dysfunction caused by hypertension remains unclear. We investigated whether long-term treadmill training improved insulin- and IGF-1-mediated vasorelaxation in hypertensive rats. Eight-week-old male spontaneously hypertensive rats (SHR) were randomly divided into sedentary and exercise (SHR-EX) groups. The SHR-EX group was trained on a treadmill for 60 min/day, 5 days/wk, for 8 wk. Wistar-Kyoto rats (WKY) were used as the normal control group. After training, aortic insulin- and IGF-1-mediated vasorelaxation was evaluated in organ baths. Additionally, the roles of phosphatidylinositol 3-kinase (PI3K), nitric oxide synthase (NOS), and aortic protein expression were examined in the three groups. Compared with sedentary SHR and WKY groups, insulin- and IGF-1-mediated vasorelaxation was significantly enhanced to a nearly normal level in the SHR-EX group. After endothelial denudation, blunted and comparable vasorelaxation was found among the three groups. Pretreatment with selective PI3K and NOS inhibitors attenuated insulin- and IGF-1-mediated vasorelaxation, and no significant difference was found among the three groups after the pretreatment. The aortic protein levels of the insulin receptor (IR), IGF-1 receptor (IGF-1R), insulin receptor substrate-1 (IRS-1), and endothelial NOS (eNOS) were also significantly increased in the SHR-EX group compared with the other two groups. These results suggested that treadmill training elicited the amelioration of endothelium-dependent insulin/IGF-1-mediated vasorelaxation partly via the increased activation of PI3K and NOS, as well as the enhancement of protein levels of IR, IGF-1R, IRS-1, and eNOS, in hypertension.

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