A large-volume sputum dry storage and transportation device for molecular and culture-based diagnosis of tuberculosis

Technologies for preservation of specimens in the absence of cold chains are essential for optimum utilization of existing laboratory services in the developing world. We present a prototype called specimen transportation tube (SPECTRA-tube) for the collection, exposure-free drying, ambient transportation, and liquid state recovery of large-volume (>1 mL) specimens. Specimens introduced into SPECTRA-tube are dried in glass fiber membranes, which are critical for efficient liquid-state sample recovery by rehydration and centrifugation. Mycobacterium smegmatis (Msm)-spiked mock sputum dried in native Standard 17 glass fiber was stable for molecular testing after 10-day storage at 45°C, and for culture testing after 10- and 5-day storage at 37°C and 45°C, respectively. Compatibility with human sputum storage was demonstrated by dry storing Mycobacterium bovis-spiked pooled human sputum in SPECTRA-tube for 5 days at room temperature followed by successful qPCR detection. By significantly increasing the volume of samples that can be transported in the dry state and enabling recovery of the entire sample in liquid state, SPECTRA-tube presents a potential universal solution for the preservation and transportation of liquid specimens.

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Incorporation of Wastes from Marble to Produce Brick Materials

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.174-177.135 ◽

2012 ◽

Vol 174-177 ◽

pp. 135-139

Author(s):

Qing Bo Tian ◽

Li Zong Chen ◽

Li Na Xu ◽

Yong Guang Fang

Keyword(s):

Compressive Strength ◽

Glass Fiber ◽

Room Temperature ◽

Wood Flour ◽

Raw Material ◽

Water Addition ◽

Water Curing

A brick material was prepared with marble wastes as main raw material by pressure forming and water-curing at room temperature. With the increases of the amounts of water additions, the compressive strength increased gradually and obtained a highest value of 34.8MPa in the sample of the ratio of cement: wastes=20:100 at 17.0% water addition, above which the strength had an adverse change and decreased. The addition of glass fiber had small effects on the strength, water absorbing rate and density comparing with that of wood flour. The strengths of sample had changed obviously with the increment of the forming pressures. However, the strength would fall because of the lamination caused by the recovering effects of the wood flour in the sample with replacement of the wood flour to marble wastes, as the forming pressures were higher than 5.0 MPa.

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Room-temperature fluorozirconate glass fiber laser in the violet (412 nm)

Optics Letters ◽

10.1364/ol.20.001474 ◽

1995 ◽

Vol 20 (13) ◽

pp. 1474 ◽

Cited By ~ 18

Author(s):

D. S. Funk ◽

J. W. Carlson ◽

J. G. Eden

Keyword(s):

Fiber Laser ◽

Glass Fiber ◽

Room Temperature ◽

Fluorozirconate Glass

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Photo-induced radical polarization and liquid-state dynamic nuclear polarization using fullerene nitroxide derivatives

Physical Chemistry Chemical Physics ◽

10.1039/c7cp06073d ◽

2017 ◽

Vol 19 (47) ◽

pp. 31823-31829 ◽

Cited By ~ 12

Author(s):

Guoquan Liu ◽

Shu-Hao Liou ◽

Nikolay Enkin ◽

Igor Tkach ◽

Marina Bennati

Keyword(s):

Laser Irradiation ◽

Spin Polarization ◽

Dynamic Nuclear Polarization ◽

Liquid State ◽

Room Temperature ◽

Nuclear Polarization ◽

Continuous Wave ◽

Continuous Wave Laser ◽

Wave Laser ◽

Nmr Signal Enhancement

Continuous wave laser irradiation of fullerene–nitroxide derivatives at room temperature leads to spin polarization and NMR signal enhancement of toluene solvent protons.

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Large volume liquid state scalar Overhauser dynamic nuclear polarization at high magnetic field

Physical Chemistry Chemical Physics ◽

10.1039/c9cp02997d ◽

2019 ◽

Vol 21 (38) ◽

pp. 21200-21204 ◽

Cited By ~ 3

Author(s):

Thierry Dubroca ◽

Sungsool Wi ◽

Johan van Tol ◽

Lucio Frydman ◽

Stephen Hill

Keyword(s):

Magnetic Field ◽

Nuclear Magnetic Resonance ◽

Magnetic Resonance ◽

Magnetic Fields ◽

High Magnetic Field ◽

Dynamic Nuclear Polarization ◽

Large Volume ◽

Liquid State ◽

Nuclear Polarization ◽

High Magnetic Fields

Dynamic Nuclear Polarization (DNP) can increase the sensitivity of Nuclear Magnetic Resonance (NMR), but it is challenging in the liquid state at high magnetic fields.

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New technique for solid-phase immunoassay: application to hepatitis B surface antigen.

Clinical Chemistry ◽

10.1093/clinchem/25.1.178 ◽

1979 ◽

Vol 25 (1) ◽

pp. 178-182 ◽

Cited By ~ 1

Author(s):

H Park

Keyword(s):

Hepatitis B ◽

Surface Antigen ◽

Large Volume ◽

Room Temperature ◽

Solid Phase ◽

Hepatitis B Surface Antigen ◽

New Technique ◽

Sandwich Assay ◽

Communication Device ◽

Flow Through

Abstract I describe a new technique for improving the sensitivity of the solid-phase "sandwich" assay, by using the through-passage receptacle in a novel flow-communication device. The technique allows a large volume of serum to flow through the antibody-coated receptacle repeatedly during the incubation and is thus termed the "flow-through large-volume incubation" method. Binding of 125I-labeled hepatitis B surface antigen to its corresponding antibody on a solid-phase by this method was more rapid and persistent than binding by the conventional method. When the method was applied to the first incubation of the sandwich assay, the test for the antigen was rendered four-, eight-, and 32-fold as sensitive as an accepted third-generation test for the antigen, by incubating 5-mL volumes of serum at (a) room temperature for 18 h, (b) 45 degrees C for 8 h, or (c) room temperature for seven days, respectively.

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CW room temperature upconversion lasing in Er3+-doped fluoride glass fiber

Journal of Non-Crystalline Solids ◽

10.1016/s0022-3093(05)80550-2 ◽

1992 ◽

Vol 143 ◽

pp. 40-45 ◽

Cited By ~ 45

Author(s):

Kazuyuki Hirao ◽

Shinichi Todoroki ◽

Naohiro Soga

Keyword(s):

Glass Fiber ◽

Room Temperature ◽

Fluoride Glass

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Characterization of Irradiation Crosslinked Polyamides for Solar Thermal Applications—Basic Thermo-Analytical and Mechanical Properties

Polymers ◽

10.3390/polym10090969 ◽

2018 ◽

Vol 10 (9) ◽

pp. 969 ◽

Cited By ~ 4

Author(s):

Patrick Bradler ◽

Joerg Fischer ◽

Gernot Wallner ◽

Reinhold Lang

Keyword(s):

Glass Fiber ◽

Room Temperature ◽

Fiber Content ◽

Solar Thermal ◽

Beam Irradiation ◽

Lower Melting ◽

Short Glass Fiber ◽

Glass Fiber Reinforced ◽

Solar Thermal Applications ◽

Short Glass

Polyamide 66 (PA 66) and short glass fiber reinforced versions of PA 66 are widely used for solar-thermal applications, in which thermal and environmental loading of components is from high importance. In this study, the influence of crosslinking via electronic beam irradiation on the morphology and mechanical behavior of unreinforced PA 66 and two types of short glass fiber reinforced PA 66 (30 wt % glass fiber content, 35 wt % glass fiber content) was investigated. In total, five different electronic beam irradiation doses in the range of 0 and 200 kGy were applied. Besides experiments with unconditioned specimens, also preconditioned specimens saturated with water at 80 °C for seven days were investigated. It was found that irradiation causes a shift to lower melting temperatures and lower melting enthalpies, while simultaneously leading to higher glass transition temperatures (TG), increasing small strain modulus values and higher tensile strengths. Also, as expected, preconditioning samples in water at 80 °C to water uptake saturation leads to a shift to lower TG values (‘plasticization’ effect). In terms of tensile behavior at room temperature, water saturated specimens (being above TG at room temperature) exhibited lower modulus and tensile strength values compared to quasi-dry specimens (being below TG at room temperature).

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Interface Failure of Heated GLARETM Fiber–Metal Laminates under Bird Strike

Aerospace ◽

10.3390/aerospace7030028 ◽

2020 ◽

Vol 7 (3) ◽

pp. 28

Author(s):

Md.Zahid Hasan

Keyword(s):

Glass Fiber ◽

Room Temperature ◽

Elevated Temperatures ◽

Impact Damage ◽

Strain Energy Release ◽

Fiber Reinforced ◽

Interface Failure ◽

Glass Fiber Reinforced ◽

Cohesive Interfaces ◽

The Impact

Many high-strength composite materials have been developed for aircraft structures. GLAss fiber REinforced aluminum (GLARE) is one of the high-performance composites. The review of articles, however, yielded no study on the impact damage of heated GLARE laminates. This study, therefore, aimed at developing a numerical model that can delineate the continuum damage of GLARE 5A-3/2-0.3 laminates at elevated temperatures. In the first stage, the inter-laminar interface failure of heated GLARE laminate had been investigated at room temperature and 80 °C. The numerical analysis employed a three-dimensional GLARE 5A-3/2-0.3 model that accommodated volumetric cohesive interfaces between mating material layers. Lagrangian smoothed particles populated the projectile. The model considered the degradation of tensile and shear modulus of glass fiber reinforced epoxy (GF/EP) at 80 °C, while incorporated temperature-dependent critical strain energy release rate of cohesive interfaces. When coupled with the material particulars, an 82 m/s bird impact at room temperature exhibited delamination first in the GF/EP 90°/0° interface farthest from the impacted side. Keeping the impact velocity, interface failure propagated at a slower rate at 80 °C than that at room temperature, which was in agreement with the impact damage determined in the experiments. The outcomes of this study will help optimize a GLARE laminate based on the anti-icing temperature of aircraft.

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