Bioorthogonal Functionalization of Material Surfaces with Bioactive Molecules

The ability to control and modify the bioactivity of surfaces is pivotal to the success of many medical devices. A biocompatible and bioorthogonal method to functionalize surfaces with a wide variety of bioactive molecules is an important tool for enabling innovative biotechnology and medical applications. We report herein a novel, catecholamine-based surface functionalization method that is chemoselective and free of a metal catalyst. This method utilizes the ligation between a coating formed from the tyrosinase-catalyzed polymerization of a tetrazine-containing catecholamine and one or more strained alkene-containing molecules of interest. The process is achieved under conditions ideal for biological applications. Using this method, we graft surfaces with a variety of active molecules, including a small molecule fluorophore, enzymes, and a cyclic peptide with the RGD motif, and demonstrate the maintenance of their activity on the surface. Additionally, we establish the cytocompatibility of grafted ECM-mimicking surfaces with fibroblasts and show improved cell adhesion and cytoskeletal organization.

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Combinational Effect of Cell Adhesion Biomolecules and Their Immobilized Polymer Property to Enhance Cell-Selective Adhesion

International Journal of Polymer Science ◽

10.1155/2016/2090985 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 3

Author(s):

Rio Kurimoto ◽

Kei Kanie ◽

Naokazu Idota ◽

Mitsuo Hara ◽

Shusaku Nagano ◽

...

Keyword(s):

Cell Adhesion ◽

Medical Devices ◽

Molecular Conformation ◽

Cell Types ◽

Surface Hydrophobicity ◽

Bioactive Molecules ◽

Surface Immobilization ◽

Medical Implants ◽

Polymeric Substrate ◽

Glass Surfaces

Although surface immobilization of medical devices with bioactive molecules is one of the most widely used strategies to improve biocompatibility, the physicochemical properties of the biomaterials significantly impact the activity of the immobilized molecules. Herein we investigate the combinational effects of cell-selective biomolecules and the hydrophobicity/hydrophilicity of the polymeric substrate on selective adhesion of endothelial cells (ECs), fibroblasts (FBs), and smooth muscle cells (SMCs). To control the polymeric substrate, biomolecules are immobilized on thermoresponsive poly(N-isopropylacrylamide-co-2-carboxyisopropylacrylamide) (poly(NIPAAm-co-CIPAAm))-grafted glass surfaces. By switching the molecular conformation of the biomolecule-immobilized polymers, the cell-selective adhesion performances are evaluated. In case of RGDS (Arg-Gly-Asp-Ser) peptide-immobilized surfaces, all cell types adhere well regardless of the surface hydrophobicity. On the other hand, a tri-Arg-immobilized surface exhibits FB-selectivity when the surface is hydrophilic. Additionally, a tri-Ile-immobilized surface exhibits EC-selective cell adhesion when the surface is hydrophobic. We believe that the proposed concept, which is used to investigate the biomolecule-immobilized surface combination, is important to produce new biomaterials, which are highly demanded for medical implants and tissue engineering.

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Continuous Based Direct Ink Write for Tubular Cardiovascular Medical Devices

Polymers ◽

10.3390/polym13010077 ◽

2020 ◽

Vol 13 (1) ◽

pp. 77

Author(s):

Enric Casanova-Batlle ◽

Antonio J. Guerra ◽

Joaquim Ciurana

Keyword(s):

Medical Devices ◽

New Technology ◽

Vascular Grafts ◽

Mechanical Resistance ◽

Medical Applications ◽

End User ◽

Cardiovascular Stents ◽

Direct Ink Writing ◽

Late Restenosis ◽

Printing Parameters

Bioresorbable cardiovascular applications are increasing in demand as fixed medical devices cause episodes of late restenosis. The autologous treatment is, so far, the gold standard for vascular grafts due to the similarities to the replaced tissue. Thus, the possibility of customizing each application to its end user is ideal for treating pathologies within a dynamic system that receives constant stimuli, such as the cardiovascular system. Direct Ink Writing (DIW) is increasingly utilized for biomedical purposes because it can create composite bioinks by combining polymers and materials from other domains to create DIW-printable materials that provide characteristics of interest, such as anticoagulation, mechanical resistance, or radiopacity. In addition, bioinks can be tailored to encounter the optimal rheological properties for the DIW purpose. This review delves into a novel emerging field of cardiovascular medical applications, where this technology is applied in the tubular 3D printing approach. Cardiovascular stents and vascular grafts manufactured with this new technology are reviewed. The advantages and limitations of blending inks with cells, composite materials, or drugs are highlighted. Furthermore, the printing parameters and the different possibilities of designing these medical applications have been explored.

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Structural and ICAM-1-Docking Properties of a Cyclic Peptide from the I-domain of LFA-1: An inhibitor of ICAM-1/LFA-1-mediated T-cell adhesion

Journal of Biomolecular Structure and Dynamics ◽

10.1080/07391102.2002.10506785 ◽

2002 ◽

Vol 19 (5) ◽

pp. 789-799 ◽

Cited By ~ 10

Author(s):

Christine R. Xu ◽

Helena Yusuf-Makagiansar ◽

Yongbo Hu ◽

Seetharama D.S. Jois ◽

Teruna J. Siahaan

Keyword(s):

Cell Adhesion ◽

T Cell ◽

Cyclic Peptide ◽

T Cell Adhesion

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Directed adenine functionalization for creating complex architectures for material and biological applications

Chemical Communications ◽

10.1039/c7cc00222j ◽

2017 ◽

Vol 53 (35) ◽

pp. 4748-4758 ◽

Cited By ~ 24

Author(s):

Balaram Mohapatra ◽

Pratibha Pratibha ◽

Sandeep Verma

Keyword(s):

Metal Complexes ◽

Coordination Polymers ◽

Gas Adsorption ◽

Bioactive Molecules ◽

Biological Applications ◽

Design Strategies ◽

Feature Article ◽

Complex Architectures ◽

Adenine Derivatives

This feature article outlines design strategies for modified adenine derivatives to construct discrete metal complexes, ring-expanded skeletons, coordination polymers, MOFs, and capped nanoparticles, for applications in gas adsorption, as bioimaging agents and as bioactive molecules.

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Applications of Micro- and Nano-technology to Study Cell Adhesion to Material Surfaces

Surface and Interfacial Aspects of Cell Adhesion ◽

10.1201/b12179-11 ◽

2011 ◽

pp. 153-166 ◽

Cited By ~ 1

Keyword(s):

Cell Adhesion ◽

Nano Technology ◽

Material Surfaces

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Microsystems in Medicine

The International Journal of Artificial Organs ◽

10.1177/039139889802100304 ◽

1998 ◽

Vol 21 (3) ◽

pp. 137-146 ◽

Cited By ~ 12

Author(s):

U. Wallrabe ◽

P. Ruther ◽

T. Schaller ◽

W. K. Schomburg

Keyword(s):

Cell Culture ◽

Medical Devices ◽

Optical Sensors ◽

Salt Solution ◽

Medical Applications ◽

3 Dimensional ◽

Batch Fabrication ◽

Cell Culture Systems ◽

Dimensional Growth ◽

Plastic Containers

The complexity of modern surgical and analytical methods requires the miniaturisation of many medical devices. The LIGA technique and also mechanical microengineering are well known for the batch fabrication of microsystems. Actuators and sensors are developed based on these techniques. The hydraulic actuation principle is advantageous for medical applications since the energy may be supplied by pressurised balanced salt solution. Some examples are turbines, pumps and valves. In addition, optical sensors and components are useful for analysis and inspection as represented by microspectrometers and spherical lenses. Finally, plastic containers with microporous bottoms allow a 3-dimensional growth of cell culture systems.

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Regulation of the formation of osteoclastic actin rings by proline-rich tyrosine kinase 2 interacting with gelsolin

The Journal of Cell Biology ◽

10.1083/jcb.200207036 ◽

2003 ◽

Vol 160 (4) ◽

pp. 565-575 ◽

Cited By ~ 71

Author(s):

Qiang Wang ◽

Yi Xie ◽

Quan-Sheng Du ◽

Xiao-Jun Wu ◽

Xu Feng ◽

...

Keyword(s):

Cell Adhesion ◽

Tyrosine Kinase ◽

Actin Polymerization ◽

Actin Binding ◽

Cell Periphery ◽

Cytoskeletal Organization ◽

Capping Protein ◽

Tyrosine Kinase 2 ◽

Multiple Cells ◽

Actin Rings

Osteoclast activation is important for bone remodeling and is altered in multiple bone disorders. This process requires cell adhesion and extensive actin cytoskeletal reorganization. Proline-rich tyrosine kinase 2 (PYK2), a major cell adhesion–activated tyrosine kinase in osteoclasts, plays an important role in regulating this event. The mechanisms by which PYK2 regulates actin cytoskeletal organization and osteoclastic activation remain largely unknown. In this paper, we provide evidence that PYK2 directly interacts with gelsolin, an actin binding, severing, and capping protein essential for osteoclastic actin cytoskeletal organization. The interaction is mediated via the focal adhesion–targeting domain of PYK2 and an LD motif in gelsolin's COOH terminus. PYK2 phosphorylates gelsolin at tyrosine residues and regulates gelsolin bioactivity, including decreasing gelsolin binding to actin monomer and increasing gelsolin binding to phosphatidylinositol lipids. In addition, PYK2 increases actin polymerization at the fibroblastic cell periphery. Finally, PYK2 interacts with gelsolin in osteoclasts, where PYK2 activation is required for the formation of actin rings. Together, our results suggest that PYK2 is a regulator of gelsolin, revealing a novel PYK2–gelsolin pathway in regulating actin cytoskeletal organization in multiple cells, including osteoclasts.

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Tailored multifunctional micellar brushes via crystallization-driven growth from a surface

Science ◽

10.1126/science.aax9075 ◽

2019 ◽

Vol 366 (6469) ◽

pp. 1095-1098 ◽

Cited By ~ 21

Author(s):

Jiandong Cai ◽

Chen Li ◽

Na Kong ◽

Yi Lu ◽

Geyu Lin ◽

...

Keyword(s):

Gold Nanoparticles ◽

Graphene Oxide ◽

Surface Functionalization ◽

Two Dimensional ◽

Graphene Oxide Nanosheets ◽

Seeded Growth ◽

Material Surfaces ◽

Oil In Water ◽

Two Dimensional Materials ◽

Antibacterial Surface

The creation of nanostructures with precise chemistries on material surfaces is of importance in a wide variety of areas such as lithography, superhydrophobicity, and cell adhesion. We describe a platform for surface functionalization that involves the fabrication of cylindrical micellar brushes on a silicon wafer through seeded growth of crystallizable block copolymers at the termini of immobilized, surface-confined crystallite seeds. The density, length, and coronal chemistry of the micellar brushes can be precisely tuned, and post-growth decoration with nanoparticles enables applications in catalysis and antibacterial surface modification. The micellar brushes can also be grown on ultrathin two-dimensional materials such as graphene oxide nanosheets and further assembled into a membrane for the separation of oil-in-water emulsions and gold nanoparticles.

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