scholarly journals Simultaneous 3D super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for cell imaging

2019 ◽  
Author(s):  
Ana I. Gómez-Varela ◽  
Dimitar R. Stamov ◽  
Adelaide Miranda ◽  
Rosana Alves ◽  
Cláudia Barata-Antunes ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Fluorescence Microscopy ◽  
System Integration ◽  
Super Resolution ◽  
Resolution Limit ◽  
Force Microscopy ◽  
Spatially Correlated ◽  
Atomic Force ◽  
Set Up ◽  
Cell Variation

AbstractCorrelating data from different microscopy techniques holds the potential to discover new facets of signaling events in cellular biology. Here we report for the first time a hardware set-up capable of achieving simultaneous imaging of spatially correlated super-resolution fluorescence microscopy and atomic force microscopy, a feat only obtained until now by fluorescence microscopy set-ups with spatial resolution restricted to the Abbe resolution limit. We hereby remove the need to perform independent measurement and subsequent data averaging required to eliminate cell-to-cell variation in observed signals. We detail system integration, demonstrate system performance and report imaging of sub-resolution fluorescent beads and genome-engineered human bone osteosarcoma epithelial cells.

scholarly journals Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ana I. Gómez-Varela ◽  
Dimitar R. Stamov ◽  
Adelaide Miranda ◽  
Rosana Alves ◽  
Cláudia Barata-Antunes ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Fluorescence Microscopy ◽  
Life Sciences ◽  
Super Resolution ◽  
Force Microscopy ◽  
Atomic Force

Ultrasonically synthesized organic liquid-filled chitosan microcapsules: part 2: characterization using AFM (atomic force microscopy) and combined AFM–confocal laser scanning fluorescence microscopy

Soft Matter ◽  
2018 ◽  
Vol 14 (16) ◽  
pp. 3192-3201 ◽  
Author(s):  
Srinivas Mettu ◽  
Qianyu Ye ◽  
Meifang Zhou ◽  
Raymond Dagastine ◽  
Muthupandian Ashokkumar
Keyword(s):  
Atomic Force Microscopy ◽  
Fluorescence Microscopy ◽  
Young’S Modulus ◽  
Laser Scanning ◽  
Young's Modulus ◽  
Organic Liquid ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Force Microscopy ◽  
Atomic Force

Atomic Force Microscopy (AFM) is used to measure the stiffness and Young's modulus of individual microcapsules that have a chitosan cross-linked shell encapsulating tetradecane.

scholarly journals Recent Applications of Advanced Atomic Force Microscopy in Polymer Science: A Review

Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1142 ◽  
Author(s):  
Phuong Nguyen-Tri ◽  
Payman Ghassemi ◽  
Pascal Carriere ◽  
Sonil Nanda ◽  
Aymen Amine Assadi ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Chemical Characterization ◽  
Super Resolution ◽  
Polymeric Materials ◽  
Polymer Materials ◽  
Polymer Science ◽  
Force Microscopy ◽  
Atomic Force ◽  
Nanoscale Characterization

Atomic force microscopy (AFM) has been extensively used for the nanoscale characterization of polymeric materials. The coupling of AFM with infrared spectroscope (AFM-IR) provides another advantage to the chemical analyses and thus helps to shed light upon the study of polymers. This paper reviews some recent progress in the application of AFM and AFM-IR in polymer science. We describe the principle of AFM-IR and the recent improvements to enhance its resolution. We also discuss the latest progress in the use of AFM-IR as a super-resolution correlated scanned-probe infrared spectroscopy for the chemical characterization of polymer materials dealing with polymer composites, polymer blends, multilayers, and biopolymers. To highlight the advantages of AFM-IR, we report several results in studying the crystallization of both miscible and immiscible blends as well as polymer aging. Finally, we demonstrate how this novel technique can be used to determine phase separation, spherulitic structure, and crystallization mechanisms at nanoscales, which has never been achieved before. The review also discusses future trends in the use of AFM-IR in polymer materials, especially in polymer thin film investigation.

A high-speed atomic force microscopy with super resolution based on path planning scanning

2020 ◽  
Vol 213 ◽  
pp. 112991
Author(s):  
Yinan Wu ◽  
Yongchun Fang ◽  
Chao Wang ◽  
Cunhuan Liu ◽  
Zhi Fan
Keyword(s):  
Atomic Force Microscopy ◽  
Path Planning ◽  
High Speed ◽  
Super Resolution ◽  
Force Microscopy ◽  
Atomic Force

Fabrication of 2D-protein arrays using biotinylated thiols: results from fluorescence microscopy and atomic force microscopy

2004 ◽  
Vol 6 (17) ◽  
pp. 4358-4362 ◽  
Author(s):  
Christian Grunwald ◽  
Wolfgang Eck ◽  
Norbert Opitz ◽  
Jürgen Kuhlmann ◽  
Christof Wöll
Keyword(s):  
Atomic Force Microscopy ◽  
Fluorescence Microscopy ◽  
Protein Arrays ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals How did correlative atomic force microscopy and super-resolution microscopy evolve in the quest for unravelling enigmas in biology?

Nanoscale ◽  
2021 ◽  
Author(s):  
Adelaide Miranda ◽  
Ana I. Gómez-Varela ◽  
Andreas Stylianou ◽  
Liisa M. Hirvonen ◽  
Humberto Sánchez ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Super Resolution ◽  
Force Microscopy ◽  
Atomic Force ◽  
Microscopy Techniques ◽  
Super Resolution Microscopy ◽  
Detailed Picture

This review provides a detailed picture of the innovative efforts to combine atomic force microscopy and different super-resolution microscopy techniques to elucidate biological questions.

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