scholarly journals In silico stress fibre content affects peak strain in cytoplasm and nucleus but not in membrane for uniaxial substrate stretch

2019 ◽  
Author(s):  
Tamer Abdalrahman ◽  
Neil H. Davies ◽  
Thomas Franz
Keyword(s):  
Elastic Modulus ◽  
In Silico ◽  
Focal Adhesions ◽  
Element Model ◽  
Homogenization Method ◽  
Fibre Volume ◽  
Fibre Content ◽  
Peak Strain ◽  
Stress Fibre ◽  
A Cell

AbstractExisting in silico models for single cell mechanics feature limited representations of cytoskeletal structures that contribute substantially to the mechanics of a cell. We propose a micromechanical hierarchical approach to capture the mechanical contribution of actin stress fibres. For a cell-specific fibroblast geometry with membrane, cytoplasm and nucleus, the Mori-Tanaka homogenization method was employed to describe cytoplasmic inhomogeneities and constitutive contribution of actin stress fibres. The homogenization was implemented in a finite element model of the fibroblast attached to a substrate through focal adhesions. Strain in cell membrane, cytoplasm and nucleus due to uniaxial substrate stretch was assessed for different stress fibre volume fractions and different elastic modulus of the substrate. A considerable decrease of the peak strain with increasing stress fibre content was observed in cytoplasm and nucleus but not the membrane, whereas the peak strain in cytoplasm, nucleus and membrane increased for increasing elastic modulus of the substrate.

Effect of fibre content on the mechanical properties of hemp fibre woven fabrics/polypropylene composite laminates

2021 ◽  
pp. 096739112110239
Author(s):  
Sheedev Antony ◽  
Abel Cherouat ◽  
Guillaume Montay
Keyword(s):  
Mechanical Properties ◽  
Polymer Matrix ◽  
Composite Laminates ◽  
Volume Fraction ◽  
Fibre Volume Fraction ◽  
Fibre Volume ◽  
Woven Fabrics ◽  
Fibre Content ◽  
Viscoelastic Behaviour ◽  
Hemp Fibre

Nowadays natural fibre composites have gained great significance as reinforcements in polymer matrix composites. Composite material based on a polymer matrix reinforced with natural fibres is extensively used in industry due to their biodegradability, recyclability, low density and high specific properties. A study has been carried out here to investigate the fibre volume fraction effect of hemp fibre woven fabrics/PolyPropylene (PP) composite laminates on the tensile properties and impact hammer impact test. Initially, composite sheets were fabricated by the thermal-compression process with desired number of fabric layers to obtain composite laminates with different fibre volume fraction. Uniaxial, shear and biaxial tensile tests were performed and mechanical properties were calculated. Impact hammer test was also carried out to estimate the frequency and damping parameters of stratified composite plates. Scanning Electron Microscope (SEM) analysis was performed to observe the matrix and fibre constituent defects. Hemp fabrics/PP composite laminates exhibits viscoelastic behaviour and as the fibre volume fraction increases, the viscoelastic behaviour decreases to elastic behaviour. Due to this, the tensile strength increases as the fibre content increases. On the other hand, the natural frequency increases and damping ratio decrease as the fibre volume fraction increases.

In silico CDM model sheds light on force transmission in cell from focal adhesions to nucleus

2016 ◽  
Vol 49 (13) ◽  
pp. 2625-2634 ◽  
Author(s):  
Jean-Louis Milan ◽  
Ian Manifacier ◽  
Kevin M. Beussman ◽  
Sangyoon J. Han ◽  
Nathan J. Sniadecki ◽  
...  
Keyword(s):  
In Silico ◽  
Focal Adhesions ◽  
Force Transmission

Analysis of Packer Rubber Mechanics Properties

2014 ◽  
Vol 971-973 ◽  
pp. 380-389
Author(s):  
Jian Ning Wang ◽  
Gang Wu ◽  
Wei Yi Xie ◽  
Xin De Han ◽  
Ming Chao Gang
Keyword(s):  
Finite Element ◽  
Elastic Modulus ◽  
Constitutive Model ◽  
Contact Stress ◽  
Theoretical Basis ◽  
Element Model ◽  
Rubber Material ◽  
Bottom Hole ◽  
Sealing Performance ◽  
The Finite Element Model

Abstract: The packer rubber stress in the bottom hole is more complex. Based on constitutive model of the packer rubber material, this paper determines such parameters as model constants, Poisson's ratio of rubber materials and elastic modulus by using experimental method, to build up the finite element model of center tube-rubber cylinder-casing for the purpose of stress analysis. Finally, the distribution regularity of rubber cylinder-casing contact stress and packer setting travel distance with varying loads is concluded. The results can provide the theoretical basis for further analysis of packer rubber sealing performance.

Syndesmos, a protein that interacts with the cytoplasmic domain of syndecan-4, mediates cell spreading and actin cytoskeletal organization

2000 ◽  
Vol 113 (2) ◽  
pp. 315-324 ◽  
Author(s):  
P.C. Baciu ◽  
S. Saoncella ◽  
S.H. Lee ◽  
F. Denhez ◽  
D. Leuthardt ◽  
...  
Keyword(s):  
Plasma Membranes ◽  
Focal Adhesions ◽  
Cell Spreading ◽  
Cytoplasmic Domain ◽  
Cellular Protein ◽  
Actin Stress Fiber ◽  
Independent Manner ◽  
Intracellular Proteins ◽  
Central Regions ◽  
A Cell

Syndecan-4 is a cell surface heparan sulfate proteoglycan which, in cooperation with integrins, transduces signals for the assembly of focal adhesions and actin stress fibers in cells plated on fibronectin. The regulation of these cellular events is proposed to occur, in part, through the interaction of the cytoplasmic domains of these transmembrane receptors with intracellular proteins. To identify potential intracellular proteins that interact with the cytoplasmic domain of syndecan-4, we carried out a yeast two-hybrid screen in which the cytoplasmic domain of syndecan-4 was used as bait. As a result of this screen, we have identified a novel cellular protein that interacts with the cytoplasmic domain of syndecan-4 but not with those of the other three syndecan family members. The interaction involves both the membrane proximal and variable central regions of the cytoplasmic domain. We have named this cDNA and encoded protein syndesmos. Syndesmos is ubiquitously expressed and can be myristylated. Consistent with its myristylation and syndecan-4 association, syndesmos colocalizes with syndecan-4 in the ventral plasma membranes of cells plated on fibronectin. When overexpressed in NIH 3T3 cells, syndesmos enhances cell spreading, actin stress fiber and focal contact formation in a serum-independent manner.

scholarly journals Podophyllotoxin and Quercetin in Silico Derivatives Docking Analysis with Cyclooxygenase-2 and Aminopeptidase-N

2021 ◽  
Author(s):  
A.E. Manukyan ◽  
A.A. Hovhannisyan
Keyword(s):  
In Silico ◽  
Biological Activities ◽  
Three Dimensional ◽  
Aminopeptidase N ◽  
Docking Analysis ◽  
Quercetin Derivatives ◽  
Pubchem Database ◽  
A Cell ◽  
Cox 2 ◽  
High Level

ABSTRACTThe cyclooxygenase (COX) enzymes are tumor markers, the inhibition of which can be used in the prevention and therapy of carcinogenesis. It was found that COX-2 IS considered as targets for tumor inhibition. Aminopeptidase N (APN) is a type II membrane-bound metalloprotease associated with cancer, being identified as a cell marker on the surface of malignant myeloid cells and reached a high level of expression in progressive tumors. In anticancer therapy, plant compounds are considered that can inhibit their activity. Modeling of the COX-2 and APN enzymes was carried out on the basis of molecular models of three-dimensional structures from the PDB database [PDB ID: 5f19, 4fyq] RCSB. For docking analysis, 3D ligand models were created using MarvinSketch based on the PubChem database [CID: 5280343, 5281654]. In silico experiments, for the first time, revealed the possible interaction and inhibition of COX-2 and APN by quercetin and quercetin derivatives. Aspirin and Marimastat were taken to compare the results. Possible biological activities and possible side effects of the ligands have been identified.

Reliability-Based Assessment Method of Buried Pipeline at Fault Crossings

2020 ◽  
Author(s):  
Qian Zheng ◽  
Xiaoben Liu ◽  
Hong Zhang ◽  
Samer Adeeb
Keyword(s):  
Neural Network ◽  
Finite Element ◽  
Finite Element Model ◽  
Limit State ◽  
Element Model ◽  
Normal Operation ◽  
Operating Pressure ◽  
Peak Strain ◽  
Highly Nonlinear ◽  
Fault Displacement

Abstract The tectonic fault, which is one of the most common geohazards in field, poses great threat to buried pipe segments. Pipes will process to buckling or fracture due to large strain induced by continuously increasing ground displacements during earthquakes. Therefore, it is imperative to conduct safety analysis on pipes which are buried in seismic areas for the sake of ensuring normal operation. However, the highly nonlinearity of pipe response restricts the proceeding of reliability assessment. In this study, a hybrid procedure combining finite element method and artificial neural network is proposed for reliability-based assessment. First of all, the finite element model is developed on ABAQUS platform to simulate pipe response to strike-slip fault displacements. Thus, the strain demand value (the peak strain value obtained by finite element model in each design case) can be collected for database establishment, which is the preparation for neural network training. Thoroughness of the strain demand database can be achieved by a fully comprehensive calculation with consideration of influencing factors involving pipe diameter and wall thickness, operating pressure, magnitude of fault displacement, intersection angle between pipeline and fault plane, and characteristic value of backfill mechanics. Sequentially, Back Propagation Neural Network (BPNN) with double hidden layers is trained based on the developed database, and the surrogate strain demand prediction model can be obtained after accuracy verification. Hence, the strain-based limit state function can be respectively determined for tensile and compressive conditions. The strain capacity term is simply assumed based on published papers, the strain demand term is naturally superseded by the surrogate BPNN model, and Monte Carlo Simulation is employed to compute the probability of failure (POF). At last, the workability of the proposed approach is tested by a case study in which basic variables are referred to the Second West-to-East natural gas transmission pipeline project. It indicates that ANN is a good solver for reliability problems with implicit limit state functions especially for highly nonlinear problems. The proposed method is capable of computing POFs, which is an exploratory application for reliability research on pipes withstanding fault displacement loads.

Interaction of external head impact parameters on region and volume of strain for collisions in sport

2018 ◽  
Vol 233 (2) ◽  
pp. 258-267 ◽  
Author(s):  
R Anna Oeur ◽  
Michael D Gilchrist ◽  
Thomas Blaine Hoshizaki
Keyword(s):  
Brain Injuries ◽  
Element Model ◽  
Brain Regions ◽  
Peak Strain ◽  
Centre Of Gravity ◽  
Impact Parameters ◽  
Four Levels ◽  
Set Up ◽  
The Impact ◽  
Strain Responses

Collisions with the head are the primary cause of concussion in contact sports. Head impacts can be further characterized by velocity, striking mass, compliance, and location (direction). The purpose of this study was to describe the interaction effects of these parameters on peak strain in four brain regions and the volume of strain for collision impacts. A pendulum test set-up was used to deliver impacts to an adult Hybrid III headform according to four levels of mass (3, 9, 15, and 21 kg), four velocities (1.5, 3.0, 4.5, and 6.0 m/s), two impact locations (through the centre of gravity and a non-centre of gravity), and three levels of compliance simulating unprotected, helmeted, and well-padded conditions in sport. Headform accelerations were input into a brain finite element model to obtain peak strain in the frontal, temporal, parietal, and occipital lobes and the volume of the brain experiencing 0.10, 0.15, 0.20, and 0.25 strains. Centre-of-gravity impacts created the highest strains (peak and volume) under low compliance and non-centre-of-gravity impacts produced greater strain responses under medium and high compliance conditions. The temporal lobe was the region that consistently displayed the highest peak strains, which may be due to the proximity of the impact locations to this region. Interactions between mass and velocity displayed effects where the 9-kg mass had higher peak and volumes of strain than the 15-kg mass at velocities of 3.0 and 4.5 m/s. This study demonstrates the important role of interacting impact parameters on increasing strain responses that are relevant to the spectrum of diffuse brain injuries, including concussion.

Sign in / Sign up

Export Citation Format

Share Document